ALEXIS Biochemicals: Fn14 (human):Fc (human) (recombinant)

Advanced Search

You are here: Product Lines > Proteins > Recombinant Proteins / Fusion Proteins

ALX-522-020

Revised 16-May-06

SuperFasLigand™, Soluble (human) (recombinant)
SYNONYMS	APO-1L, Soluble (human) (recombinant) CD95L, Soluble (human) (recombinant) CD178, Soluble (human) (recombinant) TNFSF 6, Soluble (human) (recombinant)
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	FasL

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-020-C005		5 µg	250.00 USD
ALX-522-020-3005	SuperPack	3x5 µg	500.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat
MW:	~32kDa (nonglycosylated), ~35kDa (glycosylated).
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of human FasL (APO-1L; CD95L; CD178) (aa 103-281) is fused at the N-terminus to a linker peptide (26 aa) and a FLAG^®-tag. Glycosylation of rhsSuperFasLigand^TMis similar to natural human FasL.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with cell culture medium containing 5% fetal calf serum.
SPECIFICITY:	Binds to human, mouse and rat Fas (CD95; APO-1).
BIOLOGICAL ACTIVITY:	Kills Fas-sensitive cells at concentrations of >1ng/ml. Note: Does not require enhancer.
SPECIFIC ACTIVITY:	ED₅₀: 1ng/ml (A20 cells).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

Product Specific Literature References

Fas ligand (CD95L) protects neurons against perforin-mediated T lymphocyte cytotoxicity: I. Medana, et al.; J. Immunol. 167, 674 (2001) Abstract; Full Text
Tumor-cell resistance to death receptor-induced apoptosis through mutational inactivation of the proapoptotic Bcl-2 homolog Bax: H. LeBlanc, et al.; Nat. Med. 8, 274 (2002) Abstract
Inhibition of metalloproteinase cleavage enhances the cytotoxicity of Fas ligand: P.G. Knox, et al.; J. Immunol. 170, 677 (2003) Abstract; Full Text
NF-kappa B is required for surface Ig-induced Fas resistance in B cells: B.R. Schram & T.L. Rothstein; J. Immunol. 170, 3118 (2003) Abstract; Full Text
TNF-related apoptosis-inducing ligand (TRAIL) frequently induces apoptosis in Philadelphia chromosome-positive leukemia cells: K. Uno, et al.; Blood 101, 3658 (2003) Abstract
Fas Ligand Down-Regulates Cytokine-Induced Fas Receptor Expression on Insulinoma (NIT-1), But Not Islet Cells, from Autoimmune Nonobese Diabetic Mice: P. Augstein, et al.; Endocrinology 145, 2747 (2004) Abstract
In vitro engagement of CD3 and CD28 corrects T cell defects in chronic lymphocytic leukemia: M. Bonyhadi, et al.; J. Immunol. 174, 2366 (2005) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Note: Results using rhsSuperFasLigand™ may differ from those obtained with agonistic antibodies!

Swiss-Prot link P48023: FasL (human)
AfCs Signalling Gateway link A002830: FasL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Mature T cells of autoimmune lpr/lpr mice have a defect in antigen-stimulated suicide: J.H. Russel, et al.; PNAS 90, 4409 (1993) Abstract
A role for CD95 ligand in preventing graft rejection: D. Bellgrau, et al.; Nature 377, 630 (1995) Abstract
Melanoma cell expression of Fas(Apo-1/CD95) ligand: implications for tumor immune escape: M. Hahne, et al.; Science 274, 1363 (1996) Abstract
Apoptosis by death factor: S. Nagata; Cell 88, 355 (1997) Abstract
Death receptors: signaling and modulation: A. Ashkenazi & V.M. Dixit; Science 281, 1305 (1998) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • CD Proteins

ALX-522-141

Revised 20-Nov-08

Fc (human):Dectin-1 (mouse) (recombinant)
SYNONYMS	Fc (human):Dendritic Cell-associated C-type Lectin 1 (mouse) (recombinant) Fc (human):β-Glucan Receptor (mouse) (recombinant) Fc (human):C-type Lectin Superfamily Member 12 (mouse) (recombinant) Fc (human):C-type Lectin Domain Family 7 Member A (mouse) (recombinant)
PRODUCT LINE	Other Products
PRODUCT CATEGORY	Lectins Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-141-C050		50 µg	390.00 USD

Product Specification

MW:	~58kDa
SOURCE/HOST:	Produced in CHO cells. The extracellular domain of mouse dectin-1 (aa 71-244) is fused at the N-terminus to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Lonza)
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum or a carrier protein.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

General Information

Dectin-1 is a type II transmembrane protein and a member of the group V C-type lectin-like receptors [1]. It is present on myeloid cells, including macrophages, DCs and neutrophils, and also on certain lymphocytes. Dectin-1 recognizes β-1,3-glucans in a calcium-independent fashion. Through its specificity for β-glucans, dectin-1 can recognize a number of fungal species and may play an important role in antifungal immunity [2].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q6QLQ4: Dectin-1 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

General Literature References

[1] Dectin-1: a signalling non-TLR pattern-recognition receptor: G.D. Brown; Nat. Rev. Immunol. 6, 33 (2006) Abstract
[2] Dectin-1 and its role in antifungal immunity: M. Kimberg & G.D. Brown; Med. Mycol. 46, 631 (2008) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-522-126

Revised 27-Mar-08

Fc (human):Dectin-2 (mouse) (recombinant)
SYNONYMS	Fc (human):Dendritic Cell-associated Lectin 2 (mouse) (recombinant)
PRODUCT LINE	Other Products
PRODUCT CATEGORY	Lectins Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-126-C050		50 µg	390.00 USD

Product Specification

MW:	~55kDa (SDS-PAGE)
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of dectin-2 (aa 44-209) is fused at the N-terminus to the Fc portion of human IgG1.
CONCENTRATION:	1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 50µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 6 months after receipt when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.

General Information

Dectin-2 (dendritic cell-associated C-type lectin-2) is a lectin that has been reported to bind to zymosan as well as to hyphae of organisms such as Candida albicans. Dectin-2 exhibits specific recognition of high-mannose structures. Activation of Dectin-2 leads to phosphorylation of FcRg and up-regulated secretion of TNFα and IL-1ra. Dectin-2 might act on inflammatory macrophages and DC to detect pathogenic organisms and possibly endogenous glycoproteins.
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9JKF4: Dectin-2 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-522-131

Revised 27-Mar-08

Fc (human):RANKL, Soluble (mouse) (rec.)
SYNONYMS	Fc (human):ODF, Soluble (mouse) (recombinant) Fc (human):OPGL, Soluble (mouse) (recombinant) Fc (human):TRANCE, Soluble (mouse) (recombinant) Fc (human):TNFSF 11, Soluble (mouse) (recombinant) Fc (human):CD254, Soluble (mouse) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	RANK & RANKL

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-522-131-C010		10 µg	390.00 USD

Product Specification

MW:	~55kDa
SOURCE/HOST:	Produced in HEK 293 cells. The extracellular domain of mouse RANKL (aa 157-316) is fused to the Fc portion of human IgG1.
CONCENTRATION:	0.1mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized. Contains PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION:	Reconstitute with 100µl sterile water.
SPECIFICITY:	Binds to mouse RANK.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information


BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link O35235: TNF11 (mouse)
MANUFACTURER	Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-249

Revised 18-Mar-08

FEN-1 (human) (recombinant)
SYNONYMS	Flap Structure-specific Endonuclease 1 (human) (recombinant) MF1 (human) (recombinant) Maturation Factor 1 (human) (recombinant) DNase IV (human) (recombinant)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA Endonucleases / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-249-C010		10 µg	260.00 USD

Product Specification

MW:	~42kDa
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 1mM DTT, 1mM EDTA and 0.02% Nonidet P40.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least 4 weeks when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Product Specific Literature References

Flap endonuclease 1: a novel tumour suppresser protein: G. Henneke, et al.; TIBS 28, 384 (2003) Abstract
The Fen1 extrahelical 3’-flap pocket is conserved from archaea to human and regulates DNA substrate specificity: E. Friedrich-Heineken and U. Hubscher; Nucl. Acids Res. 32, 2520 (2004) Abstract; Full Text

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link P39748: FEN-1 (human)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-407

Revised 27-Mar-08

FGF-21 (human) (recombinant) (His)
SYNONYMS	Fibroblast Growth Factor 21 (human) (recombinant) (His)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Fibroblast Growth Factors [FGFs] & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-407-C010		10 µg	170.00 USD
ALX-201-407-C050		50 µg	440.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. Mature human fibroblast growth factor 21 (FGF-21) (aa 1-209) is fused at the C-terminus to a His-tag.
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Product Images

Please click on thumbnails to enlarge.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9NSA1: Fibroblast Growth Factor 21 (human)
MANUFACTURER	Manufactured by AdipoGen, Inc.

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-201-418

Revised 09-Oct-08

FGF-23 (human):Fc (human) (recombinant)
SYNONYMS	Fibroblast Growth Factor 23 (human):Fc (human) (recombinant) Tumor-derived Hypophosphatemia-inducing Factor (human):Fc (human) (recombinant)
PRODUCT LINE	Chemokines & Cytokines
PRODUCT CATEGORY	Fibroblast Growth Factors [FGFs] & Receptors / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-418-C010		10 µg	230.00 USD
ALX-201-418-C050		50 µg	560.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. The original signal peptide and the mature peptide of human FGF-23 (aa 1-251) are fused at the C-terminus to the Fc portion of human IgG.
CONCENTRATION:	1mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2μm-filtered solution in PBS.
ENDOTOXIN CONTENT:	<1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

Please click on thumbnails to enlarge.

General Information

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9GZV9: Fibroblast Growth Factor-23 (human)
MANUFACTURER	Manufactured by AdipoGen, Inc.

ALX-201-437	FGF-23 (mouse):Fc (human) (recombinant)
ALX-201-435	FGF-23 (R179Q Mutant) (human) (recombinant) (His)
ALX-201-423	FGF-23 (human) (recombinant) (His)

ALX-201-418	FGF-23 (human):Fc (human) (recombinant)
ALX-201-435	FGF-23 (R179Q Mutant) (human) (recombinant) (His)
ALX-201-423	FGF-23 (human) (recombinant) (His)

SOURCE/HOST:	Produced in HEK 293 cells. Mature mouse fibroblast growth factor 21 (FGF-21) (aa 1-210) is fused at the C-terminus to a His-tag.
CONCENTRATION:	0.5mg/ml
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9JJN1: Fibroblast Growth Factor 21 (mouse)
MANUFACTURER	Manufactured by AdipoGen, Inc.

BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9EPC2: Fibroblast Growth Factor-23 (mouse)
MANUFACTURER	Manufactured by AdipoGen, Inc.