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ALX-210-137/1
Revised 21-Aug-07
Polyclonal Antibody to BiP
SYNONYMS
anti-GRP78 PAb
anti-Glucose Regulated Protein 78 PAb
PRODUCT LINE
Stress & Heat Shock Proteins
PRODUCT CATEGORY
HSP70 / DnaK / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-137/1-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
Others
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Lyophilized. Contains 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with PBS.
IMMUNOGEN:
Synthetic peptide corresponding to aa 643-654 (T
643
GEEDTSEKDEL
654
) of rat BiP (GRP78; glucose regulated protein 78kDa).
SPECIFICITY:
Recognizes mouse and rat BiP. Does not detect BiP from human cell lines or COS cells.
APPLICATION:
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress
•
Polyclonal Antibodies
•
Autophagy Other Products
ALX-210-312
Revised 26-Mar-08
Polyclonal Antibody to Bone Sialoprotein
PRODUCT LINE
Bone Metabolism
PRODUCT CATEGORY
Matrix Proteins Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-312-R020
20 µl
135.00 USD
ALX-210-312-R100
100 µl
660.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml after reconstitution.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute with 20μl or 100μl distilled water.
IMMUNOGEN:
Purified human bone sialoprotein (bone extract).
SPECIFICITY:
Recognizes human and rat bone sialoprotein. Does not cross-react with human osteopontin or osteonectin.
APPLICATION:
ELISA
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections)
Western Blot
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-311
Revised 30-May-07
Polyclonal Antibody to Bone Sialoprotein (human)
PRODUCT LINE
Bone Metabolism
PRODUCT CATEGORY
Matrix Proteins Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-210-311-C010
10 µg
130.00 USD
ALX-210-311-C100
100 µg
577.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From chicken.
CONCENTRATION:
1mg/ml after reconstitution.
FORMULATION:
Lyophilized. Contains PBS, pH 7.4.
RECONSTITUTION:
Reconstitute with distilled water.
IMMUNOGEN:
Purified human bone sialoprotein (bone extract).
SPECIFICITY:
Recognizes human bone sialoprotein. Does not cross-react with human osteopontin, osteonectin or bone alkaline phosphatase.
APPLICATION:
ELISA (1µg/ml)
Flow Cytometry
Immunocytochemistry (1:100)
Immunohistochemistry (paraffin sections (2ng/ml))
Western Blot (2ng/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
Improved purification of human bone sialoprotein and development of a homologous radioimmunoassay:
M. Karmatschek, et al.; Clin. Chem.
43
, 2076 (1997)
Abstract
;
Full Text
Bone sialoprotein mRNA and protein expression in human multiple myeloma cell lines and patients:
A. Bellahcene, et al.; Br. J. Haematol.
111
, 1118 (2000)
Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-313
Revised 14-Nov-06
Polyclonal Antibody to Bone Sialoprotein (human)
PRODUCT LINE
Bone Metabolism
PRODUCT CATEGORY
Matrix Proteins Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-313-R020
20 µl
135.00 USD
ALX-210-313-R100
100 µl
660.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
FORMULATION:
Lyophilized.
RECONSTITUTION:
Reconstitute with 20µl/100µl distilled water.
IMMUNOGEN:
Synthetic peptide corresponding to aa 262-279 (Y
262
ESENGEPRGDNYRAYED
279
) of human bone sialoprotein.
SPECIFICITY:
Recognizes human bone sialoprotein. Does not cross-react with human osteonectin or osteopontin.
APPLICATION:
ELISA (1:1'000)
Immunocytochemistry (1:100)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-872
Revised 11-Nov-05
Polyclonal Antibody to Bradykinin B2 Receptor
SYNONYMS
anti-B
2
R PAb
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Bradykinin Receptor / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-210-872-R200
200 µl
195.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Undiluted serum containing 0.01% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 356-391 of human B
2
R (bradykinin B
2
receptor).
SPECIFICITY:
Recognizes human, mouse and rat B
2
R.
