ALEXIS Biochemicals: TL1A, Soluble (human) Detection Set [For ELISA Application]

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APO-54N-022

Revised 05-Dec-08

BAFF, Soluble (human) Detection Set [For ELISA Application]
SYNONYMS	BLyS, Soluble (human) Detection Set [For ELISA Application] TALL-1, Soluble (human) Detection Set [For ELISA Application] zTNF4, Soluble (human) Detection Set [For ELISA Application] TNFSF 13B/20, Soluble (human) Detection Set [For ELISA Application] CD257, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-022-KI01		1 Set	560.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.3ng/ml (range 0 to 30ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD) 1 vial Coating Antibody (COAT) 1 vial Detection Antibody (DET) 1 vial Goat anti-rat IgG-HRP (HRP)
APPLICATION:	For the quantitative determination of soluble human BAFF from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse BAFF.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) and the goat anti-rat IgG1-HRP (HRP) at -20°C.

General Information

BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form, leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9Y275: BAFF (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

The TNF family members BAFF and APRIL: the growing complexity: F. Mackay & C. Ambrose; Cytokine Growth Factor Rev. 14, 311 (2003) Abstract
TNF deficiency fails to protect BAFF transgenic mice against autoimmunity and reveals a predisposition to B cell lymphoma: M. Batten, et al.; J. Immunol. 172, 812 (2004) Abstract
BAFF Augments Certain Th1-Associated Inflammatory Responses: A.P. Sutherland, et al.; J. Immunol. 174, 5537 (2005) Abstract; Full Text
The BAFF/APRIL system: life beyond B lymphocytes: L.G. Ng, et al.; Mol. Immunol. 42, 763 (2005) Abstract
The BAFF/APRIL system: an important player in systemic rheumatic diseases: F. Mackay, et al.; Curr. Dir. Autoimmun. 8, 243 (2005) Abstract

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ELISA Sets

APO-54N-013

Revised 05-Dec-08

BAFF, Soluble (mouse) Detection Set [For ELISA Application]
SYNONYMS	BLyS, Soluble (mouse) Detection Set [For ELISA Application] TALL-1, Soluble (mouse) Detection Set [For ELISA Application] zTNF4, Soluble (mouse) Detection Set [For ELISA Application] TNFSF 13B/20, Soluble (mouse) Detection Set [For ELISA Application] CD257, Soluble (mouse) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BAFF

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-013-KI01		1 Set	1'050.00 USD

Product Specification

SENSITIVITY:	0.3ng/ml (range 0 to 20ng/ml)
QUANTITY:	For 4x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of soluble mouse BAFF from biological fluids (serum and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

Product Images

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Product Specific Literature References

Toll-like Receptor 9-Dependent and -Independent Dendritic Cell Activation by Chromatin-Immunoglobulin G Complexes: M.W. Boule, et al.; J. Exp. Med. 199, 1631 (2004) Abstract
Regulation of B-cell survival by BAFF-dependent PKCdelta-mediated nuclear signalling: I. Mecklenbrauker, et al.; Nature 431, 456 (2004) Abstract
BAFF Augments Certain Th1-Associated Inflammatory Responses: A.P. Sutherland, et al.; J. Immunol. 174, 5537 (2005) Abstract; Full Text
A role for B cell-activating factor of the TNF family in chemically induced autoimmunity: Y. Zheng, et al.; J. Immunol. 175, 6163 (2005) Abstract
Development of autoantibody responses in NC/Nga mice: its prevention by pulverized konjac glucomannan feeding: N. Onishi, et al.; Arch. Dermatol. Res. 300, 95 (2008) Abstract

General Information

BAFF (B cell activation factor of the TNF family, also known as BLyS or TALL1) is a cytokine expressed predominantly by cells of the immune system such as neutrophils, monocytes, macrophages, dendritic cells, follicular dendritic cells, activated T cells and some malignant B cells. BAFF binds three distinct receptors (BAFF-R, TACI and BCMA) expressed predominantly on B cells, although activated T cells also express BAFF-R. BAFF is a master regulator of peripheral B cell survival, and also acts in processes such as immunoglobulin isotype switch and B cell co-stimulation. Besides its major role in B cell biology, BAFF co-stimulates activated T cells. Deregulated expression of this membrane-bound protein, which can readily be released in a soluble form, leads to autoimmune disorders in mice. In humans, elevated levels of soluble BAFF have been detected in the serum of patients with various autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link : BAFF (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

