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Chemokines & Cytokines
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ALX-522-008 Revised 18-Sep-07
TNF-α, Soluble (human) (recombinant)
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SYNONYMS Tumor Necrosis Factor-α, Soluble (human) (recombinant)
TNFSF 2, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-008-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~19kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-223) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human and mouse TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-060), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
BIOLOGICAL ACTIVITY: Exerts its biological activity in a concentration range of 0.1-1ng/ml (WEHI 164 cells).
SPECIFIC ACTIVITY: ED50: 0.1ng/ml (WEHI 164 cells)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
A second step of chemotaxis after transendothelial migration: keratinocytes undergoing apoptosis release IFN-gamma-inducible protein 10, monokine induced by IFN-gamma, and IFN-gamma-inducible alpha-chemoattractant for T cell chemotaxis toward epidermis in: S. Klunker, et al.; J. Immunol. 171, 1078 (2003) Abstract; Full Text
Induction of TNF receptor I-mediated apoptosis via two sequential signaling complexes: O. Micheau & J. Tschopp; Cell 114, 181 (2003) Abstract
Insulin-secreting beta-cell dysfunction induced by human lipoproteins: M.E. Roehrich, et al.; J. Biol. Chem. 278, 18368 (2003) Abstract; Full Text
BAFF production by antigen-presenting cells provides T cell co-stimulation: B. Huard, et al.; Int. Immunol. 16, 467 (2004) Abstract
The chemokine receptor CCR7 activates in dendritic cells two signaling modules that independently regulate chemotaxis and migratory speed: L. Riol-Blanco, et al.; J. Immunol. 174, 4070 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P01375: TNF-α (human) (precursor)
AfCS Signalling Gateway link A002291: TNF-α (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-009 Revised 22-Aug-07
TNF-α, Soluble (mouse) (recombinant)
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SYNONYMS Tumor Necrosis Factor-α, Soluble (mouse) (recombinant)
TNFSF 2, Soluble (mouse) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-009-C050   50 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
MW: ~20kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to a linker peptide (8 aa) and a FLAG®-tag.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human, mouse and rat TNF-R1 and less efficiently to TNF-R2. In the presence of cross-linking enhancer (see Set Prod. No. ALX-850-061), TNF-α shows a significantly higher affinity for TNF-R2 than for TNF-R1, mimicking the characteristics of membrane-bound TNF-α.
BIOLOGICAL ACTIVITY: Exerts its biological activity in a concentration range of 0.5-1ng/ml (WEHI 164 cells). 
SPECIFIC ACTIVITY: ED50=0.02ng/ml (WEHI 164 cells)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Conversion of membrane-bound Fas(CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity: P. Schneider, et al.; J. Exp. Med. 187, 1205 (1998) Abstract; Full Text
Inhibition of interleukin 1 receptor/Toll-like receptor signaling through the alternatively spliced, short form of MyD88 is due to its failure to recruit IRAK-4: K. Burns, et al.; J. Exp. Med. 197, 263 (2003) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P06804: TNF-α (mouse) (precursor)
AfCS Signalling Gateway link A002291: TNF-α (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death: C.A. Smith, et al.; Cell 76, 959 (1994) Abstract
Tumor necrosis factor: a pleiotropic cytokine and therapeutic target: K.J. Tracey & A. Cerami; Annu. Rev. Med. 45, 491 (1994) Abstract
The transmembrane form of tumor necrosis factor is the prime activating ligand of the 80 kDa tumor necrosis factor receptor: M. Grell, et al.; Cell 83, 793 (1995) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-046 Revised 27-Mar-06
MegaTNF-α™, Soluble (human) (recombinant)
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SYNONYMS rhsMegaTNF-α™
TNF-α, Soluble (human) (recombinant):ACRP30headless (human)
TNFSF 2, Soluble (human) (recombinant):ACRP30headless (human)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-046-C010   10 µg 440.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~34kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human TNF-α (tumor necrosis factor-α) (aa 85-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human and mouse TNF-R1 and TNF-R2.
BIOLOGICAL ACTIVITY: Killing.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
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General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-047 Revised 27-Mar-06
MegaTNF-α™, Soluble (mouse) (recombinant)
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SYNONYMS rmsMegaTNF-α™
TNF-α, Soluble (mouse) (recombinant):ACRP30headless (mouse)
TNFSF 2, Soluble (mouse) (recombinant):ACRP30headless (mouse)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-047-C010   10 µg 440.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~35kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse TNF-α (tumor necrosis factor-α) (aa 77-235) is fused at the N-terminus to mouse ACRP30headless (aa 11-111) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile distilled water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human and mouse TNF-R1 and TNF-R2.
BIOLOGICAL ACTIVITY: Induces killing at a concentration of <1ng/ml.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-087 Revised 07-Feb-06
Fc (human):TNF-α (human), Soluble (recombinant)
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SYNONYMS Fc (human):Tumor Necrosis Factor-α (human), Soluble (recombinant)
Fc (human):TNFSF2 (human), Soluble (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-087-C010   10 µg 395.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~48kDa
SOURCE/HOST: Produced in CHO cells. The extracellular domain of human TNF-α (aa 85-233) is fused at the N-terminus to the Fc portion of human IgG1 and a linker peptide (20 aa).
