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Cell Death / Apoptosis / Autophagy
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ALX-850-229 Revised 29-May-08
Caspase Fluorometric Substrate Set
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-229-KI01   1 Set 553.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Contains 125µl (1mM) each of the following substrates:
- Caspase-1 Substrate, Ac-YVAD-AFC
- Caspase-2 Substrate, Ac-VDVAD-AFC
- Caspase-3 Substrate, Ac-DEVD-AFC
- Caspase-5 Substrate, Ac-WEHD-AFC
- Caspase-6 Substrate, Ac-VEID-AFC
- Caspase-8 Substrate, Ac-IETD-AFC
- Caspase-9 Substrate, Ac-LEHD-AFC
FORMULATION: Liquid. Ready-to-use in DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Fluorometric substrates for assaying activities of members of caspase family proteases. All substrates are provided in liquid ready-to-use form.
General Information
BACKGROUND/TECHNICAL INFORMATION Assay Procedure:
1. Induce apoptosis in cells by desired method. Concurrently incubate a control culture without induction.
2. Count cells and pellet 1-5x106 cells or use 50-200µg cell lysates if protein concentration has been
    measured.
3. Resuspend cells in 50µl of chilled Cell Lysis Buffer.
4. Incubate cells on ice for 10 minutes.
5. Add 50µl of 2x Reaction Buffer containing 10mM DTT to each sample.
6. Add 5µl of the 1mM AFC conjugated substrates (50µM final conc.) into each tube individually and
    incubate at 37°C for 1-2 hours.
7. Read samples in a fluorometer equipped with a 400-nm excitation filter and a 505-nm emission filter.
    For a plate-reading set-up, transfer the samples to a 96-well plate. You may perform the entire assay
    directly in a 96 well plate.

    Fold-increase in caspase activity can be determined by comparing these results with the level of the
    uninduced control.

    Note: Background reading from cell lysates and buffers should be subtracted from the readings of both
    induced and the uninduced samples before calculating fold increase in caspase activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Caspase Substrates • Panels / Sets
 
 
ALX-850-275 Revised 29-May-08
Caspase Fluorometric Substrate Set PLUS
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-275-KI01   1 Set 735.00 USD Add To Cart
Product Specification
QUANTITY: 7x25 tests
KIT/SET CONTAINS: Contains the following components:
- Caspase-1 Substrate, Ac-YVAD-AFC (125µl)
- Caspase-2 Substrate, Ac-VDVAD-AFC (125µl)
- Caspase-3 Substrate, Ac-DEVD-AFC (125µl)
- Caspase-5 Substrate, Ac-WEHD-AFC (125µl)
- Caspase-6 Substrate, Ac-VEID-AFC (125µl)
- Caspase-8 Substrate, Ac-IETD-AFC (125µl)
- Caspase-9 Substrate, Ac-LEHD-AFC (125µl)
- Cell Lysis Buffer (100ml)
- 2x Reaction Buffer (20ml)
- DTT (1M) (400µl)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION

Assay Procedure:
1. Induce apoptosis in cells by desired method. Concurrently incubate a control culture without induction.
2. Count cells and pellet 1-5 x 106 cells or use 50-200 μg cell lysates if protein concentration has been 
    measured.
3. Resuspend cells in 50 μl of chilled Cell Lysis Buffer.
4. Incubate cells on ice for 10 minutes. 
5. Add 50 μl of 2x Reaction Buffer and 1 μl DTT to each sample.
6. Add 5 μl of the 1 mM AFC conjugated substrates (50 μM final conc.) into each tube individually and 
    incubate at 37°C for 1-2 hour.
7. Read samples in a fluorometer equipped with a 400 nm excitation filter and 505 nm emission filter. For
    a plate-reading set-up, transfer the samples to a 96-well plate. You may perform the entire assay
    directly in a 96-well plate. 

    Fold-increase in caspase activity can be determined by comparing these results with the level of the
    uninduced control. 

Further Categories Containing This Product:
Caspase Substrates • Panels / Sets
 
 
ALX-850-236 Revised 29-May-08
Caspase Inhibitor Set
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-236-KI01   1 Set 273.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Contains 25µl (2mM) each of the following inhibitors:
  - Caspase-1 Inhibitor, Z-YVAD-FMK, yellow cap
  - Caspase-3 Inhibitor, Z-DEVD-FMK, red cap
  - Caspase-6 Inhibitor, Z-VEID-FMK, violet cap
  - Caspase-8 Inhibitor, Z-IETD-FMK, green cap
  - Caspase-Family Inhibitor, Z-VAD-FMK, amber cap
FORMULATION: Liquid. In DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Synthetic peptides that irreversibly inhibit the activity of each of the caspase-family proteases. YVAD is the recognition sequence for caspase-1/ICE, DEVD is the recognition sequence for caspase-3/CPP32, VEID is the recognition sequence for caspase-6/Mch2, IETD is the recognition sequence for caspase-8/ FLICE, and VAD is the recognition sequence for a caspase(s) that functions upstream of most other caspases.   
General Information
BACKGROUND/TECHNICAL INFORMATION We recommend using 1000x dilutions for inhibiting caspase activity in Jurkat cell culture (e.g., add 1µl to 1ml of culture medium). Optimal doses may vary for different cells and culture conditions and must be determined individually.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Caspase Inhibitors • Panels / Sets
 