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ELISA Sets

ALX-850-302/1

Revised 22-Dec-08

CD14, Soluble (human) ELISA Kit
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	CD14 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-302/1-KI01		1 Kit	650.00 USD

Product Specification

SENSITIVITY:	Range: 5-50ng/ml
QUANTITY:	96 wells (~80 tests)
KIT/SET CONTAINS:	1 precoated ELISA module 1 vial detecting antibody (POD-labelled monoclonal antibody to human CD14) (ready-to-use) 1 vial human CD14 standard (recombinant human CD14, lyophilized) 1 vial reference serum (2.7 ± 0.5, lyophilized) 2 tablets PBS 1 vial dilution buffer 1 vial Tween 20 1 vial stopping solution (ready-to-use) 1 vial substrate solution (ready-to-use)
APPLICATION:	For the quantitative determination of soluble human CD14 in serum, plasma and cell culture medium.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep vials 3 and 4 at -20°C.

General Information

MANUFACTURER

Manufactured by Biometec.

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ELISA Sets

ALX-850-303/1

Revised 19-Dec-08

CD14, Soluble (mouse) ELISA Kit
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	CD14 / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-850-303/1-KI01		1 Kit	870.00 USD

Product Specification

SENSITIVITY:	Range: 5-50ng/ml
QUANTITY:	96 wells (~80 tests)
KIT/SET CONTAINS:	1 precoated ELISA module 1 vial detecting antibody (POD-labelled polyclonal antibodies to mouse CD14) 1 vial mouse CD14 standard (recombinant mouse CD14, lyophilized) 1 vial reference serum (2.7 ± 0.5, lyophilized) 2 tablets PBS 1 vial sample dilution buffer 1 vial Tween 20 1 vial stopping solution (ready-to-use) 1 vial substrate solution (reday-to-use) 1 vial detecting antibody dilution buffer
APPLICATION:	For the quantitative determination of soluble mouse CD14 in serum, plasma and cell culture medium.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep vials 3 and 4 at -20°C.

General Information

MANUFACTURER

Manufactured by Biometec.

Further Categories Containing This Product:

ELISA Sets

APO-54N-040

Revised 05-Dec-08

Chemerin (human) Detection Set [For ELISA Application]
SYNONYMS	RARRES2 (human) Detection Set [For ELISA Application] Retinoic Acid Receptor Responder Protein 2 (human) Detection Set [For ELISA Application] TIG2 (human) Detection Set [For ELISA Application] Tazarotene-induced Gene 2 (human) Detection Set [For ELISA Application] RAR-responsive Protein (human) Detection Set [For ELISA Application]
PRODUCT LINE	Obesity & Adipokines
PRODUCT CATEGORY	Adipokines Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-040-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.1ng/ml (range 0.156 to 10ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human chemerin from biological fluids (serum, plasma and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

General Information

Chemerin (tazarotene-induced gene 2; TIG2) is a chemotactic agent that was identified as the ligand for the orphan G protein-coupled receptor ChemR23. Recently, chemerin has been shown to be expressed abundantly in adipose tissue and to function as a new adipokine [1, 2]. Chemerin potentiates insulin-stimulated glucose uptake and regulates adipogenesis and adipocyte metabolism [1].
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Chemerin, a novel adipokine that regulates adipogenesis and adipocyte metabolism: K.B. Goralski, et al.; J. Biol. Chem. 282, 28175 (2007) Abstract; Full Text
[2] Chemerin is a novel adipokine associated with obesity and metabolic syndrome: K. Bozaoglu, et al.; Endocrinology 148, 4687 (2007) Abstract

Related Products

ALX-804-835	Monoclonal Antibody to CTRP7 (Anna-1)
ALX-210-924	Polyclonal Antibody to CTRP7 (AT103)

