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ALX-260-122 Revised 12-Jun-03
Mca-Pro-Cha-Gly-Nva-His-Ala-Dap(Dnp)-NH2
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Substrates
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ALX-260-122-M001   1 mg 190.00 USD Add To Cart
Product Specification
FORMULA: C51H65N13O15
MW: 1100.2
PURITY: ≥97%
APPEARANCE: Yellow solid.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
Product Description
Fluorogenic substrate for MMP-13.
Product Specific Literature References
Biochemical characterization of human collagenase-3: V. Knäuper, et al.; J. Biol. Chem. 271, 1544 (1996) Abstract; Full Text
 
 
ALX-260-123 Revised 14-Dec-04
Mca-Pro-Leu-Ala-Nva-Dap(Dnp)-Ala-Arg-NH2
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Substrates
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ALX-260-123-M001   1 mg 190.00 USD Add To Cart
Product Specification
MW: 1093.0
PURITY: ≥97%
APPEARANCE: Yellow solid.
SOLUBILITY: Soluble in DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Keep cool and dry. Protect from light.
Product Description
Fluorogenic substrate for MMP-2.
 
 
ALX-260-125 Revised 12-Jun-03
Mca-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2
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PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMP Substrates
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ALX-260-125-M001   1 mg 190.00 USD Add To Cart
ALX-260-125-M005   5 mg 684.00 USD Add To Cart
Product Specification
FORMULA: C49H68N14O15
MW: 1093.2
PURITY: ≥97%
APPEARANCE: Yellow solid.
SOLUBILITY: DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Keep cool and dry. Protect from light.
Product Description
Fluorogenic substrate for MMP-2 and MMP-7.
 
 
ALX-201-381 Revised 18-Mar-08
MFAP4 (human) (recombinant)
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SYNONYMS Microfibril-associated Glycoprotein 4 (human) (recombinant)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Adhesion Molecules & Receptors Other Products
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ALX-201-381-C010   10 µg 180.00 USD Add To Cart
ALX-201-381-C050   50 µg 480.00 USD Add To Cart
Product Specification
MW: ~36kDa
SOURCE/HOST: Produced in HEK 293 cells. Human MFAP4 [microfibril-associated glycoprotein 4] (aa 1-255) is fused at the C-terminus to a FLAG®-tag.
CONCENTRATION: 1mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT: <0.1EU/µg protein (LAL-test).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
ANGPTL2, ANGPTL3, ANGPTL5 and ANGPTL7 have recently been shown to stimulate the ex vivo expansion of the hematopoietic stem cells. The same report showed that the microfibril-associated glycoprotein 4 (MFAP4), which exhibits sequence similarity to ANGPTLa, also posesses such ex vivo expansion activity [1].
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P55083: MFAP4 (human) [Precursor]

FLAG is a registered trademark of Sigma-Aldrich Co.
MANUFACTURER Manufactured by AdipoGen, Inc.
General Literature References
[1] Angiopoietin-like proteins stimulate ex vivo expansion of hematopoietic stem cells: C.C. Zhang, et al.; Nat. Med. 12, 240 (2006) Abstract
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Recombinant Proteins/Fusion Proteins
 
 
ALX-201-173 Revised 18-May-06
Midkine (human) (recombinant) (His)
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SYNONYMS ARAP (human) (recombinant) (His)
Amphiregulin-associated Protein (human) (recombinant) (His)
Neurite Outgrowth-promoting Factor 2 (human) (recombinant) (His)
RIHB (human) (recombinant) (His)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY Heparin Binding Factors/Related Products
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ALX-201-173-C100   100 µg 532.00 USD Add To Cart
Product Specification
MW: 14.6kDa (for the fusion protein)
SOURCE/HOST: Produced in E. coli. Human midkine is fused at the N-terminus to a His-tag.
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Sterile filtered.
FORMULATION: Lyophilized from 0.05M phosphate buffer, pH 7.2, containing 0.1M sodium chloride..
RECONSTITUTION: Reconstitute with 200µl of PBS, pH 7.2.
APPLICATION: ELISA
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Reconstituted protein is stable for 2 weeks when stored at +4°C.
HANDLING: After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Midkine is a retinoic acid inducible heparin binding protein. Promotes neuronal survival and neurite outgrowth and plays a role in carcinogenesis and tissue remodeling. Midkine expression is induced during remodeling after bone fractures.
Further Categories Containing This Product:
Recombinant Proteins/Fusion Proteins
 
