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p53 / Related Products
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ALX-201-221 Revised 02-Oct-06
Pin1 (human) (recombinant)
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SYNONYMS Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant)
PPIase Pin1 (human) (recombinant)
Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant)
PPIase NIMA-interacting 1 (human) (recombinant)
Rotamase Pin1 (human) (recombinant)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-201-221-C100   100 µg 325.00 USD Add To Cart
Product Specification
MW: ~18.2kDa
SOURCE/HOST: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Recombinant Proteins / Fusion Proteins • Antiviral Signalling / Related Products • p53 Other Products
 
 
ALX-201-426 Revised 24-Jul-08 New product
Sirtuin 1 (human) (recombinant) (His)
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SYNONYMS Sirt1 (human) (recombinant) (His)
SIR2-like Protein 1 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Sirtuins / Related Products
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ALX-201-426-C010   10 µg 300.00 USD Add To Cart
ALX-201-426-C050   50 µg 470.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. The mature peptide of human sirtuin 1 (aa 2-555) is fused at the N-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM sodium chloride.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q96EB6: SIRT1 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • NAD+ Metabolism / Related Products • p53 Other Products
 
 
ALX-201-430 Revised 12-Aug-08 New product
Sirtuin 2 (human) (recombinant) (His)
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SYNONYMS Sirt2 (human) (recombinant) (His)
SIR2-like Protein 2 (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Sirtuins / Related Products
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ALX-201-430-C010   10 µg 300.00 USD Add To Cart
ALX-201-430-C050   50 µg 470.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. The mature peptide of human sirtuin 2 (aa 2-389) is fused at the N-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2μm-filtered solution in 50mM TRIS-Cl, pH 8.2, containing 100mM sodium chloride.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q8IXJ6: SIRT2 (human)
MANUFACTURER Manufactured by AdipoGen, Inc.
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Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • NAD+ Metabolism / Related Products • p53 Other Products
 
 
ALX-210-419 Revised 30-Oct-06
Polyclonal Antibody to 53BP1
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SYNONYMS anti-Tumor Protein p53 Binding Protein 1 PAb
anti-TP53BP1 PAb
anti-p202 PAb
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-210-419-R050   50 µl 180.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human 53BP1 (tumor protein p53 binding protein 1) (aa 1308-1824).
SPECIFICITY: Recognizes native and denatured forms of full-length human 53BP1. Cross-reacts with mouse 53BP1.
APPLICATION: Immunocytochemistry (1:200);
Immunoprecipitation;
Western blot (1:2'000).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Storage at +4°C is recommended; if freezing is prefered add 50% glycerol.
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Product Description
The 53BP1 protein is implicated in the early response to DNA damage. The residues aa 1308-1824 are central to the antigen sequence recognized by the antibody ALX-210-419.
Product Specific Literature References
Phosphorylation and rapid relocalization of 53BP1 to nuclear foci upon DNA damage: L. Anderson, et al.; Mol. Cell. Biol. 21, 1719 (2001) Abstract
Kinetochore localisation of the DNA damage response component 53BP1 during mitosis: D. Jullien, et al.; J. Cell Sci. 115, 71 (2002) Abstract; Full Text
Further Categories Containing This Product:
DNA Damage Checkpoint Other Products • Polyclonal Antibodies
 
 
ALX-210-768 Revised 11-Apr-06
Polyclonal Antibody to p51A
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-210-768-C200   200 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 2mg/ml
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 424-446 (E424PRRETPKQSDVFFRHSKPPNRS446) of C-terminal human p51A.
SPECIFICITY: Recognizes human and mouse p51A. Detects a band of ~54kDa by Western blot. Does not cross-react with p51B.
APPLICATION: Immunocytochemistry (1:300)
Immunohistochemistry (paraffin sections)
Western Blot (1:1'000)
QUALITY CONTROL: Western blot on mouse thymus, mouse heart and human T cell line Jurkat.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
BLOCKING PEPTIDE:

For Blocking Peptide see Prod. No. ALX-165-040.
General Information
p51 is a member of the p53 tumor suppressor gene family, which is induced by DNA damage and/or stress stimuli, causing cells to undergo G1-arrest or apoptotic death. Unlike ubiquitous expression of p53, expression of p51 is restricted to a limited number of tissues. The p51 gene encodes two major splicing variants, p51A (448 aa protein with a molecular weight of 50.9kDa) and p51B (641 aa protein with a molecular weight of 71.9kDa), a human homolog of rat Ket. p51A and p51B differ in their carboxyl-terminal structure.
BACKGROUND/TECHNICAL INFORMATION Most of the p51A protein in Jurkat and K562 tumor cell lines is degraded.
General Literature References
Cloning and functional analysis of human p51, which structurally and functionally resembles p53: M. Osada, et al.; Nature Med. 4, 839 (1998) Abstract
Human p53-p51 (p53-related) fusion protein: a potent BAX transactivator: S. Ikawa, et al.; Jpn. J. Cancer Res. 90, 596 (1999) Abstract
p53 family genes: structural comparison, expression and mutation: S. Ikawa, et al.; Cell Death Differ. 6, 1154 (1999) Abstract
p53 is a transcriptional activator of the muscle-specific phosphoglycerate mutase gene and contributes in vivo to the control of its cardiac expression: P. Ruiz-Lozano, et al.; Cell Growth Differ. 10, 295 (1999) Abstract; Full Text
Failure of viral oncoproteins to target the p53-homologue p51A: J. Roth & M. Dobbelstein; J. Gen. Virol. 80, 3251 (1999) Abstract; Full Text
Mutational analysis of p51A/TAp63gamma, a p53 homolog, in non-small cell lung cancer and breast cancer: M. Sunahara, et al.; Oncogene 18, 3761 (1999) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-769 Revised 11-Apr-06
Polyclonal Antibody to p51B
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-210-769-C200   200 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 2mg/ml.
PURITY DETAIL: Epitope-affinity purified IgG.
FORMULATION: Liquid. In PBS containing 1mg/ml BSA and 0.1% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 623-641(N623FDMDARRNKQQRIKEEGE641) of C-terminal human p51B.
SPECIFICITY: Recognizes human and mouse p51B. Detects a band of ~75kDA by Western blot. Does not cross-react with p51A.
APPLICATION: Immunocytochemistry (1:300)
Immunohistochemistry (paraffin sections)
Western Blot (1:1'000)
QUALITY CONTROL: Western blot on mouse lung and human K562 cell line. As expected, some of the p51B is degraded in the tumor cell line K562.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: For maximum product recovery after thawing, centrifuge the vial before opening the cap. Avoid freeze/thaw cycles.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-165-041.
General Information
p51 is a member of the p53 tumor suppressor gene family, which is induced by DNA damage and/or stress stimuli, causing cells to undergo G1-arrest or apoptotic death. Unlike ubiquitous expression of p53, expression of p51 is restricted to a limited number of tissues. The p51 gene encodes two major splicing variants, p51A (448 aa protein with a molecular weight of 50.9kDa) and p51B (641 aa protein with a molecular weight of 71.9kDa), a human homolog of rat Ket. p51A and p51B differ in their carboxyl-terminal structure.
General Literature References
Cloning and functional analysis of human p51, which structurally and functionally resembles p53  : M. Osada, et al.; Nat. Med. 4, 839 (1998) Abstract
Human p53-p51 (p53-related) fusion protein: a potent BAX transactivator  : S. Ikawa, et al.; Jpn. J. Cancer Res. 90, 596 (1999) Abstract
p53 family genes: structural comparison, expression and mutation  : S. Ikawa, et al.; Cell Death Differ. 6, 1154 (1999) Abstract
Failure of viral oncoproteins to target the p53-homologue p51A: J. Roth & M. Dobbelstein; J. Gen. Virol. 80, 3251 (1999) Abstract; Full Text
p53 is a transcriptional activator of the muscle-specific phosphoglycerate mutase gene and contributes in vivo to the control of its cardiac expression  : P. Ruiz-Lozano, et al.; Cell Growth Differ. 10, 295 (1999) Abstract; Full Text
Mutational analysis of p51A/TAp63gamma, a p53 homolog, in non-small cell lung cancer and breast cancer: M. Sunahara, et al.; Oncogene 18, 3761 (1999) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-947 Revised 13-Oct-08
Polyclonal Antibody to TIGAR (AT122)
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SYNONYMS anti-Tp53-induced Glycolysis and Apoptosis Regulator PAb (AT122)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Glycobiology Other Products
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ALX-210-947-C100   100 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human TIGAR (aa 1-270) fused at the N-terminus to a tag.
SPECIFICITY: Recognizes human and mouse TIGAR.
APPLICATION: Western Blot (1:1’000)
Note: Does not work for immunoprecipitation.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information

The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a novel p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
TIGAR, a p53-inducible regulator of glycolysis and apoptosis: K. Bensaad, et al.; Cell 126, 107 (2006) Abstract; Full Text
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Further Categories Containing This Product:
Cell Stress, Oxidative Stress & Redox Signalling Other Products • Polyclonal Antibodies • Cell Death / Apoptosis / Autophagy Other Products • p53 Other Products
 