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ELISA Sets

APO-54N-043

Revised 08-Dec-08

HMGB1 (human) Detection Set [For ELISA Application]
SYNONYMS	High Mobility Group Protein B1 (human) Detection Set [For ELISA Application]
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Non-apoptotic Cell Death / Necrosis

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-043-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	0.6ng/ml (range 0.78 to 50ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human HMGB1 from biological fluids (serum and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

General Information

HMGB1 is present in almost all mammalian nuclei where it functions in many nuclear processes including gene transcription. Post-translational methylation at lysine 42 (Lys⁴²) of HMGB1 causes its cytoplasmic localization in neutrophils [1]. HMGB1 is also found extracellularly after passive release by necrotic cells or through active secretion in response to angiogenic and inflammatory signals [2, 3]. Apoptotic cells also release HMGB1 but in an oxidized form that does not trigger the immune response [4]. Secreted HMGB1 may signal through the receptor for advanced glycation end products (RAGE), and via toll-like receptors, TLR2 and TLR4 [5]. HMGB1 functions as a late mediator of immune response [3] : it is released from dying tumor cells, stimulates mDC tumor antigen processing through its interaction with TLR4 [6]. HMGB1 is overexpressed in a number of cancers where the protein contributes to the development of tumors [7].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P09429: HMGB1 (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Post-translational methylation of high mobility group box 1 (HMGB1) causes its cytoplasmic localization in neutrophils: I. Ito, et al.; J. Biol. Chem. 282, 16336 (2007) Abstract; Full Text
[2] HMGB1: a signal of necrosis: A. Raucci, et al.; Autoimmunity 40, 285 (2007) Abstract
[3] Pattern recognition receptors in the immune response against dying cells: P. Jeannin, et al.; Curr. Opin. Immunol. 20, 530 (2008) Abstract
[4] Induction of immunological tolerance by apoptotic cells requires caspase-dependent oxidation of high-mobility group box-1 protein: H. Kazama, et al.; Immunity 29, 21 (2008) Abstract
[5] Convergence and amplification of toll-like receptor (TLR) and receptor for advanced glycation end products (RAGE) signaling pathways via high mobility group B1 (HMGB1): J.R. van Beijnum, et al.; Angiogenesis 11, 91 (2008) Abstract
[6] Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy: L. Apetoh, et al.; Nat. Med. 13, 1050 (2007) Abstract
[7] Masquerader: high mobility group box-1 and cancer: J.E. Ellerman, et al.; Clin. Cancer Res. 13, 2836 (2007) Abstract

Further Categories Containing This Product:

ELISA Sets • DNA Regulation / Transcription Other Products

APO-54N-025

Revised 05-Dec-08

IL-33, Soluble (human) Detection Set [For ELISA Application]
SYNONYMS	Interleukin-33, Soluble (human) Detection Set [For ELISA Application] NF-HEV, Soluble (human) Detection Set [For ELISA Application] IL-1F11, Soluble (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Interleukin Kits & Sets

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-025-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	5pg/ml (range 0 to 500pg/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of soluble human IL-33 from biological fluids (serum and cell culture supernatant). Does not cross-react with mouse IL-33.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

Product Images

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General Information

Interleukin-1 (IL-1) family members, such as IL-1α/β and IL-18 play important roles in host defense, immune regulation, neuronal injury and inflammation [1, 2]. Recently, a new member of this family, IL-33, also named Nuclear Factor from High Endothelial Venules (NF-HEV) [3], has been reported to be the specific ligand for ST2 [4], an orphan receptor of the IL-1 receptor family [5]. Structurally, IL-33 is closest to IL-18, rather than IL-1β. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kDa mature protein (112-270) [6]. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK athways. IL-33 is synthesized in response to cytokines such as IL-1β (but also TNFα) and plays an important role in T_H2-associated immunology, by triggering an increase secretion of IL-5 and IL-13 from polarized T_H2 cells. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q2YEJ5: Interleukin-33 (human)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Differences in signaling pathways by IL-1beta and IL-18: J.K. Lee, et al. ; Proc. Natl. Acad. Sci. 101, 8815 (2004) Abstract
[2] Interleukin-1 and neuronal injury: S.M. Allan, et al.; Nat. Rev. Immunol. 5, 629 (2005) Abstract
[3] Molecular characterization of NF-HEV, a nuclear factor preferentially expressed in human high endothelial venules: E.S. Baekkevold, et al. ; Am. J. Pathol. 163, 69 (2003) Abstract
[4] IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005) Abstract
[5] ST2 is an inhibitor of interleukin 1 receptor and Toll-like receptor 4 signaling and maintains endotoxin tolerance: E.K. Brint, et al. ; Nat. Immunol. 5, 373 (2005) Abstract
[6] Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases: F. Martinon & J. Tschopp; Cell 117, 561 (2004) Abstract

