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ALX-805-038 Revised 23-Jul-08
Monoclonal Antibody to Fas (human) (2R2)
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SYNONYMS anti-CD95 (human) MAb (2R2)
anti-APO-1 (human) MAb (2R2)
anti-TNFRSF 6 MAb (human) (2R2)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-805-038-C100   100 µg 340.00 USD Add To Cart
ALX-805-038B-C100 Biotin 100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 2R2
ISOTYPE: Mouse IgG3
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE).
PURITY DETAIL: Protein A-affinity purified.
FORMULATION: 2R2: Liquid. In PBS containing 1% BSA. Contains no preservatives.
2R2-Biotin: Liquid. In PBS.
IMMUNOGEN: Mouse L cells stably overexpressing human Fas (CD95; APO-1).
SPECIFICITY: Recognizes human Fas.
APPLICATION: 2R2:
Flow Cytometry
Functional Application (induces apoptosis - no cross-linking required).
- Can be used in DISC (death-inducing signalling complex assays): Co-immunoprecipitates FADD and caspase-8 after receptor activation.

2R2-Biotin:
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (frozen sections, paraffin sections)
- Recommended working dilutions are 1:200-1:1000.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
Product Images
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Product Specific Literature References
Differential regulation and ATP requirement for caspase-8 and caspase-3 activation during CD95- and anticancer drug-induced apoptosis: D. Ferrari, et al.; J. Exp. Med. 188, 979 (1998) Abstract; Full Text
Anticancer drugs induce caspase-8/FLICE activation and apoptosis in the absence of CD95 receptor/ligand interaction: S. Wesselborg, et al.; Blood 93, 3053 (1999) Abstract; Full Text
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt, et al.; Eur. J. Immunol. 30, 1769 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION IHC (PS) Antigen Retrieval: we recommend using a citric acid solution together with microwave retrieval (5 x 5 minutes at high intensity).
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-805-022 Revised 29-Jul-08
Monoclonal Antibody to Fas (human) (APO-1-1)
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SYNONYMS anti-CD95 (human) MAb (APO-1-1)
anti-APO-1 (human) MAb (APO-1-1)
anti-TNFRSF 6 (human) MAb (APO-1-1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-805-022-C100 Purified 100 µg 259.00 USD Add To Cart
ALX-805-022B-T100 Biotin 100 tests 308.00 USD Add To Cart
ALX-805-022F-T100 FITC 100 tests 308.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: APO-1-1
ISOTYPE: Mouse IgG1
PURITY: ≥95% (SDS-PAGE).
FORMULATION: APO-1-1-Purified: Liquid. 0.1mg/ml in PBS.
APO-1-1-Biotin and -FITC: Liquid. In 1ml 50mM TRIS, pH 7.4, containing 100mM sodium chloride, 1% BSA and 0.02% sodium azide.
IMMUNOGEN: Human Fas (CD95; APO-1).
SPECIFICITY: Recognizes human Fas.
APPLICATION: Immunohistochemistry (frozen sections):
APO-1-1 (ALX-805-022; IgG1) is an isotype-switch variant of the original clone APO-1-3 (ALX-805-020). Since undesired side reactions in immunohistochemistry are minimal for IgG1 antibodies, APO-1-1 is especially useful for immunohistology on cryostat sections and on cytospin preparations [1].
Note: Not suitable for stainings on paraffin sections.

Flow Cytometry:
APO-1-1-Purified: 1-2µg/ml
APO-1-1-Biotin and -FITC: 1:20

Functional Application:
Induces apoptosis upon cross-linking [2].

Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening aliquot contents and store at -20°C.
Product Specific Literature References
[1] Constitutive and induced expression of APO-1, a new member of the nerve growth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells: F. Leithauser, et al.; Lab. Invest. 69, 415 (1993) Abstract
[2] Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L): D.C. Huang, et al.; PNAS 96, 14871 (1999) Abstract
[3] Inhibition of death receptor-mediated gene induction by a cycloheximide-sensitive factor occurs at the level of or upstream of Fas-associated death domain protein (FADD): H. Wajant, et al.; J. Biol. Chem. 275, 24357 (2000) Abstract; Full Text
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-805-020 Revised 25-Apr-07
Monoclonal Antibody to Fas (human) (APO-1-3)
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SYNONYMS anti-CD95 (human) MAb (APO-1-3)
anti-APO-1 (human) MAb (APO-1-3)
anti-TNFRSF 6 (human) MAb (APO-1-3)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-805-020-C100 Purified 100 µg 295.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: APO-1-3
ISOTYPE: Mouse IgG3
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. 1mg/ml in PBS. Contains no preservatives.
IMMUNOGEN: Human recombinant Fas (CD95; APO-1).
SPECIFICITY: Recognizes human Fas.
APPLICATION: Flow Cytometry (suitable as a primary antibody)
Immunoprecipitation
Western Blot
Functional Application: Induces apoptosis with or without cross-linking (Protein A), depending on cell type.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Monoclonal antibody-mediated tumor regression by induction of apoptosis: B.C. Trauth, et al.; Science 245, 301 (1989) Abstract
Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia: K.M. Debatin, et al.; Lancet 335, 497 (1990) Abstract
Induction of apoptosis by monoclonal antibody anti-APO-1 class switch variants is dependent on cross-linking of APO-1 cell surface antigens: J. Dhein, et al.; J. Immunol. 149, 3166 (1992) Abstract
APO-1-induced apoptosis of leukemia cells from patients with adult T-cell leukemia: K.M. Debatin, et al.; Blood 81, 2972 (1993) Abstract
Apoptotic cell death induced by a mouse-human anti-APO-1 chimeric antibody leads to tumor regression: L.R. Coney, et al.; Int. J. Cancer 58, 562 (1994) Abstract
Activation induces sensitivity toward APO-1 (CD95)-mediated apoptosis in human B cells: P.T. Daniel & P.H. Krammer; J. Immunol. 152, 5624 (1994) Abstract
APO-1 (CD95) mediated apoptosis in human T-ALL engrafted in SCID mice: K.M. Lücking-Famira, et al.; Leukemia 8, 1825 (1994) Abstract
The apoptosis-1/Fas protein in human systemic lupus erythematosus: E. Mysler, et al.; J. Clin. Invest. 93, 1029 (1994) Abstract
Divergent signalling via APO-1/Fas and the TNF receptor, two homologous molecules involved in physiological cell death: K. Schulze-Osthoff, et al.; EMBO J. 13, 4587 (1994) Abstract
Cell nucleus and DNA fragmentation are not required for apoptosis: K. Schulze-Osthoff, et al.; J. Cell Biol. 127, 15 (1994) Abstract
Inhibition of apoptosis in T cells expressing human T cell leukemia virus type I Tax: K.F. Copeland, et al.; AIDS Res. Hum. Retroviruses 10, 1259 (1994) Abstract
Resistance to APO-1 (CD95) induced apoptosis in T-ALL is determined by a BCL-2 independent anti-apoptotic program: K.M. Debatin & P.H. Krammer; Leukemia 9, 815 (1995) Abstract
Cytotoxicity-dependent APO-1 (Fas/CD95)-associated proteins form a death-inducing signaling complex (DISC) with the receptor: F.C. Kischkel, et al.; EMBO J. 14, 5579 (1995) Abstract
CD40 ligation induces Apo-1/Fas expression on human B lymphocytes and facilitates apoptosis through the Apo-1/Fas pathway: E.J. Schattner, et al.; J. Exp. Med. 182, 1557 (1995) Abstract
Sensitization of T cells to CD95-mediated apoptosis by HIV-1 Tat and gp120: M.O. Westendorp, et al.; Nature 375, 497 (1995) Abstract
Autocrine T-cell suicide mediated by APO-1/(Fas/CD95): J. Dhein, et al.; Nature 373, 438 (1995) Abstract
The medical significance of physiological cell death: G. Häcker & D.L. Vaux; Med. Res. Rev. 15, 299 (1995) Abstract
Involvement of the CD95 (APO-1/FAS) receptor/ligand system in drug-induced apoptosis in leukemia cells: C. Friesen, et al.; Nat. Med. 2, 574 (1996) Abstract
Apoptosis and the maintenance of homoeostasis in the immune system: B.A. Osborne; Curr. Opin. Immunol. 8, 245 (1996) Abstract
Latent sensitivity to Fas-mediated apoptosis after CD40 ligation may explain activity of CD154 gene therapy in chronic lymphocytic leukemia: P. Chu, et al.; PNAS 99, 3854 (2002) Abstract; Full Text
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-805-010 Revised 29-Apr-08
Monoclonal Antibody to Fas (human) (blocking) (SM1/23)
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SYNONYMS anti-CD95 (human) MAb (blocking) (SM1/23)
anti-APO-1 (human) MAb (blocking) (SM1/23)
anti-TNFRSF 6 (human) MAb (blocking) (SM1/23)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-805-010-C100 Purified 100 µg 259.00 USD Add To Cart
ALX-805-010B-T100 Biotin 100 tests 308.00 USD Add To Cart
ALX-805-010F-T100 FITC 100 tests 308.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: SM1/23
ISOTYPE: Mouse IgG2b
PURITY: ≥95% (SDS-PAGE)
FORMULATION: SM1/23: 1mg/ml in PBS.
SM1/23-Biotin and -FITC: Liquid. In 1ml 50mM TRIS, pH 7.4, containing 100mM NaCl, 1% BSA and 0.02% sodium azide.
IMMUNOGEN: Human Fas (CD95; APO-1).
SPECIFICITY: Recognizes human Fas.
APPLICATION: Flow Cytometry
Functional Application (blocks induction of apoptosis induced by clone SM1/1 (Prod. No. ALX-805-008) or other commercial anti-Fas antibodies).
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-805-008 Revised 13-Jun-06
Monoclonal Antibody to Fas (human) (SM1/1)
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SYNONYMS anti-CD95 (human) MAb (SM1/1)
anti-APO-1 (human) MAb (SM1/1)
anti-TNFRSF 6 (human) MAb (SM1/1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Fas Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-805-008-C100   100 µg 259.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: SM1/1
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
PURITY: >95% (SDS-PAGE)
FORMULATION: Liquid. In PBS.
IMMUNOGEN: Human Fas (CD95; APO-1).
SPECIFICITY: Recognizes human Fas.
APPLICATION: Flow Cytometry
Functional Application:
Induces apoptosis at 100-500 ng/ml in Jurkat cells and SKW6.4 cells if secondary cross-linking with anti-mouse IgG is used. Induces apoptosis in human Fas-transfectants without crosslinking. 
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The antitumor ether lipid ET-18-OCH(3) induces apoptosis through translocation and capping of Fas/CD95 into membrane rafts in human leukemic cells: C. Gajate & F. Mollinedo; Blood 98, 3860 (2001) Abstract; Full Text
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-232 Revised 07-May-08
Monoclonal Antibody to FasL (human) (2C101)
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SYNONYMS anti-CD95L (human) MAb (2C101)
anti-APO-1L (human) MAb (2C101)
anti-CD178 (human) MAb (2C101)
anti-TNFSF 6 (human) MAb (2C101)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-232-C100   100 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 2C101
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE).
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In 0.15M PBS, pH 7.2, containing 1% BSA. Contains no preservatives.
IMMUNOGEN: Recombinant human FasL (CD95L; APO-1L; CD178) (extracellular domain).
SPECIFICITY: Recognizes human FasL.
APPLICATION: Flow Cytometry
Immunocytochemistry
Functional Application (neutralizing - excellent)
Suggested starting dilution is >1:100. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
HANDLING: Do not freeze. Keep sterile.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt et al.; Eur. J. Immunol. 30, 1769 (2000) Abstract
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-231 Revised 07-May-08
Monoclonal Antibody to FasL (human) (5G51)
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SYNONYMS anti-CD95L (human) MAb (5G51)
anti-APO-1L (human) MAb (5G51)
anti-CD178 (human) MAb (5G51)
anti-TNFSF 6 (human) MAb (5G51)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-231-C100   100 µg 340.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 5G51
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In 0.15M PBS, pH 7.2 containing 1% BSA. Contains no preservatives.
IMMUNOGEN: Recombinant human FasL (CD95L; APO-1L; CD178) (extracellular domain).
SPECIFICITY: Recognizes human FasL.
APPLICATION: Flow Cytometry (excellent)
Immunohistochemistry (frozen sections, paraffin sections)
Functional Application (neutralizing).
Suggested starting dilution is 1:100. Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months when stored at +4°C.
HANDLING: Do not freeze/thaw. Keep sterile.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
IL-10 induces apoptosis in human monocytes involving the CD95 receptor/ligand pathway: M. Schmidt et al.; Eur. J. Immunol. 30, 1769 (2000) Abstract
Related Products
Further Categories Containing This Product:
Monoclonal Antibodies • CD Antibodies
 
