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ALX-380-219 Revised 08-Apr-08
Reveromycin D
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics for Cell Cycle Research
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ALX-380-219-MC25   0.25 mg 320.00 USD Add To Cart
Product Specification
FORMULA: C37H54O11
MW: 674.8
CAS NUMBER: 144860-70-8
SOURCE/HOST: Isolated from Streptomyces sp. MST-AS5344.
PURITY: ≥95% (HPLC)
APPEARANCE: White to tan solid.
SOLUBILITY: Soluble in ethyl acetate, 100% ethanol, methanol, dimethyl formamide or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Unstable in acidic conditions.

Product Description
Antibiotic. Minor component of the reveromycin complex. Inhibitor of the mitogenic activity of epidermal growth factor (EGF). More active than its straight chain analog reveromycin C (Prod. No. ALX-380-218).
Product Specific Literature References
Reveromycins, new inhibitors of eukaryotic cell growth. I. Producing organism, fermentation, isolation and physico-chemical properties: H. Takahashi, et al.; J. Antibiot. (Tokyo) 45, 1409 (1992) Abstract
Reveromycins, new inhibitors of eukaryotic cell growth. II. Biological activities: H. Takahashi, et al.; J. Antibiot. (Tokyo) 45, 1414 (1992) Abstract
 
 
ALX-380-071 Revised 12-Oct-07
Rifampicin
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SYNONYMS Rifampin
3-(4-Methylpiperazinyl-iminomethyl)rifamycin SV
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics for Cell Cycle Research
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ALX-380-071-M100   100 mg 18.00 USD Add To Cart
Product Specification
FORMULA: C43H58N4O12
MW: 823.0
CAS NUMBER: 13292-46-1
MERCK INDEX: 14: 8216
SOURCE/HOST: Semisynthetic.
PURITY: ≥95%
SOLUBILITY: Soluble in chloroform or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Antibacterial (tuberculostatic). Inhibits DNA-dependent RNA polymerase. Potent inducer of hepatic cytochrome P450 enzymes.
Product Specific Literature References
Rifampicin: a new orally active rifamycin: N. Maggi, et al.; Chemotherapy 11, 285 (1966) Abstract
Rifampicin sensitivity of the components of DNA-dependent RNA polymerase: E. di Mauro, et al.; Nature 222, 533 (1969) Abstract
Chemical and biological properties of rifampicin: S. Furesz; Antibiot. Chemother. 16, 316 (1970) Abstract
On the mechanism of rifampicin inhibition of RNA synthesis: W.R. McClure and C.L. Cech; J. Biol. Chem. 253, 8949 (1978) Abstract
Pharmacokinetic drug interactions with rifampicin: K. Venkatesan; Clin. Pharmacokinet. 22, 47 (1992), Review Abstract
Pharmacokinetic interactions with rifampicin : clinical relevance: M. Niemi, et al.; Clin. Pharmacokinet. 42, 819 (2003), Review Abstract
Roles of rifampicin in drug-drug interactions: underlying molecular mechanisms involving the nuclear pregnane X receptor: J. Chen and K. Raymond; Ann. Clin. Microbiol. Antimicrob. 50, 3 (2006), Review Abstract
 
 
ALX-430-151 Revised 22-Jul-08
RITA
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SYNONYMS Reactivation of p53 and Induction of Tumor Cell Apoptosis
2,5-bis(5-Hydroxymethyl-2-thienyl)furan
NSC652287
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-430-151-M001   1 mg 75.00 USD Add To Cart
ALX-430-151-M005   5 mg 290.00 USD Add To Cart
Product Specification
FORMULA: C14H12O3S2
MW: 292.4
CAS NUMBER: 213261-59-7
PURITY: ≥98%
APPEARANCE: White to yellow crystalline solid.
SOLUBILITY: Soluble in 100% ethanol (1mg/ml), DMSO (20mg/ml), dimethyl formamide (30mg/ml); 0.5mg/ml soluble in a 1:1 solution of dimethyl formamide: PBS (pH 7.2) (dilute the dimethyl formamide solution with PBS).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least two years after receipt when stored at -20°C. We do not recommend storing aqueous solutions for more than one day.

