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ALX-201-243
Revised 26-Apr-06
ETS1 (human) (recombinant)
SYNONYMS
c-ets-1 Protein (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Transcription Factors Other Products
Ordering Information
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ALX-201-243-C050
50 µg
210.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
.
CONCENTRATION:
2mg/ml
PURITY:
≥90% (SDS-PAGE)
APPEARANCE:
Liquid. In 50mM TRIS-KOH, pH 7.0, containing 6mM magnesium dichloride, 50mM potassium chloride, 5µM zinc dichloride, 2mM DTT and 0.1% NP40.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
USE/STABILITY:
For consistent results use a fresh aliquot for each experiment.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
General Information
The Ets family of transcription factors is known to control the expression of genes critical for cellular proliferation, differentiation, and transformation. They activate transcription by interaction with purine-rich sequences in promoter/enhancer regions of various genes. Differential expression of Ets genes is particularly associated with embryonic development of the central nervous system. Previous studies have implicated Ets transcription factors in apoptosis; however, exact mechanisms remain unclear. The mammalian Ets1 gene encodes a set of nuclear phosphoproteins ranging in size from 42 to 54kD.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-244
Revised 27-Mar-07
Endonuclease G (human) (recombinant)
SYNONYMS
EndoG (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
HtrA2 / Omi, ARTS, EndoG / Related Products
Ordering Information
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ALX-201-244-C020
20 µg
190.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Recombinant human endonuclease G is fused to a His-tag at the C-terminus.
CONCENTRATION:
0.4mg/ml
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 8.0, containing 500mM sodium chloride, 0.05% Tween 20, 5mM β-mercaptoethanol, 500mM imidazole and 20% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
DNA Endonucleases / Related Products
•
Recombinant Proteins / Fusion Proteins
ALX-201-245
Revised 27-Apr-06
CED-4 (C. elegans) (recombinant)
SYNONYMS
Cell Death Protein 4 (
C. elegans
) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
Apoptosome Other Products
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ALX-201-245-C020
20 µg
170.00 USD
Product Specification
CONCENTRATION:
0.8mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM HEPES, pH 7.9, containing 100mM sodium chloride, 1mM DTT, 2mM EDTA and 20% glycerol.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stable for 1-2 weeks when stored at -20°C.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-246
Revised 24-Apr-08
Matriptase (human) (recombinant)
SYNONYMS
MT-SP1 (human) (recombinant)
Membrane-type Serine Protease 1 (human) (recombinant)
ST14 (human) (recombinant)
Suppression of Tumorigenicity Protein 14 (human) (recombinant)
TADG-15 (human) (recombinant)
Tumor-associated Differentially Expressed Gene 15 Protein (human) (recombinant)
SNC19 (human) (recombinant)
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
Proteases Other Products
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ALX-201-246-U125
125 U
360.00 USD
Product Specification
MW:
~25kDa
SOURCE/HOST:
Recombinant human matriptase (aa 596-855) fused at the N-terminus to a His-tag.
CONCENTRATION:
100nM
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 100mM TRIS, pH 9.0, containing 200mM sodium chloride, 1mM β-mercaptoethanol and 10% glycerol.
ACTIVITY:
Trypsin-like serine protease.
SPECIFIC ACTIVITY:
~2.5U/µl. One unit is defined as the amount of enzyme that releases 1nmol of AMC per min. at 37°C in the presence of 50µM of Boc-Gln-Ala-Arg-AMC.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stable for at least 6 months when stored at -20°C. Do not store diluted solutions.
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
Reverse biochemistry: use of macromolecular protease inhibitors to dissect complex biological processes and identify a membrane-type serine protease in epithelial cancer and normal tissue:
T. Takeuchi, et al.; PNAS
96
, 11054 (1999)
Abstract
;
Full Text
Inhibition of human matriptase by eglin c variants:
A. Désilets, et al.; FEBS Lett.
580
, 2227 (2006)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Recommended Assay Buffer:
100mM TRIS, pH 9.5, containing 5mg/ml BSA.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
•
Cancer Other Products
•
Cytoskeleton & Extracellular Matrix Other Products
ALX-201-247
Revised 18-Mar-08
NS3-NS4A (HCV) (recombinant) (His)
SYNONYMS
Nonstructural Protein 3-NS4A (HCV) (recombinant) (His)
Hepacivirin-NS4A (HCV) (recombinant) (His)
PRODUCT LINE
Viral Signalling
PRODUCT CATEGORY
Hepatitis Viruses / Related Products
Ordering Information
Product Numbers:
Format:
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ALX-201-247-C100
100 µg
210.00 USD
Product Specification
MW:
~68.7kDa (NS3 subunit); ~6.2kDa (NS4A subunit)
EC:
3.4.21.98
SOURCE/HOST:
Produced in
E. coli
. Recombinant hepatitis C virus (HCV) NS3-NS4A serine protease complex is fused to a His-tag.
