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ALX-500-001

Revised 09-Oct-08

n-Octyl-β-D-glucopyranoside (analytical grade)
SYNONYMS	OG
PRODUCT LINE	Molecular Biology
PRODUCT CATEGORY	Buffers / Detergents / Solutions

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-500-001-G001		1 g	45.00 USD
ALX-500-001-G005		5 g	160.00 USD
ALX-500-001-G025		25 g	560.00 USD

Product Specification

FORMULA:	C₁₄H₂₈O₆
MW:	292.4
CAS NUMBER:	29836-26-8
PURITY:	≥99%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in water (100mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Keep under inert gas. Hygroscopic.
HAZARD:	IRRITANT.

Product Description

Highly purified nonionic detergent that solubilizes membrane-bound proteins in their native state.

Product Specific Literature References

Widespread distribution of some minor chlorophyll-protein complexes in some green plants and algae: E.L. Camm & B.R. Green; Plant Physiol. 67, 1061 (1981)
Effects of octyl beta-glucoside on insulin binding to solubilized membrane receptors: R.J. Gould, et al.; Biochemistry 20, 6776 (1981) Abstract
Solubilization and reconstitution of the Pseudomonas aeruginosa high affinity branched-chain amino acid transport system: T. Hoshino, et al.; J. Biol. Chem. 267, 21313 (1992) Abstract; Full Text
Reconstitution of the sarcoplasmic reticulum Ca(2+)-ATPase: mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents: D. Levy, et al.; Biochim. Biophys. Acta 1107, 283 (1992) Abstract
Purification and crystallization of the light harvesting LH1 complex from Rhodobacter sphaeroides [retracted by Nunn RS, Artymiuk PJ, Baker PJ, Rice DW, Hunter CN. In: J Mol Biol 1995 Sep 8;252(1):153]: R.S. Nunn, et al.; J. Mol. Biol. 228, 1259 (1992) Abstract

ALX-500-002

Revised 03-Jun-08

n-Octyl-oligo-oxyethylene (biochemical grade)
SYNONYMS	Octyl-POE OPOE
PRODUCT LINE	Molecular Biology
PRODUCT CATEGORY	Buffers / Detergents / Solutions

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-500-002-L005		5 ml	55.00 USD
ALX-500-002-L025		25 ml	220.00 USD
ALX-500-002-L100		100 ml	650.00 USD

Product Specification

FORMULA:	CH₃-(CH₂)₇-O-(CH₂-CH₂-O)_x-H x = 1 to 10
CAS NUMBER:	27252-75-1
APPEARANCE:	Colorless liquid.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HAZARD:	IRRITANT.

Product Description

Detergent for solubilization, purification and crystallization of membrane proteins.

Product Specific Literature References

Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecyl sulfate binding: J.P. Rosenbusch; J. Biol. Chem. 249, 8019 (1974) Abstract; Full Text
Bacterial phosphotransferase system. Solubilization and purification of the glucose-specific enzyme II from membranes of Salmonella typhimurium: B. Erni, et al.; J. Biol. Chem. 257, 13726 (1982) Abstract; Full Text
Solubilized cytochrome c oxidase from Paracoccus denitrificans is a monomer: B. Ludwig, et al.; J. Biol. Chem. 257, 5576 (1982) Abstract; Full Text
Micelle clusters of octylhydroxyoligo(oxyethylenes): M. Zulauf & J.P. Rosenbusch; J. Phys. Chem. 87, 856 (1983)
Stimulatory GTP regulatory unit Ns and the catalytic unit of adenylate cyclase are tightly associated: mechanistic consequences: H. Arad, et al.; PNAS 81, 6579 (1984) Abstract
A new procedure for the isolation of the brain myelin basic protein in a lipid-bound form: P. Riccio, et al.; FEBS Lett. 177, 236 (1984) Abstract
Topology of phage lambda receptor protein. Mapping targets of proteolytic cleavage in relation to binding sites for phage or monoclonal antibodies: S. Schenkman, et al.; J. Biol. Chem. 259, 7570 (1984) Abstract; Full Text
Porin channel triplets merge into single outlets in Escherichia coli outer membranes: A. Engel, et al.; Nature 317, 643 (1985) Abstract
Isolation and crystallization of bacterial porin: R.M. Garavito & J.P. Rosenbusch; Meth. Enzymol. 125, 309 (1986) Abstract
Topographic labelling of pore-forming proteins from the outer membrane of Escherichia coli: M.G.P. Page & J.P. Rosenbusch; Biochem. J. 235, 651 (1986) Abstract
Densely packed beta-structure at the protein-lipid interface of porin is revealed by high-resolution cryo-electron microscopy: H.J. Sass, et al.; J. Mol. Biol. 209, 171 (1989) Abstract
Critical micellar concentrations of detergents: M. Zulauf, et al.; Meth. Enzymol. 172, 528 (1989) Abstract
Membrane protein crystallization: observations and use of short chain phospholipids as amphiphiles: J.L. Eiselé, et al.; J. Cryst. Growth 110, 96 (1990)
The critical role of detergents in the crystallization of membrane proteins: J.P. Rosenbusch; J. Struct. Biol. 104, 134 (1990) Abstract
Crystallization and preliminary X-ray characterization of maltoporin from Escherichia coli: K.A. Stauffer, et al.; J. Mol. Biol. 211, 297 (1990) Abstract
Crystal structures explain functional properties of two E. coli porins: W.S. Cowman, et al.; Nature 358, 727 (1992) Abstract
Lipopolysaccharide regions involved in the activation of Escherichia coli outer membrane protease OmpT: R.A. Kramer, et al.; Eur. J. Biochem. 269, 1746 (2002) Abstract

ALX-500-003

Revised 16-Feb-07

Hecameg®
SYNONYMS	6-O-(N-Heptylcarbamoyl)-methyl-α-D-glucopyranoside
PRODUCT LINE	Molecular Biology
PRODUCT CATEGORY	Buffers / Detergents / Solutions

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-500-003-G001		1 g	45.00 USD
ALX-500-003-G005		5 g	180.00 USD

Product Specification

FORMULA:	C₁₅H₂₉NO₇
MW:	335.4
CAS NUMBER:	115457-83-5
PURITY:	≥99%
APPEARANCE:	White to off-white crytalline solid.
SOLUBILITY:	Soluble in water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C

Product Description

Non-ionic, glucose based detergent for membrane protein purification.

Product Specific Literature References

Synthesis and Characterization of 6-O-(N-Heptylcarbamoyl)-methyl-alpha-D-glucopyranoside, a New Surfactant for Membrane Studies: D. Plusquellec, et al.; Anal. Biochem. 179, 145 (1989) Abstract
Ultrasonic Absorption Studies of Surfactant Exchange between Micelles and Bulk Phase in Aqueous Micellar Solutions of Nonionic Surfactants with a Short Alkyl Chain. 3.Surfactants with a Sugar Head Gp: M. Frindi, et al.; J. Physiol. Chem. 96, 8137 (1992)
Purification and Characterization of Protein H, the Major Porin of Pasteurella multocida: G. Chevalier, et al.; J. Bacteriol. 175, 266 (1993) Abstract
An assessment of the biochemical applications of the non-ionic surfactant HECAMEG(R): M. Begona Ruiz, et al.; Biochim. Biophys. Acta 1193, 301 (1994) Abstract
Overexpression and Purification of the Soluble Polyhydroxyalkanoate Synthase from Alcaligenes eutrophus.: Evidence for a Required Posttranslational Modification for Catalytic Activity: T.U. Gerngross, et al.; Biochemistry 33, 9311 (1994) Abstract
In Chromaffin Cells, the Mammalian Sec1p Homologue Is a Syntaxin 1A-binding Protein Associated with Chromaffin Granules: A. Hodel, et al.; J. Biol. Chem. 269, 8623 (1994) Abstract; Full Text
Purification and Characterization of Adipocyte Heparan Sulfate Proteoglycans with Affinity for Lipoprotein Lipase: K.B. Misra, et al.; J. Biol. Chem. 269, 23838 (1994) Abstract; Full Text
Preliminary Cryocristallographic Study of the Mitochondrial Cytochrome bc1 complex: Improved Crystallization and Flash-cooling of a Large Membrane Protein: J.W. Lee, et al.; J. Mol. Biol. 252, 15 (1995) Abstract
Purification and Characterization of the Cytochrom b6f Complex from Chlamydomonas reinhardtii: Y. Pierre, et al.; J. Biol. Chem. 270, 29342 (1995) Abstract; Full Text
Dimer to monomer conversion of the cytochrome b6 f complex. Causes and consequences: C. Breyton, et al.; J. Biol. Chem. 272, 21892 (1997) Abstract; Full Text
On the presence and role of a molecule of chlorophyll a in the cytochrome b6 f complex: Y. Pierre, et al.; J. Biol. Chem. 272, 21901 (1997) Abstract; Full Text

ALX-500-007

Revised 10-Sep-08

n-Dodecyl-β-D-maltoside (high purity)
SYNONYMS	n-Dodecyl-β-D-maltopyranoside (high purity)
PRODUCT LINE	Molecular Biology
PRODUCT CATEGORY	Buffers / Detergents / Solutions

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-500-007-G001		1 g	90.00 USD
ALX-500-007-G005		5 g	320.00 USD
ALX-500-007-G025		25 g	1'250.00 USD

Product Specification

FORMULA:	C₂₄H₄₆O₁₁
MW:	510.6
CAS NUMBER:	69227-93-6
PURITY:	≥99% (free of α-isomer)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	100mg/ml soluble in water and 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light and moisture. Hygroscopic.

Product Description

Non-ionic detergent similar to n-octyl-β-D-glucopyranoside (Prod. No. ALX-500-001). However, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution. Applicable to membrane-protein solubilization studies.

