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RANK & RANKL
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ALX-522-131 Revised 27-Mar-08
Fc (human):RANKL, Soluble (mouse) (rec.)
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SYNONYMS Fc (human):ODF, Soluble (mouse) (recombinant)
Fc (human):OPGL, Soluble (mouse) (recombinant)
Fc (human):TRANCE, Soluble (mouse) (recombinant)
Fc (human):TNFSF 11, Soluble (mouse) (recombinant)
Fc (human):CD254, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-131-C010   10 µg 390.00 USD Add To Cart
Product Specification
MW: ~55kDa
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse RANKL (aa 157-316) is fused to the Fc portion of human IgG1.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to mouse RANK.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O35235: TNF11 (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-804-212 Revised 08-Dec-08
Monoclonal Antibody to RANK (human) (9A725)
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SYNONYMS anti-TRANCE-R (human) MAb (9A725)
anti-ODFR (human) MAb (9A725)
anti-TNFRSF 11A (human) MAb (9A725)
anti-CD265 (human) MAb (9A725)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-804-212-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 9A725
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant human RANK (aa 326-616).
SPECIFICITY: Recognizes an epitope between aa 330-427 of human RANK. Detects a band of ~97kDa by Western blot.
APPLICATION: Flow Cytometry (intracellular) (see [1])
Immunohistochemistry (paraffin sections)
Western Blot  (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
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Product Specific Literature References
[1] Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
[2] A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004) Abstract
[3] Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism: A.C. Bharti, et al.; J. Biol. Chem. 279, 6065 (2004) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-244 Revised 20-Nov-08
Monoclonal Antibody to RANKL (12A380)
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SYNONYMS anti-ODF MAb (12A380)
anti-OPGL MAb (12A380)
anti-TRANCE MAb (12A380)
anti-TNFSF 11 MAb (12A380)
anti-CD254 MAb (12A380)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-804-244-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 12A380
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant mouse RANKL (aa 1-317).
SPECIFICITY: Recognizes human and mouse RANKL. Detects a band of ~40kDa by Western blot.
APPLICATION: Flow Cytometry (see [2])
Immunohistochemistry (frozen sections (see [2]))
Western Blot (4-6µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
Product Specific Literature References
[1] Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
[2] Estrogen deficiency accelerates murine autoimmune arthritis associated with receptor activator of nuclear factor-kappa B ligand-mediated osteoclastogenesis: T. Yoneda, et al.; Endocrinology 145, 2384 (2004) Abstract; Full Text
[3] Expression of bone-regulatory proteins in human valve allografts: R. Shetty, et al.; Heart 92, 1303 (2006) Abstract; Full Text
General Information
RANKL (TRANCE, OPGL, ODF) is a member of the TNF family. RANKL (receptor activator of NF-κB [RANK] ligand) or TRANCE (TNF-related activation-induced cytokine receptor) has been implicated in interactions between T cells and dendritic cells [1,2]. RANKL binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival [2]. RANKL is also important in T- and B-cell maturation [3]. As OPGL (osteoprotegerin ligand) or ODF (osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis [4,5]. RANKL deficient mice show severe osteoporesis and complete abscence of osteoclasts as a result of lack of osteogenesis [3].
General Literature References
A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function: D.M. Anderson, et al.; Nature 390, 175 (1997) Abstract
[2] TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor: B.R. Wong, et al.; J. Exp. Med. 186, 2075 (1997) Abstract; Full Text
[3] OPGL is a key regulator of osteoclastogenesis, lymphocyte development and lymph-node organogenesis: Y.Y. Kong, et al.; Nature 397, 315 (1999) Abstract
[4] Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation: D.L. Lacey, et al.; Cell 93, 165 (1998) Abstract
[5] Tumor necrosis factor receptor family member RANK mediates osteoclast differentiation and activation induced by osteoprotegerin ligand: H. Hsu, et al.; PNAS 96, 3540 (1999) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-243 Revised 13-May-08
Monoclonal Antibody to RANKL (12A668)
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SYNONYMS anti-ODF MAb (12A668)
anti-TRANCE MAb (12A668)
anti-OPGL MAb (12A668)
anti-TNFSF 11 MAb (12A668)
anti-CD254 MAb (12A668)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-804-243-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 12A668
ISOTYPE: Mouse IgG1κ
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant mouse RANKL (aa 1-317).
