ALEXIS Biochemicals: Caspase-7 (active) (human) (recombinant)

ALX-201-061

Revised 21-Jul-08

Caspase-7 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Specification

EC:	3.4.22.60
SOURCE/HOST:	Produced in E. coli.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized.
RECONSTITUTION:	Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY:	~25’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate DEVD-pNA (pNA: p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION:	Useful in screening caspase inhibitors, studying enzyme regulation, determining specificity of caspase substrates or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P55210: Caspase-7 (human) (precursor)
AfCS Signalling Gateway link A000502: Caspase-7 (mouse)

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