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ALX-804-126 Revised 04-May-07
Monoclonal Antibody to Clusterin (human) (CLI-9)
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SYNONYMS anti-TRPM-2 (human) MAb (CLI-9)
anti-Apolipoprotein J (human) MAb (CLI-9)
anti-APO-J (human) MAb (CLI-9)
anti-CLI (human) MAb (CLI-9)
anti-CLU (human) MAb (CLI-9)
anti-SGP-2 (human) MAb (CLI-9)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY Clusterin/Related Products
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ALX-804-126-C100   100 µg 280.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: CLI-9
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Purified human clusterin (TRPM-2).
SPECIFICITY: Recognizes human clusterin.
APPLICATION: Immunocytochemistry
Immunohistochemistry (frozen sections, paraffin sections)
Immunoprecipitation
Western Blot (1:2’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at +4°C undiluted.
HANDLING: Do not freeze/thaw.
Product Specific Literature References
Large-scale serial analysis of gene expression reveals genes differentially expressed in ovarian cancer  : C. D. Hough, et al.; Cancer Res. 60, 6281 (2000) Abstract
Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent: T. Chen, et al.; Cancer Res. 64, 7412 (2004) Abstract; Full Text
General Information
Clusterin (SGP-2/sulfated glycoprotein-2; CLI; Apolipoprotein J (Apo J); TRPM-2) is the first identified extracellular mammalian chaperone and appears to be implicated in such diverse processes as sperm maturation, regulation of complement activation, programmed cell death, tissue remodelling and lipid transport. Increases in clusterin mRNA and protein have been consistently detected in apoptotic cell death paradigms, establishing clusterin gene expression as a prominent marker of apoptotic cell loss. A dual role has been shown for clusterin in that cells can be protected from damage and dead cells can be more easily removed after some types of cellular damage.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Characterization and cloning of androgen-repressed mRNAs from rat ventral prostate: J.G. Leger, et al.; BBRC 147, 196 (1987) Abstract
Apolipoprotein J: structure and tissue distribution: H.V. De Silva, et al.; Biochemistry 29, 5380 (1990) Abstract
Clusterin: the intriguing guises of a widely expressed glycoprotein: D.E. Jenne & J. Tschopp; Trends Biochem. Sci. 17, 154 (1992) Abstract
Clusterin: S.E. Jones and C. Jomary; Int. J. Biochem. Cell Biol. 34, 427 (2002), (Review) Abstract
Clusterin/Apolipoprotein J in human aging and cancer: I. Trougakos and E. Gonos; Int. J. Biochem. Cell Biol. 34, 1430 (2002) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-001 Revised 22-Nov-07
Monoclonal Antibody to Cyclin D1 (DCS-6)
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SYNONYMS anti-Bcl-1 MAb (DCS-6)
anti-PRAD-1 MAb (DCS-6)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Antibodies
Ordering Information
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ALX-804-001-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: DCS-6
ISOTYPE: Mouse IgG2a
CONCENTRATION: 0.1mg/ml
FORMULATION: Liquid. In 0.05M sodium phosphate buffer containing 0.2% gelatin and 0.1% sodium azide.
IMMUNOGEN: Recombinant human cyclin D1.
SPECIFICITY: Recognizes human, mouse and rat cyclin D1. Does not cross-react with cyclins D2 or D3.
APPLICATION:

Flow Cytometry (5µg/ml)
Immunocytochemistry (5µg/ml)
Immunohistochemistry (frozen sections, paraffin sections (5µg/ml) - pretreatment with pepsin or trypsin is required)
Immunoprecipitation (see [3])
Western Blot (1µg/ml, see [3])

Functional Application: Neutralization studies (see [3])
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Do not freeze.
Product Specific Literature References
[1] Amplification and overexpression of cyclin D1 in breast cancer detected by immunohistochemical staining: C. Gillett, et al.; Cancer Res. 54, 1812 (1994) Abstract
[2] Cell cycle-related variation and tissue-restricted expression of human cyclin D1 protein: J. Bartkova, et al.; J. Pathol. 172, 237 (1994) Abstract
[3] Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines: J. Lukas, et al.; Oncogene 9, 707 (1994) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies • Tumor Markers
 
