ALEXIS Biochemicals: D2R (high purity)

ALX-260-094

Revised 09-May-06

D2R (high purity)
SYNONYMS	(L-Asp)₂ rhodamine 110 (high purity) . TFA salt
PRODUCT LINE	Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY	Caspase Substrates

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-260-094-M001		1 mg	120.00 USD
ALX-260-094-5001		5x1 mg	420.00 USD

Product Specification

FORMULA:	C₂₈H₂₄N₄O₉ . CF₃COOH
MW:	560.5 . 114.0
PURITY:	≥98%
APPEARANCE:	Off-white to orange powder.
SOLUBILITY:	Soluble in DMSO.
RECONSTITUTION:	Reconstitute in DMSO followed by the addition of ethanol at a ratio 1:1.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Aliquots in DMSO:ethanol (1:1) are stable for at least 1 month when stored at -20°C and stable for at least 6 months when stored at -80°C. Use high purity reagents only as the dye is instable in water. Discard surplus aqueous solutions.
HANDLING:	After reconstitution, prepare aliquots and store at -20°C. Protect from light.

Product Description

Substrate for detection of intracellular caspase activation by flow cytometry and fluorescence microscopy after apoptosis induction in intact hematopoietic cell lines. For detailed application protocols see [2].

Product Specific Literature References

[1] Rhodamine 110-linked amino acids and peptides as substrates to measure caspase activity upon apoptosis induction in intact cells: H. Hug, et al.; Biochemistry 38, 13906 (1999) Abstract
[2] Fluorogenic substrates as detectors of caspase activity during natural killer cell-induced apoptosis: M. Los, et al.; Methods Mol. Biol. 121, 155 (2000) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

D₂R can be used to detect caspase activity (predominantly caspase-3) in intact cells by flow cytometry [1], fluorescence microscopy as well as in cell extracts followed by analysis with a fluorometer (λex: 488nm/λem 550nm). Free (cleaved) Rhodamine 110 emits light both in the FL1 and FL2 channel. Dual staining is possible by employing the FL3 channel for flow cytometry. At least 160 assays can be performed (100µl sample volume (e.g. whole blood sample) and 100µM D₂R) [2]. Optimal substrate concentrations for other applications range from 25-50µM D₂R and have to be determined empirically for the particular cell system.

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