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ALX-350-084
Revised 07-Apr-08
Lavendustin C methyl ester
SYNONYMS
Compound 5 methyl ester
5-Amino-N-(2,5-dihydroxybenzyl)methyl salicylate
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Natural Products - Protein Kinase Inhibitors
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-350-084-M001
1 mg
50.00 USD
ALX-350-084-M005
5 mg
200.00 USD
Product Specification
FORMULA:
C
15
H
15
NO
5
MW:
289.3
SOURCE/HOST:
Synthetic.
PURITY:
≥97%
APPEARANCE:
Off-white solid.
SOLUBILITY:
Soluble in DMSO or methanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Protect from light.
HAZARD:
TOXIC.
Product Description
Inhibitor of EGFR tyrosine kinase with improved bioavailability over lavendustin C (Prod. No.
ALX-270-066
).
Product Specific Literature References
Synthesis and structure-activity studies of a series of [(hydroxybenzyl)amino]salicylates as inhibitors of EGF receptor- associated tyrosine kinase activity:
H. Chen, et al.; J. Med. Chem.
36
, 4094 (1993)
Abstract
Structure-activity relationships in a series of 5-[(2,5- dihydroxybenzyl)amino]salicylate inhibitors of EGF-receptor-associated tyrosine kinase: importance of additional hydrophobic aromatic interactions:
H. Chen, et al.; J. Med. Chem.
37
, 845 (1994)
Abstract
Related Products
ALX-270-066
Lavendustin C
Further Categories Containing This Product:
Tyrosine Kinase Inhibitors
•
EGFR Kinase Inhibitors
ALX-270-342
Revised 06-Dec-04
MAZ51
SYNONYMS
3-(4-Dimethylamino-naphthalen-1-yl-methylene)-1,3-dihydro-indol-2-one
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
VEGFs & VEGF-Rs Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-270-342-M002
2 mg
90.00 USD
Product Specification
FORMULA:
C
21
H
18
N
2
O
MW:
314.4
CAS NUMBER:
163655-37-6
PURITY:
≥98%
APPEARANCE:
Orange crystals.
SOLUBILITY:
Soluble in DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+4°C
HANDLING:
Keep under inert gas. Protect from light.
HAZARD:
TOXIC.
Product Description
Inhibits vascular endothelial cell growth factor-C (VEGF-C)-induced intracellular phosphorylation of VEGFR-3 (Flt-4) (IC
50
=1µM).
Product Specific Literature References
Characterization of indolinones which preferentially inhibit VEGF-C- and VEGF-D-induced activation of VEGFR-3 rather than VEGFR-2
:
V. Kirkin, et al.; Eur. J. Biochem.
268
, 5530 (2001)
Abstract
Further Categories Containing This Product:
Tyrosine Kinase Inhibitors
ALX-804-064
Revised 05-Feb-08
Monoclonal Antibody to EGF Receptor (human) (13G8)
SYNONYMS
anti-Epidermal Growth Factor Receptor (human) MAb (13G8)
anti-EGFR (human) MAb (13G8)
anti-HER-1 (human) MAb (13G8)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
EGFR Kinase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-064-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
13G8
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Synthetic peptide corresponding to aa 1165-1186 of EGF receptor (epidermal growth factor receptor).
SPECIFICITY:
Recognizes the C-terminus of human EGF receptor (aa 1165-1186). Recognition is independent of phosphorylation of Tyr
1173
.
APPLICATION:
Immunocytochemistry:
0.1-1µg/ml
Immunoprecipitation
Luminex
Western Blot:
(1µg/ml µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL:
Yes (Prod. No. ALX-840-030).
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-572
Revised 04-Jul-05
Monoclonal Antibody to EGF Receptor (human) (MGR1)
SYNONYMS
anti-EGF Receptor (human) MAb (MGR1)
anti-Epidermal Growth Factor Receptor (human) MAb (MGR1)
anti-EGFR (human) MAb (MGR1)
anti-HER-1 (human) MAb (MGR1)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
EGFR Kinase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-572-C100
100 µg
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
MGR1
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml.
FORMULATION:
Liquid. In 150mM sodium chloride, pH 7.4, containing 0.02% sodium azide.
IMMUNOGEN:
A431 vulva epidermoid carcinoma cell line.
SPECIFICITY:
Recognizes the EGF binding site of the extracellular domain of human EGFR (epidermal growth factor receptor; HER-1).
APPLICATION:
Flow Cytometry
Immunohistochemistry
(frozen sections)
Immunoprecipitation
Western Blot
: Only in not reduced condition and with a sample buffer containing 0.1% SDS. Detects a band of ~145kDa.
Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
HANDLING:
Do not freeze.
Product Specific Literature References
Characterization of a monoclonal antibody directed against the epidermal growth factor receptor binding site
:
R. Pellegrini, et al.; Cancer Immunol. Immunother.
34
, 37 (1991)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-161
Revised 05-Jun-07
Monoclonal Antibody to EGF Receptor (non-phosphorylated) (20G3)
SYNONYMS
anti-Epidermal Growth Factor Receptor (non-phosphorylated) MAb (20G3)
anti-EGFR (non-phosphorylated) MAb (20G3)
anti-HER-1 (non-phosphorylated) MAb (20G3)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
EGFR Kinase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-161-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
20G3
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min., RT).
IMMUNOGEN:
Synthetic peptide corresponding to human EGF receptor (epidermal growth factor receptor) Tyr
1173
phosporylation site.
SPECIFICITY:
Recognizes non-activated human and mouse EGF receptor (dephosphorylated at Tyr
1173
) and cross-reacts with the
1170
NAEYLRV peptide motif. Does not cross-react with the activated human and mouse EGF receptor phosphorylated at Tyr
1173
. Activated EGF receptor (phosphorylated at Tyr
1173
) can be detected in parallel using Prod. No.
ALX-804-160
,
which specifically recognizes the phosphorylated peptide motif.
APPLICATION:
Immunoprecipitation
(1-1µg/10
6
vandate treated A431 cells)
Immunocytochemistry
(0.1-1µg/ml)
Luminex
Western Blot
(1µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:
yes
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-390
Revised 05-Jun-07
Monoclonal Antibody to EGF Receptor (phosphorylated) (pSer1047) (1H9)
SYNONYMS
anti-Epidermal Growth Factor Receptor (phosphorylated) (pSer
1047
) MAb (1H9)
anti-EGFR (phosphorylated) (pSer
1047
) MAb (1H9)
anti-HER-1 (phosphorylated) (pSer
1047
) MAb (1H9)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
EGFR Kinase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-390-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
1H9
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN:
Synthetic peptide corresponding to a portion of human EGF receptor (epidermal growth factor receptor) phosphorylated at Ser
1047
.
SPECIFICITY:
Recognizes human, mouse and dog Ser
1047
-phosphorylated EGF receptor. Does not cross-react with unphosphorylated EGF receptor or other unrelated serine-phosphorylated proteins.
APPLICATION:
Immunocytochemistry
(0.1-1µg/ml)
Luminex
Western Blot
(0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL:
Included (Prod. No. ALX-840-025).
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-160
Revised 20-Sep-07
Monoclonal Antibody to EGF Receptor (phosphorylated) (Tyr1173) (9H2)
SYNONYMS
anti-Epidermal Growth Factor Receptor (phosphorylated) (Tyr
1173
) MAb (9H2)
anti-EGFR (phosphorylated) (Tyr
1173
) MAb (9H2)
anti-HER-1 (phosphorylated) (Tyr
1173
) MAb (9H2)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
EGFR Kinase Antibodies
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-160-C100
100 µg
453.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Dog
CLONE:
9H2
ISOTYPE:
Mouse IgG1
PURITY DETAIL:
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:
Lyophilized from 1ml 2x PBS containing 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:
Reconstitute with 1ml distilled water (15 min, RT).
IMMUNOGEN:
Synthetic peptide corresponding to a portion of human EGF receptor (epidermal growth factor receptor) phosphorylated at Tyr
1173
.
SPECIFICITY:
Recognizes the major autophosphorylation site N
1170
AEpYLRV of human, mouse and dog EGF receptor. Does not cross-react with the non-phosphorylated human, mouse, rat and dog EGF receptor or with unrelated Tyr-phosphorylated proteins. Non-activated EGF receptor (dephosphorylated at Tyr
1173
) can be detected in parallel using Prod. No.
ALX-804-161
that specifically recognizes the non-phosphorylated peptide motif.
APPLICATION:
Immunocytochemistry
(0.1-1µg/ml)
Immunohistochemistry
(paraffin sections, frozen sections)
Immunoprecipitation
Luminex
Western Blot
: (0.5µg/ml for HRPO/ECL detection).
Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4’000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
POSITIVE CONTROL INCLUDED:
yes
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Phospho-specific Antibodies
•
Monoclonal Antibodies
ALX-804-382
Revised 21-Feb-08
Monoclonal Antibody to Hrs and Hrs-2 (A-5)
SYNONYMS
anti-HGS MAb (A-5)
anti-Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate MAb (A-5)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Hepatocyte Growth Factor [HGF] & Receptors/Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-382-C050
50 µg
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Monkey
CLONE:
A-5
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 1
0% glycerol.
IMMUNOGEN:
Recombinant full length rat Hrs-2 (hepatocyte growth factor-regulated tyrosine kinase substrate).
SPECIFICITY:
Recognizes human, mouse, rat and monkey Hrs (HGS) and Hrs-2.
APPLICATION:
Immunohistochemistry (1:100-1:1'000)
Immunoprecipitation (2µg/ml)
Western Blot (1:2'000-1:3'000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 3 months when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Isoform-specific exocytotic protein mRNA expression in hypothalamic magnocellular neurons: regulation after osmotic challenge
:
G. Jacobsson, et al.; Neuroendocrinology
70
, 392 (1999)
Abstract
The cellular and developmental expression of hrs-2 in rat
:
S. Tsujimoto, et al.; Eur. J. Neurosci.
11
, 3047 (1999)
Abstract
Hrs-2 regulates receptor-mediated endocytosis via interactions with Eps15
:
A.J. Bean, et al.; J. Biol. Chem.