 
ALX-804-490 Revised 31-Oct-06
Monoclonal Antibody to FasL (human) (GM-5F4)
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SYNONYMS anti-APO-1L (human) MAb (GM-5F4)
anti-CD95L (human) MAb (GM-5F4)
anti-CD178 (human) MAb (GM-5F4)
anti-TNFSF 6 (human) MAb (GM-5F4)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-490-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: GM-5F4
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified by affinity chromatography from cell culture supernatant.
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Antibody in PBS, pH 7.2, containing 0.01% sodium azide.
IMMUNOGEN: Vector containing the cDNA of human FasL (APO-1L; CD95L; CD178)  extracellular domain.
SPECIFICITY: Recognizes human FasL.
APPLICATION: Flow Cytometry.
Optimal conditions must be determined individually.
QUALITY CONTROL: Routinely tested by flow cytometry on BOSC cells transiently transfected with a FasL expression vector.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-124 Revised 26-Feb-07
Monoclonal Antibody to FasL (human) (Mike-1)
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SYNONYMS anti-APO-1L (human) MAb (Mike-1)
anti-CD95L (human) MAb (Mike-1)
anti-CD178 (human) MAb (Mike-1)
anti-TNFSF 6 (human) MAb (Mike-1)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY FasL Antibodies
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-124-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Mike-1
ISOTYPE: Rat IgG2a
SOURCE/HOST: Purified from concentrated hybridoma culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human soluble FasL (CD95L; APO-1L; CD178) (aa 103-281).
SPECIFICITY: Recognizes only membrane bound active (non-cleaved) human FasL and not the inactive soluble FasL.
APPLICATION: Flow Cytometry
Immunocytochemistry
Immunohistochemistry (frozen sections, paraffin sections)
Immunoprecipitation
Note: For Western blot use MAb to FasL (Mike-2) (Prod. No. ALX-804-125).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM