ALEXIS Biochemicals: Cathepsin L (human)

ALX-200-314

Revised 27-Jun-06

Cathepsin L (human)
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Cathepsins

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-200-314-1		1 Vial	270.00 USD

Product Specification

MW:	~29kDa.
EC:	3.4.22.15
MERCK INDEX:	14: 1905
SOURCE/HOST:	Isolated from human liver. Negative for HBSAG-, HCV- and HIV-antibodies.
QUANTITY:	25µg
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM malonate buffer, pH 5.5, containing 1mM EDTA and 400mM sodium chloride.
SPECIFIC ACTIVITY:	≥0.5U/mg protein. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Z-Phe-Arg-AFC (Prod. No. ALX-260-129) per min. at 25°C in 400mM Na acetate, pH 5.5 with 4mM EDTA and 8mM DTT.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C

Product Description

The most powerful of the lysosomal proteinases. It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates.

Product Specific Literature References

Cathepsin B, Cathepsin H, and cathepsin L: A.J. Barrett & H. Kirschke; Methods Enzymol. 80 Pt C, 535 (1981) Abstract
Active center differences between cathepsins L and B: the S1 binding region: H. Kirschke, et al.; FEBS Lett 228, 128 (1988) Abstract
Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L. An abundant transcript induced by transformation of fibroblasts: L.J. Joseph, et al.; J. Clin. Invest. 81, 1621 (1988) Abstract
The role of lysosomal proteinases in MHC class II-mediated antigen processing and presentation: T.Y. Nakagawa & A.Y. Rudensky; Immunol. Rev. 172, 121 (1999) Abstract
Proteases involved in MHC class II antigen presentation: J.A. Villadangos, et al.; Immunol. Rev. 172, 109 (1999) Abstract
Distinct roles of cathepsin K and cathepsin L in osteoclastic bone resorption: N. Furuyama & Y. Fujisawa; Endocr. Res. 26, 189 (2000) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

May contain trace cathepsin B contaminant (<1.5U/mg protein using Z-Arg-Arg-β-NA as a substrate. One unit is defined as the amount of enzyme that hydrolyzes 1µmol of 2-naphtylamine per min. at 40°C, pH 6.0. Please note that the specific activity of pure cathepsin B is >200U/mg protein)

Swiss-Prot link P07711: Cathepsin L (human) (precursor)
AfCS Signalling Gateway link A000515: Cathepsin L (mouse)

Further Categories Containing This Product:

Natural Proteins • Enzymes

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