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ALX-167-024
Revised 17-May-06
SN50
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Pathway Inhibitors
Ordering Information
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ALX-167-024-C500
500 µg
125.00 USD
Product Specification
SEQUENCE:
H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Leu-Leu-Ala-Pro-Val-Gln-Arg-Lys-Arg-Gln-Lys-Leu-Met-Pro-OH
FORMULA:
C
129
H
230
N
36
O
29
S
MW:
2781.5
PEPTIDE CONTENT:
50-75%
PURITY:
≥96% (HPLC)
APPEARANCE:
White to off-white solid.
FORMULATION:
Counter Ion:
Trifluoroacetate salt.
SOLUBILITY:
Soluble in distilled water, 100% ethanol or ethanol:water 1:1.
RECONSTITUTION:
Reconstitute in sterile distilled water.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Peptides in solution have limited stability.
HANDLING:
Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -20°C.
Product Description
Inhibits translocation of the NF-κB active complex into the nucleus. In mouse endothelial LE-II cells induced with LPS, inhibition of NF-κB nuclear translocation was 88% at 50µg/ml (18µM). In human monocytic THP-1 cells induced by LPS or TNF-α there was 85% inhibition at 100µg/ml.
For control peptide, see SN50M (Prod. No.
ALX-167-025
).
Product Specific Literature References
Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence:
Y.Z. Lin, et al.; J. Biol. Chem.
270
, 14255 (1995)
Abstract
;
Full Text
p38 MAP kinase and MKK-1 co-operate in the generation of GM-CSF from LPS-stimulated human monocytes by an NF-kappa B-independent mechanism:
K.K. Meja, et al.; Br. J. Pharmacol.
131
, 1143 (2000)
Abstract
Nuclear factor-kappaB activates dual inhibition sites in the regulation of tumor necrosis factor-alpha-induced neutrophil apoptosis:
M. Niwa, et al.; Eur. J. Pharmacol
407
, 211 (2000)
Abstract
Activation of nuclear factor kappaB and induction of apoptosis by tumor necrosis factor-alpha in the mouse uterine epithelial WEG-1 cell line:
S. Pampfer, et al.; Biol. Reprod.
63
, 879 (2000)
Abstract
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells
:
Y. Dai, et al.; Blood
103
, 2761 (2004)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Nuclear localization sequence (NLS) aa 360-369 (AAVALLPAVLLALLAPVQRKRQKLMP) of the transcription factor NF-κB p50 linked to a peptide cell-permeablization sequence, the hydrophobic region (h-region) of the signal peptide of Kaposi fibroblast growth factor (K-FGF).
Further Categories Containing This Product:
Peptides
•
Nuclear Envelope & Nuclear Transport
ALX-167-025
Revised 17-May-06
SN50M Control Peptide
PRODUCT LINE
Signal Transduction
PRODUCT CATEGORY
NF-kB Pathway Inhibitors
Ordering Information
Product Numbers:
Format:
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ALX-167-025-C500
500 µg
125.00 USD
Product Specification
SEQUENCE:
H-Ala-Ala-Val-Ala-Leu-Leu-Pro-Ala-Val-Leu-Leu-Ala-Pro-Val-Gln-Arg-Asn-Gly-Gln-Lys-Leu-Met-Pro-OH
FORMULA:
C
123
H
215
N
33
O
30
S
MW:
2668.4
PEPTIDE CONTENT:
70-90%
PURITY:
≥97% (HPLC)
APPEARANCE:
White to off-white solid.
FORMULATION:
Counter Ion:
Trifluoroacetate salt.
SOLUBILITY:
Soluble in water.
RECONSTITUTION:
Reconstitute in sterile distilled water.
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Peptides in solution have limited stability.
HANDLING:
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Product Description
Inactive control peptide for SN50 (Prod. No.