275
, 15271 (2000)
Abstract
;
Full Text
Distinct protein domains are responsible for the interaction of Hrs-2 with SNAP-25. The role of Hrs-2 in 7 S complex formation
:
S. Tsujimoto & A.J. Bean; J. Biol. Chem.
275
, 2938 (2000)
Abstract
;
Full Text
SNAP-25-associated Hrs-2 protein colocalizes with AQP2 in rat kidney collecting duct principal cells:
A. Shukla, et al.; Am. J. Physiol. Renal. Physiol.
281
, F546 (2001)
Abstract
;
Full Text
CART: an Hrs/actinin-4/BERP/myosin V protein complex required for efficient receptor recycling:
Q. Yan, et al.; Mol. Biol. Cell
16
, 2470 (2005)
Abstract
;
Full Text
Hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) interacts with PELP1 and activates MAPK:
S.K. Rayala, et al.; J. Biol. Chem.
281
, 4395 (2006)
Abstract
;
Full Text
General Information
Hrs/Hgs (hepatocyte growth factor-regulated tyrosine kinase substrate) is a tyrosine-phosphorylated member of a protein family characterized by a conserved 3´-phosphoinositide-interacting RING zinc finger domain, the so called FYVE finger. A variant of Hrs, Hrs-2 is an ATPase that has been shown to play a regulatory role in the docking and/or fusion of synaptic vesicles to plasma membranes through a calcium-regulated interaction with SNAP-25. Hrs-2 physically interacts with Eps15, a protein required for receptor-mediated endocytosis. The Hrs-2/Eps15 interaction is calcium dependent, inhibited by SNAP-25 and alpha-adaptin, and results in the inhibition of receptor-mediated endocytosis. Immunoelectron microscopy reveals Hrs-2 localization on the limiting membrane of multivesicular bodies, organelles in the endosomal pathway. Recent data suggest that Hrs-2 acts to provide communication between endo- and exocytic processes.
Further Categories Containing This Product:
Vesicles & Membrane Trafficking Other Products
•
Autophagy-related Proteins [APG; ATG]/Related Products
•
Monoclonal Antibodies
ALX-804-857
Revised 18-Jun-08
Monoclonal Antibody to ILEI (Delphy-1)
SYNONYMS
anti-Interleukin-like EMT Inducer MAb (Delphy-1)
anti-FAM3C MAb (Delphy-1)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Chemokines & Cytokines Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-857-C100
100 µg
360.00 USD
ALX-804-857B-C100
Biotin
100 µg
395.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
Delphy-1
ISOTYPE:
Mouse IgG2a
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human ILEI (aa 42-227).
SPECIFICITY:
Recognizes human and mouse ILEI.
APPLICATION:
ELISA
Immunoprecipitation (1:100)
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
USE/STABILITY:
Stable for at least 1 year after receipt when stored as recommended.
HANDLING:
For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep ATTO, Biotin- and FITC-conjugated formats at +4°C. Do not freeze. Protect from light.
General Information
In 2006, T. Waerner, et al. characterized ILEI as a key player in both epithelial to mesenchymal transition and late-stage carcinoma progression. EMT is a process that involves changes in gene expression, cytoskeleton organization, cell adhesion, and extracellular matrix composition. This process is important during tissue formation and organogenesis, but also during cancer progression, metastasis and chronic inflammation. ILEI is overexpressed and/or altered in intracellular localization in multiple human tumors, an event strongly correlated to invasion/EMT, metastasis formation, and survival in human colon and breast cancer.
MANUFACTURER
Manufactured by Apotech Corporation.
General Literature References
Cloning, expression, and initial characterization of a novel cytokine-like gene family:
Y. Zhu, et al.; Genomics
80
, 144 (2002)
Abstract
ILEI: a cytokine essential for EMT, tumor formation, and late events in metastasis in epithelial cells:
T. Waerner, et al.; Cancer Cell
10
, 227 (2006)
Abstract
Further Categories Containing This Product:
Cancer Other Products
•
Transforming Growth Factor beta [TGFbeta] & Receptors/Related Products
•
Monoclonal Antibodies
ALX-804-858
Revised 18-Jun-08
Monoclonal Antibody to ILEI (Delphy-2)
SYNONYMS
anti-Interleukin-like EMT Inducer MAb (Delphy-2)
anti-FAM3C MAb (Delphy-2)
PRODUCT LINE
Chemokines & Cytokines
PRODUCT CATEGORY
Chemokines & Cytokines Other Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-858-C100
100 µg
360.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE:
Delphy-2
ISOTYPE:
Mouse IgM
SOURCE/HOST:
Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
PURITY DETAIL:
Protein G-affinity purified.
FORMULATION:
Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:
Recombinant human ILEI (aa 42-227).
SPECIFICITY:
Recognizes human and mouse ILEI.
APPLICATION:
