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ALX-350-289
Revised 18-Nov-08
10Z-Hymenialdisine
SYNONYMS
4-(2-Amino-4-oxo-2-imidazolin-5-ylidene)-2-bromo-4,5,6,7-tetrahydropyrrolo[2,3-
c
]azepin-8-one
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Natural Products - Protein Kinase Inhibitors
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-350-289-C500
500 µg
190.00 USD
ALX-350-289-M001
1 mg
340.00 USD
Product Specification
FORMULA:
C
11
H
10
BrN
5
O
2
MW:
324.1
CAS NUMBER:
82005-12-7
SOURCE/HOST:
Isolated from sponge
Axinella carteri
.
PURITY:
≥97% (HPLC)
APPEARANCE:
Yellow needles.
SOLUBILITY:
Soluble in DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
HAZARD:
TOXIC.
IDENTITY:
Identity determined by
1
H- NMR and MS.
Product Description
Originally isolated from the sponges
Axinella verrucosa
and
Acantella aurantiaca
. Potent inhibitor of mitogen-activated protein kinase kinase-1 (MEK-1) (IC
50
=6nM). Blocks the
in vivo
phosphorylation of the microtubule-binding protein tau at sites that are hyperphosphorylated by glycogen synthase kinase-β (GSK-3β) and CDK5/p35 in Alzheimer’s disease. Inhibitor of DNA damage checkpoint at G2 phase (IC
50
=6µM), cyclin-dependent kinases CDK1/cyclin B (IC
50
=22nM), CDK2/cyclin A (IC
50
=70nM), CDK2/cyclin E (IC
50
=40nM), CDK4/cyclin D1 (IC
50
=600nM), CDK5/p25 (IC
50
=28nM), GSK-3β
(IC
50
=10nM), and
casein kinase 1 (CK1) (IC
50
=35nM). Inhibitor of NF-κB activation and of various pro-inflammatory cytokines such as IL-1, IL-2 (IC
50
=2.4µM), IL-6, IL-8, TNF-α (IC
50
=1.4µM) and nitric oxide (NO) (IC
50
=0.8µM) in a variety of cell lines.
Product Specific Literature References
Isolation and x-ray crystal structure of a novel bromo-compound from two marine sponges
:
G. Cimino, et al.; Tetrahedron Lett.
23
, 767 (1982)
Bioactive alkaloids from the tropical marine sponge Axinella carteri
:
A. Supriyono, et al.; Z. Naturforsch. [C]
50
, 669 (1995)
Abstract
The natural product hymenialdisine inhibits interleukin-8 production in U937 cells by inhibition of nuclear factor-kappaB
:
J.J. Breton & M.C. Chabot-Fletcher; J. Pharmacol. Exp. Ther.
282
, 459 (1997)
Abstract
;
Full Text
Inhibition of NFkappaB-mediated interleukin-1beta-stimulated prostaglandin E2 formation by the marine natural product hymenialdisine
:
A. Roshak, et al.; J. Pharmacol. Exp. Ther.
283
, 955 (1997)
Abstract
;
Full Text
Inhibition of interleukin-1-induced proteoglycan degradation and nitric oxide production in bovine articular cartilage/chondrocyte cultures by the natural product, hymenialdisine
:
A.M. Badger, et al.; J. Pharmacol. Exp. Ther.
290
, 587 (1999)
Abstract
;
Full Text
Inhibition of cyclin-dependent kinases, GSK-3beta and CK1 by hymenialdisine, a marine sponge constituent
:
L. Meijer, et al.; Chem. Biol.
7
, 51 (2000)
Abstract
Inhibition of the G2 DNA damage checkpoint and of protein kinases Chk1 and Chk2 by the marine sponge alkaloid debromohymenialdisine
:
D. Curman, et al.; J. Biol. Chem.
276
, 17914 (2001)
Abstract
;
Full Text
An investigation of cell proliferation and soluble mediators induced by interleukin 1beta in human synovial fibroblasts: comparative response in osteoarthritis and rheumatoid arthritis
:
H. Inoue, et al.; Inflamm. Res.
50
, 65 (2001)
Abstract
Aldisine alkaloids from the Philippine sponge Stylissa massa are potent inhibitors of mitogen-activated protein kinase kinase-1 (MEK-1)
:
D. Tasdemir, et al.; J. Med. Chem.
45
, 529 (2002)
Abstract
Inhibition of cytokine production by hymenialdisine derivatives
:
V. Sharma, et al.; J. Med. Chem.
47
, 3700 (2004)
Abstract
Further Categories Containing This Product:
CDK & Cyclin Inhibitors
•
MAPK Pathway Inhibitors
•
Natural Products - NF-kB Pathway Inhibitors
•
NF-kB Pathway Inhibitors
•
Natural Products for Cell Cycle Research
•
Alkaloids
•
Natural Products - Nitric Oxide Pathway Modulators
•
DNA Damage Checkpoint Other Products
•
GSK-3 Inhibitors
•
Marine Natural Products
ALX-804-229
Revised 11-Jan-07
Monoclonal Antibody to Rad9 (human) (93A535)
PRODUCT LINE
Cell Cycle
PRODUCT CATEGORY
DNA Damage Checkpoint Other Products
Ordering Information
Product Numbers:
Format:
Size:
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Quantity:
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ALX-804-229-C100
100 µg
293.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
93A535
ISOTYPE:
Mouse IgG1
CONCENTRATION:
0.5mg/ml
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN:
Recombinant human Rad9.
SPECIFICITY:
Recognizes human Rad9. Detects a band of ~50kDa by Western blot.
APPLICATION:
Immunocytochemistry (see [7])
Immunoprecipitation
Western Blot (1-2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 6 months when stored at +4°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
[1]
Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage:
R.H. Schiestl, et al.; Mol. Cell. Biol.
9
, 1882 (1989)
Abstract
[2]
Characterization of RAD9 of Saccharomyces cerevisiae and evidence that its function acts posttranslationally in cell cycle arrest after DNA damage:
T.A. Weinert & L.H. Hartwell; Mol. Cell. Biol.
10
, 6554 (1990)
Abstract
[3]
A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene:
H.B. Lieberman, et al.; PNAS
93
, 13890 (1996)
Abstract
[4]
MEC1-dependent phosphorylation of Rad9p in response to DNA damage:
A. Emili; Mol. Cell
2
, 183 (1998)
Abstract
[5]
Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex:
E. Volkmer & L.M. Karnitz; J. Biol. Chem.
274
, 567 (1999)
Abstract
;
Full Text
[6]
The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1:
R.P. St. Onge, et al.; Mol. Biol. Cell
10
, 1985 (1999)
Abstract
[7]
Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-x L and promotes apoptosis:
K. Komatsu, et al.; Nature Cell Biol.
2
, 1 (2000)
Abstract
General Information
Rad9 is a cell cycle checkpoint protein: DNA damage leads to extensive phosphorylation of Rad9 [4] which then inhibits progression from G2 in order to preserve cell viability, allowing proper ligation of DNA breaks before the entry of cells into mitosis [1,2]. The human homolog [3] of S. pombe Rad9 is physically proximal to the upstream (DNA damage) signal and associates with two other checkpoint control proteins, Rad1 and HHUS1 [5,6]. Rad9 also interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Overexpression of Rad9 in mammalian cells leads to apoptosis, where as antisense Rad9 RNA suppresses cell death induced by methyl methanesulphonate [7].
Further Categories Containing This Product:
DNA Replication Other Products
•
Monoclonal Antibodies
ALX-210-419
Revised 30-Oct-06
Polyclonal Antibody to 53BP1
SYNONYMS
anti-Tumor Protein p53 Binding Protein 1 PAb
anti-TP53BP1 PAb
anti-p202 PAb
PRODUCT LINE
Cell Cycle
PRODUCT CATEGORY
p53 Other Products
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Size:
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ALX-210-419-R050
50 µl
180.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant human 53BP1 (tumor protein p53 binding protein 1) (aa 1308-1824).
SPECIFICITY:
Recognizes native and denatured forms of full-length human 53BP1. Cross-reacts with mouse 53BP1.
APPLICATION:
Immunocytochemistry (1:200);
Immunoprecipitation;
Western blot (1:2'000).
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Storage at +4°C is recommended; if freezing is prefered add 50% glycerol.
Product Images
Please click on thumbnails to enlarge.
Product Description
The 53BP1 protein is implicated in the early response to DNA damage. The residues aa 1308-1824 are central to the antigen sequence recognized by the antibody
ALX-210-419
.
Product Specific Literature References
Phosphorylation and rapid relocalization of 53BP1 to nuclear foci upon DNA damage
:
L. Anderson, et al.; Mol. Cell. Biol.
21
, 1719 (2001)
Abstract
Kinetochore localisation of the DNA damage response component 53BP1 during mitosis
:
D. Jullien, et al.; J. Cell Sci.
115
, 71 (2002)
Abstract
;
Full Text
Further Categories Containing This Product:
DNA Damage Checkpoint Other Products
•
Polyclonal Antibodies
ALX-201-233
Revised 22-Jun-07
Rad9/Rad1/Hus1 (human) (recombinant)
SYNONYMS
9-1-1 Complex (human) (recombinant)
PRODUCT LINE
Cell Cycle
PRODUCT CATEGORY
DNA Damage Checkpoint Other Products
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Product Numbers:
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Size:
Unit Price:
Quantity:
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ALX-201-233-C005
5 µg
240.00 USD
Product Specification
MW:
106kDa (heterotrimer of 43, 31 and 32kDa)
SOURCE/HOST:
Produced in baculovirus.
CONCENTRATION:
1mg/ml
PURITY:
≥90% (by SDS-PAGE).
FORMULATION:
Liquid. In 25mM Tris, pH 8.0, containing 20% glycerol, 100mM sodium chloride and 1mM β-mercaptoethanol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair:
M. Toueille, et al.; Nucl. Acids Res.
32
, 3316 (2004)
Abstract
;
Full Text
The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery:
E. Smirnova, et al.; Biochem. J.
389
, 13 (2005)
Abstract
General Literature References
Regulation of the DNA replication fork: a way to fight genomic instability:
M. Toueille and U. Hubscher; Chromosoma
113
, 113 (2004)
Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
DNA Replication Other Products
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