 
ALX-270-088 Revised 07-Apr-05
ML-7 . hydrochloride
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SYNONYMS 1-(5-Iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine . HCl
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Tyrosine Kinase Inhibitors
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ALX-270-088-M005   5 mg 40.00 USD Add To Cart
ALX-270-088-M025   25 mg 160.00 USD Add To Cart
Product Specification
FORMULA: C15H17IN2O2S . HCl
MW: 416.0 . 36.5
CAS NUMBER: 110448-33-4
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO (can be further diluted with water), 100% ethanol (warm) or methanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Inhibits smooth-muscle myosin light chain kinase, protein kinase C (PKC), and cAMP-dependent protein kinase (PKA).
Product Specific Literature References
Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase: M. Saitoh, et al.; J. Biol. Chem. 262, 7796 (1987) Abstract; Full Text
Further Categories Containing This Product:
Myosin Light Chain Kinase Inhibitors • PKC Inhibitors • PKA Inhibitors
 
 
ALX-270-084 Revised 28-Jan-05
ML-9 . hydrochloride
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SYNONYMS 1-(5-Chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine . HCl
PRODUCT LINE Neurobiology
PRODUCT CATEGORY Myelin Basic Protein/Related Products
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ALX-270-084-M010   10 mg 65.00 USD Add To Cart
ALX-270-084-M025   25 mg 125.00 USD Add To Cart
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Product Specification
FORMULA: C15H17ClN2O2S . HCl
MW: 324.8 . 36.5
CAS NUMBER: 105637-50-1
PURITY: ≥98%
APPEARANCE: White solid.
SOLUBILITY: Soluble in water or DMSO.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Inhibitor of myosin light chain kinase, protein kinase C (PKC) and protein cAMP-dependent protein kinase (PKA). Also inhibits catecholamine secretion in intact and permeabilized chromaffin cells.
Product Specific Literature References
The modulatory role of myosin light chain phosphorylation in human platelet activation [published erratum appears in Biochem Biophys Res Commun 1987 Jan 15;142(1):287]: M. Saitoh, et al.; BBRC 140, 280 (1986) Abstract
Effects of myosin light-chain kinase inhibitor on catecholamine secretion from rat pheochromocytoma PC12h cells: T. Nagatsu, et al.; BBRC 143, 1045 (1987) Abstract
Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase: M. Saitoh, et al.; J. Biol. Chem. 262, 7796 (1987) Abstract; Full Text
Effects of HA1077, a protein kinase inhibitor, on myosin phosphorylation and tension in smooth muscle: M. Seto, et al.; Eur. J. Pharmacol. 195, 267 (1991) Abstract
Naphthalenesulfonamide derivatives ML9 and W7 inhibit catecholamine secretion in intact and permeabilized chromaffin cells: J.A. Reig, et al.; Neurochem. Res. 18, 317 (1993) Abstract
Further Categories Containing This Product:
Myosin Light Chain Kinase Inhibitors • PKC Inhibitors • PKA Inhibitors
 
 
ALX-200-422 Revised 07-Nov-07
MMP-9 Proenzyme (human), Monomer
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SYNONYMS Gelatinase B Proenzyme (human), Monomer
Matrix Metalloproteinase 9 Proenzyme (human), Monomer
Collagenase (92kDa Type IV) Proenzyme (human), Monomer
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
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ALX-200-422-C005   5 µg 121.00 USD Add To Cart
Product Specification
MW: ~92kDa.
EC: 3.4.24.35
SOURCE/HOST: Isolated from stimulated human neutrophil granulocytes (buffy coat). Requires activation.
CONCENTRATION: 100µg/ml 
PURITY: ≥95% (SDS-PAGE, Western blot)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.0, containing 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% Brij 35, and 0.05% NaN3.
SPECIFIC ACTIVITY: ≥1’300mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol 2,4-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for 1 week when stored at +4°C and for serveral weeks when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
Product Description
MMP-9 hydrolyzes the extracellular matrix, e.g. collagen types IV, V and IX and gelatin.
Product Specific Literature References
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Progelatinase B forms from human neutrophils. complex formation of monomer/lipocalin with TIMP-1: H. Kolkenbrock, et al.; Biol. Chem. 377, 529 (1996) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Precursor enzyme needs activation using 2mM AMPA (aminophenylmercuric acetate) for 60-120 min. or 100µg/ml TPCK-trypsin for 30 min. at 37°C. If required trypsin can be inhibited by incubation with 1µl of 1mg/ml aprotinin solution for 10 min. at 25°C.
If working with MMP-9/TIMP-1 complexes, it is recommended to use stromelysin-1 (MMP-3) for activation. Incubation at 37°C for 2 hours at 40:1 (MMP-9:stromelysin-1) will remove only the propeptide to give the active form of the enzyme.

The active enzyme is inhibited by TIMP-1 (tissue inhibitor of matrix metalloproteinase-1) and by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link P14780: MMP-9 (human) (precursor)
AfCS Signalling Gateway link A001479: MMP-9 (mouse)
Further Categories Containing This Product:
Natural Proteins • Enzymes
 
 
ALX-200-418 Revised 23-Jul-07
MMP-1 Proenzyme (human)
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SYNONYMS Collagenase (Type I) (human)
Matrix Metalloproteinase 1 (human)
Interstitial Collagenase (human)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
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ALX-200-418-C005   5 µg 182.00 USD Add To Cart
Product Specification
MW: ~56kDa.
EC: 3.4.24.7
SOURCE/HOST: Isolated from human rheumatoid synovial fibroblasts. Requires activation.
CONCENTRATION: 100µg/ml (Pierce-BCA).
PURITY: ≥90% (SDS-PAGE, Western blot)
PURITY DETAIL: No other MMP contaminants are detectable.
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.0, 300mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ35 and 0.05% sodium azide.
SPECIFIC ACTIVITY: ≥100mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0. APMA activation 1 hour at 37°C; enzyme dilution for assay 1:10/5-10µl for enzyme activity assay - incubation time 30min.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
Secreted forms of human neutrophil collagenase: K.A. Hasty, et al.; J. Biol. Chem. 261, 5645 (1986) Abstract; Full Text
Human fibroblast collagenase: glycosylation and tissue-specific levels of enzyme synthesis: S.M. Wilhelm, et al.; PNAS 83, 3756 (1986) Abstract
Stepwise activation mechanisms of the precursor of matrix metalloproteinase 3 (stromelysin) by proteinases and (4-aminophenyl)mercuric acetate: H. Nagase, et al.; Biochemistry 29, 5783 (1990) Abstract
Mechanisms of activation of tissue procollagenase by matrix metalloproteinase 3 (stromelysin): K. Suzuki, et al.; Biochemistry 29, 10261 (1990) Abstract
Stepwise activation mechanisms of the precursors of matrix metalloproteinases 1 (tissue collagenase) and 3 (stromelysin): H. Nagase, et al.; Biomed. Biochim. Acta 50, 749 (1991) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
The activity of collagenase-1 is required for keratinocyte migration on a type I collagen matrix: B.K. Pilcher, et al.; J. Cell Biol. 137, 1445 (1997) Abstract
The catalytic domain of activated collagenase I (MMP-1) is absolutely required for interaction with its specific inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1): R. Vallon, et al.; Eur. J. Biochem. 244, 81 (1997) Abstract
General Information
MMP-1, the classical matrix metalloproteinase, is expressed by a large number of cell types. As an example, in basal keratinocytes migrating across the dermal matrix the enzyme is invariably expressed and cleaves fibrilliar collagen type I. It was shown that the interaction of the α2β1 integrin with dermal collagen mediates induction of MMP-1 in keratinocytes at the onset of healing and that the activity of MMP-1 is needed to initiate cell movement. The cleavage of dermal collagen provides keratinocytes with a mechanism to maintain their directionality during reepithelialization (Pilcher et al. 1997).
The MMP-1 zymogens of ~56/52kDa can be activated by a stepwise mechanism through which sequential processing events occur in the propeptide region. Both proenzymes can be activated by limited digestion with trypsin or by treatment with APMA generating their respective active enzyme forms of ~46/42kDa (Wilhelm et al. 1986).
BACKGROUND/TECHNICAL INFORMATION Activity:
We recommend to dilute this enzyme preparation at least 1:10 and to take 5µl or less for the determination of activity. Specific activity can be assayed with the synthetic substrate N-(2,4)-dinitrophenyl-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg (Dnp-peptide) (Masui et al.). Substrate concentration should be 0.5mg/ml in buffer 50mM TRIS-HCl, pH 7.0, 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ35, 0.05% sodium azide, containing 0.05mg/ml albumin. One unit MMP catalyzes the hydrolysis of 1µmol Dnp-peptide/min at 37°C and pH 7.0.
We recommend to employ the fluorogenic substrate (7-Methoxycoumarin-4-yl)acetyl-Pro-Leu-Gly-Leu-N-β-Dnp-L-α,β-diaminopropionyl-Ala-Arg-NH2) (Knight et al. 1992). The hydrolysis of the Gly-Leu bond separates the highly fluorescent (7-Methoxycoumarin-4-yl)acetyl group from the 2,4-dinitrophenyl resulting in an increase of fluorogenic intensity. The substrate should be kept as a 9.15mM stock solution in DMSO (10mg/ml). In the assay the substrate concentration should be ~25mM. The assay can be performed in a 96-well microtiter plate (200µl per well) suitable for fluorogenic measurements (excitation wavelength of 328nm; emission wavelength of 393nm).

