ALEXIS Biochemicals: CD40L, Soluble (mouse) (recombinant)

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ALX-522-079

Revised 26-Aug-08

BCMA (mouse):Fc (human) (recombinant)
SYNONYMS	B Cell Maturation Protein (mouse):Fc (human) (recombinant) TNFRSF 17 (mouse):Fc (human) (recombinant) CD269 (mouse):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	BCMA

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-079-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~35kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of mouse BCMA (aa 1-46) is fused at the C-terminus to a linker peptide (2 aa) and the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds mouse and human BAFF and human APRIL.
BIOLOGICAL ACTIVITY:	Blocks the binding of mouse BAFF and human APRIL to their receptors BCMA and TACI, inhibiting BAFF and APRIL-mediated splenocyte survival.
APPLICATION:	Flow Cytometry: Detection of membrane-bound mouse BAFF and human APRIL in combination with fluorescently labelled Ab to human IgG1.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

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Product Specific Literature References

Maturation of marginal zone and follicular B cells requires B cell activating factor of the tumor necrosis factor family and is independent of B cell maturation antigen: P. Schneider, et al.; J. Exp. Med. 194, 1691 (2001) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O88472: BCMA (mouse) AfCS Signalling Gateway link A000374: BCMA (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

Related Products

ALX-203-004

Control:Fc Fusion Protein (human) (recombinant)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-204/1

Revised 06-Jun-08

pro-Bone Morphogenetic Protein 2 (human) (recombinant)
SYNONYMS	proBMP-2 (human) (recombinant)
PRODUCT LINE	Bone Metabolism
PRODUCT CATEGORY	Bone Morphogenetic Proteins [BMPs] & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-204/1-C010		10 µg	380.00 USD

Product Specification

MW:	~85.5kDa.
SOURCE/HOST:	Produced in E. coli and renatured from inclusion body material.
CONCENTRATION:	100μg/ml after reconstitution.
PURITY:	≥98% (SDS-PAGE, UV, MS)
FORMULATION:	Lyophilized from 0.1M K-HEPES, pH 7.0, containing 3% mannitol.
ENDOTOXIN CONTENT:	<0.02EU/mg protein.
RECONSTITUTION:	Reconstitute with 100μl distilled water at +4°C for 30 minutes.
BIOLOGICAL ACTIVITY:	Administration leads to ectopic bone formation in rats.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	The reconstituted solution can be diluted into other aqueous buffers and stored at +4°C for up to two weeks. Below 10μg/ml, 0.5% BSA or serum should be added.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Product Specific Literature References

Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor : F. Hillger, et al.; J. Biol. Chem. 280, 14974 (2005) Abstract; Full Text

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-205/1

Revised 17-Jun-08

Bone Morphogenetic Protein 2 (human) (recombinant)
SYNONYMS	BMP-2 (human) (recombinant)
PRODUCT LINE	Bone Metabolism
PRODUCT CATEGORY	Bone Morphogenetic Proteins [BMPs] & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-205/1-C010		10 µg	280.00 USD

Product Specification

MW:	~25.9kDa.
SOURCE/HOST:	Produced in E. coli and renatured from inclusion body material.
CONCENTRATION:	100-500μg/ml after reconstitution.
PURITY:	≥98% (SDS-PAGE, UV, MS)
FORMULATION:	Lyophilized.
ENDOTOXIN CONTENT:	<0.02EU/mg protein.
RECONSTITUTION:	Reconstitute with 20-100μl 50mM sodium acetate solution, pH 4.3.
BIOLOGICAL ACTIVITY:	Induces alkaline phosphatase expression at an EC₅₀ = 14nM in C2C12 cells.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
USE/STABILITY:	The reconstituted solution can be further diluted in sodium acetate solution and stored at +4°C for up to two weeks. Below 10μg/ml, 0.5% BSA or serum should be added.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.

Product Specific Literature References

Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor: F. Hillger, et al.; J. Biol. Chem. 280, 14974 (2005) Abstract; Full Text

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-270-313

Revised 03-Mar-05

BPIQ-I
SYNONYMS	8-[(3-Bromophenyl)amino]-3-methyl-3H-imidazo[4,5-γ]-quinazoline
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	EGFR Kinase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-313-M001		1 mg	140.00 USD

Product Specification

FORMULA:	C₁₆H₁₂BrN₅
MW:	354.2
PURITY:	≥95%
APPEARANCE:	Off white to yellow solid.
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Keep under inert gas.