ALX-167-024
). In mouse endothelial LE-II cells, induced with LPS, 50µg/ml SN50M had no measurable effect on NF-κB translocation. In human monocytic THP-1 cells induced by LPS or TNF-α there was slight inhibition at 100µg/ml.
Product Specific Literature References
Inhibition of nuclear translocation of transcription factor NF-kappa B by a synthetic peptide containing a cell membrane-permeable motif and nuclear localization sequence
:
Y.Z. Lin, et al.; J. Biol. Chem.
270
, 14255 (1995)
Abstract
;
Full Text
Interruption of the NF-kappaB pathway by Bay 11-7082 promotes UCN-01-mediated mitochondrial dysfunction and apoptosis in human multiple myeloma cells
:
Y. Dai, et al.; Blood
103
, 2761 (2004)
Abstract
Further Categories Containing This Product:
Peptides
•
Nuclear Envelope & Nuclear Transport
ALX-430-116
Revised 04-Mar-05
Sodium salicylate
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Non-Steroidal Anti-Inflammatory Drugs [NSAIDS]
Ordering Information
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ALX-430-116-G005
5 g
15.00 USD
Product Specification
FORMULA:
C
7
H
5
O
3
Na
MW:
160.1
CAS NUMBER:
54-21-7
MERCK INDEX:
14:
8332
PURITY:
≥99%
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
Product Specific Literature References
Inhibition of NF-kappa B by sodium salicylate and aspirin:
E. Kopp & S. Ghosh; Science
265
, 956 (1994)
Abstract
Neuroprotection by aspirin and sodium salicylate through blockade of NF- kappaB activation:
M. Grilli, et al.; Science
274
, 1383 (1996)
Abstract
The anti-inflammatory agents aspirin and salicylate inhibit the activity of I(kappa)B kinase-beta:
M.J. Yin, et al.; Nature
396
, 77 (1998)
Abstract
The pleiotropic functions of aspirin: mechanisms of action:
A.R. Amin, et al.; Cell Mol. Life Sci.
56
, 305 (1999)
Abstract
Further Categories Containing This Product:
NF-kB Pathway Inhibitors
ALX-201-106
Revised 08-Mar-07
Sp1 Protein (human) (recombinant) (HA)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Sp Protein Family / Related Products
Ordering Information
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Format:
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ALX-201-106-R050
50 µl
377.00 USD
Product Specification
SOURCE/HOST:
Produced in insect cells (Schneider SL2 cells). Human Sp1 is fused to a HA- and a FLAG
®
-tag (H. Braun and G. Suske; Biotechniques
26
, 1038 (1999)).
QUANTITY:
Sufficient for performing at least 25 protein-DNA interaction experiments in 10mM HEPES, pH 7.9, 0.1mM EDTA, 8.5% glycerol, 170mM KCl, 1µM ZnSO
4
, 37.5µg/ml Poly dIdC, 10mM DTT and 1mg/ml BSA, analyzed by gel shift assay.
PURITY DETAIL:
DNA-affinity purified.
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.9, containing 1M KCl, 0.1% NP-40, 12.5mM MgCl
2
and 20% glycerol.
SPECIFIC ACTIVITY:
0.5BFU/µl (band forming units). 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
APPLICATION:
EMSA promoter characterization,
in vitro
transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Description
The ubiquitously expressed Sp1 was the first identified and cloned member of the Sp-family of mammalian transcription factors. The 778 aa human protein contains an 81 aa DNA-binding domain consisting of three C
2
H
2
-type zinc fingers close to the C-terminus. Two glutamine-rich activation domains are present adjacent to serine/threonine stretches in the N-terminal part of the protein. Sp1 recognizes specifically G-rich elements such as the GC-box (GGGGCGGGG) and the GT/CACC-box (GGTGTGGGG) found in housekeeping as well as in many tissue-specific and viral genes. The protein may be employed in the following fields of research:
i) Promoter studies: Identification of Sp1 binding promoter elements by bandshift or DNase1
footprint assays.
ii) Promoter studies: Activation by Sp1 in
in vitro
transcription assays.
iii) Detection of Sp1 interacting proteins.
