ALEXIS Biochemicals: Coelenterazine

ALX-620-063

Revised 03-Feb-05

Coelenterazine
SYNONYMS	2-[(4-Hydroxyphenyl)methyl]-6-(4-hydroxyphenyl)-8-(phenylmethyl)-imidazo [1,2-a] pyrazin-3-(7H)-one
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Peroxynitrite / Scavengers / Detection

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-620-063-C050		50 µg	110.00 USD

Product Specification

FORMULA:	C₂₆H₂₁N₃O₃
MW:	423.5
CAS NUMBER:	55779-48-1
PURITY:	≥95%
APPEARANCE:	Brown solid.
SOLUBILITY:	Slightly soluble in methanol or ethanol. Avoid DMSO.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. Keep under inert gas. Keep calcium free when stored in solution.

Product Description

Cell permeable, very sensitive and specific chemiluminescence probe of the superoxide anion and peroxynitrite. When oxidized by oxygen it emits blue light at 446nm when Ca²⁺ binds to the complex. Powerful antioxidant. See also 2-(4-Dehydroxy)coelenterazine (Prod. No. ALX-620-062).

Product Specific Literature References

Use of calcium-regulated photoproteins as intracellular Ca2+ indicators: J.R. Blinks; Meth. Enzymol. 172, 164 (1989) Abstract
Slow calcium waves accompany cytokinesis in medaka fish eggs: R.A. Fluck, et al.; J. Cell Biol. 115, 1259 (1991) Abstract
Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils: M. Lucas & F. Solano; Anal. Biochem. 206, 273 (1992) Abstract
Aequorin-expressing mammalian cell lines used to report Ca2+ mobilization: D. Button & M. Brownstein; Cell Calcium 14, 663 (1993) Abstract
Imaging [Ca2+]i with aequorin using a photon imaging detector: A.L. Miller, et al.; Meth. Cell Biol. 40, 305 (1994) Abstract
Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA: S. Saran, et al.; FEBS Lett. 337, 43 (1994) Abstract
The origins of marine bioluminescence: turning oxygen defence mechanisms into deep-sea communication tools: J.F. Rees, et al.; J. Exp. Biol. 201, 1211 (1998) Abstract; Full Text
Chemiluminescent detection of oxidants in vascular tissue. Lucigenin but not coelenterazine enhances superoxide formation: M.M. Tarpey, et al.; Circ. Res. 84, 1203 (1999) Abstract

Further Categories Containing This Product:

Dyes / Stains / Fluorescent Probes / Fluorescent Labels • Antioxidants, Flavonoids & Free Radical Scavengers Other Products • Luciferin & Luciferase / Related Products • Calcium Indicators / Probes • Oxidative Stress Markers & Reagents / Related Products

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