ALEXIS Biochemicals: Caspase-5 (active) (human) (recombinant)

ALX-201-094

Revised 03-May-06

Caspase-5 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Specification

EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:	~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:	In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P51878: Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500: Caspase-5 (mouse)

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