ALEXIS Biochemicals: DLL4 (human):Fc (human) (recombinant)

ALX-201-386

Revised 26-May-08

DLL4 (human):Fc (human) (recombinant)
SYNONYMS	Delta-like Protein 4 (human):Fc (human) (recombinant) Delta4 (human):Fc (human) (recombinant)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Delta-like [DLL] & Jagged/Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-386-C010		10 µg	310.00 USD
ALX-201-386-C050		50 µg	860.00 USD

Product Specification

SOURCE/HOST:	Produced in HEK 293 cells. Signal peptide and extracellular domain of recombinant human DLL4 (Delta-like protein 4; Delta4) (aa 1-529) is fused at the C-terminus to the Fc portion of human IgG1.
CONCENTRATION:
PURITY:	≥90% (SDS-PAGE)
FORMULATION:	Liquid. 0.2µm-filtered solution in PBS.
ENDOTOXIN CONTENT:	<0.1EU/µg protein (LAL-test).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Working aliquots are stable for up to 3 months when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

Product Images

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General Information

Notch signalling pathways play key roles in cell-fate determination and differentiation in many tissues during embryonic and postnatal development. Notch ligands are divided into two subclasses, the delta and the serrate family [1]. DLL4 (Dll4) is a novel delta family member and exhibits all the hallmarks of the delta family of Notch ligands [2]. Human DLL4 shares 86% aa sequence identity with mouse Dll4. The extracellular region of the 685 aa protein contains 8 EGF-like repeats as well as a DSL (Delta, Serrate, Lag2) domain necessary for receptor binding. The protein also contains a transmembrane domain and a cytoplasmic tail lacking any catalytic motifs. DLL4 is largely restricted to the vascular endothelium and has been shown to function as a ligand for Notch1 and Notch4 [3]. Signalling through Notch1 regulates formation of tip cells during angiogenesis [4].
BACKGROUND/TECHNICAL INFORMATION	Swiss-Prot link Q9NR61: DLL4 (human) [Precursor]
MANUFACTURER	Manufactured by AdipoGen, Inc.

General Literature References

[1] Human ligands of the Notch receptor: G.E. Gray, et al.; Am. J. Pathol. 154, 785 (1999) Abstract
[2] Dll4, a novel Notch ligand expressed in arterial endothelium: J.R. Shutter, et al.; Genes Dev. 14, 1313 (2000) Abstract
[3] Delta-like ligand 4 (Dll4) is induced by VEGF as a negative regulator of angiogenic sprouting: I.B. Lobov, et al.; PNAS 104, 3219 (2007) Abstract
[4] Dll4 signalling through Notch1 regulates formation of tip cells during angiogenesis: M. Hellstrom, et al.; Nature 445, 776 (2007) Abstract

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