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DNA & RNA Methyl & Alkyl Transferases / Related Products
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ALX-480-096 Revised 18-Aug-08
5-Aza-2'-deoxycytidine
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SYNONYMS 5-Aza-CdR
5-Aza-dC
5-Deoxy-2'-azacytidine
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY Nucleobases
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ALX-480-096-M005   5 mg 240.00 USD Add To Cart
Product Specification
FORMULA: C8H12N4O4
MW: 228.2
CAS NUMBER: 2353-33-5
RTECS: XZ3012000
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: 25mg/ml soluble in DMSO or 50% acetic acid; 1mg/ml soluble in methanol. (Warming may be required to achieve complete solubilization.)
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Packaged under inert gas. After reconstitution, prepare aliquots and store at -20°C.
HAZARD: HARMFUL.

Product Description
Inhibitor of DNA methyltransferase. Restores mRNA and protein expression of caspase-8 and TRAIL (tumor necrosis factor-related apoptosis inducing ligand) sensitivity of resistant cell lines. Enhances apoptosis induced by HDAC (histone deacetylase) inhibitors.
Product Specific Literature References
Incorporation of a potent antileukemic agent, 5-aza-2'-deoxycytidine, into DNA of cells from leukemic mice: J. Vesely and A. Cihak; Cancer Res. 37, 3684 (1977) Abstract
Cellular differentiation, cytidine analogs and DNA methylation: P.A. Jones and S.M. Taylor; Cell 20, 85 (1980) Abstract
The effects of 5-azacytidine and 5-azadeoxycytidine on chromosome structure and function: implications for methylation-associated cellular processes: T. Haaf; Pharmacol. Ther. 65, 19 (1995) Abstract
Loss of caspase-8 expression in highly malignant human neuroblastoma cells correlates with resistance to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis: S. Hopkins-Donaldson, et al.; Cancer Res. 60, 4315 (2000) Abstract; Full Text
Resistance to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in neuroblastoma cells correlates with a loss of caspase-8 expression: A. Eggert, et al.; Cancer Res. 61, 1314 (2001) Abstract; Full Text
5-Aza-2'-deoxycytidine induces histone hyperacetylation of mouse centromeric heterochromatin by a mechanism independent of DNA demethylation: S. Takebayashi, et al.; BBRC 288, 921 (2001) Abstract
DNA methyltransferase inhibition enhances apoptosis induced by histone deacetylase inhibitors: W.G. Zhu, et al.; Cancer Res. 61, 1327 (2001) Abstract; Full Text
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DNA & RNA Methyl & Alkyl Transferases / Related Products
 
 
ALX-480-019 Revised 17-Nov-08
O6-Benzylguanine
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA & RNA Methyl & Alkyl Transferases / Related Products
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ALX-480-019-M010   10 mg 30.00 USD Add To Cart
ALX-480-019-M050   50 mg 95.00 USD Add To Cart
Product Specification
FORMULA: C12H11N5O
MW: 241.2
CAS NUMBER: 19916-73-5
PURITY: ≥99%
APPEARANCE: White to off-white powder.
SOLUBILITY: Soluble in methanol (20mg/ml).
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HAZARD: IRRITANT.
IDENTITY: Identity determined by 1H-NMR.

Product Description
Effective substrate and efficient inactivator of DNA alkyltransferase.
Product Specific Literature References
Activation of human O6-alkylguanine-DNA alkyltransferase by DNA: K. Goodtzova, et al.; Biochemistry 33, 8385 (1994) Abstract
Differential inactivation of mammalian and Escherichia coli O6-alkylguanine-DNA alkyltransferases by O6-benzylguanine: R.H. Elder, et al.; Biochem. J. 298, 231 (1994) Abstract
O6-benzylguanine and its role in chemotherapy: M.E. Dolan & A.E. Pegg; Cancer Res. 3, 837 (1997) Abstract
Prolonged inhibition of O(6)-methylguanine DNA methyltransferase in human tumor cells by O(6)-benzylguanine in vitro and in vivo: E.L. Kreklau, et al.; Pharmacol. Exp. Ther. 291, 1269 (1999) Abstract
 
 
ALX-380-242 Revised 03-Apr-08
Chaetocin (high purity)
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PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antitumor Antibiotics
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ALX-380-242-M001   1 mg 330.00 USD Add To Cart
Product Specification
FORMULA: C30H28N6O6S4
MW: 696.9
CAS NUMBER: 28097-03-2
RTECS: FM3032000
SOURCE/HOST: Isolated from Chaetomium sp. MST-FP2085.
PURITY: ≥99% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, dimethyl formamide, methanol or 100% ethanol; poorly soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HAZARD: HARMFUL.

