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ALX-210-245
Revised 21-Nov-06
Polyclonal Antibody to Ah Receptor Nuclear Translocator 3
SYNONYMS
anti-ARNT3 PAb
anti-BMAL1 PAb
anti-Brain & Muscle ARNT-like 1 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Aryl Hydrocarbon Receptor / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-245-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 582-594 (CD
582
MIDNDQG
SSSPS
594
) of mouse ARNT3 (Ah receptor nuclear translocator 3).
SPECIFICITY:
Recognizes human and hamster ARNT3.
APPLICATION:
Western Blot (1:200)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-152-023
.
General Information
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Transactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. CLOCK-BMAL1 has been shown to transactivate the mammalian homolog of Drosophila per. PER, in concert with the product of the mammalian the timeless gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Further Categories Containing This Product:
Circadian Transcriptional Regulators / Related Products
Polyclonal Antibodies
ALX-210-246
Revised 14-Sep-06
Polyclonal Antibody to PER1 (mouse)
SYNONYMS
anti-Period Circadian Protein 1 (mouse) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcriptional Repressors & Corepressors / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-246-R200
200 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. Epitope-affinity purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 39-51 (P
39
SLADDTDANSNG
51
) of mouse PER1 (period circadian protein 1).
SPECIFICITY:
Recognizes mouse PER1.
APPLICATION:
Immunohistochemistry (frozen and paraffin sections (1:500))
Western Blot (1:200)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-165-043
.
Product Description
Circadian rhythmicity is a basic property of phylogenetically diverse organisms which range from animals and plants, to fungi. Regulation of endogenous biological clocks is regulated at the genetic level by a protein mediated autoregulatory feed-back loop. In mammals, several genes that encode members of the basic helix-loop helix (bHLH) PAS (PER-ARNT-SIM) transcription factor family have been shown to play a significant role in regulating circadian oscillations. Tranasactivation of CLOCK-induced genes is mediated via an E box enhancer (CACGTG) found upstream of target genes. CLOCK-BMAL1 heterodimers bind to E box regulatory elements and stimulate gene transcription. PER, when complexed with the product of the mammalian
timeless
gene (TIM), negatively regulates its own transcription by blocking the activity of the CLOCK-BMAL1 transactivation complex.
Further Categories Containing This Product:
Circadian Transcriptional Regulators / Related Products
Polyclonal Antibodies
ALX-210-254
Revised 01-Feb-07
Polyclonal Antibody to N-CoR
SYNONYMS
anti-Nuclear Receptor Co-repressor PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
SMRT & N-CoR / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-254-C050
50 µg
524.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.7mg/ml
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 2437-2453 (CP
2437
APLLSAQYETLSDSDD
2453
) of mouse N-CoR (nuclear receptor co-repressor).
SPECIFICITY:
Recognizes human and mouse N-CoR.
APPLICATION:
Immunoprecipitation, Chromatin Immunoprecipitation
Western Blot (1µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-163-007
.
General Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. SMRT (silencing mediator of retinoic acid & thyroid hormone receptor) and N-CoR (nuclear receptor corepressor) are related transcriptional corepressors which contain two distinct domains capable of interacting with unliganded nuclear receptors to repress their basal transcriptional activity.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-255
Revised 25-Sep-06
Polyclonal Antibody to AIB1 (human)
SYNONYMS
anti-Amplified in Breast Cancer 1 (human) PAb
anti-SRC-3 (human) PAb
anti-Steroid Receptor Coactivator-3 (human) PAb
anti-NCOA3 (human) PAb
anti-Nuclear Receptor Coactivator 3 (human) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Nuclear Receptor Coactivators / Related Products
Ordering Information
Product Numbers:
Format:
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Unit Price:
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ALX-210-255-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 3-15 (G
3
LGENLDPLASDS
15
C) of human AIB1 (amplified in breast cancer 1).
SPECIFICITY:
Recognizes human AIB1. Detects a band of ~155kDa by Western blot.
APPLICATION:
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-151-034
.
General Information
Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. AIB1 (Amplified In Breast Cancer-1) is a member of a growing family of transcriptional coactivator proteins which includes SRC-1 and GRIP1.
AIB1 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, progesterone, and peroxisome proliferator activated receptors. AIB1 also interacts with the transcriptional integrators CREB binding protein (CBP) and p300.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-256
Revised 17-Nov-08
Polyclonal Antibody to Histone Deacetylase 1
SYNONYMS
anti-HDAC1 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
HDAC Antibodies
Ordering Information
Product Numbers:
Format:
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ALX-210-256-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 467-482 (CE
467
EKPEAK
GVKEEVKLA
482
) of human HDAC1 (histone deacetylase 1).
SPECIFICITY:
Recognizes human, mouse, rat, hamster and dog HDAC1. Detects a band of ~62kDa by Western blot.