Product Specific Literature References
Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain:
J.T. Kadanoga, et al.; Cell
51
, 1079 (1987)
Abstract
Vectors for inducible expression of dual epitope-tagged proteins in insect cells:
H. Braun & G. Suske; Biotechniques
26
, 1038 (1999)
Abstract
The Sp-family of transcription factors:
G. Suske; Gene
238
, 291 (1999), (Review)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Nuclear extract was prepared from HA/FLAG-Sp1 expressing SL2 cells and precleared by unspecific DNA-affinity precipitation (multimerized oligonucleotide with HNF-3 binding site). Sp1 was then precipitated with a specific DNA-affinity matrix (multimerized double-stranded GC-box oligonucleotide with the sequence 5´-AGCTTCCGTTGGGGCGGGGCTTCACGTCGA-3´). Bound protein was eluted with 50mM TRIS-HCl, pH 7.9, containing 1M KCl, 0.1% NP40, 20% glycerol, 12.5mM MgCl
2
.
In EMSAs, DNA-protein complexes are separated from free (unbound) oligonucleotides by native gel-electrophoresis. The formation of a specific DNA-protein complex results in a lower mobility of this complex compared to the mobility of the free DNA.
The specificity of the DNA-protein interaction can be analyzed by competition or supershift experiments. An excess of unlabeled specific oligonucleotide interferes with the formation of a specific band, whereas unspecific or mutated oligonucleotides have no effect. The presence of a specific antibody leads to the formation of a very slow migrating antibody-protein-DNA complex.
FLAG is a registered trademark of Sigma-Aldrich Co.
General Literature References
For an extended bibliography
click here.
:
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-107
Revised 17-Nov-06
Sp3 Protein (human) (recombinant) (HA)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
Sp Protein Family / Related Products
Ordering Information
Product Numbers:
Format:
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ALX-201-107-R050
50 µl
377.00 USD
Product Specification
SOURCE/HOST:
Produced in insect cells (Schneider SL2 cells). Human Sp3 is fused to a HA- and a FLAG
®
-tag (H. Braun and G. Suske; Biotechniques
26
, 1038 (1999)).
QUANTITY:
Sufficient for performing at least 25 protein-DNA interaction experiments in 10 mM HEPES (pH 7.9), 0.1 mM EDTA, 8.5 % Glycerol, 170 mM KCl, 1 µM ZnSO
4
, 37.5 µg/ml Poly dIdC, 10 mM DTT, and 1 mg/ml BSA, analyzed by gel shift assay.
PURITY:
~50% (SDS-PAGE)
FORMULATION:
Liquid. In 20mM TRIS-Cl, pH 7.9, containing 150mM KCl, 0.2mM EDTA and 20% glycerol.
SPECIFICITY:
Binds specifically to an oligonucleotide with the sequence: GGG GCG GGG.
SPECIFIC ACTIVITY:
>0.5 BFU/µl (band forming units) 1BFU is sufficient to generate a strong band shift with a labelled oligonucleotide.
APPLICATION:
EMSA promoter characterization,
in vitro
transcription assays, analysis of nuclear extracts (oligonucleotides and recombinant protein serve as a positive control).
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
Product Specific Literature References
The Sp-family of transcription factors:
G. Suske; Gene
238
, 291 (1999), (Review)
Abstract
Vectors for inducible expression of dual epitope-tagged proteins in insect cells:
H. Braun & G. Suske; Biotechniques
26
, 1038 (1999)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
FLAG is a registered trademark of Sigma-Aldrich Co.