Product Specific Literature References

Alkyl glucosides as effective solubilizing agents for bovine rhodopsin. A comparison with several commonly used detergents: G.W. Stubbs, et al.; Biochim. Biophys. Acta 426, 46 (1976) Abstract
Alkyl glycoside detergents: synthesis and applications to the study of membrane proteins: T. VanAken, et al.; Meth. Enzymol. 125, 27 (1986) Abstract
Dodecyl maltoside-sodium dodecyl sulfate two-dimensional polyacrylamide gel electrophoresis of chloroplast thylakoid membrane proteins: W.T. Bass & T.M. Bricker; Anal. Biochem. 171, 330 (1988) Abstract
Dodecyl maltoside detergent improves resolution of hepatic membrane proteins in two-dimensional gels: F. Witzmann, et al.; Electrophoresis 12, 687 (1991) Abstract

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ALX-201-130	SuperKillerTRAIL™, Soluble (mouse) (recombinant)

Further Categories Containing This Product:

TRAIL [Apo-2L]

ALX-510-001

Revised 07-Feb-05

TiterMax®
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Antibody Production & Purification

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-510-001-R500		500 µl	150.00 USD
ALX-510-001-L001		1 ml	250.00 USD
ALX-510-001-L005		5 ml	1'300.00 USD
ALX-510-001-L010		10 ml	2'500.00 USD

Product Specification

SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

Product Description

TiterMax^® was designed to meet the specific needs of investigators for an immunoadjuvant that was at least as effective as Freund's Complete Adjuvant, but safer and easier to use [1]. Does not contain mineral oil, proteins, polysaccharides or other microbial products, and thereby minimizes or eliminates the undesirable effects caused by Freund's Complete Adjuvant. Also available: TiterMax^® Gold (Prod. No. ALX-510-002).

Product Specific Literature References

[1] A comparison of commercially available adjuvants for use in research: B. Bennett, et al.; J. Immunol. Meth. 153, 31 (1992) Abstract

Further Categories Containing This Product:

Buffers / Detergents / Solutions

ALX-510-002

Revised 07-Feb-05

TiterMax® Gold
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Antibody Production & Purification

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-510-002-L001		1 ml	145.00 USD
ALX-510-002-L003		3 ml	395.00 USD
ALX-510-002-L005		5 ml	770.00 USD
ALX-510-002-L010		10 ml	1'350.00 USD

Product Specification

SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

Product Description

TiterMax^® (Prod.No. 510-001) was designed to meet the specific needs of investigators for an immunoadjuvant that was at least as effective as Freund's Complete Adjuvant, but safer and easier to use [1]. The key to success of TiterMax^® Gold lies in both the immunostimulatory activity of its new block copolymer, CRL-8300, and its ability to form a stable water-oil emulsion. TiterMax^® Gold can be used with a wide variety of antigens which can be entrapped in a water-in-oil emulsion. Like TiterMax^®, TiterMax^® Gold does not contain mineral oil, proteins, polysaccharides or other microbial products, and thereby minimizes or eliminates the undesirable effects caused by Freund's Complete Adjuvant.

Product Specific Literature References

[1] A comparison of commercially available adjuvants for use in research: B. Bennett, et al.; J. Immunol. Meth. 153, 31 (1992) Abstract

Further Categories Containing This Product:

Buffers / Detergents / Solutions

ALX-700-001

Revised 13-May-08

DOTAP . mesylate
SYNONYMS	N-(1-(2,3-Dioleoyloxy)propyl)-N.N.N-trimethylammonium . mesylate N-(1,2-Dioleyl-dihydroxypropyl)-N.N.N-trimethylammonium . mesylate 1,2-Dioleoyl-3-trimethylammonium-propane mesylate
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Lipid Signalling Pathways Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-700-001-M005		5 mg	20.00 USD
ALX-700-001-M025		25 mg	80.00 USD

Product Specification

FORMULA:	C₄₂H₈₀NO₄. SO₃CH₃
MW:	663.1 . 95.1
CAS NUMBER:	252769-92-9; 144189-73-1
SOURCE/HOST:	Synthetic.
PURITY:	≥98%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in methanol, 100% ethanol, methylene chloride, tetrahydrofuran or isopropanol; insoluble in DMSO or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Packaged under inert gas.

Product Description

Cationic lipid used for transfection of DNA, RNA and other negatively charged molecules into eukaryotic cells.

Product Specific Literature References

Interactions of mammalian cells with lipid dispersions containing novel metabolizable cationic amphiphiles: R. Leventis & J.R. Silvius; Biochim. Biophys. Acta 1023, 124 (1990) Abstract

Further Categories Containing This Product:

Buffers / Detergents / Solutions

ALX-505-007

Revised 09-Sep-08

PBS (endotoxin-free)

FORMULA:	C₁₄H₂₈O₅S
MW:	308.4
CAS NUMBER:	85618-21-9
PURITY:	≥98% (HPLC)
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in water or methanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Hygroscopic. Protect from light.

PURITY DETAIL:	Sterile filtered.
FORMULATION:	Liquid. 20mM HEPES, pH 7.4, containing 300mM NaCl, 0.01% Tween 20, 1% sucrose and 1mM DTT.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C