ALEXIS Biochemicals: Sirtuin 2 (human) (recombinant) (His)

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ALX-350-015

Revised 15-Sep-08

(S)-(+)-Camptothecin
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Topoisomerase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-015-M050		50 mg	40.00 USD
ALX-350-015-M250		250 mg	150.00 USD
ALX-350-015-G001		1 g	295.00 USD

Product Specification

FORMULA:	C₂₀H₁₆N₂O₄
MW:	348.4
CAS NUMBER:	7689-03-4
MERCK INDEX:	14: 1735
RTECS:	UQ0492000
SOURCE/HOST:	Isolated from Mappia foetida Miers (Nothapodytes foetida (Wt.) Sleumer).
PURITY:	≥98% (HPLC)
APPEARANCE:	Pale yellow powder.
SOLUBILITY:	Soluble in DMSO (10mg/ml), methanol (40mg/ml) or 0.1N sodium hydroxide (50mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from light and moisture. After reconstitution, prepare aliquots and store at -20°C.
HAZARD:	TOXIC.

Product Description

Potent antitumor agent. Inhibitor of DNA-topoisomerase I. Activates p53 resulting in upregulated expression of TRAIL-R2 (DR5) and Bak to overcome TRAIL resistance in Bax-deficient human colon carcinoma cells. Suppresses nitric oxide (NO) biosynthesis.

Product Specific Literature References

M.E. Wall, et al.; JACS 88, 3888 (1966)
The current status of camptothecin analogues as antitumor agents: W.J. Slichenmyer, et al.; J. Natl. Cancer Inst. 85, 271 (1993), (Review) Abstract
RNA synthesis inhibitors increase melatonin production in Y79 human retinoblastoma cells: J.L. Janavs, et al.; Mol. Brain Res. 23, 47 (1994) Abstract
Camptothecins: from bench research to hospital wards: M. Potmesil; Cancer Res. 54, 1431 (1994), (Review) Abstract
Forskolin and camptothecin induce a 30 kDa protein associated with melatonin production in Y79 human retinoblastoma cells: J.L. Janavs, et al.; J. Neurosci. 15, 298 (1995) Abstract
Differential stabilization of eukaryotic DNA topoisomerase I cleavable complexes by camptothecin derivatives: A. Tanizawa, et al.; Biochemistry 34, 7200 (1995) Abstract
The anti-cancer drug camptothecin inhibits elongation but stimulates initiation of RNA polymerase II transcription: M. Ljungman & P.C. Hanawalt; Carcinogenesis 17, 31 (1996) Abstract
The cell cycle effects of camptothecin: Z. Darzynkiewicz, et al.; Ann. N. Y. Acad. Sci. 803, 93 (1996) Abstract
Camptothecin-induced apoptosis in p53-null human leukemia HL60 cells and their isolated nuclei: effects of the protease inhibitors Z-VAD-fmk and dichloroisocoumarin suggest an involvement of both caspases and serine proteases: T. Shimizu & Y. Pommier; Leukemia 11, 1238 (1997) Abstract
Camptothecin and taxol: discovery to clinic: M.E. Wall; Med. Res. Rev. 18, 299 (1998), Review Abstract
Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages: W.F. Chiou, et al.; Life Sci. 69, 625 (2001) Abstract
Reversal of multidrug resistance-associated protein-mediated daunorubicin resistance by camptothecin: D. Chauvier, et al.; J. Pharm. Sci. 91, 1765 (2002) Abstract
Camptothecin and taxol: historic achievements in natural products research: N.H. Oberlies & D.J. Kroll; J. Nat. Prod. 67, 129 (2004), Review Abstract
Camptothecin and its analogues: a review on their chemotherapeutic potential: D. Sriram, et al.; Nat. Prod. Res. 19, 393 (2005), Review Abstract
The effects of camptothecin on RNA polymerase II transcription: roles of DNA topoisomerase I:: G. Capranico, et al.; Biochimie 89, 482 (2007) Abstract

Related Products

ALX-350-133

Topotecan . hydrochloride

Further Categories Containing This Product:

MDR Inhibitors • Antitumor Agents (Enzyme Inhibitors) • Natural Products - Antitumor Reagents • Natural Products - Nitric Oxide Pathway Modulators • p53 Other Products • Alkaloids

ALX-270-437

Revised 07-Oct-08

6-Chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Sirtuins / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-437-M001		1 mg	110.00 USD

Product Specification

FORMULA:	C₁₃H₁₃ClN₂O
MW:	248.7
CAS NUMBER:	49843-98-3
PURITY:	≥95%
APPEARANCE:	White to beige powder.
SOLUBILITY:	Soluble in DMSO (10mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months when stored at -20°C in the dark.
HANDLING:	Protect from light.
IDENTITY:	Identity determined by ¹H-NMR, ¹³C-NMR and MS.

Product Description

Potent cell permeable and metabolically stable specific inhibitor of hSIRT1 (IC₅₀=98nM in vivo / IC₅₀=38nM in vitro; compared to hSIRT2: IC₅₀=19µM and hSIRT3: IC₅₀=48µM) with no effect on human histone deacetylases (HDACs) class I and class II, nor NAD glycohydrolase (IC₅₀>100µg). Inhibits the deacetylation of p53 (IC₅₀=1µM).

Product Specific Literature References

Discovery of indoles as potent and selective inhibitors of the deacetylase SIRT1: A.D. Napper, et al.; J. Med. Chem. 48, 8045 (2005) Abstract
Small molecule activators of SIRT1 as therapeutics for the treatment of type 2 diabetes: J.C. Milne, et al.; Nature 450, 712 (2007) Abstract

Further Categories Containing This Product:

p53 Other Products

ALX-804-495

Revised 22-Dec-06

Monoclonal Antibody to Pin1 (8C10)
SYNONYMS	anti-Peptidyl-prolyl cis-trans Isomerase Pin1 MAb (8C10) anti-PPIase Pin1 (8C10) MAb anti-Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 MAb (8C10) anti-PPIase NIMA-interacting 1 (8C10) MAb anti-Rotamase Pin1 MAb (8C10)
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Alzheimer Research Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-495-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	8C10
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:	Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1.
SPECIFICITY:	Recognizes human, mouse and dog Pin1. Detects a band of ~16 kDa by Western blot.
APPLICATION:	Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:	Yes (Prod. No. ALX-840-030).

General Information

Pin1, a member of the parvulin family of peptidyl-prolyl-cis-trans-isomerases (PPIases), specifically isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins like Bcl-2, Cdc25C, Cdc27, p53 and Tau. Binding of Pin1 to p53 stimulates the DNA binding activity and transactivation function of p53. Pin1 also regulates the expression of cyclin D1 and is strikingly overexpressed in a subset of human tumors like breast cancer and oral squamous cell carcinoma.

Further Categories Containing This Product:

Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • p53 Other Products • Monoclonal Antibodies

ALX-803-321

Revised 21-Oct-08

Monoclonal Antibody to VRK1 (human) (1F6)
SYNONYMS	anti-Vaccinia-related Kinase 1 (human) (1F6) MAb anti-Serine/threonine Protein Kinase VRK1 (human) (1F6) MAb
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Serine / Threonine Kinases Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-803-321-C100		100 µg	270.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	1F6
ISOTYPE:	Mouse IgG1
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human VRK1 (vaccinia-related kinase 1).
SPECIFICITY:	Recognizes human VRK1. Does not cross-react with mouse or rat VRK1.
APPLICATION:	Immunohistochemistry (1:500) Immunoprecipitation Western Blot (1:1’000-1:10’000) Optimal conditions must be determined individually for each application.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

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Product Description

Vaccinia-related kinase 1 (VRK1) contributes to p53 stabilization by partly interfering with its MDM2-mediated degradation.

Product Specific Literature References

p53 Stabilization and accumulation induced by human vaccinia-related kinase 1: F.M. Vega, et al.; Mol. Cell. Biol. 24, 10366 (2004) Abstract
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma: C.R. Santos, et al.; Mol. Cancer Res. 4, 177 (2006) Abstract
Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations: A. Valbuena, et al.; Arch. Biochem. Biophys. 465, 219 (2007) Abstract
Human VRK1 Is an Early Response Gene and Its Loss Causes a Block in Cell Cycle Progression: A. Valbuena, et al.; PLOS ONE 3, 1642 (2008) Abstract
Proteomic identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (Vaccinia-Related Kinase) activities: M. Sanz-García; Mol. Cell. Proteomics Epub ahead of print, (2008) Abstract

Further Categories Containing This Product:

Other Kinases in Cell Cycle Regulation • p53 Other Products • Monoclonal Antibodies

ALX-804-872

Revised 13-Oct-08

Monoclonal Antibody to TIGAR (human) (Woody-1)
SYNONYMS	anti-TP53-induced Glycolysis and Apoptosis Regulator (human) MAb (Woody-1)
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Glycobiology Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-872-C100		100 µg	380.00 USD

Product Specification

CLONE:	Woody-1
ISOTYPE:	Mouse IgG2b
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human TIGAR (aa 1-270).
SPECIFICITY:	Recognizes human TIGAR.
APPLICATION:	Immunoprecipitation (1:200) Western Blot (1:1‘000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

General Information

The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a novel p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

TIGAR, a p53-inducible regulator of glycolysis and apoptosis: Bensaad, et al.; Cell 126, 107 (2006) Abstract; Full Text

Related Products

ALX-210-947

Polyclonal Antibody to TIGAR (AT122)

Further Categories Containing This Product:

Cell Stress, Oxidative Stress & Redox Signalling Other Products • Cell Death / Apoptosis / Autophagy Other Products • p53 Other Products • Monoclonal Antibodies

ALX-430-128

Revised 18-Nov-08

Nutlin-3
SYNONYMS	(±)-4-[4,5-bis-(4-Chlorophenyl)-2-(2-isopropoxy-4-methoxyphenyl)-4,5-dihydroimidazole-1-carbonyl]-piperazin-2-one
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-430-128-M001		1 mg	35.00 USD
ALX-430-128-M005		5 mg	160.00 USD
ALX-430-128-M025		25 mg	560.00 USD

Product Specification

FORMULA:	C₃₀H₃₀Cl₂N₄O₄
MW:	581.5
CAS NUMBER:	548472-68-0
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white crystalline solid.
SOLUBILITY:	Soluble in methanol, 100% ethanol, DMSO, dimethyl formamide or acetonitrile; sparingly soluble in a 1:10 solution of ethanol:PBS, pH 7.2.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least one year after receipt when stored at -20°C. We do not recommend storing the aqueous solution for more than one day.
HAZARD:	IRRITANT.

