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ALX-350-014
Revised 03-Apr-08
Calyculin A
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Natural Products - Other Signal Transduction Pathway Modulators
Ordering Information
Product Numbers:
Format:
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Unit Price:
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ALX-350-014-C010
10 µg
65.00 USD
ALX-350-014-C025
25 µg
110.00 USD
ALX-350-014-C050
50 µg
190.00 USD
ALX-350-014-C100
100 µg
320.00 USD
Product Specification
FORMULA:
C
50
H
81
N
4
O
15
P
MW:
1009.2
CAS NUMBER:
101932-71-2
SOURCE/HOST:
Isolated from
Discodermia calyx
.
PURITY:
≥95%
APPEARANCE:
Lyophilized.
SOLUBILITY:
Soluble in DMSO, 100% ethanol, methanol, acetonitrile, chloroform, methylene chloride or benzene. Insoluble in water.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
May by subject to isomerization when in solution. Biological activity is not affected by isomerization.
HANDLING:
Protect from light and moisture.
HAZARD:
TOXIC. MAY BE CARCINOGENIC.
Product Description
Potent cell permeable inhibitor with high specificity for protein phosphatase 1 (PP1) and 2A (PP2A). Inhibits p130cas tyrosine phosphorylation. Enhances the phosphorylation level of NF-κB. Affects intracellular signalling processes that require 14-3-3. Potent non-phorbol type tumor promoter. Prevents γ-radiation induced apoptosis.
Product Specific Literature References
Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter:
M. Suganuma, et al.; PNAS
85
, 1768 (1988)
Abstract
Protein phosphatases come of age:
P.Cohen & P.T. Cohen; J. Biol. Chem.
264
, 21435 (1989)
Abstract
;
Full Text
Calyculin A and okadaic acid: inhibitors of protein phosphatase activity:
H. Ishihara, et al.; BBRC
159
, 871 (1989)
Abstract
The structure and regulation of protein phosphatases:
P. Cohen; Ann. Rev. Biochem.
58
, 453 (1989)
Abstract
Calyculin A, an inhibitor of protein phosphatases, a potent tumor promoter on CD-1 mouse skin:
M. Suganuma, et al.; Cancer Res.
50
, 3521 (1990)
Abstract
Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C:
R. Gopalakrishna; BBRC
189
, 950 (1992)
Abstract
Comparative effects of protein phosphatase inhibitors (okadaic acid and calyculin A) on human leukemia HL60, HL60/ADR and K562 cells:
K. Sakurada, et al.; BBRC
187
, 488 (1992)
Abstract
Calyculin A, a potent inhibitor of phosphatases-1 and -2A, prevents apoptosis:
Q. Song & M.F. Lavin; BBRC
190
, 47 (1993)
Abstract
Enhancement of AMPA-mediated synaptic transmission by the protein phosphatase inhibitor calyculin A in rat hippocampal slices:
A. Figurov, et al.; Eur. J. Neurosci.
5
, 1035 (1993)
Abstract
Calyculin A, a non-phorbol ester type tumor promotor, induced oxidative DNA damage in stimulated human neutrophil-like cells:
T. Takeuchi, et al.; BBRC
205
, 1803 (1994)
Abstract
Potassium and calcium channel involvement in induction of long-lasting synaptic enhancement by calyculin A, a protein phosphatase inhibitor, in rat hippocampal CA1 region:
N. Murakami, et al.; Neurosci. Lett.
176
, 181 (1994)
Abstract
Differential toxicity of the protein phosphatase inhibitors microcystin and calyculin A:
M.T. Runnegar, et al.; J. Pharmacol. Exp. Ther.
273
, 545 (1995)
Abstract
Inhibition of p130cas tyrosine phosphorylation by calyculin A:
W. Qiu, et al.; J. Leukoc. Biol.
63
, 631 (1998)
Abstract
Unique features of the okadaic acid activity class of tumor promoters:
H. Fujiki & M. Suganuma; J. Cancer Res. Clin. Oncol.
125
, 150 (1999), Review
Abstract
Calyculin A-induced vimentin phosphorylation sequesters 14-3-3 and displaces other 14-3-3 partners in vivo:
G. Tzivion, et al.; J. Biol. Chem.
275
, 29772 (2000)
Abstract
Calyculin A induces apoptosis and stimulates phosphorylation of p65NF-kappaB in human osteoblastic osteosarcoma MG63 cells:
H. Tanaka, et al.; Int. J. Oncol.
31
, 389 (2007)
Abstract
Further Categories Containing This Product:
14-3-3 Proteins / Related Products
•
Carcinogens & Tumor Promoters Other Products
•
Natural Products - Other Tumor Promoters
•
NF-kB Pathway Modulators
•
PP1 / Related Products
•
PP2A / Related Products
•
Marine Natural Products
•
Natural Products - Apoptosis Inducers & Inhibitors
•
Autophagy Blockers & Inhibitors / Related Products
ALX-270-062
Revised 03-Feb-05
Cantharidic acid . disodium salt
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PP2A / Related Products
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ALX-270-062-M010
10 mg
110.00 USD
Product Specification
FORMULA:
C
10
H
12
O
5
. 2Na
MW:
212.2 . 46.0
CAS NUMBER:
28874-45-5
PURITY:
≥95%
APPEARANCE:
White solid.
SOLUBILITY:
Soluble in water; slightly soluble in DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
Product Description
Potent protein phosphatase 2A inhibitor.
Product Specific Literature References
M. Matsuzawa, et al.; J. Agric. Food Chem.
35
, 823 (1987)
Partial characterization of specific cantharidin binding sites in mouse tissues
:
M.J. Graziano, et al.; Mol. Pharmacol.
33
, 706 (1989)
Abstract
Cantharidin-binding protein: identification as protein phosphatase 2A:
Y.-M. Li & J.E. Casida; PNAS
89
, 11867 (1992)
Abstract
ALX-270-063
Revised 03-Apr-08
Cantharidin
SYNONYMS
Hexahydro-3a,7a-dimethyl-4,7-epoxyisobenzo-furan-1,3-dione
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Natural Products - Other Signal Transduction Pathway Modulators
Ordering Information
Product Numbers:
Format:
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ALX-270-063-M025
25 mg
50.00 USD
ALX-270-063-M100
100 mg
160.00 USD
Product Specification
FORMULA:
C
10
H
12
O
4
MW:
196.2
CAS NUMBER:
56-25-7
MERCK INDEX:
14:
1752
PURITY:
≥98%
APPEARANCE:
White to off-white solid.
SOLUBILITY:
Soluble in acetone, DMSO or 100% ethanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
HANDLING:
Protect from light.
HAZARD:
TOXIC.
Product Description
Potent protein phosphatase 2A (PP2A) inhibitor. At higher concentrations, also inhibits protein phosphatase 1 (PP1).
Product Specific Literature References
Partial characterization of specific cantharidin binding sites in mouse tissues
:
M.J. Graziano, et al.; Mol. Pharmacol.
33
, 706 (1988)
Abstract
Cantharidin-binding protein: identification as protein phosphatase 2A:
Y.-M. Li and J.E. Casida; PNAS
89
, 11867 (1992)
Abstract
Protein phosphatase 2A and its [3H]cantharidin/[3H]endothall thioanhydride binding site. Inhibitor specificity of cantharidin and ATP analogues:
Y.-M. Li, et al.; Biochem. Pharmacol.
46
, 1435 (1993)
Abstract
Further Categories Containing This Product:
PP2A / Related Products
•
Autophagy Blockers & Inhibitors / Related Products
ALX-380-065
Revised 24-Sep-08
Fostriecin
SYNONYMS
Phosphotrienin
CI-920
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Antibiotics - Topoisomerase Inhibitors
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ALX-380-065-C010
10 µg
150.00 USD
Product Specification
FORMULA:
C
19
H
26
O
9
PNa
MW:
452.4
CAS NUMBER:
87860-39-7
RTECS:
UQ0600000
SOURCE/HOST:
Isolated from
Streptomyces pulveraceous
subsp.
fostreus
.
PURITY:
≥98% (HPLC)
APPEARANCE:
Colorless solid.
SOLUBILITY:
Soluble in water (50mg/ml), methanol (10mg/ml) or 100% ethanol (10mg/ml).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year when stored at -20°C. Use fresh solution.
HANDLING:
Protect from light. Packaged under inert gas. Hygroscopic.
Product Description
Antitumor antibiotic. Catalytic inhibitor of topoisomerase II. Strong protein phosphatase 2A (PP2A) inhibitor (IC
50
=3.2nM). Weak inhibitor for PP1 (IC
50
=131µM). No apparent effect on PP2B. The binding site for fostriecin on PP2A is different from that of okadaic acid (see Prod. No.