 
ALX-210-961 Revised 05-Aug-08 New product
Polyclonal Antibody to AROS (human) (AT135)
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SYNONYMS anti-Active Regulator of SIRT1 (human) PAb (AT135)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Sirtuins / Related Products
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ALX-210-961-C100   100 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human AROS (aa 2-136)
SPECIFICITY: Recognizes human AROS.
APPLICATION: Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
General Information
SIRT1 is a member of the silent information regulator 2 (Sir2) family. It is an NAD+-dependent deacetylase protein that functions in cell death / survival, senescence, and endocrine signaling. It is also involved in the increase of life span following caloric restriction [1]. Recently, the first direct activator of SIRT1 has been described: AROS (active regulator of SIRT1) [2]. This nuclear protein of 142 amino acids interacts with SIRT1 and enhances SIRT1-mediated deacetylation of p53.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Sirtuins: Sir2-related NAD-dependent protein deacetylases: B.J. North & E. Verdin; Genome Biol. 5, 224 (2004) Abstract; Full Text
[2] Active regulator of SIRT1 cooperates with SIRT1 and facilitates suppression of p53 activity: E.J. Kim, et al.; Mol. Cell 28, 277 (2007) Abstract
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Further Categories Containing This Product:
NAD+ Metabolism / Related Products • Polyclonal Antibodies • p53 Other Products
 
 
ALX-270-270 Revised 17-Mar-08
Pifithrin-α . hydrobromide
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SYNONYMS 1-(4-Methylphenyl)-2-(4,5,6,7-tetrahydro-2-imino-3(2H)-benzothiazolyl)ethanone . HBr
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-270-270-M005   5 mg 50.00 USD Add To Cart
ALX-270-270-M010   10 mg 85.00 USD Add To Cart
ALX-270-270-M025   25 mg 200.00 USD Add To Cart
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Product Specification
FORMULA: C16H18N2OS . HBr
MW: 286.4 . 80.9
CAS NUMBER: 63208-82-2
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Keep under inert gas.
IDENTITY: Determined by 1H-NMR.

Product Description
Chemical inhibitor of p53 that protects mice from the side effects of cancer therapy. Reversibly blocks p53-dependent transcriptional activation and apoptosis.
Product Specific Literature References
Cancer research. A new way to combat therapy side effects: D. Ferber; Science 285, 1651 (1999) Abstract
A chemical inhibitor of p53 that protects mice from the side effects of cancer therapy: P.G. Komarov, et al.; Science 285, 1733 (1999) Abstract
Suppression of p53: a new approach to overcome side effects of antitumor therapy: E.A. Komarova & A.V. Gudkov; Biochemistry (Mosc) 65, 41 (2000), (Review) Abstract; Full Text
Neurons are protected from excitotoxic death by p53 antisense oligonucleotides delivered in anionic liposomes: A. Lakkaraju, et al.; J. Biol. Chem. 276, 32000 (2001) Abstract; Full Text
Wild-type and mutated presenilins 2 trigger p53-dependent apoptosis and down-regulate presenilin 1 expression in HEK293 human cells and in murine neurons: C. Alves da Costa, et al.; PNAS 99, 4043 (2002) Abstract
Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio: D. Javelaud & F. Besancon; J. Biol. Chem. 277, 37949 (2002) Abstract; Full Text
Transcription factor AP-2alpha triggers apoptosis in cardiac myocytes: F.U. Muller, et al.; Cell Death Differ. 11, 485 (2004) Abstract
Pifithrin-alpha inhibits p53 signaling after interaction of the tumor suppressor protein with hsp90 and its nuclear translocation: P.J. Murphy, et al.; J. Biol. Chem. 279, 30195 (2004) Abstract
 
 
ALX-270-287 Revised 26-Jun-08
Pifithrin-α (cyclic) . hydrobromide
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SYNONYMS 2-(4-Methylphenyl)imidazo[2,1-b]-5,6,7,8-tetrahydrobenzothiazole . HBr
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-270-287-M010   10 mg 170.00 USD Add To Cart
Product Specification
FORMULA: C16H16N2S . HBr
MW: 268.4 . 80.9
CAS NUMBER: 60477-34-1
PURITY: ≥95% (HPLC)
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in DMSO (100mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light and moisture. Keep under inert gas.

Product Description
Stable analog of pifithrin-α (Prod. No. ALX-270-270), with biological properties similar to its parent compound, but with reduced cytotoxicity. Inhibitor of p53. Reversibly inhibits p53-dependent transactivation of p53-responsive genes and reversibly blocks p53-mediated apoptosis. Potent STAT6 transcriptional inhibitor.
Product Specific Literature References
Cancer research. A new way to combat therapy side effects: D. Ferber; Science 285, 1651 (1999) Abstract
A chemical inhibitor of p53 that protects mice from the side effects of cancer therapy: P.G. Komarov, et al.; Science 285, 1733 (1999) Abstract
Suppression of p53: a new approach to overcome side effects of antitumor therapy: E.A. Komarova, et al.; Biochemistry 65, 41 (2000) Abstract; Full Text
Pifithrin-alpha inhibits p53 signaling after interaction of the tumor suppressor protein with hsp90 and its nuclear translocation: P.J. Murphy, et al.; J Biol Chem 279, 30195 (2004) Abstract
Related Products
 
 
ALX-270-310 Revised 10-Jul-08
PRIMA-1
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SYNONYMS 2,2-bis(Hydroxymethyl)-3-quinuclidinone
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
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ALX-270-310-M001   1 mg 23.00 USD Add To Cart
ALX-270-310-M005