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CX3C Chemokines & Receptors / Related Products
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ALX-201-388 Revised 08-Oct-08
CX3CL1, Soluble  (human) (recombinant)
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SYNONYMS Fractalkine, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CX3C Chemokines & Receptors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-388-C010   10 µg 170.00 USD Add To Cart
ALX-201-388-C050   50 µg 590.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in HEK 293 cells. Signal peptide and extracellular domain of human CX3CL1 (fractalkine) (aa 1-339) is fused at the C-terminus to a FLAG®-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
BIOLOGICAL ACTIVITY: Chemotaxis effect of soluble recombinant human CX3CL1 (fractalkine) in THP-1 cells (see Figure 2).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P78423: Fractalkine (human) [Precursor]

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-804-472 Revised 10-Feb-05
Monoclonal Antibody to CX3CL1 (human) (20/20)
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SYNONYMS anti-Fractalkine (human) MAb (20/20)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CX3C Chemokines & Receptors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-472-C250   250 µg 540.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 20/20
ISOTYPE: Rat IgG
PURITY DETAIL: Purified by protein G affinity chromatography.
FORMULATION: Liquid. In PBS, pH 7.4.
IMMUNOGEN: Recombinant human CX3CL1 (fractalkine).
SPECIFICITY: Recognizes human CX3CL1.
APPLICATION: ELISA (detection (1-2µg/ml))
Upon conjugation with a suitable label this antibody can be used with primary antibody (Prod. No. ALX-804-471, MAb to CX3CL1 (human) (28/31) at 20µg/ml) to detect human fractalkine.
Detection Limit: ~0.5ng/ml.
Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-471 Revised 05-Aug-05
Monoclonal Antibody to CX3CL1 (human) (28/31)
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SYNONYMS anti-Fractalkine (human) MAb (28/31)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CX3C Chemokines & Receptors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-471-C250   250 µg 540.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 28/31
ISOTYPE: Rat IgG
PURITY DETAIL: Purified by protein G affinity chromatography.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human CX3CL1 (fractalkine).
SPECIFICITY: Recognizes human CX3CL1.
APPLICATION: ELISA (capture (20µg/ml))
Use microtiter plates coated with this antibody in 0.05M carbonate/bicarbonate buffer, pH 9.6, in combination with secondary antibody (Prod. No. ALX-804-472, MAb to CX3CL1 (human) (20/20) at 1-2µg/ml to detect human fractalkine.
Detection Limit: ~1ng/ml.
Substrate: 0.26mg/ml tetramethyl benzydine and 0.01% hydrogen peroxide stabilized in 0.05M citrate buffer, pH 3.8.
Western Blot (2-4µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-210-476 Revised 26-Oct-07
Polyclonal Antibody to CX3CL1 (human)
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SYNONYMS anti-Fractalkine (human) PAb
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY CX3C Chemokines & Receptors / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-476-C100   100 µg 350.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
SOURCE/HOST: From guinea pig.
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Purified IgG.
FORMULATION: Liquid. In PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN: Recombinant human CX3CL1 (fractalkine).
SPECIFICITY: Recognizes human CX3CL1.
APPLICATION: ELISA (direct and indirect: 1:2’000-1:5’000)
Western Blot (1:2’000-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 1-2 weeks when stored at +4°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
MANUFACTURER Manufactured by AdipoGen, Inc.
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
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