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-306-022
Revised 07-Feb-05
D-erythro-Sphingosine chloride, N,N,N-Trimethyl-
SYNONYMS
N,N,N-Trimethyl-D-
erythro
-sphingosine chloride
PRODUCT LINE
Neurobiology
PRODUCT CATEGORY
Ceramides / Cerebrosides / Sphingolipids / Related Products
Ordering Information
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ALX-306-022-M005
5 mg
90.00 USD
Product Specification
FORMULA:
C
21
H
44
ClNO
2
MW:
378.0
SOURCE/HOST:
Synthetic.
APPEARANCE:
White crystalline solid.
SOLUBILITY:
Soluble in water, methanol or DMSO; slightly soluble in chloroform.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
HANDLING:
Keep under inert gas. Hygroscopic.
Product Description
Stable N-methylated derivative of sphingosine that exhibits antitumor and anti-inflammatory properties and inhibits platelet activating factor (PAF) activation and protein kinase C (PKC) activity. Inhibits IL-1β-induced NF-κB activation and cell surface expression of crucial selectins which promote adhesion of Le
x
- or sialosyl-Le
x
-expressing cells with platelets and endothelial cells.
Product Specific Literature References
Downregulation of GMP-140 (CD62 or PADGEM) expression on platelets by N,N-dimethyl and N,N,N-trimethyl derivatives of sphingosine:
K. Handa, et al.; Biochemistry
30
, 11682 (1991)
Abstract
Cell membrane signaling as target in cancer therapy. II: Inhibitory effect of N,N,N-trimethylsphingosine on metastatic potential of murine B16 melanoma cell line:
H. Okoshi, et al.; Cancer Res.
51
, 6019 (1991)
Abstract
Effect of sphingosine and its N-methyl derivatives on oxidative burst, phagokinetic activity, and trans-endothelial migration of human neutrophils:
S. Kimura, et al.; Biochem. Pharmacol.
44
, 1585 (1992)
Abstract
Liposomal N,N,N-trimethylsphingosine (TMS) as an inhibitor of B16 melanoma cell growth and metastasis with reduced toxicity and enhanced drug efficacy compared to free TMS: cell membrane signaling as a target in cancer therapy III:
Y.S. Park, et al.; Cancer Res.
54
, 2213 (1994)
Abstract
N,N,N-trimethylsphingosine inhibits interleukin-1 beta-induced NF-kappa B activation and consequent E-selectin expression in human umbilical vein endothelial cells:
A. Masamune, et al.; FEBS Lett.
367
, 205 (1995)
Abstract
Lysophosphatidylcholine promotes P-selectin expression in platelets and endothelial cells. Possible involvement of protein kinase C activation and its inhibition by nitric oxide donors:
T. Murohara, et al.; Circ. Res.
78
, 780 (1996)
Abstract
;
Full Text
Further Categories Containing This Product:
Antitumor Agents (Enzyme Inhibitors)
•
PKC Inhibitors
•
Anti-inflammatory Agents Other Products
•
NF-kB Pathway Inhibitors
ALX-430-105
Revised 10-Dec-04
Sulindac
SYNONYMS
MK-231
(1Z)-5-Fluoro-2-methyl-1-[[4-(methylsulfinyl)phenyl]methylene]-1
H
-indene-3-acetic acid
cis
-5-Fluoro-2-methyl-1-[
p
-(methylsulfinyl)benzylidene]-indene-3-acetic acid
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Non-Steroidal Anti-Inflammatory Drugs [NSAIDS]
Ordering Information
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ALX-430-105-G001
1 g
10.00 USD
Product Specification
FORMULA:
C
20
H
17
FO
3
S
MW:
356.4
CAS NUMBER:
38194-50-2
MERCK INDEX:
14:
8982
RTECS:
NK8226000
PURITY:
≥98%
APPEARANCE:
Yellow solid.
SOLUBILITY:
Soluble in DMSO or aqueous bases.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
Product Description
Inhibits the NF-κB pathway. Non-steroidal anti-inflammatory drug. Reduces the level and activity of several cyclin-dependent kinases (CDKs) and induces apoptosis. For the active metabolite sulindac sulfide see Prod. No.
ALX-430-106
.
