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Monoclonal Antibodies
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ALX-804-528 Revised 10-Oct-08
Monoclonal Antibody to BimS/EL/L (10B12)
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SYNONYMS anti-BCL2L11 MAb (10B12)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bim / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-528-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE: 10B12
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.
SPECIFICITY: Recognizes an epitope within aa 20-40 of human, mouse and monkey BimL, BimEL, and BimS. Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region recently identified.
APPLICATION: ELISA
Flow Cytometry
Immunocytochemistry
Immunohistochemistry (only EM)
Immunoprecipitation (2µg/ml)
Western Blot (1-5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008) Abstract; Full Text
General Literature References
A rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998) Abstract
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science 286, 1735 (1999) Abstract
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell 3, 287 (1999) Abstract
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O'Reilly, et al.; Am. J. Pathol. 157, 449 (2000) Abstract; Full Text
Molecular cloning and characterization of six novel isoforms of human Bim, a member of the proapoptotic Bcl-2 family: U et al.; FEBS Lett. 509, 135 (2001) Abstract
Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis: Marani et al.; Mol. Cell. Biol. 22, 3577 (2002) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-351 Revised 18-Sep-07
Monoclonal Antibody to BimS/EL/L (14A8)
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SYNONYMS anti-BCL2L11 MAb (14A8)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bim / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-351-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE: 14A8
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 10mM sodium azide.
IMMUNOGEN: Recombinant mouse BimS.
SPECIFICITY: Recognizes human, mouse and monkey BimL, BimEL and BimS.
APPLICATION: Flow Cytometry (0.5-1µg/ml; overexpressing cell lines)
Immunocytochemistry (1-5µg/ml)
Immunohistochemistry (paraffin sections (1-5µg/ml))
Immunoprecipitation (1-2µg/ml)
Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998) Abstract
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science 286, 1735 (1999) Abstract
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell 3, 287 (1999) Abstract
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O'Reilly, et al.; Am. J. Pathol. 157, 449 (2000) Abstract; Full Text
Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis: G.V. Putcha, et al.; Neuron 29, 615 (2001) Abstract
BH3-only Bcl-2 family member Bim is required for apoptosis of autoreactive thymocytes: P. Bouillet, et al.; Nature 415, 922 (2002) Abstract
Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells: Y. Zhu, et al.; PNAS 101, 7681 (2004) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION As Bim expression is generally low in non-transfected cell lines, please take care when choosing positive controls. Examples of cell lines which express detectable levels of Bim include: B6.2.16.BW2 or KO52.DA20 (T cell lymphomas) or ABL5.8.1 (B cell lymphoma) or MCF-7s. It is generally necessary to use a high amount of lysate (e.g. 1 x 10^7 cells). When using tissue samples, it is generally necessary to use 1-5mg of total protein from an appropriate tissue source.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-527 Revised 10-Oct-08
Monoclonal Antibody to BimS/EL/L (3C5)
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SYNONYMS anti-BCL2L11 MAb (3C5)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bim / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-527-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Monkey
CLONE: 3C5
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml.
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 20-40 (Q20PAERPPQLRPGAPTSLQTEP40) of mouse BimL.
SPECIFICITY: Recognizes an epitope within aa 20-40 of human, mouse and monkey BimL, BimEL, and BimS. Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region recently identified.
APPLICATION: ELISA
Flow Cytometry
Immunocytochemistry
Immunoprecipitation (2µg/ml)
Western Blot (1-5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Bim and Bad mediate imatinib-induced killing of Bcr/Abl+ leukemic cells, and resistance due to their loss is overcome by a BH3 mimetic: J. Kuroda, et al.; PNAS 103, 14907 (2006) Abstract; Full Text
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008) Abstract; Full Text
General Literature References
A rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998) Abstract
Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity: P. Bouillet, et al.; Science 286, 1735 (1999) Abstract
The proapoptotic activity of the Bcl-2 family member Bim is regulated by interaction with the dynein motor complex: H. Puthalakath, et al.; Mol. Cell 3, 287 (1999) Abstract
The proapoptotic BH3-only protein bim is expressed in hematopoietic, epithelial, neuronal, and germ cells: L.A. O'Reilly, et al.; Am. J. Pathol. 157, 449 (2000) Abstract; Full Text
Molecular cloning and characterization of six novel isoforms of human Bim, a member of the proapoptotic Bcl-2 family: U et al.; FEBS Lett. 509, 135 (2001) Abstract
Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis: Marani et al.; Mol. Cell. Biol. 22, 3577 (2002) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-508 Revised 10-Oct-08
Monoclonal Antibody to Bmf (mouse/rat) (17A9)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bmf / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-508-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
CLONE: 17A9
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 (D13VFQSEDGEPGTQPG27) and aa 112-126 (A112GSPLGEQPPEGQFL126) of mouse Bmf.
SPECIFICITY: Recognizes mouse and rat Bmf. Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL. Does not cross-react with human Bmf.