Product Description

Potent and specific inhibitor of the tyrosine kinase activity of the epidermal growth factor receptor (EGFR) (IC₅₀=25 pM).

Product Specific Literature References

Tyrosine kinase inhibitors. 9. Synthesis and evaluation of fused tricyclic quinazoline analogues as ATP site inhibitors of the tyrosine kinase activity of the epidermal growth factor receptor: G.W. Rewcastle, et al.; J. Med. Chem. 39, 918 (1996) Abstract

Further Categories Containing This Product:

Tyrosine Kinase Inhibitors

ALX-270-338

Revised 03-Mar-05

BPIQ-II
SYNONYMS	8-[(3-Bromophenyl)amino]-1H-imidazo[4,5-γ]-quinazoline
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	EGFR Kinase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-338-M001		1 mg	140.00 USD

Product Specification

FORMULA:	C₁₅H₁₀BrN₅
MW:	340.2
PURITY:	≥98%
APPEARANCE:	Pale pink solid.
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Keep under inert gas.

Product Description

Potent and specific inhibitor of the tyrosine kinase activity of the epidermal growth factor receptor (EGFR) (IC₅₀=8pM).

Product Specific Literature References

Further Categories Containing This Product:

Tyrosine Kinase Inhibitors

ALX-350-246

Revised 07-Oct-08

Butein
SYNONYMS	2',3,4,4'-Tetrahydroxychalcone
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products - Protein Kinase Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-350-246-M010		10 mg	190.00 USD

Product Specification

FORMULA:	C₁₅H₁₂O₅
MW:	272.3
CAS NUMBER:	487-52-5
PURITY:	≥98% (HPLC)
APPEARANCE:	Yellow solid.
SOLUBILITY:	Soluble in DMSO (>50mg/ml) or methanol; insoluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Keep cool and dry under inert gas.
IDENTITY:	Identity determined by ¹H-NMR, ¹³C-NMR and MS.

Product Description

Plant polyphenol. Specific tyrosine kinase inhibitor. Potently inhibits the tyrosine kinase activity of the EGF receptor and p60^c-^src. Potent antioxidant and anti-inflammatory agent. Inhibits glutathione reductase and rat liver glutathione S-transferase. Activator of human deacetylase SIRT1. Inhibits aromatase, showing chemopreventive properties. Directly inhibits IKK.

Product Specific Literature References

Inhibition of 12-O-tetradecanoylphorbol-13-acetate-mediated epidermal ornithine decarboxylase induction and skin tumor promotion by new lipoxygenase inhibitors lacking protein kinase C inhibitory effects: E. Aizu, et al.; Carcinogenesis 7, 1809 (1986) Abstract
Protective effects of hydroxychalcones on free radical-induced cell damage: S. Sogawa, et al.; Biol. Pharm. Bull. 17, 251 (1994) Abstract
Cytotoxic effect of butein on human colon adenocarcinoma cell proliferation: C.C. Yit & N.P. Das; Cancer Lett. 82, 65 (1994) Abstract
Inhibitory effects of plant polyphenols on rat liver glutathione S-transferase: K. Zhang & N.P. Das; Biochem. Pharmacol. 47, 2063 (1994) Abstract
Endothelium-dependent relaxation of rat aorta by butein, a novel cyclic AMP-specific phosphodiesterase inhibitor: S.M. Yu, et al.; Eur. J. Pharmacol. 280, 69 (1995) Abstract
Butein (3,4,2',4'-tetrahydroxychalcone) ameliorates experimantal anti-glomerular basement membrane antibody-associated glomerulonephritis (3): K. Hayashi, et al.; Eur. J. Pharmacol. 316, 297 (1996) Abstract
Inhibition of glutathione reductase by plant polyphenols: K. Zhang, et al.; Biochem. Pharmacol 54, 1047 (1997) Abstract
Butein, a specific protein tyrosine kinase inhibitor: E.-B. Yang, et al.; BBRC 245, 435 (1998) Abstract
Antioxidant properties of butein isolated from Dalbergia odorifera: Z.J. Cheng, et al.; Biochim. Biophys. Acta 1392, 291 (1998) Abstract
Flavonoids inhibit cell growth and induce apoptosis in B16 melanoma 4A5 cells: K. Iwashita, et al.; Biosci. Biotechnol. Biochem. 64, 1813 (2000) Abstract
Butein, a plant polyphenol, induces apoptosis concomitant with increased caspase-3 activity, decreased Bcl-2 expression and increased Bax expression in HL-60 cells: N.Y. Kim, et al.; Pharmacol. Toxicol. 88, 261 (2001) Abstract
Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan: K.T. Howitz, et al.; Nature 425, 191 (2003) Abstract
The plant polyphenol butein inhibits testosterone-induced proliferation in breast cancer cells expressing aromatase: Y. Wang, et al.; Life Sci. 77, 39 (2005) Abstract
Butein, a tetrahydroxychalcone, inhibits nuclear factor (NF)-kappaB and NF-kappaB-regulated gene expression through direct inhibition of IkappaBalpha kinase beta on cysteine 179 residue: M.K. Pandey, et al.; J. Biol. Chem. 282, 17340 (2007) Abstract

