ALEXIS Biochemicals: QuickCount™ Cell Quantification Kit

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ALX-850-039

Revised 20-Dec-07

Cell Counting Kit-8
SYNONYMS	CCKi-8
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	Cell Proliferation & Cytotoxicity Assays

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-039-KI01		500 tests	150.00 USD
ALX-850-039-KI02		5x500 tests	450.00 USD

Product Specification

QUANTITY:	500 tests/5ml; 2500 tests/5 x 5ml.
KIT/SET CONTAINS:	5mM WST-8 solution, 0.2mM 1-methoxy-PMS, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles. Protect from light.

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Product Description

Colorimetric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages: One-step, ready-to-use solution, no radioisotopes, no solubilization step, high sensitivity, correlates with the [³H]-thymidine incorporation assay. Can be added directly to the cell media for fast, high-throughput screening without a solubilization process obtaining highly reproducible and accurate results.

Product Specific Literature References

A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet: M. Ishiyama, et al.; Biol. Pharm. Bull. 19, 1518 (1996) Abstract
Antenatal hydronephrosis. Fetal and neonatal management: J.S. Elder; Pediatr. Clin. North Am. 44, 1299 (1997) Abstract
A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability: M. Ishiyama, et al.; Talanta 44, 1299 (1997)
Enhancement of MTT, a tetrazolium salt, exocytosis by amyloid beta- protein and chloroquine in cultured rat astrocytes: I. Isobe, et al.; Neurosci. Lett. 266, 129 (1999) Abstract
A water-soluble tetrazolium salt useful for colorimetric cell viability assay: H. Tominaga, et al.; Anal. Commun. 36, 47 (1999)
Increased cytotoxicity of cadmium in fibroblasts lacking c-fos: M. Matsuoka, et al.; Biochem. Pharmacol. 59, 1573 (2000) Abstract
Mitogen-activated protein kinases mediate peroxynitrite-induced cell death in human bronchial epithelial cells: E. Nabeyrat, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 284, L1112 (2003) Abstract; Full Text
Cyclin G2 dysregulation in human oral cancer: Y. Kim, et al.; Cancer Res. 64, 8980 (2004) Abstract; Full Text
Mutation of Cys105 inhibits dimerization of p12CDK2-AP1 and its growth suppressor effect: Y. Kim, et al.; J. Biol. Chem. 280, 23273 (2005) Abstract; Full Text

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ALX-850-245

Revised 03-Feb-05

Cell Counting Kit-F
SYNONYMS	CCKi-F
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	Cell Proliferation & Cytotoxicity Assays

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-245-KI01		500 tests	215.00 USD
ALX-850-245-KI02		5x500 tests	790.00 USD

Product Specification

SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Fluorometric kit for simple and accurate cell proliferation and cytotoxicity assays. Advantages: No radioisotopes, more sensitive than the colorimetric assay, short staining reaction time, no solubilization step, correlates with the [³H]-thymidine incorporation assay.

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ALX-850-249

Revised 20-May-08

Live-Dead Cell Staining Kit
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	Cell Proliferation & Cytotoxicity Assays

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-249-KI01		1 Kit	273.00 USD

Product Specification

QUANTITY:	100 tests
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light.

Product Description

Kit utilizes Live-dye™, a cell permeable green fluorescent dye (Ex(max): 488nm; Em(max): 518nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a red fluorescent dye (Ex(max): 488nm; Em(max): 615nm), which in viable cells is actively pumped out of the cytoplasm. Stained cells can be visualized by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine). Cyto-dye has also been used to discriminate between apoptotic and non-apoptotic cells. Ready-to-use.

General Information

MANUFACTURER

Manufactured by BioVision.

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ALX-850-308

Revised 23-Nov-05

QuickCount™ Cell Quantification Kit
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	Cell Proliferation & Cytotoxicity Assays

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-308-KI01		1 Kit	200.00 USD

Product Specification

KIT/SET CONTAINS:	2x75ml of lysis buffer and 2x0.075ml of dye reagent.
APPLICATION:	For assessing the number of cells present in a wide range of cell concentrations.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+20°C
USE/STABILITY:	Store dye reagent at -20°C.

Product Description

Suited for high throughput screening and includes protocols for adherent and suspended cells. Flexible to use. Can be optimized in formats ranging from 96 wells to cuvettes. Nonradioactive. Can also be used in time course studies to measure cell proliferation, and for the quantification of double stranded DNA in a sample of plasmid, lamda or genomic DNA. Excitation at 355nm and Emission at 460nm.

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