Further Categories Containing This Product:

Interleukin-33 [IL-33] & Receptors / Related Products • ELISA Sets

APO-54N-032

Revised 05-Dec-08

ILEI (human) Detection Set [For ELISA Application]
SYNONYMS	Interleukin-like EMT Inducer (human) Detection Set [For ELISA Application] FAM3C (human) Detection Set [For ELISA Application]
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Chemokines & Cytokines Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
APO-54N-032-KI01		1 Set	630.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
SENSITIVITY:	200 pg/ml (range 0 to 20 ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human ILEI from biological fluids (serum, plasma and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

General Information

In 2006, T. Waerner, et al. characterized ILEI as a key player in both epithelial to mesenchymal transition and late-stage carcinoma progression. EMT is a process that involves changes in gene expression, cytoskeleton organization, cell adhesion, and extracellular matrix composition. This process is important during tissue formation and organogenesis, but also during cancer progression, metastasis and chronic inflammation. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors, an event strongly correlated to invasion/EMT, metastasis formation, and survival in human colon and breast cancer.

ALX-804-869	Monoclonal Antibody to Chemerin (Tigy-2)
ALX-804-868	Monoclonal Antibody to Chemerin (Tigy-1)
ALX-522-142	Chemerin (human) (recombinant)

SPECIES CROSSREACTIVITY:	Human Mouse
SENSITIVITY:	0.1ng/ml (range 0 to 10ng/ml).
QUANTITY:	For 5x96 wells.
KIT/SET CONTAINS:	1 vial Standard (lyophilized) (STD). 1 vial Coating Antibody (COAT). 1 vial Detection Antibody (DET).
APPLICATION:	For the quantitative determination of human and mouse CTRP7 from biological fluids (serum and cell culture supernatant).
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	Avoid freeze/thaw cycles. For long term storage keep the standard (STD) at -20°C.

Adipose tissue functions as storage of energy and plays an active role in monitoring and controlling whole-body metabolism by secreting a number of proinflammatory adipokines, (such as leptin, resistin, tumor necrosis factor-α and interleukin-6), as well as one anti-inflammatory adipokine, adiponectin. Adiponectin is involved in controlling the whole-body metabolism, particularly by increasing insulin sensitivity in muscle and liver, and by increasing fatty acid oxidation in muscle [1]. Recently, a new highly conserved family of adiponectin paralogs designated as C1q/Tumor Necrosis Factor-α-Related Proteins (CTRPs) 1-7, has been described in mouse and human [2-5]. This family of proteins exhibits similar structural and biological properties to adiponectin. The CTRPs, unlike adiponectin whose expression is restricted to adipose tissue, are widely expressed by many different adult tissues. Like adiponectin, CTRP2 rapidly induces acetyl-CoA carboxylase, and mitogen-activated protein kinase phosphorylation in C2C12 myotubes, which resulted in increased glycogen accumulation and fatty acid oxidation, and CTRP7 (as well as CTRPs 1, 2 and 3) induces AMP-activated protein kinase phosphorylation. The activity of the CTRPs suggests that some members of the CTRP family of proteins could substitute for adiponectin function in vivo.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q8BVD7: CTRP7 (mouse) Swiss-Prot link Q9BXJ2: CTRP7 (human)
MANUFACTURER	Manufactured by Apotech Corporation.