Product Description
Antitumor antibiotic. Specific inhibitor of the lysine-specific methyltransferase SU(VAR)3-9 both in vitro and in vivo.
Product Specific Literature References
Isolation and configuration of Chaetocin: D. Hauser, et al.; Helv. Chim. Acta. 53, 1061 (1970)
Identification of a specific inhibitor of the histone methyltransferase SU(VAR)3-9: D. Greiner, et al.; Nat. Chem. Biol. 1, 143 (2005) Abstract
Chaetocin: a promising new antimyeloma agent with in vitro and in vivo activity mediated via imposition of oxidative stress: C.R. Isham, et al.; Blood 109, 2579 (2007) Abstract
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DNA & RNA Methyl & Alkyl Transferases / Related Products
 
 
ALX-550-516 Revised 21-May-08
Compound A
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SYNONYMS CpdA
2-((4-Acetophenyl)-2-chloro-N-methyl)ethylammonium chloride
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
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ALX-550-516-M005   5 mg 40.00 USD Add To Cart
ALX-550-516-M025   25 mg 120.00 USD Add To Cart
Product Specification
FORMULA: C11H14ClNO2 . HCl
MW: 227.7 . 36.5
PURITY: ≥98% (HPLC)
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or water.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: Stock solutions in ethanol are stable for up to 1 month when stored at -80°C.
HANDLING: Protect from moisture. After reconstitution, prepare aliquots and store at -80°C.

Product Description
Cell permeable, nonsteroidal glucocorticoid receptor modulator with high binding affinity. Exerts anti-inflammatory potential by down-modulating TNF-induced pro-inflammatory gene expression, such as IL-6 and E-selectin. Unlike dexamethasone (Prod. No. ALX-370-002) it does not enhance glucocorticoid response element (GRE)-driven gene expression or induce glucocorticoid receptor (GR) binding to GRE-dependent genes in vivo.
Product Specific Literature References
A fully dissociated compound of plant origin for inflammatory gene repression: K. De Bosscher, et al.; PNAS 102, 15827 (2005) Abstract
A plant-derived ligand favoring monomeric glucocorticoid receptor conformation with impaired transactivation potential attenuates collagen-induced arthritis: P. Dewint, et al.; J. Immunol. 180, 2608 (2008) Abstract
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Further Categories Containing This Product:
GITR & GITRL Other Products • Glucocorticoids & Glucocorticoid Receptors / Related Products • RANK & RANKL / OPG Other Products • Natural Products - Nitric Oxide Pathway Modulators • DNA & RNA Methyl & Alkyl Transferases / Related Products • Antitumor Agents (Hormone-related) • NOS Inhibitors (NOS Induction & Enzyme Activity) • Natural Products - Chemopreventive Agents • Other Natural Products - DNA Regulation / Transcription • Natural Products for Cell Cycle Research
 
 
ALX-370-002 Revised 19-Sep-06
Dexamethasone
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SYNONYMS 9α-Fluoro-16α-methylprednisolone
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Glucocorticoids & Glucocorticoid Receptors / Related Products
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ALX-370-002-M250   250 mg 22.00 USD Add To Cart
ALX-370-002-G001   1 g 45.00 USD Add To Cart
Product Specification
FORMULA: C22H29FO5
MW: 392.5
CAS NUMBER: 50-02-2
MERCK INDEX: 14: 2943
RTECS: TU3980000
PURITY: ≥98%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in DMSO, 100% ethanol or methanol. Slightly soluble in acetone or chloroform. Insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Protect from light.
HAZARD: HARMFUL. MAY BE TERATOGENIC.