CONCENTRATION:
379ng/μl (5µM)
PURITY:
≥85% (SDS-PAGE)
FORMULATION:
Liquid. In 25mM HEPES, pH 7.6, containing 1mM EDTA, 3mM DTT, 0.5M sodium chloride, 0.2% Triton X-100 and 20% glycerol.
BIOLOGICAL ACTIVITY:
IC
50
(lot-specific)
APPLICATION:
Suitable for screenings.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-249
Revised 18-Mar-08
FEN-1 (human) (recombinant)
SYNONYMS
Flap Structure-specific Endonuclease 1 (human) (recombinant)
MF1 (human) (recombinant)
Maturation Factor 1 (human) (recombinant)
DNase IV (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
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ALX-201-249-C010
10 µg
260.00 USD
Product Specification
MW:
~42kDa
SOURCE/HOST:
Produced in
E. coli
.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 1mM DTT, 1mM EDTA and 0.02% Nonidet P40.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stable for at least 4 weeks when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Flap endonuclease 1: a novel tumour suppresser protein:
G. Henneke, et al.; TIBS
28
, 384 (2003)
Abstract
The Fen1 extrahelical 3’-flap pocket is conserved from archaea to human and regulates DNA substrate specificity:
E. Friedrich-Heineken and U. Hubscher; Nucl. Acids Res.
32
, 2520 (2004)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P39748:
FEN-1 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-250
Revised 18-Mar-08
PARP-1 (human) (recombinant) (high purity) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
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ALX-201-250-C010
10 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length PARP-1 is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY:
≥1’200U/mg protein.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1:
P.O. Hassa, et al.; J. Biol. Chem.
278
, 45145 (2003)
Abstract
;
Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription:
P.O. Hassa, et al.; J. Biol. Chem.
280
, 40450 (2005)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-251
Revised 18-Mar-08
PARP-2 (mouse) (recombinant) (high purity) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-2 (mouse) (recombinant) (high purity) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-2
Ordering Information
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ALX-201-251-C010
10 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Mouse full-length PARP-2 is fused to a His-tag.
CONCENTRATION:
1mg/ml.
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link O88554:
PARP-2 (mouse)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-252
Revised 18-Mar-08
PARP-3 (human) (recombinant) (high purity) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-3 (human) (recombinant) (high purity) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-3
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ALX-201-252-C010
10 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length PARP-3 is fused to a His-tag.
CONCENTRATION:
1mg/ml.
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link Q9Y6F1:
PARP-3 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-253
Revised 18-Mar-08
PARP-1b (human) (recombinant) (active) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1b (human) (recombinant) (active) (His)
short-form PARP-1 (human) (recombinant) (active) (His)
sPARP-1 (human) (recombinant) (active) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
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ALX-201-253-C020
20 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human PARP-1b (short-form PARP1; sPARP-1) is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Characterization of sPARP-1. An alternative product of PARP-1 gene with poly(ADP-ribose) polymerase activity independent of DNA strand breaks:
F.R. Sallmann, et al.; J. Biol. Chem.
275
, 15504 (2000)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
General Literature References
Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going?:
P.O. Hassa, et al.; Microbiol. Mol. Biol. Rev.
70
, 789 (2006)
Abstract
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-254
Revised 18-Mar-08
PARP-1 (E988K mutant) (human) (recombinant) (Control)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (E988K mutant) (human) (recombinant) (Control)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
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ALX-201-254-C010
10 µg
180.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length inactive mutant E988K of PARP-1 is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY:
0.5% of wild type PARP-1.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function:
P.O. Hassa, et al.; J. Biol. Chem.
276
, 45588 (2001)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-255
Revised 18-Mar-08
PARP-1 (BRCT Domain) (human) (recombinant) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (BRCT Domain) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
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ALX-201-255-C020
20 µg
290.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Human PARP-1 (BRCT domain) (aa 385-524) is fused to a His-tag.
CONCENTRATION:
1mg/ml
PURITY:
≥99% (SDS-PAGE)
PURITY DETAIL:
Affinity purified on Ni-beads.
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 8, containing 200mM sodium chloride, 60mM imidazole, 0.5mM PMSF and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