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL test; BioWhittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds to human and mouse TNF-R1 and TNF-R2.
BIOLOGICAL ACTIVITY: Induces killing of WEHI-164 cells in a concentration range of 0.5-2ng/ml (does not require an enhancer).
APPLICATION: Flow Cytometry (1:100)
Immunoprecipitation (1:500)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Life and death decisions: secondary complexes and lipid rafts in TNF receptor family signal transduction: J.R. Muppidi, et al.; Immunity 21, 461 (2004) Abstract
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-013 Revised 03-May-07
TNF-R1 (human):Fc (human) (recombinant)
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SYNONYMS TNF-R 60kDa (human):Fc (human) (recombinant)
CD120a (human):Fc (human) (recombinant)
p55 (human):Fc (human) (recombinant)
TNFRSF 1A (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-R1
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-013-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~60kDa (reducing SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human TNF-R1 (aa 1-211) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or carrier protein.
SPECIFICITY: Binds human and mouse TNF-α (tumor necrosis factor-a).
BIOLOGICAL ACTIVITY: Binds to TNF-R1 and triggers various biological effects (including cell death). rhTNF-R1:Fc fusion protein blocks TNF-α-mediated lysis with a inhibitory activity in a concentration range of 10-50ng/ml.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
The lymphotoxin-β receptor is necessary and sufficient for LIGHT-mediated apoptosis of tumor cells: I.A. Rooney, et al.; J. Biol. Chem. 275, 13047 (2000) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P19438: TNF-R1 (human) (precursor)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-014 Revised 03-May-07
TNF-R2 (human):Fc (human) (recombinant)
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SYNONYMS TNF-R 80kDa (human):Fc (human) (recombinant)
CD120b (human):Fc (human) (recombinant)
p75 (human):Fc (human) (recombinant)
TNFRSF 1B (human):Fc (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-R2
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-014-C050   50 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~75kDa (reducing SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human TNF-R2 (aa 1-257) is fused to the Fc portion of human IgG1.
CONCENTRATION: 1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SPECIFICITY: Binds human and mouse TNF-α (tumor necrosis factor-α).
APPLICATION: Detection of membrane-bound TNF-α in combination with PAb to human IgG1 (Prod. No. ALX-211-500 (FITC), ALX-211-501 (R-PE), ALX-211-502 (Biotin)).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Development of improved soluble inhibitors of FasL and CD40L based on oligomerized receptors: N. Holler, et al.; J. Immunol. Methods 237, 159 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P20333: TNF-R2 (human) (precursor)
AfCs Signalling Gateway link A002346: TNF-R2 (mouse)
MANUFACTURER Manufactured by Apotech Corporation.
Related Products
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-522-034 Revised 27-Mar-06
LTα3, Soluble (human) (recombinant)
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SYNONYMS TNF-β, Soluble (human) (recombinant)
TNFSF 1, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY TNF-alpha & TNF Receptors Other Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-034-C010   10 µg 200.00 USD Add To Cart
ALX-522-034-3010 SuperPack 3x10 µg 410.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~25kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of human LTα3 (aa 43-205) is fused at the N-terminus to a linker peptide (10 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human HVEM and human and mouse TNF-R1 and TNF-R2.
BIOLOGICAL ACTIVITY: Kills human TNF-R1 positive cells.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
General Information
Lymphotoxin-α (LTα, TNF-β) is a 25 kDa type II soluble protein belonging to the TNF family. It is expressed as a trimer (LTα3), or with lymphotoxin-β (LTα1β2 or LTα2β1). LTα3 interacts with 3 different members of the TNF-receptor superfamily: HVEM (Prod. No. ALX-522-017), TNF-R1 (Prod. No. ALX-522-013) and TNF-R2 (Prod. No. ALX-522-014). LTα3 is expressed by activated T and B lymphocytes and is a pro-apoptotic cytokine, a pluripotent mediator of inflammtory and immune defense mechanisms, and moreover it plays an important role in regulating the generation of the peripheral lymphoid system.
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P01374: TNF-β (human) (precursor)
AfCS Signalling Gateway link A001438: TNF-β (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins • HVEM & LIGHT / LT-betaR / DcR3 / Related Products
 
 
ALX-522-038 Revised 27-Mar-08
EDA-A1, Soluble (human) (recombinant)
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SYNONYMS Ectodysplasin-A1, Soluble (human) (recombinant)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY EDA & EDAR, Troy / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-038-C010   10 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
MW: ~20kDa (SDS-PAGE).
SOURCE/HOST: Produced in E. coli. The extracellular domain of human EDA-A1 (ectodysplasin-A1) (aa 245-391) is fused at the N-terminus to a linker peptide (9 aa) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: