ALEXIS Biochemicals: Caspase Fluorometric Substrate Set

ALX-850-229

Revised 29-May-08

Caspase Fluorometric Substrate Set
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Kits & Sets

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-229-KI01		1 Set	553.00 USD

Product Specification

KIT/SET CONTAINS:	Contains 125µl (1mM) each of the following substrates: - Caspase-1 Substrate, Ac-YVAD-AFC - Caspase-2 Substrate, Ac-VDVAD-AFC - Caspase-3 Substrate, Ac-DEVD-AFC - Caspase-5 Substrate, Ac-WEHD-AFC - Caspase-6 Substrate, Ac-VEID-AFC - Caspase-8 Substrate, Ac-IETD-AFC - Caspase-9 Substrate, Ac-LEHD-AFC
FORMULATION:	Liquid. Ready-to-use in DMSO.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C

Product Description

Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.

General Information

BACKGROUND/TECHNICAL INFORMATION

Assay Procedure:
1. Induce apoptosis in cells by desired method. Concurrently incubate a control culture without induction.
2. Count cells and pellet 1-5x10⁶ cells or use 50-200µg cell lysates if protein concentration has been
    measured.
3. Resuspend cells in 50µl of chilled Cell Lysis Buffer.
4. Incubate cells on ice for 10 minutes.
5. Add 50µl of 2x Reaction Buffer containing 10mM DTT to each sample.
6. Add 5µl of the 1mM AFC conjugated substrates (50µM final conc.) into each tube individually and
    incubate at 37°C for 1-2 hours.
7. Read samples in a fluorometer equipped with a 400-nm excitation filter and a 505-nm emission filter.
    For a plate-reading set-up, transfer the samples to a 96-well plate. You may perform the entire assay
    directly in a 96 well plate.

    Fold-increase in caspase activity can be determined by comparing these results with the level of the
    uninduced control.

    Note: Background reading from cell lysates and buffers should be subtracted from the readings of both
    induced and the uninduced samples before calculating fold increase in caspase activity.

MANUFACTURER

Manufactured by BioVision.

Further Categories Containing This Product:

Caspase Substrates • Panels / Sets

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