 
ALX-850-227 Revised 29-May-08
Caspase Inhibitor Set III
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-227-KI01   1 Set 441.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Contains 25µl (2mM) each of the following inhibitors:
  - Caspase-1 Inhibitor, Z-YVAD-FMK, yellow cap
  - Caspase-2 Inhibitor, Z-VDVAD-FMK, orange cap
  - Caspase-3 Inhibitor, Z-DEVD-FMK, red cap
  - Caspase-5 Inhibitor, Z-WEHD-FMK, clear cap
  - Caspase-6 Inhibitor, Z-VEID-FMK, violet cap
  - Caspase-8 Inhibitor, Z-IETD-FMK, green cap
  - Caspase-9 Inhibitor, Z-LEHD-FMK, blue cap
  - Caspase-Family Inhibitor, Z-VAD-FMK, amber cap
FORMULATION: Liquid. In DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Synthetic peptides that irreversibly inhibit the activity of each of the caspase-family proteases. YVAD is the recognition sequence for caspase-1/ICE, VDVAD is the recognition sequence for caspase-2, DEVD is the recognition sequence for caspase-3/CPP32, WEHD is the recognition sequence for caspase-5, VEID is the recognition sequence for caspase-6/Mch2, IETD is the recognition sequence for caspase-8/FLICE, LEHD is the recognition sequence for caspase-9, and VAD is the recognition sequence for a caspase(s) that functions upstream of most other caspases.
General Information
BACKGROUND/TECHNICAL INFORMATION We recommend using 1’000x dilutions for inhibiting caspase activity in Jurkat cell culture (e.g. add 1µl to 1ml of culture medium). Optimal doses may vary for different cells and culture conditions and must be determined individually.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Caspase Inhibitors • Panels / Sets
 
 
ALX-850-239 Revised 29-May-08
Caspase Inhibitor Set lV
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-239-KI01   1 Set 679.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Contains 25µl (2mM) each of the following inhibitors:
  - Caspase-1 Inhibitor, Z-YVAD-FMK, yellow cap
  - Caspase-2 Inhibitor, Z-VDVAD-FMK, orange cap
  - Caspase-3 Inhibitor, Z-DEVD-FMK, red cap
  - Caspase-4 Inhibitor, Z-LEVD-FMK, orange cap
  - Caspase-5 Inhibitor, Z-WEHD-FMK, clear cap
  - Caspase-6 Inhibitor, Z-VEID-FMK, violet cap
  - Caspase-8 Inhibitor, Z-IETD-FMK, green cap
  - Caspase-9 Inhibitor, Z-LEHD-FMK, blue cap
  - Caspase-10 Inhibitor, Z-AEVD-FMK, amber cap
  - Caspase-13 Inhibitor, Z-LEED-FMK, yellow cap
  - Caspase-Family Inhibitor, Z-VAD-FMK, amber cap
  - FMK Negative Control, Z-FA-FMK, clear cap
FORMULATION: Liquid. In DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description

Potent, cell permeable synthetic peptides that irreversibly inhibit activities of each of the caspase-family proteases. YVAD, VDVAD, DEVD, LEVD, WEHD, VEID, IETD, LEHD, and AEVD are the recognition sequences for caspase-1, -2, -3, -4, -5, -6, -8, -9, and -10. VAD is a general caspase inhibitor that inhibits all known caspases. Z-FA-FMK is a negative control for FMK inhibitors.

General Information
BACKGROUND/TECHNICAL INFORMATION We recommend using 1’000x dilutions for inhibiting caspase activity in Jurkat cell culture (e.g. add 1µl to 1ml of culture medium). Optimal doses may vary for different cells and culture conditions and must be determined individually
MANUFACTURER Manufactured by BioVision.
Related Products
Further Categories Containing This Product:
Caspase Inhibitors • Panels / Sets
 
