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CD Proteins
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ALX-201-133 Revised 19-Feb-08
CD14 (human) (recombinant)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
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ALX-201-133-C010   10 µg 270.00 USD Add To Cart
Product Specification
MW: ~50kDa.
SOURCE/HOST: Produced in CHO cells.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ~90-95% (SDS-PAGE)
PURITY DETAIL: Affinity purified using MAb to CD14 (human) (biG 2/RoMo-1) (Prod. No. ALX-804-498).
FORMULATION: Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.00015EU/µg protein (LAL test; Chromogenix).
RECONSTITUTION: Reconstitute with 10µl distilled water. Further dilutions should be made with PBS
BIOLOGICAL ACTIVITY: Inhibits binding of LPS to CD14. Up to 20µg/ml CD14 inhibit binding of FITC-LPS (0.5µg/ml) to 6x105 CD14+ CHO transfectants (FACS).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
Both membrane-bound and soluble forms of CD14 bind to gram-negative bacteria: R.S. Jack, et al.; Eur. J. Immunol. 25, 1436 (1995) Abstract
The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms: F. Stelter, et al.; Eur. J. Biochem. 236, 457 (1996) Abstract
Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation: K. Le-Barillec, et al.; J. Clin. Invest. 103, 1039 (1999) Abstract; Full Text
General Information
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
The CD14 glycoprotein, gp 55, is present on most monocytic and macrophage like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore it is present in a soluble form in mouse serum, urine and other body fluids.
MANUFACTURER Manufactured by Biometec.
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Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-134 Revised 25-Sep-08
CD14 (mouse) (recombinant) (His)
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PRODUCT LINE Inflammation
PRODUCT CATEGORY CD14 / Related Products
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ALX-201-134-C010   10 µg 370.00 USD Add To Cart
Product Specification
MW: ~45kDa
SOURCE/HOST: Produced in CHO cells.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY DETAIL: His-tag-affinity purified.
FORMULATION: Lyophilized from PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.00015EU/µg protein (LAL test; Chromogenix).
RECONSTITUTION: Reconstitute with 10µl distilled water.
BIOLOGICAL ACTIVITY: Inhibits binding of LPS to CD14. Up to 5µg/ml mouse CD14 inhibit binding of FITC-LPS (0.5µg/ml) to mouse CD14+ CHO transfectants (FACS).
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Monocytes can phagocytose Gram-negative bacteria by a CD14-dependent mechanism: U. Grunwald, et al.; J. Immunol. 157, 4119 (1996) Abstract
General Information
The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 ist the 50kDa protein.
MANUFACTURER Manufactured by Biometec.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-128 Revised 02-Jul-08
CD26 (human) (recombinant)
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SYNONYMS DPPIV (human) (recombinant)
PRODUCT LINE Immunology
PRODUCT CATEGORY CD26 / Related Products
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ALX-201-128-1   1 Vial 257.00 USD Add To Cart
Product Specification
EC: 3.4.14.5
SOURCE/HOST: Produced in Sf9 cells.
QUANTITY: 5mU
CONCENTRATION: 40µg/ml (Pierce - BCA)
PURITY: ≥95% (SDS-PAGE, Western blot). No other protease contaminants detectable.
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 8.0, containing 5mM CaCl2, 1µM ZnCl2 and 0.05% sodium azide.
SPECIFIC ACTIVITY: ~10U/mg protein. One unit is defined as the amount of enzyme that produces 1µmol p-nitroaniline or 7-amino-4-methylcoumarin per min. at 37°C in 100mM TRIS-HCl, pH 8.5, using H-Gly-Pro-pNA or H-Gly-Pro-AMC as a substrate respectively (enzyme dilution >1:500; incubation time 10-30 min.).
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Inhibitors:
Very sensitive to diisopropyl fluorophosphate (DFP), less sensitive to other serine enzyme inhibitors such as phenylmethylsulfonyl fluoride (PMSF) (Prod. No. ALX-270-184) and diethyl 4-nitrophenylphosphate (E600). Most of the usual sulfhydryl reagents have little or no effect, nor do chelating agents.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-129 Revised 12-Apr-05
CD26 (rat) (recombinant)
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SYNONYMS DPPIV (rat) (recombinant)
PRODUCT LINE Immunology
PRODUCT CATEGORY CD26 / Related Products
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ALX-201-129-1   1 Vial 303.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf9 cells.
QUANTITY: 5mU
CONCENTRATION: 50µg/ml
PURITY: ≥95% (SDS-PAGE; Western blot)
FORMULATION: Liquid. In 20mM TRIS-HCl, pH 8.0, containing 5mM CaCl2, 1µM ZnCl2 and 0.05% sodium azide.
SPECIFIC ACTIVITY: One unit is defined as the amount of enzyme that produces 1µmol p-nitroaniline or 7-amino-4-methylcoumarin per min. at 37°C in 100mM TRIS-HCl, at pH 8.0, using H-Gly-Pro-pNA or H-Gly-Pro-AMC as a substrate respectively.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-029 Revised 24-Mar-05
CD27 (human):Fc (human) (recombinant)
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SYNONYMS TNFRSF 7 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD27 & CD27L [CD70] / Related Products
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ALX-522-029-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~55kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region of human CD27 (aa 1-191) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute in 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human and mouse CD70 (CD27L).
APPLICATION: Can be used together with anti-human IgG1 PAb to detect membrane-bound human and mouse CD70.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
The CD27 receptor (CD27, Tp55) is a member of the tumor necrosis factor receptor (TNF-R) family. CD27 is a type I transmembrane receptor, it contains 3 extracellular cysteine-rich motifs. CD27 is mainly expressed on T and B lymphocytes, and its expression is increased on T cells by the engagement of the T cell receptor complex. Engagement of CD27 induces recruitment of TNF receptor-associated factor 2 (TRAF2) and TRAF3. CD27-CD70 have been reported to be involved in several different immune mechanisms such as T, NK and B lymphocytes interactions and costimulation, early thymic differentiation, autoimmune and viral diseases and apoptosis.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P26842: CD27 (human) (precursor)
AfCS Signalling Gateway link A000546: CD27 (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
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Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-027 Revised 24-Mar-05
CD30 (human):Fc (human) (recombinant)
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SYNONYMS Ki-1 (human):Fc (human) (recombinant)
TNFRSF 8 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD30 & CD30L / Related Products
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ALX-522-027-C050   50 µg 360.00 USD Add To Cart
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Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~80kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The cysteine-rich region of human CD30 (Ki-1) (aa 1-380) is fused to the Fc portion of human IgG1 .
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human CD30L (CD153).
APPLICATION: Can be used together with anti-human IgG1 PAb to detect membrane-bound human CD30L.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
The CD30 receptor (CD30, Ki-1) is a member of the tumor necrosis factor receptor (TNF-R) family. CD30 is a type I transmembrane receptor, it contains 6 extracellular cysteine-rich motifs, is shed by the TNFα-converting enzyme and is associated with the Reed-Sternberg cells of Hodgkins disease as well as with T cells and some lymphomas. Activated CD30 recruits TNF receptor-associated factor 1, 2, 3 and 5. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION Swiss_prot link: CD30 (human) (precursor)
AfCS Signalling Gateway link: CD30 (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
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Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-028 Revised 24-Mar-06
CD30L, Soluble (human) (recombinant)
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SYNONYMS CD153, Soluble (human) (recombinant)
TNFSF 8, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD30 & CD30L / Related Products
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ALX-522-028-C010   10 µg 200.00 USD Add To Cart
ALX-522-028-3010 SuperPack 3x10 µg 410.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~35kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of recombinant human CD30L (CD153) (aa 63-234) is fused at the N-terminus to a linker peptide (11 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human CD30 (Ki-1).
APPLICATION: Can be used together with an enhancer (Prod. No. 804-034, see Set Prod. No. 850-066) to detect membrane-bound human CD30. rhsCD30L saturates its receptor at a concentration range of 12-4000ng/ml.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Specific Literature References
Selective enrichment of CD30-expressing cells within the blast region of lymphocytes from patients with primary HIV infection (PHI): P. Biswas, et al.; J. Biol. Regul. Homeost. Agents 16, 33 (2002) Abstract
Induction of Cell Cycle Arrest and Apoptosis by the Proteasome Inhibitor PS-341 in Hodgkin Disease Cell Lines Is Independent of Inhibitor of Nuclear Factor-kappaB Mutations or Activation of the CD30, CD40, and RANK Receptors: B. Zheng, et al.; Clin. Cancer Res. 10, 3207 (2004) Abstract
General Information
The CD30 ligand (CD30L, CD153) is a member of the tumor necrosis factor (TNF) family. CD30L is a type II, transmembrane protein expressed on T and B cells, monocytes/macrophages, neutrophils, megakaryocytes, erythroid precursors and eosinophils. After interaction with its receptor CD30, CD30L may induce TNF receptor-associated factor 1 (TRAF1), TRAF2, 3 and 5 recruitment. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P32971: CD30L (human)
AfCS Signalling Gateway link A000555: CD30L (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-094 Revised 27-Mar-08
CD30L, Soluble (mouse) (recombinant)
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SYNONYMS CD153, Soluble (mouse) (recombinant)
TNFSF8, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD30 & CD30L / Related Products
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ALX-522-094-C010   10 µg 365.00 USD Add To Cart
Product Specification
MW: ~42kDa (SDS-PAGE)
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse CD30L (CD153) (aa 71-239) is fused at the N-terminus to a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. The resuspended protein should be used immediately upon reconstitution to avoid loss in activity due to non-specific binding to the vial. Further dilutions should be made with medium containing 5% fetal calf serum or 0.5% BSA.
SPECIFICITY: Binds to human and mouse CD30.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
General Information
The CD30 ligand is a member of the tumor necrosis factor (TNF) family. CD30L is a type II, transmembrane protein expressed on T and B cells, monocytes/macrophages, neutrophils, megakaryocytes, erythroid precursors and eosinophils. After interaction with its receptor CD30, CD30L may induce TNF receptor-associated factor 1 (TRAF1), TRAF2, 3 and 5 recruitment. CD30-CD30L have been reported to be involved in costimulation of T cell proliferation, induction of HIV expression, negative selection and apoptosis as well as in diabetes and several allergic, viral and autoimmune diseases.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P32972: CD30L (mouse)
FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
OX40 ligand and CD30 ligand are expressed on adult but not neonatal CD4+CD3- inducer cells: evidence that IL-7 signals regulate CD30 ligand but not OX40 ligand expression: M.Y. Kim, et al.; J. Immunol. 174, 6686 (2005) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • CD Proteins
 
 
ALX-522-064 Revised 05-Apr-06
CD40 (human):COMP (human) (recombinant)
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SYNONYMS TNFRSF 5 (human):COMP (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-522-064-C010   10 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~36kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human CD40 (aa 1-193) is fused to a FLAG®-tag at the N-terminus and to human cartilage oligomeric matrix protein (COMP, aa 32-80) at the C-terminus.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human CD40L (CD154).
BIOLOGICAL ACTIVITY: Potent blocker of mouse CD40L-dependent B cell proliferation without the requirement of any crosslinking reagent (enhancer).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Description
First non-antibody blocker of CD40L signalling
Product Specific Literature References
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
General Information
COMP (Cartilage Oligomeric Matrix Protein) also designated thrombospondin 5 (TSP 5) is an oligomeric protein synthesized by chondrocytes in all type of cartilage. It belongs to the thrombospondin family of extracellular proteins. It is a pentameric molecule forming a structure similar to a bouquet of flowers. It may have storage and delivery function for hydrophobic cell-signalling molecules such as vitamin D.
COMP is a calcium-binding protein composed of 5 identical disulfide-linked subunits of 755 amino acids each with a high percentage of acidic residues (30%). The molecular weight of the homopentamer is 524 kDa, as determined by sedimentation equilibrium ultracentrifugation. Each subunit is composed of an N-terminal coiled-coil domain (unique to COMP), four epidermal growth factor (EGF)-type repeats, followed by eight calmodulin-like repeats and a globular C-terminal domain. The C-terminal domain binds to collagens I, II and IX.
Chimeras composed of proteins fused to the pentamerization domain of COMP (aa 32-75) show an increased avidity compared to the protein alone.
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Proteins • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-016 Revised 28-Apr-06
CD40 (human):Fc (human) (recombinant)
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SYNONYMS TNFRSF 5 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CD40 & CD40L [CD154] / Related Products
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ALX-522-016-C050   5