 
ALX-804-539 Revised 30-Nov-04
Monoclonal Antibody to Cyclin D1 (CD1.1)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Antibodies
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ALX-804-539-C100   100 µg 280.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
CLONE: CD1.1
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. In TBS containing carrier proteins and sodium azide.
IMMUNOGEN: Human cyclin D1.
SPECIFICITY: Recognizes human and rat cyclin D1.
APPLICATION: ELISA
Immunohistochemistry (paraffin sections (0.1-1µg/ml))
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Structure of Bcl-1 and IgH-CDR3 rearrangements as clonal markers in mantle cell lymphomas: C. Pott, et al.; Leukemia 12, 1630 (1998) Abstract
Classification of small B-cell lymphoid neoplasms using a paraffin section immunohistochemical panel: C.C. Chen, et al.; Appl. Immunohistochem. Mol. Morphol. 8, 1 (2000) Abstract
Multiparameter immunohistochemical analysis of the cell cycle proteins cyclin D1, Ki-67, p21WAF1, p27KIP1, and p53 in mantle cell lymphoma: K.F. Izban, et al.; Arch. Pathol. Lab. Med. 124, 1457 (2000) Abstract
Optimized cyclin D1 immunoperoxidase staining in mantle cell lymphoma: H.W. Korin, et al.; Appl. Immunohistochem. Mol. Morphol. 8, 57 (2000) Abstract
Disruption of the p16/cyclin D1/retinoblastoma protein pathway in the majority of human hepatocellular carcinomas: H. Azechi, et al.; Oncology 60, 346 (2001) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-046 Revised 25-Oct-04
Monoclonal Antibody to p16 (human) (DCS-50.1/H4)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDKs & Cyclins Other Products
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ALX-804-046-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: DCS-50.1/H4
ISOTYPE: Mouse IgG1
FORMULATION: Lyophilized. 100µg of purified antibody in 1ml sodium phosphate buffer containing 0.1% sodium azide and 0.2% gelatin.
IMMUNOGEN: Human p16 protein.
SPECIFICITY: Recognizes human p16.
APPLICATION: Immunohistochemistry (paraffin sections)
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-186 Revised 04-Sep-07
Monoclonal Antibody to p53 (phosphorylated) (pSer392) (9F4)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
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ALX-804-186-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 9F4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1 ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of p53 phosphorylated at Ser392.
SPECIFICITY: Recognizes activated human p53 phosphorylated at Ser392. Does not cross-react with the non-phosphorylated form of p53 or with unrelated serine-phosphorylated proteins.
APPLICATION: Western Blot: 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Further Categories Containing This Product:
Monoclonal Antibodies • Phospho-specific Antibodies • Tumor Markers
 
 
ALX-801-060 Revised 20-May-08
Monoclonal Antibody to p53 (human) (BP53-12)
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SYNONYMS anti-p53 (human) MAb (BP53-12)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
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ALX-801-060-C100   100 µg 210.00 USD Add To Cart
ALX-801-060F-C100 FITC 100 µg 315.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE: BP53-12
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant human wild-type p53 protein.
SPECIFICITY: Antibody BP53-12 detects a denaturation resistant epitope (aa 16-25) in the human p53 protein.
APPLICATION: BP53-12:
ELISA
Immunocytochemistry
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (1-2µg/ml)
BP53-12-FITC:
Flow Cytometry
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies: J. Bartek, et al.; J. Pathol. 169, 27 (1993) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-495 Revised 22-Dec-06
Monoclonal Antibody to Pin1 (8C10)
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SYNONYMS anti-Peptidyl-prolyl cis-trans Isomerase Pin1 MAb (8C10)
anti-PPIase Pin1 (8C10) MAb
anti-Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 MAb (8C10)
anti-PPIase NIMA-interacting 1 (8C10) MAb
anti-Rotamase Pin1 MAb (8C10)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-804-495-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 8C10
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1.
SPECIFICITY: Recognizes human, mouse and dog Pin1. Detects a band of ~16 kDa by Western blot.
APPLICATION: Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED: Yes (Prod. No. ALX-840-030).
General Information
Pin1, a member of the parvulin family of peptidyl-prolyl-cis-trans-isomerases (PPIases), specifically isomerizes only the phosphorylated  Ser/Thr-Pro bonds in certain proteins like Bcl-2, Cdc25C, Cdc27, p53 and Tau. Binding of Pin1 to p53 stimulates the DNA binding activity and transactivation function of p53. Pin1 also regulates the expression of cyclin D1 and is strikingly overexpressed in a subset of human tumors like breast cancer and oral squamous cell carcinoma.
Further Categories Containing This Product:
Monoclonal Antibodies • Stress & Heat Shock Proteins Other Products • p53 Other Products • Antiviral Signalling/Related Products
 
 
ALX-801-091 Revised 04-Apr-06
Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PC10)
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SYNONYMS anti-PCNA MAb (PC10)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Proliferating Cell Nuclear Antigen [PCNA]/Related Products
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ALX-801-091-L001   1 ml 480.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Goat
Sheep
Dog
Pig
Monkey
Others
CLONE: PC10
ISOTYPE: Mouse IgG2a
FORMULATION: Liquid. In 0.01M PBS containing 0.1% sodium azide and 1% BSA.
IMMUNOGEN: Rat PCNA (proliferating cell nuclear antigen).
SPECIFICITY: Recognizes PCNA from all vertebrate species, insects and S. pombe. Intense nuclear staining of proliferating cells in normal and tumor tissues.
APPLICATION: Immunohistochemistry (paraffin sections): Can be used for formalin-fixed, paraffin-embedded tissues. Pretreatment is not required. Recommended dilution is ≥1:40 for biotin based detection. For optimal staining, incubate 20-60 min. at room temperature.
Optimal conditions must be determined individually.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
Proliferating Cell Nuclear Antigen (PCNA) is associated with the DNA replication fork and its major function is described as a processivity factor for replicative DNA polymerases (polymerases δ and ε ) acting as a sliding clamp. It has a highly conserved amino acid sequence and has been identified in all eukaryotes and is being used as a proliferating marker. PCNA is also able to interact with multiple partners involved in Okazaki fragment joining, DNA repair, DNA methylation and chromatin assembly. In DNA repair PCNA has been reported not only for nucleotide excision repair (NER) (in concert with XPG and ERCC1), but also for base excision repair (BER) (binding to uracil-DNA glycosylase) and mismatch repair (MMR) (binding to mismatch repair enzymes MSH2 & MLH1). It is further associated with cell cycle control through cdk-cyclins during G1 to S phase.
Further Categories Containing This Product:
Monoclonal Antibodies • Tumor Markers
 
 
ALX-804-380 Revised 02-Oct-07
Monoclonal Antibody to Pyk2 (phosphorylated) (pTyr402) (14F6)
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SYNONYMS anti-CAKβ (phosphorylated) (pTyr402) MAb (14F6)
anti-Proline-rich Tyrosine Kinase 2 (phosphorylated) (pTyr402) MAb (14F6)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY Focal Adhesion Kinase [FAK]/Related Products
Ordering Information
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ALX-804-380-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 14F6
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of human Pyk2 phosphorylated at Tyr402.
SPECIFICITY: Recognizes human, mouse and rat Pyk2 phosphorylated at Tyr402.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies
 
 
ALX-804-229 Revised 11-Jan-07
Monoclonal Antibody to Rad9 (human) (93A535)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY DNA Damage Checkpoint Other Products
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ALX-804-229-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 93A535
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant human Rad9.
SPECIFICITY: Recognizes human Rad9. Detects a band of ~50kDa by Western blot.
APPLICATION: Immunocytochemistry (see [7])
Immunoprecipitation
Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Images
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Product Specific Literature References
[1] Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage: R.H. Schiestl, et al.; Mol. Cell. Biol. 9, 1882 (1989) Abstract
[2] Characterization of RAD9 of Saccharomyces cerevisiae and evidence that its function acts posttranslationally in cell cycle arrest after DNA damage: T.A. Weinert & L.H. Hartwell; Mol. Cell. Biol. 10, 6554 (1990) Abstract
[3] A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene: H.B. Lieberman, et al.; PNAS 93, 13890 (1996) Abstract
[4] MEC1-dependent phosphorylation of Rad9p in response to DNA damage: A. Emili; Mol. Cell 2, 183 (1998) Abstract
[5] Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex: E. Volkmer & L.M. Karnitz; J. Biol. Chem. 274, 567 (1999) Abstract; Full Text
[6] The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1: R.P. St. Onge, et al.; Mol. Biol. Cell 10, 1985 (1999) Abstract
[7] Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-x L and promotes apoptosis: K. Komatsu, et al.; Nature Cell Biol. 2, 1 (2000) Abstract
General Information
Rad9 is a cell cycle checkpoint protein: DNA damage leads to extensive phosphorylation of Rad9 [4] which then inhibits progression from G2 in order to preserve cell viability, allowing proper ligation of DNA breaks before the entry of cells into mitosis [1,2]. The human homolog [3] of S. pombe Rad9 is physically proximal to the upstream (DNA damage) signal and associates with two other checkpoint control proteins, Rad1 and HHUS1 [5,6]. Rad9 also interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Overexpression of Rad9 in mammalian cells leads to apoptosis, where as antisense Rad9 RNA suppresses cell death induced by methyl methanesulphonate [7].