SPECIFICITY: Recognizes human and mouse RANKL. Detects a band of ~35-40kDa by Western blot.
APPLICATION: Immunohistochemistry (paraffin sections (5µg/ml))
Western Blot (1-2µg/ml)
Note: Not suitable for flow cytometry.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
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Product Specific Literature References
Multiple myeloma disrupts the TRANCE/osteoprotegerin cytokine axis to trigger bone destruction and promote tumor progression: R.N. Pearse, et al.; PNAS 98, 11581 (2001) Abstract; Full Text
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
Gene expression of osteoclast differentiation factor is induced by lipopolysaccharide in mouse osteoblasts via Toll-like receptors: T. Kikuchi, et al.; J. Immunol. 166, 3574 (2001) Abstract; Full Text
Gamma-glutamyltranspeptidase stimulates receptor activator of nuclear factor-kappaB ligand expression independent of its enzymatic activity and serves as a pathological bone-resorbing factor: S. Niida, et al.; J. Biol. Chem. 279, 5752 (2004) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-830 Revised 16-Aug-07
Monoclonal Antibody to RANKL (human) (Ranky-1)
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SYNONYMS anti-ODF (human) MAb (Ranky-1)
anti-OPGL (human) MAb (Ranky-1)
anti-TRANCE (human) MAb (Ranky-1)
anti-TNFSF11 (human) MAb (Ranky-1)
anti-CD254 (human) MAb (Ranky-1)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-804-830-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Ranky-1
ISOTYPE: Rat IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human soluble RANKL extracellular domain (aa 151-316) (Prod. No. ALX-522-012).
SPECIFICITY: Recognizes human RANKL.
APPLICATION: ELISA (capture)
Immunohistochemistry (paraffin sections)
Western Blot (1:1’000)
Note: Does not work for Flow Cytometry or Immunoprecipitation.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
Product Images
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General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The molecular triad OPG/RANK/RANKL: involvement in the orchestration of pathophysiological bone remodeling: S. Theoleyre, et al.; Cytokine Growth Factor Rev. 15, 457 (2004) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-210-421 Revised 30-Oct-06
Polyclonal Antibody to RANK (ectodomain) (human)
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SYNONYMS anti-TRANCE-R (ectodomain) (human) PAb
anti-ODFR (ectodomain) (human) PAb
anti-TNFRSF 11A (ectodomain) (human) PAb
anti-CD265 (ectodomain) (human) PAb
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-210-421-C050   50 µg 195.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Contains no preservatives.
IMMUNOGEN: Recombinant human RANK.
SPECIFICITY: Recognizes human RANK. Detects a band of ~24kDa by Western blot.
APPLICATION: Flow Cytometry
Western blot (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Polyclonal Antibodies • CD Antibodies
 
 
ALX-210-396 Revised 27-Mar-08
Polyclonal Antibody to RANKL (human)
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SYNONYMS anti-ODF (human) PAb
anti-OPGL (human) PAb
anti-TRANCE (human) PAb
anti-TNFSF 11 (human) PAb
anti-CD254 (human) PAb
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-396-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From rabbit.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human RANKL.
SPECIFICITY: Recognizes human RANKL. Detects a band of ~35kDa band by Western blot.
APPLICATION: ELISA (direct and indirect: 1:2'000-1:10'000).
Flow Cytometry (1:500-1:1'000).
Western Blot (1:2'000-1:5'000 using ECL).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Polyclonal Antibodies • CD Antibodies
 
 
ALX-522-039 Revised 24-Mar-05
RANK (human):Fc (human) (recombinant)
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SYNONYMS TRANCE-R (human):Fc (human) (recombinant)
ODFR (human):Fc (human) (recombinant)
TNFRSF 11A (human):Fc (human) (recombinant)
CD265 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-522-039-C050   50 µg 360.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~55kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region of human RANK (aa 29-213) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds human and mouse RANKL.
