• Home
  • Sitemap
  • Help
  • Technical Support
  • Contact
APRIL
You are here: Product Lines > Chemokines & Cytokines > APRIL
Toolbar - View Selection
 
Items 9 of 9
ALX-522-035 Revised 14-Aug-08
MegaAPRIL™, Soluble (human) (recombinant)
Add to Clipboard
SYNONYMS APRIL, Soluble (human) (recombinant):ACRP30headless (human)
A-Proliferation-inducing Ligand (human) (recombinant):ACRP30headless (human)
TNFSF 13, Soluble (human) (recombinant):ACRP30headless (human)
CD256, Soluble (human) (recombinant):ACRP30headless (human)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-035-3010   3x10 µg 460.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
MW: ~34kDa (SDS-PAGE).
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of human APRIL (aa 88-233) is fused at the N-terminus to human ACRP30headless (aa 16-108) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: <0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human and mouse BCMA and TACI. Binds to proteoglycans.
BIOLOGICAL ACTIVITY: Stimulates proliferation of serum-starved NIH 3T3 cells. Stimulates B cell proliferation.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL: M.B. Litinskiy, et al.; Nat. Immunol. 3, 822 (2002) Abstract
APRIL modulates B and T cell immunity: J.V. Stein, et al.; J. Clin. Invest. 109, 1587 (2002) Abstract; Full Text
Lymphoma B cells evade apoptosis through the TNF family members BAFF/BLyS and APRIL: B. He, et al.; J. Immunol. 172, 3268 (2004) Abstract; Full Text
Potential role of APRIL as autocrine growth factor for megakaryocytopoiesis: D. Bonci, et al.; Blood 104, 3169 (2004) Abstract; Full Text
BAFF regulates B cell survival by downregulating the BH3-only family member Bim via the ERK pathway: A. Craxton, et al.; J. Exp. Med. 202, 1363 (2005) Abstract
APRIL and BAFF promote increased viability of replicating human B2 cells via mechanism involving cyclooxygenase 2: P.K. Mongini, et al.; J. Immunol. 176, 6736 (2006) Abstract; Full Text
TACI regulates IgA production by APRIL in collaboration with HSPG: D. Sakurai, et al.; Blood 109 , 2961 (2007) Abstract
BAFF and APRIL support chronic lymphocytic leukemia B-cell survival through activation of the canonical NF-kappaB pathway: T. Endo, et al.; Blood 109, 703 (2007) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link O75888: APRIL (human)
AfCS Signalling Gateway link A000305: APRIL (mouse)