ALX-350-003
).
Product Specific Literature References
Novel antitumor agents CI-920, PD 113,270 and PD 113,271. II. Isolation and characterization:
S.S. Stampwala, et al.; J. Antibiot. (Tokyo)
36
, 1601 (1983)
Abstract
Novel antitumor agents CI-920, PD 113,270 and PD 113,271. I. Taxonomy, fermentation and biological properties:
J.B. Tunac, et al.; J. Antibiot. (Tokyo)
36
, 1595 (1983)
Abstract
Studies on the biochemical mechanism of the novel antitumor agent, CI- 920:
D.W. Fry, et al.; Cancer Chemother. Pharmacol.
13
, 171 (1984)
Abstract
Anticancer activity of the structurally novel antibiotic Cl-920 and its analogues:
W.R. Leopold, et al.; Cancer Res.
44
, 1928 (1984)
Abstract
Inhibition of type II topoisomerase by fostriecin:
T.J. Boritzki, et al.; Biochem. Pharmacol.
37
, 4063 (1988)
Abstract
Comparison of effects of fostriecin, novobiocin, and camptothecin, inhibitors of DNA topoisomerases, on DNA replication and repair in human cells:
C.M. Gedik & A.R. Collins; Nucl. Acids Res.
18
, 1007 (1990)
Abstract
The cell cycle related differences in susceptibility of HL-60 cells to apoptosis induced by various antitumor agents:
W. Gorczyca, et al.; Cancer Res.
53
, 3186 (1993)
Abstract
Induction of DNA strand breaks associated with apoptosis during treatment of leukemias:
W. Gorczyca, et al.; Leukemia
7
, 659 (1993)
Abstract
Antitumor drug fostriecin inhibits the mitotic entry checkpoint and protein phosphatases 1 and 2A:
M. Roberge, et al.; Cancer Res.
54
, 6115 (1994)
Abstract
Fostriecin: a review of the preclinical data:
R.S. de Jong, et al.; Anticancer Drugs
8
, 413 (1997)
Abstract
Fostriecin, an antitumor antibiotic with inhibitory activity against serine/threonine protein phosphatases types 1 (PP1) and 2A (PP2A), is highly selective for PP2A:
A.H. Walsh, et al.; FEBS Lett.
416
, 230 (1997)
Abstract
Fostriecin, an inhibitor of protein phosphatase 2A, limits myocardial infarct size even when administered after onset of ischemia:
C. Weinbrenner, et al.; Circulation
98
, 899 (1998)
Abstract
Phase I and pharmacokinetic study of the topoisomerase II catalytic inhibitor fostriecin:
R.S. de Jong, et al.; Br. J. Cancer
79
, 882 (1999)
Abstract
Fostriecin: chemistry and biology:
D.S. Lewy, et al.; Curr. Med. Chem.
9
, 2005 (2002)
Abstract
Further Categories Containing This Product:
Antibiotics - Other Signal Transduction Pathway Modulators
•
Antitumor Antibiotics
•
Antitumor Agents (Enzyme Inhibitors)
•
PP2A / Related Products
ALX-804-636
Revised 31-Oct-06
Monoclonal Antibody to PP2A (Subunit A) (PR65) (α Isoform) (4C10)
SYNONYMS
anti-Protein Phosphatase 2A (Subunit A) (PR65) (α Isoform) MAb (4C10)
anti-PP2A A/PR65α MAb (4C10)
anti-Medium Tumor Antigen-associated 61kDa Protein MAb (4C10)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PP2A / Related Products
Ordering Information
Product Numbers:
Format:
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Quantity:
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ALX-804-636-C050
50 µg
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
4C10
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human PP2A (subunit A) (PR65) (α isoform) (aa 1-168).
SPECIFICITY:
Recognizes human and mouse PP2A (subunit A) (PR65) (α isoform). Recognizes mouse PP2A (subunit A) (PR65) (β isoform) (weak).
CROSSREACTIVITY PROTEINS:
PP2A (subunit A) (PR65) (β isoform) (weak)
APPLICATION:
Western Blot (1:5’000)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles.
General Information
The regulatory A subunit (PR65) is tightly associated with catalytic subunit (PP2Ac), forming a scaffold to which the appropriate B subunit can bind. Different B subunits interact via the same or overlapping sites within the A subunit of the core dimer, which explains why binding of the B subunits is mutually exclusive. Two distinct PR65 isoforms (α and β) which share 86% sequence identity are ubiquitiously expressed. Recent studies reported that the genes encoding both the α and β isoforms of PR65/A were altered at low frequency in a variety of primary human tumors, identifying these subunits as tumorsuppressors. Partial or total loss (by cleavage through caspase-3) of the PR65/A subunit resulted in an increase in PP2A activity towards specific pro-apoptotic substrates involved in the cellular apoptotic response.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-569
Revised 24-Oct-06
Monoclonal Antibody to PP2A (Subunit B) (PR55) (α Isoform) (2G9)
SYNONYMS
anti-Protein Phosphatase 2A (Subunit B) (PR55) (α Isoform) MAb (2G9)
anti-PP2A B/PR55α MAb (2G9)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PP2A / Related Products
Ordering Information
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ALX-804-569-C100
100 µg
330.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE:
2G9
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 398-411 (C
398
ASGKRKKDEISVD
411
) of rat PP2A (subunit B) (PR55) (α isoform). This sequence is completely conserved in human, mouse, rat, pig, rabbit, chicken and xenopus.
SPECIFICITY:
Recognizes human, mouse and rat PP2A (subunit B) (PR55) (α isoform). Cross-reactivity with other species has not been tested. Detects a band of ~50kDa by Western blot. Cross-reacts with mouse and rat PP2A (subunit B) (PR55) (δ isoform)
.
CROSSREACTIVITY PROTEINS:
PP2A (subunit B) (PR55) (δ isoform)
APPLICATION:
Western Blot (1:1'000-1:10'000)
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Protein phosphatase 2A associates with and regulates atypical PKC and the epithelial tight junction complex
:
V. Nunbhakdi-Craig, et al.; J. Cell Biol.
158
, 967 (2002)
Abstract
;
Full Text
Identification of a subunit of a novel Kleisin-beta/SMC complex as a potential substrate of protein phosphatase 2A
:
F.M. Yeong, et al.; Curr. Biol.
13
, 2058 (2003)
Abstract
General Literature References
Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling:
V. Janssens and J. Goris; Biochem J.
353
, 417 (2001)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-210-917
Revised 02-Nov-06
Polyclonal Antibody to SET (human)
SYNONYMS
anti-PHAPII (human) PAb
anti-I
2
α
PP2A
(human) PAb
anti-Phosphatase 2A Inhibitor Protein 2α (human) PAb
anti-HLA-DR-associated Protein 2 (human) PAb
anti-IGAAD (human) PAb
anti-Inhibitor of Granzyme-activated DNase (human) PAb
anti-TAF-Iβ (human) PAb
anti-Template-activating Factor Iβ (human) PAb
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PP2A / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-210-917-C050
50 µg
194.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml
PURITY DETAIL:
Protein A affinity-purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 3-16 (A
3
PAAKVSKKELNSN
16
) of human SETβ.
SPECIFICITY:
Recognizes human SETβ. Detects endogenous protein by IP and WB.
APPLICATION:
Flow cytometry (1:100-1:200);
Immunocytochemistry;
Immunoprecipitation;
Western blot (1:1'000).
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for at least one year after receipt if stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Granzyme A activates an endoplasmic reticulum-associated caspase-independent nuclease to induce single-stranded DNA nicks
:
P.J. Beresford, et al.; J. Biol. Chem.
276
, 43285 (2001)
Abstract
;
Full Text
HMG2 interacts with the nucleosome assembly protein SET and is a target of the cytotoxic T-lymphocyte protease granzyme A
:
Z. Fan, et al.; Mol. Cell. Biol.
22
, 2810 (2002)
Abstract
;
Full Text
Cleaving the oxidative repair protein Ape1 enhances cell death mediated by granzyme A
:
Z. Fan, et al.; Nat. Immunol.
4
, 145 (2003)
Abstract
Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor
:
Z. Fan, et al.; Cell
112
, 659 (2003)
Abstract
Granzyme a induces caspase-independent mitochondrial damage, a required first step for apoptosis:
D. Martinvalet, et al.; Immunity
22
, 355 (2005)
Abstract
General Information
MANUFACTURER
Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Granzymes Other Products
•
Polyclonal Antibodies
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