APPLICATION:
Immunocytochemistry (2µg/ml)
Immunohistochemistry (frozen sections (0.2µg/ml))
Immunoprecipitation, Chromatin Immunoprecipitation
Western Blot (0.5µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-158-006
.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription:
Y. Ling, et al.; Nucl. Acids Res.
32
, 598 (2004)
Abstract
;
Full Text
General Information
Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA from the inaccessible, tightly packed nucleosomal particles to the accessible, unwound nucleosomal particles. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. A single nucleosomal core particle contains an octamer of histone proteins H2A, H2B, H3 and H4. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Several known steroid receptor coactivators in the p160 family, such as SRC-1 and AIB1, possess histone acetyltransferase (HAT) activity, which is thought to be critical for function. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs. Class I proteins are related to Rpd3 and members of class II resemble Hda1p.
HDAC1 is a class I histone deacetylase containing 482 amino acid residues. HDAC1 has been shown to interact directly with transcription factors and has been shown to deacetylate histone proteins H3 and H4.
General Literature References
A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p :
J. Taunton, et al.; Science
272
, 408 (1996)
Abstract
A role for histone deacetylase activity in HDAC1-mediated transcriptional repression:
C.A. Hassig, et al.; PNAS
95
, 3519 (1998)
Abstract
The COOH-terminal region of pRb2/p130 binds to histone deacetylase 1 (HDAC1), enhancing transcriptional repression of the E2F-dependent cyclin A promoter :
P. Stiegler, et al.; Cancer Res.
58
, 5049 (1998)
Abstract
Related Products
ALX-158-006
Blocking Peptide for PAb to Histone Deacetylase 1 (Prod. No.
ALX-210-256
)
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-257
Revised 15-May-06
Polyclonal Antibody to Histone Deacetylase 2
SYNONYMS
anti-HDAC2 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
HDAC Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-257-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Dog
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 475-488 (CT
475
DTKGTKS
EQLSNP
488
) of human HDAC2 (histone deacetylase 2).
SPECIFICITY:
Recognizes human, mouse, rat, hamster and dog HDAC2. Detects a band of ~55kDa by Western blot. Also detects a band of ~40KDa of unknown identity.
APPLICATION:
Flow Cytometry
Immunocytochemistry (1µg/ml)
Immunohistochemistry (0.4µg/ml)
Immunoprecipitation
Western Blot (0.5µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-158-007
.
Product Specific Literature References
Modification of de novo DNA methyltransferase 3a (Dnmt3a) by SUMO-1 modulates its interaction with histone deacetylases (HDACs) and its capacity to repress transcription:
Y. Ling, et al.; Nucl. Acids Res.
32
, 598 (2004)
Abstract
;
Full Text
General Literature References
Roles of histone acetyltransferases and deacetylases in gene regulation :
M.H. Kuo & C.D. Allis; Bioessays
20
, 615 (1998), (Review)
Abstract
Cloning and characterization of the mouse histone deacetylase-2 gene
:
Y. Zeng, et al.; J. Biol. Chem.
273
, 28921 (1998)
Abstract
;
Full Text
Ligand-induced recruitment of a histone deacetylase in the negative- feedback regulation of the thyrotropin beta gene :
S. Sasaki, et al.; EMBO J.
18
, 5389 (1999)
Abstract
;
Full Text
Related Products
ALX-158-007
Blocking Peptide for PAb to Histone Deacetylase 2 (Prod. No.
ALX-210-257
)
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-258
Revised 28-Aug-06
Polyclonal Antibody to Histone Deactetylase 3
SYNONYMS
anti-HDAC3 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
HDAC Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-258-C100
100 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Dog
Others
SOURCE/HOST:
From rabbit.
CONCENTRATION:
1mg/ml.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 415-428 (CD
415
GDHDNDK
ESDVEI
428
) of human HDAC3 (histone deacetylase 3).
SPECIFICITY:
Recognizes human, rat, hamster and dog HDAC3. Detects a band of ~48kDa by Western blot.
APPLICATION:
Western Blot (2µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
BLOCKING PEPTIDE:
For Blocking Peptide see Prod. No.
ALX-158-008
.
Product Specific Literature References
Characterization of a human RPD3 ortholog, HDAC3:
S. Emiliani, et al.; PNAS
95
, 2795 (1998)
Abstract
;
Full Text
Effect of estradiol on histone acetylation dynamics in human breast cancer cells:
J.M. Sun, et al.; J. Biol. Chem.
276
, 49435 (2001)
Abstract
;
Full Text
The transcriptional repressor Sp3 is associated with CK2-phosphorylated histone deacetylase 2:
J.M. Sun, et al.; J. Biol. Chem.
277
, 35783 (2002)
Abstract
;
Full Text
Identification of a novel family of ankyrin repeats containing cofactors for p160 nuclear receptor coactivators:
A. Zhang, et al.; J. Biol. Chem.
279
, 33799 (2004)
Abstract
;
Full Text
Related Products
ALX-158-008
Blocking Peptide for PAb to Histone Deacetylase 3 (Prod. No.
ALX-210-258
)
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-261
Revised 25-Jun-08
Polyclonal Antibody to MCAD
SYNONYMS
anti-Medium-chain Fatty Acyl-CoA Dehydrogenase PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PPAR Other Products
Ordering Information
Product Numbers:
Format:
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ALX-210-261-1
1 Vial
313.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Sheep
Pig
SOURCE/HOST:
From rabbit.
