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Chemokines & Cytokines
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ALX-201-350 Revised 01-Oct-08
IL-33 (human) (recombinant) (His)
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SYNONYMS Interleukin-33 (human) (recombinant) (His)
NF-HEV (human) (recombinant) (His)
IL-1F11 (human) (recombinant) (His)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-350-C010   10 µg 295.00 USD Add To Cart
ALX-201-350-C050   50 µg 795.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Recombinant human IL-33 (aa 112-270) is fused at the C-terminus to a His-tag.
CONCENTRATION: 1mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing
150mM sodium chloride.
ENDOTOXIN CONTENT: <1.0EU/µg protein (LAL-test).
SPECIFICITY: Binds to human ST2.
BIOLOGICAL ACTIVITY: Activates ST2-dependent NF-κB pathway.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q2YEJ5: Interleukin-33 (human) (recombinant)
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Interleukin-33 [IL-33] & Receptors / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-361 Revised 01-Oct-08
IL-33 (mouse) (recombinant) (His)
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SYNONYMS Interleukin-33 (mouse) (recombinant) (His)
NF-HEV (mouse) (recombinant) (His)
IL-1F11 (mouse) (recombinant) (His)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-361-C010   10 µg 295.00 USD Add To Cart
ALX-201-361-C050   50 µg 795.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Recombinant mouse IL-33 (aa 109-266) is fused at the C-terminus to a His-tag.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing
150mM sodium chloride.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SPECIFICITY: Binds to human ST2.
BIOLOGICAL ACTIVITY: Activates ST2-dependent NF-κB pathway.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q2YEJ4: Interleukin-33 (mouse) (recombinant)
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Interleukin-33 [IL-33] & Receptors / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-363 Revised 01-Oct-08
IL-33 (human) (recombinant)
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SYNONYMS Interleukin-33 (human) (recombinant)
NF-HEV (human) (recombinant)
IL-1F11 (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-363-C010   10 µg 295.00 USD Add To Cart
ALX-201-363-C050   50 µg 795.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Recombinant human IL-33 (aa 112-270) is fused at the C-terminus to a FLAG®-tag.
CONCENTRATION:  
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS, pH 7.2.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SPECIFICITY: Binds to human ST2.
BIOLOGICAL ACTIVITY: Activates ST2-dependent NF-κB pathway.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q2YEJ5: Interleukin-33 (human) (recombinant)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Interleukin-33 [IL-33] & Receptors / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-374 Revised 18-Mar-08
IL-17A (human):Fc (human) (recombinant)
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SYNONYMS Interleukin-17A (human):Fc (human) (recombinant)
CTLA-8 (human):Fc (human) (recombinant)
Cytotoxic T-lymphocyte-associated Antigen 8 (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-374-C010   10 µg 140.00 USD Add To Cart
ALX-201-374-C050   50 µg 380.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. The signal peptide and the sequence of human IL-17A (interleukin-17A) (aa 1-155) are fused at the C-terminus to the Fc portion of human IgG1.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q16552: Interleukin-17A (human) [Precursor]
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-419 Revised 14-Oct-08
Epstein-Barr Virus-induced Gene 3 (human):Fc (human) (recombinant)
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SYNONYMS EBI3 (human):Fc (human) (recombinant)
EBV-induced Gene 3 Protein (human):Fc (human) (recombinant)
IL-27 Subunit β (human):Fc (human) (recombinant)
Interleukin-27 Subunit β (human):Fc (human) (recombinant)
IL-27B (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-419-C010   10 µg 280.00 USD Add To Cart
ALX-201-419-C050   50 µg 680.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Full-length human Epstein-Barr Virus-induced Gene 3 (aa 1-229) is fused at the C-terminus to the Fc portion of human IgG1.
CONCENTRATION:  
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2μm-filtered solution in PBS.
ENDOTOXIN CONTENT: <1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
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General Information
EBI3 (Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein; IL-27B; IL-27 subunit β) and IL-12A (IL-12 subunit p35; CLMF p35) form the heterodimeric cytokine IL-35 (interleukin-35) involved in T cell regulation.
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q14213: Epstein-Barr Virus-induced Gene 3 (human) 
MANUFACTURER Manufactured by AdipoGen, Inc.
General Literature References
Epstein-Barr virus-induced gene 3 and the p35 subunit of interleukin 12 form a novel heterodimeric hematopoietin: O. Devergne, et al.; PNAS 94, 12041 (1997) Abstract; Full Text
IL-35 is a novel cytokine with therapeutic effects against collagen-induced arthritis through the expansion of regulatory T cells and suppression of Th17 cells: W. Niedbala, et al.; Eur. J. Immunol. 37, 3021 (2007) Abstract; Full Text
The inhibitory cytokine IL-35 contributes to regulatory T-cell function: L.W. Collison, et al.; Nature 450, 566 (2007) Abstract
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-520-001 Revised 01-Oct-08
IL-1β (human) (recombinant) (cell culture grade)
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SYNONYMS Interleukin-1β (human) (recombinant) (cell culture grade)
IL-1F2 (human) (recombinant) (cell culture grade)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-520-001-C010   10 µg 297.00 USD Add To Cart
Product Specification
MW: ~17kDa.