BIOLOGICAL ACTIVITY: Inhibits human rhsRANKL-induced survival of dendritic cells and osteoclasts.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C. Once reconstituted, it is recommended to prepare appropriate aliquots and to store them at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
The receptor activator of NF-κB (RANK) is a member of the tumor necrosis factor receptor (TNF-R) family. RANK is a type I transmembrane receptor. It is 616 aa long and contains 4 cysteine-rich motifs in its extracellular domain, while its long cytoplasmic domain spans 383 aa. RANK is mainly expressed on dendritic cells. After binding its ligand RANKL, RANK interacts with TNF receptor-associated factor 1 (TRAF1), TRAF2, TRAF3, TRAF5 and TRAF6 which mediate activation of nuclear factor NF-κB and JNK. RANK-RANKL interactions promote survival of dendritic cells as well as differentiation in osteoclasts.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9Y6Q6: RANK (human) (precursor)
AfCS Signalling Gateway link A002019: RANK (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-012 Revised 28-Apr-06
RANKL, Soluble (human) (recombinant)
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SYNONYMS ODF, Soluble (human) (recombinant)
OPGL, Soluble (human) (recombinant)
TRANCE, Soluble (human) (recombinant)
TNFSF 11, Soluble (human) (recombinant)
CD254, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY RANK & RANKL
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ALX-522-012-C010   10 µg 365.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~18kDa (non-glycosylated), ~28kDa (glycosylated) (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human RANKL (aa 151-316) is fused at the N-terminus to a linker peptide (6 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
SOLUBILITY: Soluble in water.
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human and mouse RANK.
BIOLOGICAL ACTIVITY: Supports the survival of dendritic cells and osteoclasts.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
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Product Specific Literature References
Receptor activator of NF-κB and osteoprotegerin expression by human microvascular endothelial cells, regulation by inflammatory cytokines, and role in human osteoclastogenesis: P. Collin-Osdoby, et al.; J. Biol. Chem. 276, 20659 (2001) Abstract; Full Text
Functional expression of receptor activator of nuclear factor kappaB in Hodgkin disease cell lines: P. Fiumara, et al.; Blood 98, 2784 (2001) Abstract; Full Text
Suppression of IL-12 Production by Soluble CD40 Ligand: Evidence for Involvement of the p44/42 Mitogen-Activated Protein Kinase Pathway: M. Wittmann, et al.; J. Immunol. 168, 3793 (2002) Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004) Abstract
A novel in vivo role for osteoprotegerin ligand in activation of monocyte effector function and inflammatory response: D. Seshasayee, et al.; J. Biol. Chem. 279, 30202 (2004) Abstract
General Information
RANK ligand (RANKL), also called TRANCE, OPGL and ODF, is a member of the TNF family that is expressed in activated T cells, lymph nodes and in stromal cell lines. RANKL (TRANCE) interacts with its receptor RANK expressed on mature dendritic cells (DC) and mature osteoclasts, leading to the inhibition of apoptosis, probably through the upregulation of bcl-x. RANKL is, therefore, a useful tool for enhancing DC and osteoclast survival and activity. Osteoprotegerin (OPG, see Prod. No. ALX-522-007) is a potent inhibitor of RANKL.
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O014788: RANKL (human)
AfCS Signalling Gateway link A002020: RANKL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function: D.M. Anderson, et al.; Nature 390, 175 (1997) Abstract
TRANCE (tumor necrosis factor [TNF]-related activation-induced cytokine), a new TNF family member predominantly expressed in T cells, is a dendritic cell-specific survival factor: B.R. Wong, et al.; J. Exp. Med. 186, 2075 (1997) Abstract
Osteoprotegerin ligand is a cytokine that regulates osteoclast differentiation and activation</