Product Description
Inhibits constitutive OPG mRNA steady-state levels in a dose- and time-dependent fashion, promoting osteoclast formation. Glucocorticoid with anti-inflammatory activity. Inhibits the induction of nitric oxide synthases (NOS).
Product Specific Literature References
Inhibition of the induction of nitric oxide synthase by glucocorticoids: yet another explanation for their anti-inflammatory effects?: S. Moncada & R.M.J. Palmer; TIPS 12, 130 (1991), (Review) Abstract
Glucocorticoids inhibit the induction of nitric oxide synthase and the related cell damage in adenocarcinoma cells: K.J. O'Connor & S. Moncada; Biochim. Biophys. Acta 1097, 227 (1991) Abstract
Nitric oxide mediates tumor necrosis factor-alpha cytotoxicity in endothelial cells: C. Estrada, et al.; BBRC 186, 475 (1992) Abstract
Dexamethasone inhibits the expression of an inducible nitric oxide synthase in infarcted rabbit myocardium: R.R. Dudek, et al.; BBRC 202, 1120 (1994) Abstract
Induction of the alkyltransferase (MGMT) gene by DNA damaging agents and the glucocorticoid dexamethasone and comparison with the response of base excision repair genes: T. Grombacher, et al.; Carcinogenesis 17, 2329 (1996) Abstract
Activation of human O6-methylguanine-DNA methyltransferase gene by glucocorticoid hormone: T. Biswas, et al.; Oncogene 18, 525 (1999) Abstract
Dexamethasone-induced thymocyte apoptosis: apoptotic signal involves the sequential activation of phosphoinositide-specific phospholipase C, acidic sphingomyelinase, and caspases: M.G. Cifone, et al.; Blood 93, 2282 (1999) Abstract; Full Text
Dexamethasone induces apoptosis in human T cell clones expressing low levels of Bcl-2: M.S. Montani, et al.; Cell Death Differ 6, 79 (1999) Abstract
Dexamethasone inhibits the phosphorylation of retinoblastoma protein in the suppression of human vascular smooth muscle cell proliferation: T.D. Reil, et al.; J. Surg. Res. 92, 108 (2000) Abstract
Dexamethasone suppresses iNOS gene expression by inhibiting NF-kappaB in vascular smooth muscle cells: M. Matsumura, et al.; Life Sci. 69, 1067 (2001) Abstract
Dexamethasone inhibits inducible nitric-oxide synthase expression and nitric oxide production by destabilizing mRNA in lipopolysaccharide-treated macrophages: R. Korhonen, et al.; Mol. Pharmacol. 62, 698 (2002) Abstract; Full Text
Molecular mechanisms underlying dexamethasone inhibition of iNOS expression and activity in C6 glioma cells: J. Shinoda, et al.; Glia 42, 68 (2003) Abstract
Dexamethasone prevents granulocyte-macrophage colony-stimulating factor-induced nuclear factor-kappaB activation, inducible nitric oxide synthase expression and nitric oxide production in a skin dendritic cell line: A.L. Vital, et al.; Mediators Inflamm. 12, 71 (2003) Abstract
Dexamethasone suppresses eNOS and CAT-1 and induces oxidative stress in mouse resistance arterioles: S.C. Schafer, et al.; Am. J. Physiol. Heart Circ. Physiol. 288, H436 (2005) Abstract; Full Text
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Further Categories Containing This Product:
GITR & GITRL Other Products • RANK & RANKL / OPG Other Products • Antitumor Agents (Hormone-related) • NOS Inhibitors (NOS Induction & Enzyme Activity) • Chemopreventive Agents Other Products • DNA & RNA Methyl & Alkyl Transferases / Related Products • Cell Cycle Other Products
 