APPLICATION: ELISA
Flow Cytometry
Immunoprecipitation
Western Blot
Other applications not tested.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Broad degradation of proapoptotic proteins with the conserved Bcl-2 homology domain 3 during infection with Chlamydia trachomatis: S. Ying, et al.; Infect. Immun. 73, 1399 (2005) Abstract; Full Text
Loss of the BH3-only protein Bmf impairs B cell homeostasis and accelerates gamma irradiation-induced thymic lymphoma development: V. Labi, et al.; J. Exp. Med. 205, 641 (2008) Abstract; Full Text
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Methodology Abstract
Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science 293, 1829 (2001), Methodology Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-529 Revised 09-Nov-04
Monoclonal Antibody to Bmf (mouse/rat) (17C2)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bmf / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-529-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Mouse
Rat
CLONE: 17C2
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml.
FORMULATION: Liquid. Protein G affinity purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant full length mouse Bmf and synthetic peptides corresponding to aa 13-27 (D13VFQSEDGEPGTQPG27) and aa 112-126 (A112GSPLGEQPPEGQFL126) of mouse Bmf.
SPECIFICITY: Recognizes mouse and rat Bmf. Detects bands of ~25kDa and ~30kDa by Western blot, corresponding to endogenous Bmf and BmfL. Does not cross-react with human Bmf.
APPLICATION: ELISA
Flow Cytometry
Western Blot
Other applications not tested.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Literature References
Rapid hybridoma screening method for the identification of monoclonal antibodies to low-abundance cytoplasmic proteins: L.A. O'Reilly, et al.; Biotechniques 25, 824 (1998), Methodology Abstract
Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science 293, 1829 (2001), Methodology Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-343 Revised 09-Mar-05
Monoclonal Antibody to Bmf (9G10)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bmf / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-343-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 9G10
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Protein G-affinity purified antibody in PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse Bmf.
SPECIFICITY: Recognizes human and mouse Bmf.
APPLICATION: Flow Cytometry
Immunoprecipitation
Western Blot (excellent)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science 293, 1829 (2001) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-342 Revised 18-Jan-08
Monoclonal Antibody to Bmf (12E10)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Bmf / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-342-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: 12E10
ISOTYPE: Rat IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant mouse Bmf.
SPECIFICITY: Recognizes human and mouse Bmf.
APPLICATION: Flow Cytometry (excellent)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis: H. Puthalakath, et al.; Science 293, 1829 (2001) Abstract
Bim and Bad mediate imatinib-induced killing of Bcr/Abl+ leukemic cells, and resistance due to their loss is overcome by a BH3 mimetic: J. Kuroda, et al.; PNAS 103, 14907 (2006) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-647 Revised 13-Oct-06
Monoclonal Antibody to Bradykinin (MBK3)
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PRODUCT LINE Neurobiology
PRODUCT CATEGORY Bradykinin Receptor / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-647-R100   100 µl 210.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: MBK3
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Undiluted ascites.
IMMUNOGEN: Bradykinin (H-Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg-OH).
SPECIFICITY: Recognizes bradykinin from various species.
APPLICATION: Western Blot (1:1’000-1:4’000)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Anti-idiotypic antibodies bearing the internal image of a bradykinin epitope. Production, characterization, and interaction with the kinin receptor: M. Haasemann, et al.; J. Immunol. 147, 3882 (1991) Abstract
Related Products
Further Categories Containing This Product:
NOS Regulation • Cell Stress, Oxidative Stress & Redox Signalling Other Products • Monoclonal Antibodies
 
 
ALX-801-036 Revised 15-Oct-07
Monoclonal Antibody to Breast Cancer Resistance Protein (BXP-53)
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SYNONYMS anti-BCRP MAb (BXP-53)
anti-MXR MAb (BXP-53)
anti-ABCG2 MAb (BXP-53)
anti-CD338 MAb (BXP-53)
PRODUCT LINE Cancer
PRODUCT CATEGORY Breast Cancer Resistance Protein [BCRP / MXR] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-036-C125   125 µg 350.00 USD Add To Cart
ALX-801-036-C250   250 µg 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: BXP-53
ISOTYPE: Rat IgG2a
CONCENTRATION: ~250µg/ml
FORMULATION: Liquid. In serum free cell culture supernatant containing 0.7% BSA and 0.1% sodium azide.
IMMUNOGEN: Recombinant mouse BCRP (breast cancer resistance protein; MXR) (aa 221-394).
SPECIFICITY: Recognizes an internal epitope of human and mouse BCRP.
APPLICATION: Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations.
Immunohistochemistry (frozen sections, paraffin sections): Use 1:20 on acetone fixed frozen sections followed by incubation with biotin-labelled rabbit anti-rat IgG and HRP-labelled streptavidin.
For paraffin sections see [1].
Western Blot: Use 1:20-1:50 dilution and  HRP-labelled anti-rat IgG.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
Product Specific Literature References
[1] The breast cancer resistance protein protects against a major chlorophyll-derived dietary phototoxin and protoporphyria: J.W. Jonker, et al.; PNAS 99, 15649 (2002) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies • Tumor Markers • CD Antibodies
 
 
ALX-801-029 Revised 15-Oct-07
Monoclonal Antibody to Breast Cancer Resistance Protein (human) (BXP-21)
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SYNONYMS anti-BCRP (human) MAb (BXP-21)
anti-MXR (human) MAb (BXP-21)
anti-ABCG2 (human) MAb (BXP-21)
anti-CD338 (human) MAb (BXP-21)
PRODUCT LINE Cancer
PRODUCT CATEGORY Breast Cancer Resistance Protein [BCRP / MXR] / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-029-C125   125 µg 350.00 USD Add To Cart
ALX-801-029-C250   250 µg 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
<