APPLICATION:
Immunoprecipitation (use 2-5µl of undiluted antiserum per precipitate)
Western Blot (1:100-1:500)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Ligand-induced phosphorylation/dephosphorylation of the endogenous bradykinin B2 receptor from human fibroblasts
:
A. Blaukat, et al.; J. Biol. Chem.
271
, 32366 (1996)
Abstract
;
Full Text
Overexpression and functional characterization of kinin receptors reveal subtype-specific phosphorylation
:
A. Blaukat, et al.; Biochemistry
38
, 1300 (1999)
Abstract
Bradykinin-induced internalization of the human B2 receptor requires phosphorylation of three serine and two threonine residues at its carboxyl tail
:
A. Pizard, et al.; J. Biol. Chem.
274
, 13738 (1999)
Abstract
;
Full Text
Determination of bradykinin B2 receptor in vivo phosphorylation sites and their role in receptor function
:
A. Blaukat, et al.; J. Biol. Chem.
276
, 40431 (2001)
Abstract
;
Full Text
Functional specialization of calreticulin domains
:
K. Nakamura, et al.; J. Cell Biol.
154
, 961 (2001)
Abstract
Further Categories Containing This Product:
NOS Regulation
•
Cell Stress, Oxidative Stress & Redox Signalling Other Products
•
Polyclonal Antibodies
ALX-210-618
Revised 28-Aug-08
Polyclonal Antibody to N-cadherin
SYNONYMS
anti-CD325 PAb
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-210-618-C400
400 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
2mg/ml after reconstitution.
PURITY DETAIL:
Protein A-affinity purified.
FORMULATION:
Lyophilized from 10mM PBS, pH 7.4, containing 1% BSA and 0.1% sodium azide.
RECONSTITUTION:
Reconstitute with 200µl distilled water. Dilute with 20mM TBS, pH 7.4, containing 1% BSA and 0.1% sodium azide and 10mM calcium chloride.
IMMUNOGEN:
Synthetic peptide corresponding to aa 808-827 of human N-cadherin.
SPECIFICITY:
Recognizes human, mouse, bovine and chicken N-cadherin. Does not cross-react with human E-or P-cadherin.
APPLICATION:
Immunohistochemistry (frozen and paraffin sections (5-10μg/ml))
Western Blot (5-10μg/ml)
Optimal conditions must be determined individually for each application.
Note: Does not inhibit N-cadherin dependent cell-cell contact.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 2 years after receipt when stored at +4°C. Reconstituted antibody is stable for 6 months when stored at +4°C and for 1 year when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
TBS should be used for dilution as PBS may cause precipitation. In case of immunohistochemistry on paraffin or frozen sections, Ca
2+
is not necessary.
Further Categories Containing This Product:
Polyclonal Antibodies
•
CD Antibodies
ALX-210-232
Revised 07-Oct-08
Polyclonal Antibody to VE-cadherin
PRODUCT LINE
Cytoskeleton
PRODUCT CATEGORY
Cadherin Antibodies
Ordering Information
Product Numbers:
Format:
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ALX-210-232-C100
Purified
100 µg
308.00 USD
ALX-210-232B-T100
Biotin
100 tests
355.00 USD
ALX-210-232F-T100
FITC
100 tests
355.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Affinity purified using recombinant VE-cadherin fragments.
FORMULATION:
Unconjugated PAb
: Liquid. 1mg/ml in PBS.
Biotin- and FITC-conjugates:
Liquid. In 1ml 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
IMMUNOGEN:
Recombinant human VE-cadherin (aa 1-258).
SPECIFICITY:
Recognizes human, mouse, rat and bovine VE-cadherin.
APPLICATION:
Confocal Microscopy
[1]
ELISA
Flow Cytometry
Immunocytochemistry
Immunohistochemistry:
Frozen sections (formalin or acetone-fixed sections) (1µg/ml); not recommended for paraffin sections.
Western Blot
[1] (see also protocol modifications)
Functional Application:
Interferes with VE-cadherin adhesion (reversible endothelium disruption) This effect mimics the transmigration of leukocytes or cancer cells through endothelium.
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
USE/STABILITY:
For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. Keep Biotin- and FITC-conjugated formats at +4°C. Protect from light.
Product Specific Literature References
[1]
Alteration of endothelial cell monolayer integrity triggers resynthesis of vascular endothelium cadherin:
D. Gulino, et al.; J. Biol. Chem.
273
, 29786 (1998)
Abstract
;
Full Text
VE-cadherin-p120 interaction is required for maintenance of endothelial barrier function:
S. Iyer, et al.; Am. J. Physiol. Lung Cell Mol. Physiol.
286
, L1143 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Protocol Modifications for Western blot:
Protocol described in [1]; To improve the detection of VE cadherin, the following modifications are recommended
:
Use PAb to VE-cadherin (Prod. No. ALX-210-232) diluted to 1µg/ml in PBS containing 0.01% Thimerosal (concentration must be adjusted according to the batch used). The solution can be used several times. Incubate overnight at room temperature under stirring. Rinsing: 6 x 5 minutes plus 10 minutes at room temperature.
