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ALX-215-026 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3β/ζ (phosphorylated) (pSer185)
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SYNONYMS anti-KCIP-1 (phosphorylated) (pSer185) PAb
anti-Protein Kinase C Inhibitor Protein 1 (phosphorylated) (pSer185) PAb
anti-Protein 1054 (phosphorylated) (pSer185) PAb
anti-14-3-3α/δ (phosphorylated) (pSer185) PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-026-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From sheep.
PURITY DETAIL: Affinity-purified.
FORMULATION: Liquid. Contains no preservatives.
IMMUNOGEN: Synthetic phosphorylated peptide corresponding to aa 181-190 (E181ILNpSPEKAC190) of 14-3-3β and aa 180-189 of 14-3-3ζ.
SPECIFICITY: Recognizes human, mouse, rat, sheep and bovine 14-3-3β and 14-3-3ζ.
APPLICATION: Western Blot (1:300)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies • Polyclonal Antibodies
 
 
ALX-215-022 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3ε (CT)
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SYNONYMS anti-KCIP-1 PAb (CT)
anti-Protein Kinase C Inhibitor Protein 1 PAb (CT)
anti-14-3-3E PAb (CT)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-022-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 239-255 (CG239EEQNKEALQDVEDENQ255) of 14-3-3ε.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3ε.
APPLICATION: ELISA
Immunohistochemistry
Western Blot (1:3000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993) Abstract; Full Text
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994) Abstract
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995) Abstract
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996) Abstract; Full Text
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999) Abstract
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002) Abstract
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002) Abstract
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003) Abstract
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-023 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3η
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SYNONYMS anti-KCIP-1 PAb
anti-Protein Kinase C Inhibitor Protein 1 PAb
anti-Protein AS1 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-023-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-11 (G1DREQLLQRAR11C) of 14-3-3η.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3η.
APPLICATION: Immunohistochemistry (1:3200)
Western Blot (1:3000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993) Abstract; Full Text
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994) Abstract
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995) Abstract
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996) Abstract; Full Text
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999) Abstract
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002) Abstract
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002) Abstract
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003) Abstract
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-024 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3γ
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SYNONYMS anti-KCIP-1 PAb
anti-Protein Kinase C Inhibitor Protein 1 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-024-R100   100 µl 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-10 (V1DREQLVQKA10C) of 14-3-3γ.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3γ.
APPLICATION: ELISA
Immunohistochemistry (1:800)
Western Blot (1:6’000-1:12’000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein: H. Martin, et al.; FEBS Lett. 331, 296 (1993) Abstract; Full Text
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies: H. Martin, et al.; J. Neurochem. 63, 2259 (1994) Abstract
Purification of 14-3-3 protein and analysis of isoforms in chicken brain: Y. Patel, et al.; Biochim. Biophys. Acta 1222, 405 (1994) Abstract
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins: D.H. Jones, et al.; FEBS Lett. 368, 55 (1995) Abstract; Full Text
Expression and structural analysis of 14-3-3 proteins: D.H. Jones, et al.; J. Mol. Biol. 245, 375 (1995) Abstract
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation: C.P. Wheeler-Jones, et al.; Biochem. J. 315, 41 (1996) Abstract; Full Text
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain: M. Matto-Yelin, et al.; Mol. Biol. Cell 8, 1889 (1997) Abstract; Full Text
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease: J. Wiltfang, et al.; J. Neurochem. 73, 2485 (1999) Abstract
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants: A. Aitken; Plant Mol. Biol. 50, 993 (2002) Abstract
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain: H.C. Baxter, et al.; Neuroscience 109, 5 (2002) Abstract
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1: H.K. Chen, et al.; Cell 113, 457 (2003) Abstract
Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein: P. Steinacker, et al.; Mol. Cell. Biol. 25, 1339 (2005) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-215-027 Revised 21-Jul-06
Polyclonal Antibody to 14-3-3σ
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SYNONYMS anti-KCIP-1 PAb
anti-Protein Kinase C Inhibitor Protein 1 PAb
anti-Stratifin PAb
anti-SFN PAb
anti-Epithelial Cell Marker Protein 1 PAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY 14-3-3 Proteins/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-215-027-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Sheep
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum. Contains no preservatives.
IMMUNOGEN: Synthetic peptide corresponding to aa 1-10 (M1ERASLIQKA10C) of 14-3-3σ.
SPECIFICITY: Recognizes human, mouse, rat, sheep, bovine and chicken 14-3-3σ.
APPLICATION: Western Blot (1:3000)
Optimal condition must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
A nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo: M.L. Henriksson, et al.; Eur. J. Biochem. 269, 4921 (2002) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-419 Revised 30-Oct-06
Polyclonal Antibody to 53BP1
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SYNONYMS anti-Tumor Protein p53 Binding Protein 1 PAb
anti-TP53BP1 PAb
anti-p202 PAb
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-419-R050   50 µl 180.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Neat serum containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human 53BP1 (tumor protein p53 binding protein 1) (aa 1308-1824).
SPECIFICITY: Recognizes native and denatured forms of full-length human 53BP1. Cross-reacts with mouse 53BP1.
APPLICATION: Immunocytochemistry (1:200);
Immunoprecipitation;
Western blot (1:2'000).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Storage at +4°C is recommended; if freezing is prefered add 50% glycerol.
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Product Description
The 53BP1 protein is implicated in the early response to DNA damage. The residues aa 1308-1824 are central to the antigen sequence recognized by the antibody ALX-210-419.
Product Specific Literature References
Phosphorylation and rapid relocalization of 53BP1 to nuclear foci upon DNA damage: L. Anderson, et al.; Mol. Cell. Biol. 21, 1719 (2001) Abstract
Kinetochore localisation of the DNA damage response component 53BP1 during mitosis: D. Jullien, et al.; J. Cell Sci. 115, 71 (2002) Abstract; Full Text
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-803 Revised 09-Feb-05
Polyclonal Antibody to Ataxia telangiectasia Mutated (human)
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SYNONYMS anti-ATM (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Ataxia telangiectasia Mutated/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-803-C100   100 µg 330.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.1mg/ml
FORMULATION: 100µg of purified antibody in 1.0ml of 0.05M sodium phosphate buffer containing 0.1% sodium azide and 0.2% gelatin.
IMMUNOGEN: Synthetic peptide corresponding to aa 819-844 of human ATM (ataxia-telangiectasia mutated).
SPECIFICITY: Recognizes human ATM.
APPLICATION: Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze.
Product Description
Ataxia telangiectasia is a recesive childhood disease by mutations in the ATM (AT-mutated) gene. ATM protein and Ataxia telangiectasia and Rad3-related protein (ATR) belongs to a family of protein kinases that share similarities at their C-terminal region with the catalytic domain of PI(3)K (phosphoinositide 3-kinase). ATM and ATR function in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATM and ATR directly phosphorylate the breast/ovarian cancer susceptibility gene BRCA1 following DNA damage and also regulate Chk1, which is required for the G2/M checkpoint. Like DNA-PK ATR phosphorylates p53 on serine 15. ATM has also been shown to phosphorylate murine double minute 2 (MDM2) and to interact with histone deacetylase. ATM, but not ATR is cleaved during apoptosis very similar to PARP.
Product Specific Literature References
Ataxia-telangiectasia and cellular responses to DNA damage: M.S. Meyn; Cancer Res. 55, 5991 (1995) Abstract
Defective G2 checkpoint function in cells from individuals with familial cancer syndromes: R.S. Paules, et al.; Cancer Res. 55, 1763 (1995) Abstract
A mammalian cell cycle checkpoint pathway utilizing p53 and GADD45 is defective in ataxia-telangiectasia: M.B. Kastan, et al.; Cell 71, 587 (1992) Abstract
Radiosensitivity in ataxia-telangiectasia: a new explanation: R.B. Painter & B.R. Young; PNAS 77, 7315 (1980) Abstract
The complete sequence of the coding region of the ATM gene reveals similarity to cell cycle regulators in different species: K. Savitsky, et al.; Hum. Mol. Genet. 4, 2025 (1995) Abstract
A single ataxia telangiectasia gene with a product similar to PI-3 kinase: K. Savitsky, et al.; Science 268, 1749 (1995) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Recommendations for Western blotting

ATM is a high MW protein of ~350kDa and as such, conditions may require optimization. 100-150µg of cell lysate is loaded on 5% acrylamide gels (until 200kDa marker is halfway through the gel) and transferred to nitrocellulose using semi-dry transfer at 9V constant voltage for 2 hours. Detection should be done using HRP-conjugated anti-rabbit IgG. This antibody may detect several smaller non-specific bands present in both ATM mutant and wild type cells. Therefore, careful titering of antibody concentrations is recommended.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-798 Revised 27-Dec-06
Polyclonal Antibody to ATR (human)
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SYNONYMS anti-Ataxia telangiectasia and Rad3-related Protein (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Ataxia telangiectasia Mutated/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-798-R100   100 µl 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 4mg/ml
FORMULATION: Liquid. Affinity purified IgG in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human ATR (ataxia telangiectasia and Rad3-related protein) (aa 400-460).
SPECIFICITY: Recognizes human ATR. Detects a band of ~305kDa by Western blot.
APPLICATION: Immunoprecipitation
Western Blot (1:1'000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-066 Revised 30-Oct-06
Polyclonal Antibody to ATRIP (human)
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SYNONYMS anti-ATR-interacting Protein (human) PAb
anti-ATM and Rad3-related Interacting Protein (human) PAb
anti-Ataxia telangiectasia and Rad3-related Interacting Protein (human) PAb
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Ataxia telangiectasia Mutated/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-066-C050   50 µg 220.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: ~3.8mg/ml
FORMULATION: Liquid. Protein G purified antibody in PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Recombinant human ATRIP (Ataxia telangiectasia and Rad3-related interacting protein) (aa 1-182).
SPECIFICITY: Recognizes human ATRIP.
APPLICATION: Immunohistochemistry
Immunoprecipitation (0.1-0.5µg)
Western Blot (0.75µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
ATR and ATRIP: partners in checkpoint signaling: D. Cortez, et al.; Science 294, 1713 (2001) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-210-705 Revised 13-Sep-05
Polyclonal Antibody to CDK2 (human)
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SYNONYMS anti-Cyclin-dependent Kinase 2 (human) PAb
anti-Cell Division Cycle Protein Kinase 2 (human) PAb
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-705-R100   100 µl 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
FORMULATION: Liquid. Contains 0.1% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to the C-terminal 12 aa of human CDK2 [9].
SPECIFICITY: Recognizes human CDK2.
APPLICATION: Immunoprecipitation (1:500)
Western Blot (1:500)
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -20°C
Product Specific Literature References
[1] Isolation of the human cdk2 gene