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Transcription Factors / Related Products
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ALX-201-094 Revised 03-May-06
Caspase-5 (active) (human) (recombinant)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspases
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-094-U025   25 U 203.00 USD Add To Cart
ALX-201-094-U100   100 U 371.00 USD Add To Cart
Product Specification
EC: 3.4.22.-
SOURCE/HOST: Produced in E. coli.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Lyophilized.
RECONSTITUTION: Reconstitute to 1U/µl with PBS containing 15% glycerol.
SPECIFIC ACTIVITY: ~5'000U/mg protein. One unit is defined as the amount of enzyme that cleaves 1nmol of the caspase substrate WEHD-pNA per hour at 37°C in a reaction solution containing 50mM HEPES, pH 7.2, 50mM NaCl, 0.1% CHAPS, 10mM EDTA, 5% glycerol and 10mM DTT.
APPLICATION: In combination with caspase-5 activity assays, the enzyme is useful in screening caspase inhibitors, studying enzyme regulation and kinetics, determining target substrate or as a positive control in caspase-5 activity assays. We recommend using 1 unit per assay for analyzing caspase activity.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P51878: Caspase-5 (human) (precursor)
AfCS Signalling Gateway link A000500: Caspase-5 (mouse)
Further Categories Containing This Product:
InflammasomeEnzymesRecombinant Proteins / Fusion Proteins
 
 
ALX-850-217 Revised 24-Jan-05
Caspase-5 Colorimetric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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ALX-850-217-KI01   25 tests 203.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-pNA Substrate (4mM), 100µl DTT (1M), 25ml Dilution Buffer.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Store Cell Lysis Buffer, 2X Reaction Buffer and Dilution Buffer at 4°C after opening.
Product Description
Based on the spectrophotometric detection of the chromophore pNA (p-nitroaniline) after cleavage from the labelled substrate WEHD-pNA.
General Information
The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405nm. Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
InflammasomeActivity Assays
 
 
ALX-850-218 Revised 24-Jan-05
Caspase-5 Fluorometric Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Kits & Sets
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ALX-850-218-KI01   25 tests 189.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: 25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125µl WEHD-AFC Substrate (1mM), 100µl DTT (1M).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Store Cell Lysis Buffer and 2X Reaction Buffer at 4°C after opening.
Product Description
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate WEHD-AFC.
General Information
WEHD-AFC emits blue light (λmax = 400nm); upon cleavage of the substrate by caspase-5 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from a treated sample with an untreated control allows determination of the fold increase in caspase-5 activity.
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
InflammasomeActivity Assays
 
 
ALX-201-199 Revised 11-Jan-07
CEBP-α (270-358) (human) (recombinant) (His)
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SYNONYMS CCAAT/Enhancer Binding Protein α (270-358) (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Transcription Factors Other Products
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ALX-201-199-C100   100 µg 220.00 USD Add To Cart
Product Specification
MW: ~14.5kDa.
SOURCE/HOST: Produced in E. coli. bZip region of CEBP-α (aa 270-358).
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In TRIS-HCl, pH 7.5, containing 0.1M NaCl and 5mM β-mercaptoethanol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-201-200 Revised 04-Mar-05
CEBP-γ (39-147) (human) (recombinant) (His)
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SYNONYMS CCAAT/Enhancer Binding Protein γ (39-147) (human) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Transcription Factors Other Products
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ALX-201-200-C100   100 µg 220.00 USD Add To Cart
Product Specification
MW: ~16.5kDa.
SOURCE/HOST: Produced in E. coli. DNA binding domain of CEBP-γ (aa 39-147).
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In TRIS-HCl, pH 7.5, containing 0.1M NaCl and 5mM β-mercaptoethanol.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
 
 
ALX-350-332 Revised 20-Oct-08
Celastrol
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SYNONYMS Tripterine
3-Hydroxy-24-nor-2-oxo-1(10),3,5,7-friedelatetraen-29-oic acid
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
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ALX-350-332-M005   5 mg 65.00 USD Add To Cart
ALX-350-332-M025   25 mg 260.00 USD Add To Cart
Product Specification
FORMULA: C29H38O4
MW: 450.6
CAS NUMBER: 34157-83-0
SOURCE/HOST: Isolated from the root of Tripterygium sp.
PURITY: ≥98% (HPLC)
APPEARANCE: Red cubic crystals.
SOLUBILITY: Soluble in 100% ethanol, DMSO or dimethyl formamide; insoluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.

Product Description
Cell permeable triterpenoid; anti-inflammatory and immunosuppressive compound. Antioxidant. Suppresses LPS-induced cytokine release in macrophages and monocytes (IC50=40nM for IL-1β and IL-1α, 80nM for IL-6, 110nM for prostaglandin E2 and 210nM for IL-8 and TNF-α). Suppresses nitric oxide (NO) production (IC50=230nM) and LPS-induced NF-κB activation (IC50=270nM). Inhibits chymotrypsin-like activity of 20S proteasome (IC50=2.5µM). Inhibits lipid peroxidation induced by ADP and Fe2+ in rat liver mitochondria (IC50=7µM). Induces expression of a wider set of heat shock proteins. Inhibits topoisomerse II.
Product Specific Literature References
The triterpene celastrol as a very potent inhibitor of lipid peroxidation in mitochondria: H. Sassa, et al.; BBRC 172, 890 (1990) Abstract
Structural basis of potent antiperoxidation activity of the triterpene celastrol in mitochondria: effect of negative membrane surface charge on lipid peroxidation: H. Sassa, et al.; Free Radic. Biol. Med. 17, 201 (1994) Abstract
Novel cytokine release inhibitors. Part III: Truncated analogs of tripterine: W. He, et al.; Bioorg. Med. Chem. Lett. 8, 3659 (1998) Abstract
Celastrol, a potent antioxidant and anti-inflammatory drug, as a possible treatment for Alzheimer’s disease: A.C. Allison, et al.; Prog. Neuropsychopharmacol. Biol. Psychiatry 25, 1341 (2001) Abstract
Antiinflammatory constituents of Celastrus orbiculatus inhibit the NF-kappaB activation and NO production: H.Z. Jin, et al.; J. Nat. Prod. 65, 89 (2002) Abstract
Apoptosis induction in HL-60 cells and inhibition of topoisomerase II by triterpene celastrol: M. Nagase, et al.; Biosci. Biotechnol. Biochem. 67, 1883 (2003) Abstract; Full Text
Celastrol inhibits pro-inflammatory cytokine secretion in Crohn’s disease biopsies: G.F. Pinna, et al.; BBRC 322, 778 (2004) Abstract
Celastrols as inducers of the heat shock response and cytoprotection: S.D. Westerheide, et al.; J. Biol. Chem. 279, 56053 (2004) Abstract; Full Text
Celastrol protects against MPTP- and 3-nitropropionic acid-induced neurotoxicity: C. Cleren, et al.; J. Neurochem. 94, 995 (2005) Abstract
Celastrol blocks neuronal cell death and extends life in transgenic mouse model of amyotrophic lateral sclerosis: M. Kiaei, et al.; Neurodegener. Dis. 2, 246 (2005) Abstract
Celastrol, a triterpene extracted from the Chinese "Thunder of God Vine," is a potent proteasome inhibitor and suppresses human prostate cancer growth in nude mice: H. Yang, et al.; Cancer Res. 66, 4758 (2006) Abstract; Full Text
Celastrol, a novel triterpene, potentiates TNF-induced apoptosis and suppresses invasion of tumor cells by inhibiting NF-kappaB-regulated gene products and TAK1-mediated NF-kappaB activation: G. Sethi, et al.; Blood 109, 2727 (2007) Abstract
Induction of heat shock proteins in differentiated human and rodent neurons by celastrol: A.M. Chow & I.R. Brown; Cell Stress Chaperones 12, 237 (2007) Abstract
 
 
ALX-201-279 Revised 18-Mar-08
CREB Binding Protein (mouse) (recombinant) (His)
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SYNONYMS CBP (mouse) (recombinant) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY CREB & CREB Binding Protein / Related Products
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ALX-201-279-C002   2 µg 280.00 USD Add To Cart
Product Specification
EC: EC 2.3.1.48
SOURCE/HOST: Produced in SF21 cells. Fused to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol.
SPECIFIC ACTIVITY: ~50-100ng are required for standard HAT assay.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store in liquid nitrogen.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P45481: CREB Binding Protein (mouse)
 
 
ALX-350-028 Revised 12-Sep-08
Curcumin (high purity)
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SYNONYMS 1,7-bis(4-Hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione
Diferuloylmethane
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Natural Products - Anti-inflammatory Agents
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-350-028-M010   10 mg 15.00 USD Add To Cart
ALX-350-028-M050   50 mg 60.00 USD Add To Cart
ALX-350-028-M250   250 mg 240.00 USD Add To Cart
Product Specification
FORMULA: C21H20O6
MW: 368.4
CAS NUMBER: 458-37-7
MERCK INDEX: 14: 2673
SOURCE/HOST: Isolated from turmeric (Curcuma longa).
PURITY: ≥98.5% (Note: This highly purified product is free of demethoxy- and bis-demethoxycurcumin and does not contain 30-40% bioactive impurities)
APPEARANCE: Orange-yellow crystalline powder.
SOLUBILITY: Soluble in acetic acid or 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +20°C
HANDLING: Protect from light.

Product Description
Dual inhibitor of cyclooxygenase (COX) and 5-lipoxygenase. Also inhibits glutathione S-transferase, induction of nitric oxide (NO) in activated macrophages and inhibits EGF-induced tyrosine phosphorylation of EGF receptors. Antioxidant. Inhibitor of NAD(P)H:quinone oxidoreductase 1 (NQO1), disrupting the binding of NQO1 to wild type p53 inducing ubiquitin-independent degradation of p53 and inhibits p53-mediated apoptosis in normal thymocytes and myeloid leukemic cells.
Product Specific Literature References
Modification of certain inflammation-induced biochemical changes by curcumin: R. Srivastava & R.C. Srimal; Indian J. Med. Res. 81, 215 (1985) Abstract
Inhibition of 5-hydroxy-eicosatetraenoic acid (5-HETE) formation in intact human neutrophils by naturally-occurring diarylheptanoids: inhibitory activities of curcuminoids and yakuchinones: D.L. Flynn, et al.; Prostagl. Leukotr. Med. 22, 357 (1986) Abstract
Inhibitory effect of curcumin on epidermal growth factor receptor kinase activity in A431 cells: L. Korutla & R. Kumar; Biochim. Biophys. Acta 1224, 597 (1994) Abstract
Curcumin, an anti-tumour promoter and anti-inflammatory agent, inhibits induction of nitric oxide synthase in activated macrophages: I. Brouet & H. Okshima; BBRC 206, 533 (1995) Abstract
Cytotoxicity and cytoprotective activities of natural compounds. The case of curcumin: J.N. Commandeur & N.P. Vermeulen; Xenobiotica 26, 667 (1996) Abstract
Nitric oxide scavenging by curcuminoids: Sreejayan & M.N. Rao; J. Pharm. Pharmacol. 49, 105 (1997) Abstract
Inhibitory effects of curcumin on tumorigenesis in mice: M.T. Huang, et al.; J. Cell. Biochem. (Suppl.) 27, 26 (1997) Abstract
In vivo inhibition of nitric oxide synthase gene expression by curcumin, a cancer preventive natural product with anti-inflammatory properties: M.M. Chan, et al.; Biochem. Pharmacol. 55, 1955 (1998) Abstract
Effect of curcumin on the production of nitric oxide by cultured rat mammary gland: M. Onoda & H. Inano; Nitric Oxide 4, 505 (2000) Abstract
Suppression of nitric oxide oxidation to nitrite by curcumin is due to the sequestration of the reaction intermediate nitrogen dioxide, not nitric oxide: B.D. Johnston & E.G. DeMaster; Nitric Oxide 8, 231 (2003) Abstract
Biological properties of curcumin-cellular and molecular mechanisms of action: B. Joe, et al.; Crit. Rev. Food Sci. Nutr. 44, 97 (2004), (Review) Abstract
Curcumin, a major constituent of turmeric, corrects cystic fibrosis defects: M.E. Egan, et al.; Science 304, 600 (2004) Abstract
Inhibition of NAD(P)H:quinone oxidoreductase 1 activity and induction of p53 degradation by the natural phenolic compound curcumin: P. Tsvetkov, et al.; PNAS 102, 5535 (2005) Abstract
Immunomodulatory effects of curcumin: V.S. Yadav, et al.; Immunopharmacol. Immunotoxicol. 27, 485 (2005) Abstract
Curcumin, an atoxic antioxidant and natural NFkappaB, cyclooxygenase-2, lipooxygenase, and inducible nitric oxide synthase inhibitor: a shield against acute and chronic diseases: S. Bengmark; JPEN J. Parenter. Enteral. Nutr. 30, 45 (2006), (Review) Abstract
Curcumin is an inhibitor of p300 histone acetylatransferase: M.G. Marcu, et al.; Med. Chem. 2, 169 (2006) Abstract
Multiple biological activities of curcumin: a short review: R.K. Maheshwari, et al.; Life Sci. 78, 2081 (2006), (Review) Abstract
Curcumin inhibits hypoxia-induced angiogenesis via down-regulation of HIF-1: M.K. Bae, et al.; Oncol. Rep. 15, 1557 (2006) Abstract
Notch-1 down-regulation by curcumin is associated with the inhibition of cell growth and the induction of apoptosis in pancreatic cancer cells: Z. Wang, et al.; Cancer 106, 2503 (2006) Abstract
Curcumin improves wound healing by modulating collagen and decreasing reactive oxygen species: M. Panchatcharam, et al.; Mol. Cell Biochem. 290, 87 (2006) Abstract
Inhibitory effect of curcumin on nitric oxide production from lipopolysaccharide-activated primary microglia: K.K. Jung, et al.; Life Sci. 79, 2022 (2006) Abstract
The dietary compound curcumin inhibits p300 histone acetyltransferase activity and prevents heart failure in rats: T. Morimoto, et al.; J. Clin. Invest. 118, 868 (2008) Abstract; Full Text
 
 
ALX-400-003 Revised 25-May-07
Diethyldithiocarbamic acid . sodium salt . trihydrate (high purity)
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SYNONYMS DETC . 3H2O (high purity)
Diethyldithiocarbamate . 3H2O (high purity)
PRODUCT LINE Oxidative Stress
PRODUCT CATEGORY Spin Traps, Spin Probes & Spin Labels
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ALX-400-003-G005   5 g 17.00 USD Add To Cart
ALX-400-003-G025   25 g 27.00 USD Add To Cart
Product Specification
FORMULA: C5H10NS2 . Na . 3H2O
MW: 148.3 . 23.0 . 54.0
CAS NUMBER: 20624-25-3
MERCK INDEX: 14: 3378
RTECS: EZ6550000
PURITY: ≥99%
APPEARANCE: White to off-white solid.
SOLUBILITY: Soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HAZARD: HARMFUL.

Product Description
Nitric oxide (NO) spin-trapping reagent. Thiol and iron chelator. Inhibits induction of macrophage nitric oxide synthase (NOS). Has been shown to be an inhibitor of the nuclear transcription factor κB (NF-κB).
Product Specific Literature References
On-line detection of nitric oxide formation in liquid aqueous phase by electron paramagnetic resonance spectroscopy: P. Mordvintcev, et al.; Anal. Biochem. 199, 142 (1991) Abstract
NO accounts completely for the oxygenated nitrogen species generated by enzymic L-arginine oxygenation: A. Mülsch, et al.; Biochem. J. 288, 597 (1992) Abstract
Iron diethyldithiocarbamate as spin trap for nitric oxide detection: A.F. Vanin; Methods Enzymol. 301, 269 (1999), Review Abstract
Diethyldithiocarbamate inhibits induction of macrophage NO synthase: A. Mülsch, et al.; FEBS Lett. 321, 215 (1993) Abstract
The relationship between L-arginine-dependent nitric oxide synthesis, nitrite release and dinitrosyl-iron complex formation by activated macrophages: A. Vanin, et al.; Biochim. Biophys. Acta 1177, 37 (1993) Abstract
Dithiocarbamates as potent inhibitors of nuclear factor kappa B activation in intact cells: R. Schreck, et al.; J. Exp. Med. 175, 1181 (1992) Abstract
In vivo spin trapping of nitric oxide generated in the small intestine, liver, and kidney during the development of endotoxemia: a time-course study: G. Wallis, et al.; Shock 6, 274 (1996) Abstract
Epr evidence of nitric oxide production by the regenerating rat liver: M. Obolenskaya, et al.; BBRC 202, 571 (1994) Abstract
EPR and laser flash photolysis studies of the reaction of nitric oxide with water soluble NO trap Fe(II)-proline-dithiocarbamate complex: S.V. Paschenko, et al.; BBRC 225, 577 (1996) Abstract
Complexes of Fe2+ with diethyldithiocarbamate or N-methyl-D-glucamine dithiocarbamate as traps of nitric oxide in animal tissues: comparative investigations: V.D. Mikoyan, et al.; Biochim. Biophys. Acta 1336, 225 (1997) Abstract
NO-dependent mechanisms of adaptation to hypoxia: I.Y. Malyshev, et al.; Nitric Oxide 3, 105 (1999) Abstract
Ex vivo EPR detection of nitric oxide in brain tissue: H. Fujii & L.J. Berliner; Magn. Reson. Med. 42, 599 (1999) Abstract
EPR spectroscopy of common nitric oxide - spin trap complexes: S. Nedeianu & T. Pali; Cell Mol. Biol. Lett. 7, 142 (2002) Abstract
EPR quantification of vascular nitric oxide production in genetically modified mouse models: J.P. Khoo, et al.; Nitric Oxide 10, 156 (2004) Abstract
Evaluation of lipid-based carrier systems and inclusion complexes of diethyldithiocarbamate-iron to trap nitric oxide in biological systems: N. Charlier, et al.; Magn. Reson. Med. 55, 215 (2006) Abstract
Further Categories Containing This Product:
NF-kB Pathway InhibitorsNOS Inhibitors (NOS Induction & Enzyme Activity)
 
 
ALX-280-017 Revised 31-Oct-06
Diethylmaleate
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