Secondary antibody
: Biotinylated goat anti-rabbit antibody (VC-BA-1000) diluted at 1:5’000 in 1% gelatin. Avoid exposure to light. Incubation 1h 30 at room temperature under stirring. Rinse in PBS 0.05% Tween 20 for 50 minutes at room temperature under stirring.
Signal amplification using Strept ABComplex/ HRP (Dako)
: Follow the protocol according to Dako instructions. Dilute Strept ABComplex/HRP to 1:5’000. Avoid exposure to light. Incubate 1h at room temperature while stirring. Rinse in PBS 0.05% Tween 20, 50 minutes at room temperature while stirring. Rinse rapidly in PBS.
Detection using the Supersignal Kit Pierce
: Incubate the membrane 10 minutes with the solution under red light. Membrane is then packed in Plastic wrap and put in contact with the film. This method should allow for the detection of very small amounts of VE cadherin (less than 1ng/well).
Further Categories Containing This Product:
Angiogenesis Other Products
•
Polyclonal Antibodies
ALX-210-119
Revised 30-Oct-06
Polyclonal Antibody to Calcineurin B
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
PP2B [Calcineurin] / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-210-119-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Others
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Antiserum diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant mouse calcineurin B.
SPECIFICITY:
Recognizes human, mouse and bovine calcineurin B. Does not cross-react with calcineurin A.
APPLICATION:
Immunoprecipitation
Western Blot (1:2'000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-126
Revised 07-Aug-07
Polyclonal Antibody to Calreticulin
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calreticulin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-126-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Antiserum containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant human calreticulin.
SPECIFICITY:
Recognizes human, mouse, rat, rabbit and monkey calreticulin.
APPLICATION:
Immunohistochemistry (1:100)
Immunocytochemistry
Immunoprecipitation
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Redistribution of intracellular Ca2+ stores during phagocytosis in human neutrophils:
O. Stendahl, et al.; Science
265
, 1439 (1994)
Abstract
Cell surface calreticulin is a putative mannoside lectin which triggers mouse melanoma cell spreading:
T.K. White, et al.; J. Biol. Chem.
270
, 15926 (1995)
Abstract
;
Full Text
Calreticulin interacts with newly synthesized human immunodeficiency virus type 1 envelope glycoprotein, suggesting a chaperone function similar to that of calnexin:
A. Otteken & B. Moss; J. Biol. Chem.
271
, 97 (1996)
Abstract
;
Full Text
Further Categories Containing This Product:
ER-Golgi Intermediate Compartment [ERGIC] / Related Products
•
Endoplasmatic Reticulum Stress
•
Polyclonal Antibodies
ALX-210-171
Revised 03-Jun-05
Polyclonal Antibody to Calreticulin (CT)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calreticulin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-171-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST:
From chicken.
FORMULATION:
Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 399-414 (D
399
EKEEDEEESPGQAKD
414
) of mouse calreticulin.
SPECIFICITY:
Recognizes human, mouse, rat, rabbit, dog and hamster calreticulin. Detects a band of ~60kDa and and unidentified band of ~120kDa by Western blot.
APPLICATION:
Western Blot.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-153-016
.
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Related Products
ALX-153-016
Blocking Peptide for PAb to Calreticulin (CT) (Prod. No.
ALX-210-171
)
Further Categories Containing This Product:
Endoplasmatic Reticulum Stress
•
ER-Golgi Intermediate Compartment [ERGIC] / Related Products
•
Polyclonal Antibodies
ALX-210-170/1
Revised 19-May-08
Polyclonal Antibody to Calreticulin (NT)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Calreticulin / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-170/1-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST:
From chicken.
CONCENTRATION:
1.1mg/ml
PURITY DETAIL:
Epitope-affinity purified IgY.
FORMULATION:
Liquid. Contains 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 24-43 (K
24
EQFLDGDAWTNRWVESKHK
43
) of mouse calreticulin.
SPECIFICITY:
Recognizes human, mouse, rat, dog and hamster calreticulin. Detects a band of ~60-66kDa by Western blot.
APPLICATION:
Immunocytochemistry
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
BLOCKING PEPTIDE:
For Blocking Peptide See Prod. No.
ALX-153-017
.
Product Images
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