 
ALX-201-065 Revised 18-Sep-08
MGMT (human) (recombinant)
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SYNONYMS O6-Methylguanine-DNA Methyltransferase (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Repair Other Products
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ALX-201-065-R050   50 µl 320.00 USD Add To Cart
Product Specification
EC: 2.1.1.63
SOURCE/HOST: Produced in E. coli. Full length human MGMT is fused at the N-terminus to a GST-tag.
PURITY: Purified with Glutathiose-Sepharose 4B and thrombin cleavage
FORMULATION: Liquid. 5'000U in 50µl buffer containing 50% glycerol, 100µg/ml BSA, 20mM TRIS-HCl, pH 7.8, 5mM dithiothreitol, 60mM KCl and 1mM EDTA.
SPECIFIC ACTIVITY: 100U/µl. One unit is defined as the amount of enzyme that removes 100fmol O6-methylguanine from DNA.
QUALITY CONTROL: The biological activity has been checked in a radioactive standard MGMT assay.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Do not store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P16455: MGMT (human)
Further Categories Containing This Product:
DNA & RNA Methyl & Alkyl Transferases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-804-369 Revised 13-Nov-08
Monoclonal Antibody to Dnmt1 (60B1220.1)
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SYNONYMS anti-DNA Methyltransferase-1 MAb (60B1220.1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA & RNA Methyl & Alkyl Transferases / Related Products
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ALX-804-369-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 60B1220.1
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 637-650 of human Dnmt1 (DNA methyltransferase-1).
SPECIFICITY: Recognizes human, mouse and zebrafish Dnmt1.
APPLICATION: Immunocytochemistry (see [14])
Immunohistochemistry (1-2µg/ml) (paraffin sections (see [17]))
Immunoprecipitation (see [3,7]), Chromatin Immunoprecipitation (see [11]))
Western Blot (2-4µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
[1] Lsh, a member of the SNF2 family, is required for genome-wide methylation: K. Dennis, et al.; Genes Dev. 15, 2940 (2001) Abstract; Full Text
[2] The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription: R. Santoro, et al.; Nat. Genet. 32, 393 (2002) Abstract
[3] Farnesyltransferase inhibitor (L-744,832) restores TGF-beta type II receptor expression and enhances radiation sensitivity in K-ras mutant pancreatic cancer cell line MIA PaCa-2: R.A. Alcock, et al.; Oncogene 21, 7883 (2002) Abstract
[4] The Epstein-Barr virus oncogene product, latent membrane protein 1, induces the downregulation of E-cadherin gene expression via activation of DNA methyltransferases: C.N. Tsai, et al.; PNAS 99, 10084 (2002) Abstract; Full Text
[5] Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter: Z. Zhang, et al.; Mech. Ageing Dev. 123, 1257 (2002) Abstract
[6] Double RNA interference of DNMT3b and DNMT1 enhances DNA demethylation and gene reactivation: Y.W. Leu, et al.; Cancer Res. 63, 6110 (2003) Abstract
[7] Decrease of DNA methyltransferase 1 expression relative to cell proliferation in transitional cell carcinoma: F. Kimura, et al.; Int. J. Cancer 104, 568 (2003) Abstract
[8] Analysis of mammalian proteins involved in chromatin modification reveals new metaphase centromeric proteins and distinct chromosomal distribution patterns: J.M. Craig, et al.; Hum. Mol. Genet. 12, 3109 (2003) Abstract; Full Text
[9] Components of a pathway maintaining histone modification and heterochromatin protein 1 binding at the pericentric heterochromatin in Mammalian cells: H. Xin, et al.; J. Biol. Chem. 279, 9539 (2004) Abstract; Full Text
[10] Induction of DNA methylation and gene silencing by short interfering RNAs in human cells: H. Kawasaki and K. Taira; Nature 431, 211 (2004) Abstract
[11] Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004) Abstract
[12] RNA interference-mediated knockdown of DNA methyltransferase 1 leads to promoter demethylation and gene re-expression in human lung and breast cancer cells: M. Suzuki, et al.; Cancer Res. 64, 3137 (2004) Abstract; Full Text
[13] Deregulation of DNA methyltransferases and loss of parental methylation at the insulin-like growth factor II (Igf2)/H19 loci in p53 knockout mice prior to tumor development: I.Y. Park, et al.; J. Cell Biochem. 94, 585 (2005) Abstract
[14] 5-Aza-deoxycytidine induces selective degradation of DNA methyltransferase 1 by a proteasomal pathway that requires the KEN box, bromo-adjacent homology domain, and nuclear localization signal: K. Ghoshal, et al.; Mol. Cell. Biol. 25, 4727 (2005) Abstract
[15] Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma: B.I. Srivastava and M.D. Srivastava; Leuk. Res. 29, 771 (2005) Abstract
[16] Immunology of cutaneous vasculitis associated with both etanercept and infliximab: M.D. Srivastava, et al.; Scand. J. Immunol. 61, 329 (2005) Abstract
[17] An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection: T.S. Lin, et al.; Cancer Lett. 226, 77 (2005) Abstract
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Monoclonal Antibodies
 