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth: M. Hahne, et al.; J. Exp. Med. 188, 1185 (1998) Abstract
Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI: S.A. Marsters, et al.; Curr. Biol. 10, 785 (2000) Abstract
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000) Abstract
Tumor necrosis factor (TNF) receptor superfamily member TACI is a high affinity receptor for TNF family members APRIL and BLyS: Y. Wu, et al.; J. Biol. Chem. 275, 35478 (2000) Abstract
APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity: G. Yu, et al.; Nat. Immunol. 1, 252 (2000) Abstract
B-cell maturation protein, which binds the tumor necrosis factor family members BAFF and APRIL, is dispensable for humoral immune responses: S. Xu & K.P. Lam; Mol. Cell. Biol. 21, 4067 (2001) Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-522-092 Revised 27-Mar-06
MegaAPRIL™, Soluble (mouse) (H98) (recombinant)
Add to Clipboard
SYNONYMS rmsMegaAPRIL™ (H98)
APRIL, Soluble (mouse) (recombinant):ACRP30headless (mouse) (H98)
A-Proliferation-inducing Ligand (mouse) (recombinant):ACRP30headless (mouse) (H98)
TNFSF 13, Soluble (mouse) (recombinant):ACRP30headless (mouse) (H98)
CD256, Soluble (mouse) (recombinant):ACRP30headless (mouse) (H98)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-522-092-3010   3x10 µg 440.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
SOURCE/HOST: Produced in HEK 293 cells. The extracellular domain of mouse APRIL (aa 98-232) is fused at the N-terminus to a mouse ACPR30headless (aa 18-110) and a FLAG®-tag.
CONCENTRATION: 0.1mg/ml after reconstitution.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized. Contains PBS.
ENDOTOXIN CONTENT: ≤0.1EU/µg purified protein (LAL test; Bio Whittaker).
RECONSTITUTION: Reconstitute with 100µl sterile water. Further dilutions should be made with medium containing 5% fetal calf serum.
SPECIFICITY: Binds to human and mouse BCMA and TACI. Does not bind to proteoglycans.
BIOLOGICAL ACTIVITY: Stimulates B cell proliferation.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at -20°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth: M. Hahne, et al.; J. Exp. Med. 188, 1185 (1998) Abstract
Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI: S.A. Marsters, et al.; Curr. Biol. 10, 785 (2000) Abstract
A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth: P. Rennert, et al.; J. Exp. Med. 192, 1677 (2000) Abstract
Tumor necrosis factor (TNF) receptor superfamily member TACI is a high affinity receptor for TNF family members APRIL and BLyS: Y. Wu, et al.; J. Biol. Chem. 275, 35478 (2000) Abstract; Full Text
APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity: G. Yu, et al.; Nat. Immunol. 1, 252 (2000) Abstract
Identification of proteoglycans as the APRIL-specific binding partners: K. Ingold, et al.; J. Exp. Med. 201, 1375 (2005) Abstract
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-804-148 Revised 31-Oct-06
Monoclonal Antibody to APRIL (human) (Aprily-1)
Add to Clipboard
SYNONYMS anti-A-Proliferation-inducing Ligand (human) MAb (Aprily-1)
anti-TNFSF 13 (human) MAb (Aprily-1)
anti-CD256 (human) MAb (Aprily-1)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-148-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Aprily-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: 95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human APRIL (aa 93-233).
SPECIFICITY: Recognizes human APRIL. Does not cross-react with mouse APRIL.
APPLICATION: Flow Cytometry
Immunoprecipitation
Western Blot (excellent)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-607.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
BAFF production by antigen-presenting cells provides T cell co-stimulation: Int. Immunol.; B. Huard, et al. 16, 467 (2004) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. (2002) Abstract
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-844 Revised 20-Jun-08
Monoclonal Antibody to APRIL (human) (Aprily-2)
Add to Clipboard
SYNONYMS anti-A-Proliferation-inducing Ligand (human) MAb (Aprily-2)
anti-TNFSF 13 (human) MAb (Aprily-2)
anti-CD256 (human) MAb (Aprily-2)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-844-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Aprily-2
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: 95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human APRIL (aa 93-233).
SPECIFICITY: Recognizes human APRIL.
APPLICATION: Immunohistochemistry (paraffin sections (1:500))
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
The source of APRIL up-regulation in human solid tumor lesions: P. Mhawech-Fauceglia, et al.; J. Leukoc. Biol. 80, 697 (2006) Abstract
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. (2002) Abstract
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-801 Revised 07-Aug-08
Monoclonal Antibody to APRIL (human) (Aprily-5)
Add to Clipboard
SYNONYMS anti-A-Proliferation-inducing Ligand (human) MAb (Aprily-5)
anti-TNFSF 13 (human) MAb (Aprily-5)
anti-CD256 (human) MAb (Aprily-5)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-801-C100   100 µg 320.00 USD Add To Cart
ALX-804-801B-C100 Biotin 100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Aprily-5
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: 95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human APRIL (aa 93-233).
SPECIFICITY: Recognizes human APRIL. Does not cross-react with mouse APRIL.
APPLICATION: ELISA (detection)
Immunoprecipitation
Western Blot (excellent)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep conjugated formats at +4°C. Do not freeze. Protect from light.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-607.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-149 Revised 31-Mar-08
Monoclonal Antibody to APRIL (human) (Aprily-8)
Add to Clipboard
SYNONYMS anti-A-Proliferation-inducing Ligand (human) MAb (Aprily-8)
anti-TNFSF 13 (human) MAb (Aprily-8)
anti-CD256 (human) MAb (Aprily-8)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-149-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Aprily-8
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE).
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human APRIL (aa 93-233).
SPECIFICITY: Recognizes human APRIL and TWE-PRIL. Does not cross-react with mouse APRIL and TWE-PRIL.
APPLICATION: Flow Cytometry
Immunocytochemistry (excellent)
Immunohistochemistry (paraffin sections)
Immunoprecipitation
Western Blot (excellent)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least one year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-607.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Expression of TNF-superfamily members BAFF and APRIL in breast cancer: Immunohistochemical study in 52 invasive ductal breast carcinomas: V. Pelekanou, et al.; BMC Cancer 8, 76 (2008) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
BAFF and APRIL: A Tutorial on B Cell Survival: F. Mackay, et al.; Annu. Rev. Immunol. (2002) Abstract
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-141 Revised 18-Sep-06
Monoclonal Antibody to APRIL (Sacha-1)
Add to Clipboard
SYNONYMS anti-A-Proliferation-inducing Ligand MAb (Sacha-1)
anti-TNFSF 13 MAb (Sacha-1)
anti-CD256 MAb (Sacha-1)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-141-C100   100 µg 290.00 USD Add To Cart
Inquire
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Sacha-1
ISOTYPE: Rat IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human APRIL (aa 105-250) fused to human ACRP30headless (aa 16-108) (Prod. No. ALX-522-035).
SPECIFICITY: Recognizes human and mouse APRIL.
APPLICATION: Flow Cytometry (excellent for mouse APRIL, also stains human APRIL)
Immunocytochemistry
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at +4°C undiluted.
HANDLING: Do not freeze/thaw.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-804-804 Revised 13-Mar-07
Monoclonal Antibody to APRIL (Sacha-2)
Add to Clipboard
SYNONYMS anti-A-Proliferation-inducing Ligand MAb (Sacha-2)
anti-TNFSF 13 MAb (Sacha-2)
anti-CD256 MAb (Sacha-2)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-804-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Sacha-2
ISOTYPE: Rat IgG2a
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human APRIL (aa 105-250) fused to human ACRP30headless (aa 16-108) (Prod. No. ALX-522-035).
SPECIFICITY: Recognizes human and mouse APRIL.
APPLICATION: ELISA (capture)
Flow Cytometry
Immunocytochemistry
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for at least 6 months after receipt when stored at +4°C undiluted.
HANDLING: Do not freeze/thaw.
Product Images
Please click on thumbnails to enlarge.
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
CD Antibodies • Monoclonal Antibodies
 
 
ALX-210-950 Revised 29-Oct-07
Polyclonal Antibody to APRIL (Membrane Form) (human) (AT125)
Add to Clipboard
SYNONYMS anti-A-Proliferation-inducing Ligand (Membrane Form) (human) PAb (AT125)
anti-TNFSF 13 (Membrane Form) (human) PAb (AT125)
anti-CD256 (Membrane Form) (human) PAb (AT125)
PRODUCT LINE Chemokines & Cytokines
PRODUCT CATEGORY APRIL
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-210-950-C100   100 µg 350.00 USD Add To Cart