CAS NUMBER: 97599-21-8
MERCK INDEX: 14: 4993
SOURCE/HOST: Produced in E. coli.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Lyophilized. Salt-free.
ENDOTOXIN CONTENT: <1EU/µg protein.
RECONSTITUTION: Reconstitute with water, PBS or aqueous buffer to a concentration of 100µg/ml. For further dilution carrier protein (~1%) should be added to avoid loss of bioactivity.
BIOLOGICAL ACTIVITY: Exerts in vitro biological activity in the range of 0.1-10ng/ml.
SPECIFIC ACTIVITY: ≥1x107U/mg protein. Determined by proliferation assay using human TF-1 (T. Kitamura, et al.; Int. Immunol. 3, 571 (1991)). The ED50 for this effect is typically 0.2-2ng/ml. Results were compared with the international IL-1 reference standard preparation (WHO 86/680, NIBSC).
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Potent immunomodulator. Mediates a wide range of immune and inflammatory responses including the activation of B and T cells.
Further Categories Containing This Product:
Interleukin-1 [IL-1] & Receptors / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-056 Revised 18-Sep-07
IL-1β, Soluble (human) (recombinant)
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SYNONYMS Interleukin-1β, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-056-C010   10 µg 200.00 USD Add To Cart
ALX-522-056-3010 SuperPack 3x10 µg 410.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: Produced in E. coli. Human IL-1β (interleukin-1β) (aa 117-270) is fused to a linker peptide (10 aa) and an N-terminal FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water.
SPECIFICITY: Binds to human IL-1R (interleukin-1 receptor).
BIOLOGICAL ACTIVITY: Activates the NF-κB pathway.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
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Product Specific Literature References
Insulin-secreting beta-cell dysfunction induced by human lipoproteins: M.E. Roehrich, et al.; J. Biol. Chem. 278, 18368 (2003) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P01584: Interleukin-1β (human) (precursor)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Human interleukin 1: I. Gery & J.A. Schmidt; Meth. Enzymol. 116, 456 (1985) Abstract
Interleukin 1: an immunological perspective: S.K. Durum, et al.; Annu. Rev. Immunol. 3, 263 (1985) Abstract
The in vivo effects of interleukin 1. I. Bone marrow cells are induced to cycle after administration of interleukin 1: R. Neta, et al.; J. Immunol. 139, 1861 (1987) Abstract
Interleukin 1 or tumor necrosis factor-alpha: which is the real target in rheumatoid arthritis?: J.M. Dayer; J. Rheumatol. Suppl. 65, 10 (2002) Abstract
Further Categories Containing This Product:
Interleukin-1 [IL-1] & Receptors / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-098 Revised 04-Nov-08
IL-33 (human) (recombinant)
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SYNONYMS Interleukin-33 (human) (recombinant)
NF-HEV (human) (recombinant)
IL-1F11 (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-098-C010   10 µg 190.00 USD Add To Cart
ALX-522-098-3010 SuperPack 3x10 µg 410.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~18kDa
SOURCE/HOST: Produced in E. coli. Untagged human IL-33 (aa 112-270).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human ST2.
BIOLOGICAL ACTIVITY: Activates human ST2-dependent NF-κB pathway. Does not activate mouse ST2-dependent NF-κB.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated nuclear factor in vivo: V. Carriere et al.; PNAS 104, 282 (2007) Abstract
Human basophils and eosinophils are the direct target leukocytes of the novel IL-1-family member IL-33: T. Pecaric-Petkovic et al.; Blood Epub ahead of print, (2008) Abstract
General Information
IL-33 is a new member of the IL-1 family. It is involved in T helper type 2 response via the activation of its receptor ST2 (IL-1RL1) driving the production of Th2-associated cytokines. The stimulation of ST2 by IL-33 induces the NF-κB and MAPK pathways. The human IL-33 protein is expressed with a prodomain (aa 1-111) which is cleaved by caspase-1 to produce the 18kDa mature protein (112-270).
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q2YEJ5: Interleukin-33 (human)
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines: J. Schmitz, et al.; Immunity 23, 479 (2005) Abstract
Related Products
Further Categories Containing This Product:
Interleukin-33 [IL-33] & Receptors / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-522-101 Revised 01-Oct-08
IL-33 (mouse) (recombinant)
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SYNONYMS Interleukin-33 (mouse) (recombinant)
NF-HEV (mouse) (recombinant)
IL-1F11 (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY Interleukins
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-101-C010   10 µg 190.00 USD Add To Cart
ALX-522-101-3010 SuperPack 3x10 µg 410.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~18kDa
SOURCE/HOST: Produced in E. coli. Untagged mouse IL-33 (aa 109-266).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to mouse and human ST2.
BIOLOGICAL ACTIVITY: Activates mouse and human ST2-dependent NF-κB pathway. Induces IL-6 production in P815 mastocytoma cells and in bone marrow derived mast cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature Refere