 
ALX-850-237 Revised 07-Aug-08
Caspase Inhibitor Set Plus
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-237-KI01   1 Set 385.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Contains 25μl (2mM) each of the following inhibitors:
- Caspase-1 Inhibitor, Z-YVAD-FMK, yellow cap
- Caspase-2 Inhibitor, Z-VDVAD-FMK, orange cap
- Caspase-3 Inhibitor, Z-DEVD-FMK, red cap
- Caspase-6 Inhibitor, Z-VEID-FMK, violet cap
- Caspase-8 Inhibitor, Z-IETD-FMK, green cap
- Caspase-9 Inhibitor, Z-LEHD-FMK, blue cap
- Caspase-Family Inhibitor, Z-VAD-FMK, amber cap
FORMULATION: Liquid. In DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
Product Description
Synthetic peptide inhibitors that irreversibly inhibit the activity of each of the caspase-family proteases. YVAD is the recognition sequence for caspase-1, VDVAD is the recognition sequence for caspase-2, DEVD is the recognition sequence for caspase-3, VEID is the recognition sequence for caspase-6, IETD is the recognition sequence for caspase-8, LEHD is the recognition sequence for caspase-9, and VAD is the recognition sequence for a caspase(s) that functions upstream of most other caspases.
General Information
BACKGROUND/TECHNICAL INFORMATION We recommend using 1’000x dilutions for inhibiting caspase activity in Jurkat cell culture (e.g., Add 1µl to 1ml of culture medium). Optimal doses may vary for different cells and culture conditions and must be determined individually.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Caspase Inhibitors • Panels / Sets
 
 
ALX-201-056 Revised 21-Jul-08
Caspase-1 (active) (human) (recombinant)
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SYNONYMS Interleukin 1β Converting Enzyme (active) (human) (recombinant)
IL-1β Converting Enzyme (active) (human) (recombinant)
ICE (active) (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-056-U025   25 U 231.00 USD Add To Cart
ALX-201-056-U100   100 U 385.00 USD Add To Cart
Product Specification
EC: 3.4.22.36
SOURCE/HOST: Produced in E. coli.
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY: ~20’000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate YVAD-pNA (pNA: p-nitroanaline) per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM sodium chloride, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: Useful in studying enzyme regulation and inhibitors, determining target substrate, screening caspase inhibitors, or as a positive control in caspase assays. We recommend using 1 unit per assay for analyzing caspase activity.
QUALITY CONTROL: Routinely tested for its ability to cleave the caspase-1 colorimetric substrate Ac-YVAD-pNA (Prod. No. ALX-260-026).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
Caspase-1 is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20kDa) and small (10kDa) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P29466: Caspase-1 (human) (precursor)
AfCS Signalling Gateway link A000492: Caspase-1 (mouse)
Further Categories Containing This Product:
Enzymes • Inflammasome • Recombinant Proteins / Fusion Proteins
 
 
ALX-850-211 Revised 22-Apr-08
Caspase-1 Colorimetric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-211-KI01   25 tests 175.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl YVAD-pNA (1mM), 100µl DTT (1M), 25ml Dilution Buffer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening.
Product Description
Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate YVAD-pNA.
General Information
The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from an treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
MANUFACTURER

Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome • Activity Assays
 
 
ALX-850-212 Revised 21-Apr-08
Caspase-1 Fluorometric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-212-KI01   25 tests 175.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2x Reaction Buffer, 125µl YVAD-AFC Substrate (1mM), 100µl DTT (1M).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate YVAD-AFC.
General Information
YVAD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-1 activity.
MANUFACTURER

Manufactured by BioVision.
Further Categories Containing This Product:
Inflammasome • Activity Assays
 
 
ALX-201-057 Revised 30-Jun-08
Caspase-2 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-057-U025   25 U 203.00 USD Add To Cart
ALX-201-057-U100   100 U 371.00 USD Add To Cart
Product Specification
EC: 3.4.22.36
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY: 10'000-13'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate VDVAD-pNA per hour at 37°C in reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: Useful in screening capase inhibitors, studying enzyme regulation and kinetics, determining specificity of capase substrates, or as positive control in caspase activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
For a complete caspase-2 assay protocol, please refer to Caspase-2 Fluorometric or Colorimetric Assay Kits (Prod. No. ALX-850-214 or ALX-850-213).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Images
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General Information
Caspase-2 is a member of the interleukin-1β converting enzyme (ICE) family of cysteine proteases. Similar as other caspases, caspase-2 also exists in cells as an inactive proenzyme. During apoptosis, procaspase-2 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases. The processed form of caspase-2 consists of large (19kDa) and small (12kDa) subunits which associate to form the active enzyme. The expressed caspase-2 spontaneously undergoes auto-processing to yield the subunits characteristic of the native enzyme. The active recombinant caspase-2 is routinely tested for its ability to enzymatically cleave the caspase-2 colorimetric substrate Ac-VDVAD-pNA (Prod. No. ALX-260-059). The signal can be detected by spectrophotometry.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P42575: Caspase-2 (human) (precursor)
AfCS Siganlling Gateway link A000497: Caspase-2 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-083 Revised 24-Mar-05
Caspase-2 (active) (human) (recombinant) (high purity)
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SYNONYMS ICH-1 (active) (human) (recombinant) (high purity)
CASP-2 (active) (human) (recombinant) (high purity)
ICH-1 protease (active) (human) (recombinant) (high purity)
ICH-1L/1S (active) (human) (recombinant) (high purity)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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ALX-522-083-C005   5 µg 180.00 USD Add To Cart
Product Specification