CONCENTRATION:
0.6mg/ml
PURITY DETAIL:
Affinity purified.
FORMULATION:
Liquid. In 500μl TBS, pH 7.4, containing 50% glycerol, 0.5mg/ml BSA and 0.02% sodium azide.
IMMUNOGEN:
Recombinant human MCAD (medium-chain fatty acyl-CoA dehydrogenase).
SPECIFICITY:
Recognizes human, mouse, pig and sheep MCAD. Detects a band of ~43kDa by Western blot.
APPLICATION:
Western Blot (1:200)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Product Specific Literature References
The tissue-specific expression and developmental regulation of two nuclear genes encoding rat mitochondrial proteins. Medium chain acyl-CoA dehydrogenase and mitochondrial malate dehydrogenase:
D.P. Kelly, et al.; J. Biol. Chem.
264
, 18921 (1989)
Abstract
;
Full Text
Fatty acid oxidation enzyme gene expression is downregulated in the failing heart:
M.N. Sack, et al.; Circulation
94
, 2837 (1996)
Abstract
;
Full Text
A role for estrogen-related receptor alpha in the control of mitochondrial fatty acid beta-oxidation during brown adipocyte differentiation:
R.B. Vega & D.P. Kelly; J. Biol. Chem.
272
, 31693 (1997)
Abstract
;
Full Text
A role for Sp and nuclear receptor transcription factors in a cardiac hypertrophic growth program:
M.N. Sack, et al.; PNAS
94
, 6438 (1997)
Abstract
;
Full Text
General Information
Medium-chain fatty acyl-CoA dehydrogenase (MCAD) is a mitochondrial enzyme that catalyzes the first step in the β-oxidation of fatty acids. MCAD expression is induced during periods of fasting, when reliance on fatty acids for energy is increased [1,2]. The promoter for MCAD contains a peroxisome proliferator response element (PPRE) and is regulated transcriptionally by peroxisome proliferator-activated receptor alpha (PPARα), a ligand-activated transcription factor involved in the regulation of lipid homeostasis [1,3,4]. Because of this, MCAD expression can be used as a marker to evaluate the
in vivo
activity of PPARα [1,2]. Human MCAD is approximately 87% homologous to pig and rat MCAD, respectively [5, 6]. MCAD is expressed in liver, heart, kidney, and skeletal muscle.
General Literature References
[1]
The peroxisome proliferator-activated receptor regulates mitochondrial fatty acid oxidative enzyme gene expression
:
T. Gulick, et al.; PNAS
91
, 11012 (1994)
Abstract
;
Full Text
[2]
A critical role for the peroxisome proliferator-activated receptor alpha (PPARalpha) in the cellular fasting response: the PPARalpha-null mouse as a model of fatty acid oxidation disorders
:
T.C. Leone, et al.; PNAS
96
, 7473 (1999)
Abstract
;
Full Text
[3]
A pleiotropic element in the medium-chain acyl coenzyme A dehydrogenase gene promoter mediates transcriptional regulation by multiple nuclear receptor transcription factors and defines novel receptor-DNA binding motifs
:
M.E. Carter, et al.; Mol. Cell. Biol.
14
, 4360 (1994)
Abstract
[4]
Peroxisome proliferator-activated receptors: a nuclear receptor signaling pathway in lipid physiology
:
T. Lemberger, et al.; Annu. Rev. Cell Dev. Biol.
12
, 335 (1996), (Review)
Abstract
[5]
Nucleotide sequence of medium-chain acyl-CoA dehydrogenase mRNA and its expression in enzyme-deficient human tissue
:
D.P. Kelly, et al.; PNAS
84
, 4068 (1987)
Abstract
[6]
Molecular cloning and nucleotide sequence of cDNA encoding the entire precursor of rat liver medium chain acyl coenzyme A dehydrogenase
:
Y. Matsubara, et al.; J. Biol. Chem.
262
, 10104 (1987)
Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-277
Revised 14-Feb-07
Polyclonal Antibody to Glucocorticoid Receptor α
SYNONYMS
anti-GRα PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Glucocorticoids & Glucocorticoid Receptors / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-277-C050
50 µg
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
PURITY DETAIL:
Epitope-affinity purified IgG.
FORMULATION:
Lyophilized. Contains 0.05% sodium azide.
RECONSTITUTION:
Reconstitute with PBS.
IMMUNOGEN:
Synthetic peptide corresponding to aa 755-771 (C
755
EIITNQIPKYSN
GNIKK
771
) of human GRα (glucocorticoid receptor α
)
.
SPECIFICITY:
Recognizes human, mouse and rat GRα. Detects a band of ~94kDa by Western blot.
APPLICATION:
Immunoprecipitation, Chromatin Immunoprecipitation
Western Blot (4µg/ml)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-283
Revised 06-Apr-06
Polyclonal Antibody to SRC-1
SYNONYMS