Product Description

Inhibitor of p53/MDM2 interaction (IC₅₀=0.09µM).

Product Specific Literature References

In vivo activation of the p53 pathway by small-molecule antagonists of MDM2: L.T. Vassilev, et al.; Science 303, 844 (2004) Abstract
Reactivation of the p53 pathway as a treatment modality for KSHV-induced lymphomas: G. Sarek, et al.; J. Clin. Invest. 117, 1019 (2007) Abstract; Full Text

General Literature References

Drug discovery and p53: D.P. Lane, et al.; Drug Discov. Today 8, 347 (2003) Abstract

Further Categories Containing This Product:

Ubiquitin Ligases [E3]

ALX-850-057

Revised 24-Feb-05

p53 (human) ELISA Kit
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-057-KI01		1 Kit	619.00 USD

Product Specification

SENSITIVITY:	0.5U/ml
QUANTITY:	96 wells (~80 tests).
APPLICATION:	For the quantitative determination of human p53 in cell culture supernatants, human serum, plasma and other biological fluids.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

General Information

MANUFACTURER

Manufactured by Bender MedSystems.

Further Categories Containing This Product:

ELISA & EIA Kits

ALX-270-270

Revised 17-Mar-08

Pifithrin-α . hydrobromide
SYNONYMS	1-(4-Methylphenyl)-2-(4,5,6,7-tetrahydro-2-imino-3(2H)-benzothiazolyl)ethanone . HBr
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-270-M005		5 mg	50.00 USD
ALX-270-270-M010		10 mg	85.00 USD
ALX-270-270-M025		25 mg	200.00 USD

Product Specification

FORMULA:	C₁₆H₁₈N₂OS . HBr
MW:	286.4 . 80.9
CAS NUMBER:	63208-82-2
PURITY:	≥98%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in DMSO or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Keep under inert gas.
IDENTITY:	Determined by ¹H-NMR.

Product Description

Chemical inhibitor of p53 that protects mice from the side effects of cancer therapy. Reversibly blocks p53-dependent transcriptional activation and apoptosis.

Product Specific Literature References

Cancer research. A new way to combat therapy side effects: D. Ferber; Science 285, 1651 (1999) Abstract
A chemical inhibitor of p53 that protects mice from the side effects of cancer therapy: P.G. Komarov, et al.; Science 285, 1733 (1999) Abstract
Suppression of p53: a new approach to overcome side effects of antitumor therapy: E.A. Komarova & A.V. Gudkov; Biochemistry (Mosc) 65, 41 (2000), (Review) Abstract; Full Text
Neurons are protected from excitotoxic death by p53 antisense oligonucleotides delivered in anionic liposomes: A. Lakkaraju, et al.; J. Biol. Chem. 276, 32000 (2001) Abstract; Full Text
Wild-type and mutated presenilins 2 trigger p53-dependent apoptosis and down-regulate presenilin 1 expression in HEK293 human cells and in murine neurons: C. Alves da Costa, et al.; PNAS 99, 4043 (2002) Abstract
Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio: D. Javelaud & F. Besancon; J. Biol. Chem. 277, 37949 (2002) Abstract; Full Text
Transcription factor AP-2alpha triggers apoptosis in cardiac myocytes: F.U. Muller, et al.; Cell Death Differ. 11, 485 (2004) Abstract
Pifithrin-alpha inhibits p53 signaling after interaction of the tumor suppressor protein with hsp90 and its nuclear translocation: P.J. Murphy, et al.; J. Biol. Chem. 279, 30195 (2004) Abstract

ALX-270-287

Revised 26-Jun-08

Pifithrin-α (cyclic) . hydrobromide
SYNONYMS	2-(4-Methylphenyl)imidazo[2,1-b]-5,6,7,8-tetrahydrobenzothiazole . HBr
PRODUCT LINE	Cell Cycle
PRODUCT CATEGORY	p53 Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-287-M010		10 mg	170.00 USD

Product Specification

FORMULA:	C₁₆H₁₆N₂S . HBr
MW:	268.4 . 80.9
CAS NUMBER:	60477-34-1
PURITY:	≥95% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in DMSO (100mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light and moisture. Keep under inert gas.

Product Description

Stable analog of pifithrin-α (Prod. No. ALX-270-270), with biological properties similar to its parent compound, but with reduced cytotoxicity. Inhibitor of p53. Reversibly inhibits p53-dependent transactivation of p53-responsive genes and reversibly blocks p53-mediated apoptosis. Potent STAT6 transcriptional inhibitor.

Product Specific Literature References