Further Categories Containing This Product:
NF-kB Pathway Inhibitors
•
Apoptosis Inducers & Inhibitors Other Products
•
COX Inhibitors
ALX-430-106
Revised 31-Aug-04
Sulindac sulfide
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Non-Steroidal Anti-Inflammatory Drugs [NSAIDS]
Ordering Information
Product Numbers:
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ALX-430-106-M005
5 mg
32.00 USD
ALX-430-106-M025
25 mg
85.00 USD
Product Specification
FORMULA:
C
20
H
17
FO
2
S
MW:
340.4
CAS NUMBER:
32004-67-4
PURITY:
≥98%
APPEARANCE:
Yellow-orange solid.
SOLUBILITY:
Soluble in 100% ethanol or DMSO.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
Product Description
Active metabolite of sulindac (Prod. Nr.
ALX-430-105
). NF-κB inhibitor. Induces apoptosis. Inhibits Ras signaling. Also inhibits cyclooxygenase (COX).
Product Specific Literature References
Antineoplastic drugs sulindac sulfide and sulfone inhibit cell growth by inducing apoptosis
:
G.A. Piazza, et al.; Cancer Res.
55
, 3110 (1995)
Abstract
Sulindac sulfide inhibits Ras signaling
:
C. Herrmann, et al.; Oncogene
17
, 1769 (1998)
Abstract
Sulindac inhibits activation of the NF-kappaB pathway
:
Y. Yamamoto, et al.; J. Biol. Chem.
274
, 27307 (1999)
Abstract
;
Full Text
Further Categories Containing This Product:
NF-kB Pathway Inhibitors
•
COX Inhibitors
•
Apoptosis Inducers & Inhibitors Other Products
ALX-430-107
Revised 20-Aug-08
Sulindac sulfone
PRODUCT LINE
Inflammation
PRODUCT CATEGORY
Non-Steroidal Anti-Inflammatory Drugs [NSAIDS]
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
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ALX-430-107-M005
5 mg
30.00 USD
ALX-430-107-M050
50 mg
90.00 USD
Product Specification
FORMULA:
C
20
H
17
FO
4
S
MW:
372.4
CAS NUMBER:
59864-04-9
PURITY:
≥98% (HPLC)
APPEARANCE:
Yellow solid.
SOLUBILITY:
Soluble in DMSO or 100% ethanol (warm).
SHIPPING:
AMBIENT
LONG TERM STORAGE:
+20°C
Product Description
Metabolite of sulindac (Prod. No.
ALX-430-105
).
Product Specific Literature References
Sulindac inhibits activation of the NF-kappaB pathway:
Y. Yamamoto, et al.; J. Biol. Chem.
274
, 27307 (1999)
Abstract
;
Full Text
Further Categories Containing This Product:
COX Inhibitors
•
NF-kB Pathway Inhibitors
ALX-350-261
Revised 07-Oct-08
Trichodion
PRODUCT LINE
Natural Products / Antibiotics
PRODUCT CATEGORY
Other Natural Products - DNA Regulation / Transcription
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-350-261-C100
100 µg
85.00 USD
ALX-350-261-C500
500 µg
250.00 USD
Product Specification
FORMULA:
C
12
H
14
O
5
MW:
238.2
SOURCE/HOST:
Isolated from
Trichosporiella sp
.
PURITY:
≥95% (HPLC)
APPEARANCE:
White to off-white powder.
SOLUBILITY:
Soluble in DMSO, 100% ethanol or methanol.
SHIPPING:
AMBIENT
LONG TERM STORAGE:
-20°C
Product Description
Cytotoxic. Inhibits NF-κB (IC
50
=42-84µM), AP-1 (IC
50
=21µM) and STAT1α mediated gene expression induced by IFN-γ resulting in inhibition of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS; NOS ll) expression.
Product Specific Literature References
Trichodion, a new inhibitor of inflammatory signal transduction pathways from a Tri
