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ALX-620-056 Revised 17-Jul-08
DAF-2 DA (cell permeable)
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SYNONYMS 4,5-Diaminofluorescein . diacetate
PRODUCT LINE Nitric Oxide Pathway
PRODUCT CATEGORY Nitric Oxide & NOS Detection/Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-620-056-M001   1 mg 360.00 USD Add To Cart
Product Specification
FORMULA: C24H18N2O7
MW: 446.4
CONCENTRATION: ~5mM
PURITY: ≥99%
FORMULATION: Liquid. In 0.45ml DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY:

Prepare 500-fold dilution (~10µM) in phosphate buffer (0.1M phosphate, pH 7.4) immediately before use. BSA, phenol red and amines may affect the fluorescence and must be used with caution. Do not store diluted solutions.
HANDLING: Protect from light. After opening, prepare aliquots and store at -20°C. Keep under inert gas.

Product Images
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Product Description
Cell permeable analog of DAF-2 (Prod. No. ALX-620-052) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in nitric oxide (NO) levels in vivo (Ex(max): 495nm; Em(max): 515nm). Not for sale in Japan.
Product Specific Literature References
Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998) Abstract
Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins: H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) Abstract
Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins: N. Nakatsubo, et al.; FEBS Lett. 427, 263 (1998) Abstract
Development of a fluorescent indicator for nitric oxide based on the fluoroscein chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998) Abstract
Hypotonic stress-induced NO production in endothelium depends on endogenous ATP: C. Kimura, et al.; BBRC 274, 736 (2000) Abstract
Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells: T. Yoshioka, et al.; Br. J. Pharmacol. 129, 1530 (2000) Abstract
Fluroescent indicators for nitric oxide: H. Kojima & T. Nagano; Advanced Materials 12, 763 (2000)
Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A: J. Navarro-Antolin, et al.; FASEB J. 15, 1291 (2001) Abstract
Impairment of endothelial nitric oxide production by acute glucose overload: C. Kimura, et al.; Am. J. Physiol. Endocrinol. Metab. 280, E171 (2001) Abstract
Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging: M. Broillet, et al.; FEBS Lett. 491, 227 (2001) Abstract
Nitric Oxide Ameliorates Hydrophobic Bile Acid-induced Apoptosis in Isolated Rat Hepatocytes by Non-mitochondrial Pathways: E. Gumpricht, et al.; J. Biol. Chem. 277, 25823 (2002) Abstract; Full Text
BMP controls nitric oxide-mediated regulation of cell numbers in the developing neural tube: A. Traister, et al.; Cell Death Differ. (2004) Abstract
General Information
MANUFACTURER Manufactured by Daiichi Pure Chemicals Co. Ltd., Japan.
Further Categories Containing This Product:
Dyes/Stains/Fluorescent Probes/Fluorescent Labels
 
 
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