Product Description
Inhibitor of p53/HDM2 interaction. Activator of p53 function in tumors. Potently inhibits the growth of a variety of  cancer cell lines in the submicromolar range. It has been suggested that RITA selectively targets cells expressing p53 by other mechanisms than direct binding to the N-terminal domain of p53 [4].
Product Specific Literature References
[1] DNA protein cross-links produced by NSC 652287, a novel thiophene derivative active against human renal cancer cells: W. Nieves-Neira, et al.; Mol. Pharmacol. 56, 478 (1999) Abstract; Full Text
[2] Selective toxicity of the tricyclic thiophene NSC 652287 in renal carcinoma cell lines: differential accumulation and metabolism: M.I. Rivera, et al.; Biochem. Pharmacol. 57, 1283 (1999) Abstract
[3] Small molecule RITA binds to p53, blocks p53-HDM-2 interaction and activates p53 function in tumors: N. Issaeva, et al.; Nat. Med. 10, 1321 (2004) Abstract
[4] NMR indicates that the small molecule RITA does not block p53-MDM2 binding in vitro: M. Krajewski, et al.; Nat. Med. 11, 1135 (2005) Abstract
Further Categories Containing This Product:
Antitumor Agents (Apoptosis Inducers)
 
 
ALX-270-463 Revised 22-Apr-08
RO-3306
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SYNONYMS (5Z)-2-((Thiophen-2-yl)methylamino)-5-((quinolin-6-yl)methylene)thiazol-4(5H)-one
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Inhibitors
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ALX-270-463-M001   1 mg 50.00 USD Add To Cart
ALX-270-463-M005   5 mg 200.00 USD Add To Cart
Product Specification
FORMULA: C18H13N3OS2
MW: 351.4
CAS NUMBER: 872573-93-8
PURITY: ≥95% (1H-NMR)
APPEARANCE: Brown solid.
SOLUBILITY: Soluble in DMSO (5mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C in the dark.
HANDLING: Protect from light.
IDENTITY: Identity determined by 1H-NMR, 13C-NMR and MS.

Product Description
Inhibitor of CDK1/cyclin B1 and CDK1/cyclin A. Reversibly arrests human cells at the G2/M border of the cell cycle. Allows for effective cell synchronisation in early mitosis.
Product Specific Literature References
Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1: L.T. Vassilev, et al.; PNAS 103, 10660 (2006) Abstract; Full Text
Cell cycle synchronization at the G2/M phase border by reversible inhibition of CDK1: L.T. Vassilev; Cell Cycle 5, 2555 (2006) Abstract; Full Text
Further Categories Containing This Product:
Cell Cycle Blockers & Inhibitors / Related Products
 