Further Categories Containing This Product:

Tyrosine Kinase Inhibitors • Natural Products - Anti-inflammatory Agents • EGFR Kinase Inhibitors • Glutathione / Related Products • Chalcones • Phosphodiesterases / Related Products • Natural Products - Chemopreventive Agents • Glutathione S-Transferase • Sirtuins / Related Products • Natural Products - Antioxidants • IkB Kinases [IKKs] / Related Products

ALX-522-029

Revised 24-Mar-05

CD27 (human):Fc (human) (recombinant)
SYNONYMS	TNFRSF 7 (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	CD27 & CD27L [CD70] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-029-C050		50 µg	360.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
MW:	~55kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The cysteine-rich region of human CD27 (aa 1-191) is fused to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute in 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human and mouse CD70 (CD27L).
APPLICATION:	Can be used together with anti-human IgG1 PAb to detect membrane-bound human and mouse CD70.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

General Information

The CD27 receptor (CD27, Tp55) is a member of the tumor necrosis factor receptor (TNF-R) family. CD27 is a type I transmembrane receptor, it contains 3 extracellular cysteine-rich motifs. CD27 is mainly expressed on T and B lymphocytes, and its expression is increased on T cells by the engagement of the T cell receptor complex. Engagement of CD27 induces recruitment of TNF receptor-associated factor 2 (TRAF2) and TRAF3. CD27-CD70 have been reported to be involved in several different immune mechanisms such as T, NK and B lymphocytes interactions and costimulation, early thymic differentiation, autoimmune and viral diseases and apoptosis.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link P26842: CD27 (human) (precursor) AfCS Signalling Gateway link A000546: CD27 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

SENSITIVITY:	0.33ng/ml (range 1.6 to 100ng/ml)
QUANTITY:	96 wells (~80 tests).
APPLICATION:	For the quantitative determination of human sCD30 in cell culture supernatants, serum and other body fluids.
SHIPPING:	SHIPPED ON BLUE ICE
HANDLING:	Store controls at -20°C. Store rest of the kit at +4°C.

SPECIES CROSSREACTIVITY:	Human
MW:	~80kDa (SDS-PAGE).
SOURCE/HOST:	Produced in HEK 293 cells. The cysteine-rich region of human CD30 (Ki-1) (aa 1-380) is fused to the Fc portion of human IgG1 .
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY:	Binds human CD30L (CD153).
APPLICATION:	Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD30L.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

The CD30 receptor (CD30, Ki-1) is a member of the tumor necrosis factor receptor (TNF-R) family. CD30 is a type I transmembrane receptor, it contains 6 extracellular cysteine-rich motifs, is shed by the TNFα-converting enzyme and is associated with the Reed-Sternberg cells of Hodgkins disease as well as with T cells and some lymphomas. Activated CD30 recruits TNF receptor-associated factor 1, 2, 3 and 5. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION	Swiss_prot link: CD30 (human) (precursor) AfCS Signalling Gateway link: CD30 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.