Activation:
Do not dilute enzyme for activation! Activation is required by trypsin (2µl trypsin; 1mg/ml) for 10-20 min. at 37°C and stopped by the addition of 10µl trypsin-inhibitor (2mg/ml) or aprotinin or TLCK. Activation can also be done by 2mM (final concentration) APMA for 60 min. at 37°C.

Inhibitors:
Only  the activated and not the latent forms of wild-type MMP-1 protein is able to form a complex with TIMP-1. Quite in contrast to MMP-2 (gelatinase A) and MMP-9 (gelatinase B), in MMP-1 the C-terminal hemopexin domain does not interact with TIMP-1. The integrity of the catalytic domain of MMP-1 and its ability to bind Zn2+ is absolutely required for complex formation with TIMP-1, which further underlines the importance of this region for proper regulation of enzymatic activity of MMP-1 (Vallon et al. 1997). Therefore, the enzyme is also inhibited by chelators of divalent cations like EDTA or o-phenantroline.

Swiss-Prot link P03956: MMP-1 (human)
AfCS Signalling Gateway link A001461: MMP-1 (mouse)
Further Categories Containing This Product:
Natural Proteins • Enzymes
 
 
ALX-200-419 Revised 20-Mar-08
MMP-2 Proenzyme (human)
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SYNONYMS Gelatinase A (human)
Matrix Metalloproteinase 2 (human)
Collagenase (72kDa Type IV) (human)
PRODUCT LINE Cytoskeleton
PRODUCT CATEGORY MMPs
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ALX-200-419-C005   5 µg 166.00 USD Add To Cart
Product Specification
MW: ~72kDa.
EC: 3.4.24.24
SOURCE/HOST: Isolated from human rheumatoid synovial fibroblasts. Requires activation.
CONCENTRATION: 40µg/ml (Pierce-BCA).
PURITY: ≥90% (SDS-PAGE, Western blot)
PURITY DETAIL: No other MMP contaminants are detectable.
FORMULATION: Liquid. In 50mM TRIS-HCl pH 7.0, containing 200mM NaCl, 5mM CaCl2, 1µM ZnCl2, 0.05% BRIJ35 0.05% sodium azide.
SPECIFIC ACTIVITY: ≥850mU/mg protein (Y. Masui, et al.; Biochem. Med. 17, 215 (1977)). One unit is defined as the amount of enzyme that hydrolyzes 1µmol Dnp-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min. at 37°C, pH 7.0.
APPLICATION: Immunogen for antibody generation, control in immunoassays and for characterizing interactions with MMP inhibitors.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Synthetic substrates for vertebrate collagenase: Y. Masui, et al.; Biochem. Med. 17, 215 (1977) Abstract
A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases: C.G. Knight, et al.; FEBS Lett. 296, 263 (1992) Abstract
Activation of progelatinase A and progelatinase A/TIMP-2 complex by membrane type 2-matrix metalloproteinase: H. Kolkenbrock, et al.; Biol. Chem. 378, 71 (1997) Abstract
General Information