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ALX-804-539 Revised 30-Nov-04
Monoclonal Antibody to Cyclin D1 (CD1.1)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDK & Cyclin Antibodies
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-539-C100   100 µg 280.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
CLONE: CD1.1
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. In TBS containing carrier proteins and sodium azide.
IMMUNOGEN: Human cyclin D1.
SPECIFICITY: Recognizes human and rat cyclin D1.
APPLICATION: ELISA
Immunohistochemistry (paraffin sections (0.1-1µg/ml))
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Structure of Bcl-1 and IgH-CDR3 rearrangements as clonal markers in mantle cell lymphomas: C. Pott, et al.; Leukemia 12, 1630 (1998) Abstract
Classification of small B-cell lymphoid neoplasms using a paraffin section immunohistochemical panel: C.C. Chen, et al.; Appl. Immunohistochem. Mol. Morphol. 8, 1 (2000) Abstract
Multiparameter immunohistochemical analysis of the cell cycle proteins cyclin D1, Ki-67, p21WAF1, p27KIP1, and p53 in mantle cell lymphoma: K.F. Izban, et al.; Arch. Pathol. Lab. Med. 124, 1457 (2000) Abstract
Optimized cyclin D1 immunoperoxidase staining in mantle cell lymphoma: H.W. Korin, et al.; Appl. Immunohistochem. Mol. Morphol. 8, 57 (2000) Abstract
Disruption of the p16/cyclin D1/retinoblastoma protein pathway in the majority of human hepatocellular carcinomas: H. Azechi, et al.; Oncology 60, 346 (2001) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-046 Revised 25-Oct-04
Monoclonal Antibody to p16 (human) (DCS-50.1/H4)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDKs & Cyclins Other Products
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ALX-804-046-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: DCS-50.1/H4
ISOTYPE: Mouse IgG1
FORMULATION: Lyophilized. 100µg of purified antibody in 1ml sodium phosphate buffer containing 0.1% sodium azide and 0.2% gelatin.
IMMUNOGEN: Human p16 protein.
SPECIFICITY: Recognizes human p16.
APPLICATION: Immunohistochemistry (paraffin sections)
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-645 Revised 05-Mar-07
Monoclonal Antibody to TRIP-Br1 (TB1-27/2)
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SYNONYMS anti-SERTA Domain-containing Protein 1 MAb (TB1-27/2)
anti-SEI-1 MAb (TB1-27/2)
anti-CDK4-binding Protein p34SEI1 MAb (TB1-27/2)
anti-Transcriptional Regulator Interacting with the PHD-bromodomain 1 MAb (TB1-27/2)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY CDKs & Cyclins Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-645-C050   50 µg 240.00 USD Add To Cart
ALX-804-645-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Pig
Monkey
CLONE: TB1-27/2
ISOTYPE: Mouse IgG2a.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 27-42 (D27PGHAAVAQAPPAVAS42) of human TRIP-Br1 [SEI-1].
SPECIFICITY: Recognizes human, mouse, pig and monkey TRIP-Br1.
APPLICATION: Immunohistochemistry (1:200)
Immunoprecipitation
Western Blot (1:1’000-1:5’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
Exploiting the TRIP-Br family of cell cycle regulatory proteins as chemotherapeutic drug targets in human cancer: Z.J. Zang, et al.; (ahead of print), (2007)
General Information
TRIP-Br1 [SEI-1] functions as physiological integrator of transcriptional regulatory signals at E2F-responsive promoters to ensure proper execution of E2F-dependent cell cycle progression. TRIP-Br1 was recently defined as an oncogene.
General Literature References
RBT1, a novel transcriptional co-activator, binds the second subunit of replication protein A: J.M. Cho, et al.; Nucl. Acids Res. 28, 3478 (2000) Abstract; Full Text
TRIP-Br: a novel family of PHD zinc finger- and bromodomain-interacting proteins that regulate the transcriptional activity of E2F-1/DP-1: S.I. Hsu, et al.; EMBO J. 20, 2273 (2001) Abstract; Full Text
The Drosophila gene taranis encodes a novel trithorax group member potentially linked to the cell cycle regulatory apparatus: S. Calgaro, et al.; Genetics 160, 547 (2002) Abstract; Full Text
Regulation of CREB-mediated transcription by association of CDK4 binding protein p34SEI-1 with CBP: T. Hirose, et al.; Int. J. Mol. Med. 11, 705 (2003) Abstract
TRIP-Br links E2F to novel functions in the regulation of cyclin E expression during cell cycle progression and in the maintenance of genomic stability: K.G. Sim, et al.; Cell Cycle 3, 1296 (2004) Abstract
The nuclear protein p34SEI-1 regulates the kinase activity of cyclin-dependent kinase 4 in a concentration-dependent manner: J. Li, et al.; Biochemistry 43, 4394 (2004) Abstract
Expression of a human SOCS protein, HSOCP-1, in peripheral blood eosinophils from patients with atopic dermatitis: K. Ogawa, et al.; Int. Arch. Allergy Immunol. 134 Suppl 1, 2 (2004) Abstract
Dissection of CDK4-binding and transactivation activities of p34(SEI-1) and comparison between functions of p34(SEI-1) and p16(INK4A): J. Li, et al.; Biochemistry 44, 13246 (2005) Abstract
SEI family of nuclear factors regulates p53-dependent transcriptional activation: R. Watanabe-Fukunaga, et al.; Genes Cells 10, 851 (2005) Abstract; Full Text
CDCA4 is an E2F transcription factor family-induced nuclear factor that regulates E2F-dependent transcriptional activation and cell proliferation: R. Hayashi, et al.; J. Biol. Chem. 281, 35633 (2006) Abstract; Full Text
Further Categories Containing This Product:
Tumor Suppressors / Related Products • Monoclonal Antibodies • Transcription Regulators Other Products
 
 
ALX-801-060 Revised 20-May-08
Monoclonal Antibody to p53 (human) (BP53-12)
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SYNONYMS anti-p53 (human) MAb (BP53-12)
PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
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ALX-801-060-C100   100 µg 210.00 USD Add To Cart
ALX-801-060F-C100 FITC 100 µg 315.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Monkey
CLONE: BP53-12
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS, pH 7.4, containing 15mM sodium azide.
IMMUNOGEN: Recombinant human wild-type p53 protein.
SPECIFICITY: Antibody BP53-12 detects a denaturation resistant epitope (aa 16-25) in the human p53 protein.
APPLICATION: BP53-12:
ELISA
Immunocytochemistry
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (1-2µg/ml)
BP53-12-FITC:
Flow Cytometry
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Specific Literature References
Immunohistochemical analysis of the p53 oncoprotein on paraffin sections using a series of novel monoclonal antibodies: J. Bartek, et al.; J. Pathol. 169, 27 (1993) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-186 Revised 04-Sep-07
Monoclonal Antibody to p53 (phosphorylated) (pSer392) (9F4)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY p53 Antibodies
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ALX-804-186-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 9F4
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.1% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1 ml distilled water (15 min, RT).
IMMUNOGEN: Synthetic peptide corresponding to a portion of p53 phosphorylated at Ser392.
SPECIFICITY: Recognizes activated human p53 phosphorylated at Ser392. Does not cross-react with the non-phosphorylated form of p53 or with unrelated serine-phosphorylated proteins.
APPLICATION: Western Blot: 1µg/ml for AP/BCIP/NBT (MTT) detection; 0.1µg/ml for HRPO/ECL detection. Do not use milk/casein for blocking and dilution! Replace by BSA!
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Further Categories Containing This Product:
Phospho-specific Antibodies • Monoclonal Antibodies • Tumor Markers
 
 
ALX-803-321 Revised 21-Oct-08
Monoclonal Antibody to VRK1 (human) (1F6)
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SYNONYMS anti-Vaccinia-related Kinase 1 (human) (1F6) MAb
anti-Serine/threonine Protein Kinase VRK1 (human) (1F6) MAb
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Serine / Threonine Kinases Other Products
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ALX-803-321-C100   100 µg 270.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 1F6
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human VRK1 (vaccinia-related kinase 1).
SPECIFICITY: Recognizes human VRK1. Does not cross-react with mouse or rat VRK1.
APPLICATION: Immunohistochemistry (1:500)
Immunoprecipitation
Western Blot (1:1’000-1:10’000)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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Product Description
Vaccinia-related kinase 1 (VRK1) contributes to p53 stabilization by partly interfering with its MDM2-mediated degradation.
Product Specific Literature References
p53 Stabilization and accumulation induced by human vaccinia-related kinase 1: F.M. Vega, et al.; Mol. Cell. Biol. 24, 10366 (2004) Abstract
VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma: C.R. Santos, et al.; Mol. Cancer Res. 4, 177 (2006) Abstract
Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations: A. Valbuena, et al.; Arch. Biochem. Biophys. 465, 219 (2007) Abstract
Human VRK1 Is an Early Response Gene and Its Loss Causes a Block in Cell Cycle Progression: A. Valbuena, et al.; PLOS ONE 3, 1642 (2008) Abstract
Proteomic identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (Vaccinia-Related Kinase) activities: M. Sanz-García; Mol. Cell. Proteomics Epub ahead of print, (2008) Abstract
Further Categories Containing This Product:
Other Kinases in Cell Cycle Regulation • p53 Other Products • Monoclonal Antibodies
 