 
ALX-804-370 Revised 14-Feb-08
Monoclonal Antibody to Dnmt3a (mouse) (64B1446)
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SYNONYMS anti-DNA Methyltransferase-3a MAb (mouse) (64B1446)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA & RNA Methyl & Alkyl Transferases / Related Products
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ALX-804-370-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
CLONE: 64B1446
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant mouse Dnmt3a (DNA methyltransferase-3a).
SPECIFICITY: Recognizes C-terminal epitope aa 705-908 of mouse Dnmt3a [1]. Weakly cross-reacts with human Dnmt3a.
APPLICATION: Immunocytochemistry (5µg/ml)
Immunohistochemistry (1:400) (see [8])
Immunoprecipitation, Chromatin Immunoprecipitation (see [4])
Western Blot (2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
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Product Specific Literature References
[1] Lsh, a member of the SNF2 family, is required for genome-wide methylation: K. Dennis, et al.; Genes Dev. 15, 2940 (2001) Abstract; Full Text
[2] A novel Dnmt3a isoform produced from an alternative promoter localizes to euchromatin and its expression correlates with active de novo methylation: T. Chen, et al.; J. Biol. Chem. 277, 38746 (2002) Abstract
[3] Association of Lsh, a regulator of DNA methylation, with pericentromeric heterochromatin is dependent on intact heterochromatin: Q. Yan, et al.; Mol. Cell. Biol. 23, 8416 (2003) Abstract; Full Text
[4] Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004) Abstract
[5] De novo DNA methyltransferases Dnmt3a and Dnmt3b primarily mediate the cytotoxic effect of 5-aza-2’-deoxycytidine: M. Oka, et al.; Oncogene 24, 3091 (2005) Abstract
[6] Myc represses transcription through recruitment of DNA methyltransferase corepressor: C. Brenner, et al.; EMBO 24, 336 (2005) Abstract
[7] Cell and stage of transformation-specific effects of folate deficiency on methionine cycle intermediates and DNA methylation in an in vitro model: J.M. Stempak, et al.; Carcinogenesis 26, 981 (2005) Abstract
[8] Dynamic expression of de novo DNA methyltransferases Dnmt3a and Dnmt3b in the central nervous system: J. Feng, et al.; J. Neurosci. Res. 79, 734 (2005) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-233 Revised 31-May-07
Monoclonal Antibody to Dnmt3b (52A1018)
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SYNONYMS anti-DNA Methyltransferase-3b MAb (52A1018)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA & RNA Methyl & Alkyl Transferases / Related Products
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ALX-804-233-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 52A1018
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.2% gelatin and 0.05% sodium azide.
IMMUNOGEN: Recombinant mouse Dnmt3b (DNA methyltransferase-3b).
SPECIFICITY: Recognizes human and mouse Dnmt3b. Detects a band of ~100kDa by Western blot. Does not cross-react with Dnmt3a.
APPLICATION: Immunocytochemistry (1-2µg/ml)
Immunohistochemistry (see [6] and [7])
Immunoprecipitation (1-2µg/ml); Chromatin Immunoprecipitation (see [5])
Western Blot (2-4µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
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Product Specific Literature References
[1] The nucleolar remodeling complex NoRC mediates heterochromatin formation and silencing of ribosomal gene transcription: R. Santoro, et al.; Nat. Genet. 32, 393 (2002) Abstract
[2] Double RNA interference of DNMT3b and DNMT1 enhances DNA demethylation and gene reactivation: Y.W. Leu, et al.; Cancer Res. 63, 6110 (2003) Abstract
[3] Role of the DNA methyltransferase variant DNMT3b3 in DNA methylation: D.J. Weisenberger, et al.; Mol. Cancer Res. 2, 62 (2004) Abstract; Full Text
[4] Induction of DNA methylation and gene silencing by short interfering RNAs in human cells: H. Kawasaki and K. Taira; Nature 431, 211 (2004) Abstract
[5] Loss of estrogen receptor signaling triggers epigenetic silencing of downstream targets in breast cancer: Y.W. Leu, et al.; Cancer Res. 64, 8184 (2004) Abstract
[6] An association of DNMT3b protein expression with P16INK4a promoter hypermethylation in non-smoking female lung cancer with human papillomavirus infection: T.S. Lin, et al.; Cancer Lett. 226, 77 (2005) Abstract
[7] Stage-specific induction of DNA methyltransferases in olfactory receptor neuron development: J.L. MacDonald, et al.; Dev. Biol. 288, 461 (2005) Abstract; Full Text
[8] Reduced genomic cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein: D.L. Carlone, et al.; Mol. Cell. Biol. 25, 4881 (2005) Abstract; Full Text
General Information
DNA methylation is an important regulator of genetic information, shutting down gene expression through the addition of methyl groups to cytosine (C) bases in the DNA. The establishment of DNA methylation patterns requires de novo methylation that occurs predominantly during early development and gametogenesis. DNA methyltransferase-1 (Dnmt1) "maintains" C methylation after DNA replication by methylating the daughter DNA strand using the methylation pattern of the parental strand as a template. Dnmt3a and Dnmt3b are essential for the de novo methylation. They show nonoverlapping functions in development, with Dnmt3b specifically required for methylation of centromeric minor satellite repeats. Both Dnmt3a and Dnmt3b expression is elevated in several tumor cell lines comparable to Dnmt1. It has also been suggested that mutations of Dnmt3b are responsible for the ICF immunodeficiency syndrome.
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Monoclonal Antibodies
 
 
ALX-804-567 Revised 13-Dec-04
Monoclonal Antibody to SET7/9 (human) (s4E5)
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA & RNA Methyl & Alkyl Transferases / Related Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-567-R050   50 µl 195.00 USD Add To Cart
ALX-804-567-R100   100 µl 320.00 USD Add To Cart
Product Specification