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ALX-430-090 Revised 28-Aug-07
DMPO (high purity)
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SYNONYMS 5,5-Dimethyl-1-pyrroline-N-oxide (high purity)
2,2-Dimethyl-3,4-dihydro-2H-pyrrole 1-oxide (high purity)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Spin Traps, Spin Probes & Spin Labels
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-430-090-M500   500 mg 115.00 USD Add To Cart
ALX-430-090-G001   1 g 190.00 USD Add To Cart
Product Specification
FORMULA: C6H11NO
MW: 113.2
PURITY: ≥99% (GLC)
APPEARANCE: White crystalline to colorless liquid.
SOLUBILITY: Soluble in water, 100% ethanol, methanol or DMSO.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light and moisture. Hygroscopic.

Product Description
Cell permeable hydrophilic spin trap for both in vivo and in vitro studies of superoxide, O-, C-, S- and N- centered free radicals.
Product Specific Literature References
The spin trapping of superoxide and hydroxyl free radicals with DMPO (5,5-dimethylpyrroline-N-oxide): more about iron: G.R. Buettner; Free Radic. Res. Comm. 19 Suppl 1, S79 (1993) Abstract
Nitric oxide, superoxide and peroxynitrite: putative mediators of NMDA-induced cell death in cerebellar granule cells: M. Lafon-Cazal, et al.; Neuropharmacology 32, 1259 (1993) Abstract
Nitrone spin traps and a nitroxide antioxidant inhibit a common pathway of thymocyte apoptosis: A.F. Slater, et al.; Biochem. J. 306, 771 (1995) Abstract
Oxygen-centered spin adducts of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and 2H-imidazole 1-oxides: A.G. Krainev, et al.; J. Magn. Reson. B. 111, 272 (1996) Abstract
Formation of reactive oxygen species by pentaerithrityltetranitrate and glyceryl trinitrate in vitro and development of nitrate tolerance: S. Dikalov, et al.; J. Pharmacol. Exp. Ther. 286, 938 (1998) Abstract; Full Text
Formation of spin trap adducts during the decomposition of peroxynitrite: R.M. Gatti, et al.; Arch. Biochem. Biophys. 349, 36 (1998) Abstract
MDL 101,002, a free radical spin trap, is efficacious in permanent and transient focal ischemia models: M.P. Johnson, et al.; Life Sci. 63, 241 (1998) Abstract
Generation of nitric oxide from spin-trapping agents under oxidative conditions: K. Saito, et al.; Biosci. Biotech. Biochem. 62, 275 (1998)
Electron spin resonance detection of extracellular superoxide anion released by cultured endothelial cells: J.P. Souchard, et al.; Free Radic. Res. 29, 441 (1998) Abstract
Superoxide generation from endothelial nitric-oxide synthase. A Ca2+/calmodulin-dependent and tetrahydrobiopterin regulatory process: Y. Xia, et al.; J. Biol. Chem. 273, 25804 (1998) Abstract; Full Text
ESR measurement of rapid penetration of DMPO and DEPMPO spin traps through lipid bilayer membranes: K. Anzai, et al.; Arch. Biochem. Biophys. 415, 251 (2003) Abstract
Hydroxyl radical generation caused by the reaction of singlet oxygen with a spin trap, DMPO, increases significantly in the presence of biological reductants: C. Nishizawa, et al.; Free Radic. Res. 38, 385 (2004) Abstract
A new kinetic approach to the evaluation of rate constants for the spin trapping of superoxide/hydroperoxyl radical by nitrones in aqueous media: R. Lauricella, et al.; Org. Biomol. Chem. 2, 1304 (2004) Abstract
Superoxide radical anion adduct of 5,5-dimethyl-1-pyrroline n-oxide (DMPO). 1. The thermodynamics of formation and its acidity: F.A. Villamena, et al.; J. Phys. Chem. A. Mol. Spectrosc. Kinet. Environ. Gen. Theory 109, 6083 (2005) Abstract
Superoxide radical anion adduct of 5,5-dimethyl-1-pyrroline N-oxide (DMPO). 2. The thermodynamics of decay and EPR spectral properties: F.A. Villamena, et al.; J. Phys. Chem. A. Mol. Spectrosc. Kinet. Environ. Gen. Theory 109, 6089 (2005) Abstract
Characterization of the high-resolution ESR spectra of superoxide radical adducts of 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide (DEPMPO) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Analysis of conformational exchange: S. Dikalov, et al.; Free Radic. Res. 39, 825 (2005) Abstract
Assignment of the EPR spectrum of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) superoxide spin adduct: J.L. Clement, et al.; J. Org. Chem. 70, 1198 (2005) Abstract
Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species: H. Shi, et al.; Arch. Biochem. Biophys. 437, 59 (2005) Abstract
Nitric oxide decreases the stability of DMPO spin adducts: K.J. Reszka, et al.; Nitric Oxide 15, 133 (2006) Abstract
Immunolocalization of hypochlorite-induced, catalase-bound free radical formation in mouse hepatocytes: M.G. Bonini et al.; Free Rad. Biol. Med. 42, 530 (2007)
 
 

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