 
ALX-350-251 Revised 23-Oct-07
(R)-Roscovitine
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SYNONYMS 6-Benzylamino-2-(R)-[(1-ethyl)-2-hydroxyethylamino]-9-isopropylpurine
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Inhibitors
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-251-M001   1 mg 30.00 USD Add To Cart
ALX-350-251-M005   5 mg 110.00 USD Add To Cart
ALX-350-251-M050   50 mg 450.00 USD Add To Cart
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Product Specification
FORMULA: C19H26N6O
MW: 354.5
CAS NUMBER: 186692-46-6
PURITY: ≥97%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in chloroform, DMSO or methanol; moderately soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
Potent and selective inhibitor of CDKs. Selective for CDK1/cyclin B kinase (IC50=450nM) [2], CDK2 (IC50=700nM) [4] and CDK5/p35 (IC50=160nM) [4,5]. More potent than olomoucine (Prod. No. ALX-350-013). Inhibits M phase promoting factor (MPF) kinase activity. Has effects on calcium channel gating.
Product Specific Literature References
[1] Activation of cyclin-dependent kinases by Myc mediates induction of cyclin A, but not apoptosis: B. Rudolph, et al.; EMBO J. 15, 3065 (1996) Abstract
[2] Inhibition of cyclin-dependent kinases by purine analogues: crystal structure of human cdk2 complexed with roscovitine: W.F. De Azvedo, et al.; Eur. J. Biochem. 243, 518 (1997) Abstract
[3] Cytokinin-derived cyclin-dependent kinase inhibitors: synthesis and cdc2 inhibitory activity of olomoucine and related compounds: L. Havlicek, et al.; J. Med. Chem. 40, 408 (1997) Abstract
[4] Chemical inhibitors of cyclin-dependent kinases: L. Meijer and S.H. Kim; Meth. Enzymol. 283, 113 (1997) Abstract
[5] Biochemical and cellular effects of roscovitine, a potent and selective inhibitor of the cyclin-dependent kinases cdc2, cdk2 and cdk5: L. Meijer, et al.; Eur. J. Biochem. 243, 527 (1997) Abstract
[6] Direct in vivo inhibition of the nuclear cell cycle cascade in experimental mesangial proliferative glomerulonephritis with Roscovitine, a novel cyclin-dependent kinase antagonist: J.W. Pippin, et al.; J. Clin. Invest. 100, 2512 (1997) Abstract
[7] Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine: J.S. Yakisich, et al.; BBRC 243, 674 (1998) Abstract
[8] The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity: F. Alessi, et al.; Exp. Cell Res. 245, 8 (1998) Abstract
[9] Effects of cyclin-dependent kinase inhibitors on transcription and ocular circadian rhythm of Aplysia: N. Sankrithi & A. Eskin; J. Neurochem. 72, 605 (1999) Abstract
[10] Transcription of herpes simplex virus immediate-early and early genes is inhibited by roscovitine, an inhibitor specific for cellular cyclin- dependent kinases: L.M. Schang, et al.; J. Virol. 73, 2161 (1999) Abstract
[11] Roscovitine and other purines as kinase inhibitors. From starfish oocytes to clinical trials: L. Meijer & E. Raymond; Acc. Chem. Res. 36, 417 (2003), (Review) Abstract
[12] The specificities of protein kinase inhibitors: an update: J. Bain, et al.; Biochem. J. 371, 199 (2003) Abstract
[13] Roscovitine, olomoucine, purvalanol: inducers of apoptosis in maturing cerebellar granule neurons: E.A. Monaco, 3rd, et al.; Biochem. Pharmacol. 67, 1947 (2004) Abstract
[14] Roscovitine targets, protein kinases and pyridoxal kinase: S. Bach, et al.; J. Biol. Chem. 280, 31208 (2005) Abstract
[15] The effects of presynaptic calcium channel modulation by roscovitine on transmitter release at the adult frog neuromuscular junction: S. Cho & S.D. Meriney; Eur. J. Neurosci. 23, 3200 (2006) Abstract
[16] Roscovitine differentially affects CaV2 and Kv channels by binding to the open state: Z. Buraei, et al.; Neuropharmacology 52, 883 (2007) Abstract
Further Categories Containing This Product:
Ion Channels & Transporters Other Products
 
 
ALX-350-293 Revised 19-Oct-07
(S)-Roscovitine
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SYNONYMS 6-Benzylamino-2-(S)-[(1-Ethyl)-2-hydroxyethylamino]-9-isopropylpurine
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Inhibitors
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ALX-350-293-M001   1 mg 40.00 USD Add To Cart
Product Specification
FORMULA: C19H26N6O
MW: 354.5
CAS NUMBER: 186692-46-6
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, dimethyl formamide, 100% ethanol or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C