 
ALX-804-495 Revised 22-Dec-06
Monoclonal Antibody to Pin1 (8C10)
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SYNONYMS anti-Peptidyl-prolyl cis-trans Isomerase Pin1 MAb (8C10)
anti-PPIase Pin1 (8C10) MAb
anti-Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 MAb (8C10)
anti-PPIase NIMA-interacting 1 (8C10) MAb
anti-Rotamase Pin1 MAb (8C10)
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Alzheimer Research Other Products
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ALX-804-495-C100   100 µg 453.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE: 8C10
ISOTYPE: Mouse IgG1
PURITY DETAIL: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION: Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION: Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN: Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1.
SPECIFICITY: Recognizes human, mouse and dog Pin1. Detects a band of ~16 kDa by Western blot.
APPLICATION: Western Blot (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING: After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED: Yes (Prod. No. ALX-840-030).
General Information
Pin1, a member of the parvulin family of peptidyl-prolyl-cis-trans-isomerases (PPIases), specifically isomerizes only the phosphorylated  Ser/Thr-Pro bonds in certain proteins like Bcl-2, Cdc25C, Cdc27, p53 and Tau. Binding of Pin1 to p53 stimulates the DNA binding activity and transactivation function of p53. Pin1 also regulates the expression of cyclin D1 and is strikingly overexpressed in a subset of human tumors like breast cancer and oral squamous cell carcinoma.
Further Categories Containing This Product:
Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • p53 Other Products • Monoclonal Antibodies
 
 
ALX-804-872 Revised 13-Oct-08 New product
Monoclonal Antibody to TIGAR (human) (Woody-1)
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SYNONYMS anti-TP53-induced Glycolysis and Apoptosis Regulator (human) MAb (Woody-1)
PRODUCT LINE Obesity & Adipokines
PRODUCT CATEGORY Glycobiology Other Products
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ALX-804-872-C100   100 µg 380.00 USD Add To Cart
Product Specification
CLONE: Woody-1
ISOTYPE: Mouse IgG2b
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human TIGAR (aa 1-270).
SPECIFICITY: Recognizes human TIGAR.
APPLICATION: Immunoprecipitation (1:200)
Western Blot (1:1‘000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
The p53 tumor-suppressor protein prevents cancer development through various mechanisms, including the induction of cell-cycle arrest, apoptosis, and the maintenance of genome stability. TIGAR (TP53-induced glycolysis and apoptosis regulator) has been identified as a novel p53-inducible gene. TIGAR expression decreased fructose-2,6-bisphosphate levels in cells, resulting in an inhibition of glycolysis. In addition, TIGAR expression lowered intracellular reactive oxygen species (ROS) levels and protected cells from p53-induced apoptosis. Bensaad et al., proposed that TIGAR modulates the apoptotic response to p53, thereby allowing cells to survive mild or transient stress.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
TIGAR, a p53-inducible regulator of glycolysis and apoptosis: Bensaad, et al.; Cell 126, 107 (2006) Abstract; Full Text
Related Products
Further Categories Containing This Product:
Cell Stress, Oxidative Stress & Redox Signalling Other Products • Cell Death / Apoptosis / Autophagy Other Products • p53 Other Products • Monoclonal Antibodies
 
 
ALX-804-229 Revised 11-Jan-07
Monoclonal Antibody to Rad9 (human) (93A535)
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PRODUCT LINE Cell Cycle
PRODUCT CATEGORY DNA Damage Checkpoint Other Products
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ALX-804-229-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 93A535
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant human Rad9.
SPECIFICITY: Recognizes human Rad9. Detects a band of ~50kDa by Western blot.
APPLICATION: Immunocytochemistry (see [7])
Immunoprecipitation
Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
Product Images
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Product Specific Literature References
[1] Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage: R.H. Schiestl, et al.; Mol. Cell. Biol. 9, 1882 (1989) Abstract
[2] Characterization of RAD9 of Saccharomyces cerevisiae and evidence that its function acts posttranslationally in cell cycle arrest after DNA damage: T.A. Weinert & L.H. Hartwell; Mol. Cell. Biol. 10, 6554 (1990) Abstract
[3] A human homolog of the Schizosaccharomyces pombe rad9+ checkpoint control gene: H.B. Lieberman, et al.; PNAS 93, 13890 (1996) Abstract
[4] MEC1-dependent phosphorylation of Rad9p in response to DNA damage: A. Emili; Mol. Cell 2, 183 (1998) Abstract
[5] Human homologs of Schizosaccharomyces pombe rad1, hus1, and rad9 form a DNA damage-responsive protein complex: E. Volkmer & L.M. Karnitz; J. Biol. Chem. 274, 567 (1999) Abstract; Full Text
[6] The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1: R.P. St. Onge, et al.; Mol. Biol. Cell 10, 1985 (1999) Abstract
[7] Human homologue of S. pombe Rad9 interacts with BCL-2/BCL-x L and promotes apoptosis: K. Komatsu, et al.; Nature Cell Biol. 2, 1 (2000) Abstract
General Information
Rad9 is a cell cycle checkpoint protein: DNA damage leads to extensive phosphorylation of Rad9 [4] which then inhibits progression from G2 in order to preserve cell viability, allowing proper ligation of DNA breaks before the entry of cells into mitosis [1,2]. The human homolog [3] of S. pombe Rad9 is physically proximal to the upstream (DNA damage) signal and associates with two other checkpoint control proteins, Rad1 and HHUS1 [5,6]. Rad9 also interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Overexpression of Rad9 in mammalian cells leads to apoptosis, where as antisense Rad9 RNA suppresses cell death induced by methyl methanesulphonate [7].
Further Categories Containing This Product:
Monoclonal Antibodies • DNA Replication Other Products
 
 
ALX-801-091 Revised 29-Sep-08
Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PC10)