ALEXIS Biochemicals: Caspase-9 (active) (human) (recombinant)

ALX-201-136

Revised 14-Aug-08

Caspase-9 (active) (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspases

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-136-C005		5 µg	255.00 USD

Product Specification

EC:	3.4.22.-
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	0.2mg/ml
FORMULATION:	Liquid. In 50mM TRIS (pH 8.0) containing 100mM sodium chloride and 50mM imidazole.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least one week when stored at +4°C.
HANDLING:	Avoid freeze/thaw cycles.

Product Specific Literature References

A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis: N.A. Thornberry, et al.; J. Biol. Chem. 272, 17907 (1997) Abstract; Full Text

General Information

When expressed in E. coli, caspase-9 spontaneously undergoes autoprocessing to yield the subunits characteristic of the active enzyme. The rate of caspase-9 enzymatic activity can be measured by the release of AMC from the caspase substrate Ac-LEHD-AMC (Prod. No. ALX-260-080) as emission at 440nm and excitation at 380nm using a spectrofluorometer.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P55211: Caspase-9 (human) (precursor) AfCS Signalling Gateway link A000504: Caspase-9 (mouse)

Further Categories Containing This Product:

Enzymes • Recombinant Proteins/Fusion Proteins

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