Product Description
(S)-Enantiomer of the CDK inhibitor roscovitine (Prod. No. ALX-350-251). Potent inhibitor of CDK1/cyclin B kinase (IC50=950nM) [1].
Product Specific Literature References
[1] Inhibition of cyclin-dependent kinases by purine analogues: crystal structure of human cdk2 complexed with roscovitine: W.F. De Azevedo, et al.; Eur. J. Biochem. 243, 518 (1997) Abstract
[2] Cytokinin-derived cyclin-dependent kinase inhibitors: synthesis and cdc2 inhibitory activity of olomoucine and related compounds: L. Havlicek, et al.; J. Med. Chem. 40, 408 (1997) Abstract
[3] The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity: F. Alessi, et al.; Exp. Cell Res. 245, 8 (1998) Abstract
Maintenance of meiotic arrest in bovine oocytes using the S-enantiomer of roscovitine: effects on maturation, fertilization and subsequent embryo development in vitro: P. Coy, et al.; Reproduction 129, 19 (2005) Abstract
In vitro maturation and fertilization of porcine oocytes after a 48 h culture in roscovitine, an inhibitor of p34cdc2/cyclin B kinase: R. Romar & H. Funahashi; Anim. Reprod. Sci. 92, 321 (2006) Abstract
 
 
ALX-380-067 Revised 08-Apr-08
β-Rubromycin
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antibiotics - Antiviral / Anti-HIV
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ALX-380-067-M001   1 mg 40.00 USD Add To Cart
ALX-380-067-M005   5 mg 160.00 USD Add To Cart
Product Specification
FORMULA: C27H20O12
MW: 536.5
SOURCE/HOST: Isolated from Streptomyces sp.
PURITY: ≥99%
SOLUBILITY: Soluble in chloroform.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: TOXIC.

Product Description
Antibiotic. Inhibitor of HIV-1 reverse transcriptase (RT). Inhibits human telomerase. Cytostatically active against different tumor cell lines.
Product Specific Literature References
The structure of rubromycin: H. Brockmann, et al.; THL 30, 3525 (1966), (Article in German) Abstract
Rubromycin II: H. Brockmann, et al.; Chem. Ber. 102, 126 (1969) Abstract
Rubromycins. 3. The constitution of alpha-rubromycin, beta-rubromycin, gamma-rubromycin, and gamma-iso-rubromycin: H. Brockmann & A. Zeeck; Chem. Ber. 103, 1709 (1970) Abstract
Inhibition of human immunodeficiency virus-1 reverse transcriptase activity by rubromycins: competitive interaction at the template.primer site: M.E. Goldman, et al.; Mol. Pharmacol. 38, 20 (1990) Abstract
Inhibition of human telomerase by rubromycins: implication of spiroketal system of the compounds as an active moiety: T. Ueno, et al.; Biochemistry 39, 5995 (2000) Abstract
Structural and Biosynthetic Investigations of the Rubromycins: C. Puder, et al.; Eur. J. Org. Chem. 2000, 729 (2000)
 
 
ALX-201-426 Revised 24-Jul-08 New product
Sirtuin 1 (human) (recombinant) (His)
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SYNONYMS Sirt1 (human) (recombinant) (His)
SIR2-like Protein 1 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Sirtuins / Related Products
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ALX-201-426-C010   10 µg 300.00 USD Add To Cart
ALX-201-426-C050   50 µg 470.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. The mature peptide of human sirtuin 1 (aa 2-555) is fused at the N-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM sodium chloride.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q96EB6: SIRT1 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
 
 
ALX-201-430 Revised 12-Aug-08 New product
Sirtuin 2 (human) (recombinant) (His)
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SYNONYMS Sirt2 (human) (recombinant) (His)
SIR2-like Protein 2 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Sirtuins / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-430-C010   10 µg 300.00 USD Add To Cart
ALX-201-430-C050   50 µg 470.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. The mature peptide of human sirtuin 2 (aa 2-389) is fused at the N-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2μm-filtered solution in 50mM TRIS-Cl, pH 8.2, containing 100mM sodium chloride.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q8IXJ6: SIRT2 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
 
 
ALX-303-003 Revised 29-Jan-05
D-erythro-Sphingosine, N-Hexanoyl-