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PARP-4 [Minor Vault Protein p193] / Related Products
You are here: Product Lines > DNA Regulation / Transcription > Poly(ADP-ribose) Polymerase [PARP] / Related Products > PARP-4 [Minor Vault Protein p193] / Related Products
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ALX-201-286 Revised 19-May-08
Minor Vault p193 Protein (human) (recombinant) (His)
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SYNONYMS VPARP (human) (recombinant) (His)
PARP-4 (human) (recombinant) (His)
PRODUCT LINE Cancer
PRODUCT CATEGORY Minor Vault Protein p193 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-201-286-C010   10 µg 270.00 USD Add To Cart
Product Specification
EC: 2.4.2.30
SOURCE/HOST: Produced in E. coli. Human minor vault p193 protein is fused at the C-terminus to a His-tag.
CONCENTRATION: 0.5mg/ml
PURITY: ≥98%
FORMULATION: Liquid. In PBS containing 50% glycerol.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
In silico characterization of the family of PARP-like poly(ADP-ribosyl)transferases (pARTs): H. Otto, et al.; BMC Genomics 6, 139 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9UKK3: PARP4 (human)
General Literature References
The 193-kD vault protein, VPARP, is a novel poly(ADP-ribose) polymerase: V.A. Kickhoefer, et al.; J. Cell Biol. 146, 917 (1999) Abstract; Full Text
Further Categories Containing This Product:
PARP Other ProductsRecombinant Proteins / Fusion Proteins
 
 
ALX-801-025 Revised 25-Oct-06
Monoclonal Antibody to Minor Vault p193 Protein (human) (p193-10)
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SYNONYMS anti-VPARP (human) MAb (p193-10)
anti-PARP-4 (human) MAb (p193-10)
PRODUCT LINE Cancer
PRODUCT CATEGORY Minor Vault Protein p193 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-025-C125   125 µg 350.00 USD Add To Cart
ALX-801-025-C250   250 µg 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: p193-10
ISOTYPE: Mouse IgG2a
CONCENTRATION: ~250µg/ml
FORMULATION: Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
IMMUNOGEN: Minor vault p193 protein (VPARP; PARP-4).
SPECIFICITY: Recognizes epitope aa 506-510 (VALGK) of human minor vault p193 protein.
APPLICATION: Immunohistochemistry: Use 1:10-1:20 dilution for staining of paraformaldehyde (4%) fixed cytospin preparations and frozen tissue sections. Apply the following pretreatment: 10 min in 20mM glycine in PBS (pH 7.5) and 10 min in 6N guanidine hydrochloride in 50mM TRIS-HCl (pH 7.5); Schroeijers et al., 2000.
Western Blot: Use 1:10-1:20 dilution (ECL)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines: A.B. Schroeijers, et al.; Cancer Res. 60, 1104 (2000) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-801-023 Revised 25-Oct-06
Monoclonal Antibody to Minor Vault p193 Protein (human) (p193-4)
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SYNONYMS anti-VPARP (human) MAb (p193-4)
anti-PARP-4 (human) MAb (p193-4)
PRODUCT LINE Cancer
PRODUCT CATEGORY Minor Vault Protein p193 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-023-C125   125 µg 350.00 USD Add To Cart
ALX-801-023-C250   250 µg 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: p193-4
ISOTYPE: Mouse IgG1
CONCENTRATION: ~250µg/ml
FORMULATION: Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide.
IMMUNOGEN: Minor vault p193 protein (VPARP; PARP-4).
SPECIFICITY: Recognizes epitope aa 491-494 of human minor vault p193 protein.
APPLICATION: Immunocytochemistry (cytospin preparations)
Immunohistochemistry (frozen sections, paraffin sections)
Immunoprecipitation
Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines: A.B. Schroeijers, et al.; Cancer Res. 60, 1104 (2000) Abstract; Full Text
Multiple human vault RNAs. Expression and association with the vault complex: A. van Zon, et al.; J. Biol. Chem. 276, 37715 (2001) Abstract; Full Text
Up-regulation of drug resistance-related vaults during dendritic cell development: A.B. Schroeijers, et al.; J. Immunol. 168, 1572 (2002) Abstract
The formation of vault-tubes: a dynamic interaction between vaults and vault PARP: A. Van Zon, et al.; J. Cell Sci. 116, 4391 (2003) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-801-024 Revised 25-Oct-06
Monoclonal Antibody to Minor Vault p193 Protein (human) (p193-6)
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SYNONYMS anti-VPARP (human) MAb (p193-6)
anti-PARP-4 (human) MAb (p193-6)
PRODUCT LINE Cancer
PRODUCT CATEGORY Minor Vault Protein p193 / Related Products
Ordering Information
Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-801-024-C125   125 µg 350.00 USD Add To Cart
ALX-801-024-C250   250 µg 630.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: p193-6
ISOTYPE: Mouse IgG2b
CONCENTRATION: ~250µg/ml.
FORMULATION: Liquid. In serum free cell culture supernatant containing 1% BSA and 0.1% sodium azide.
IMMUNOGEN: Minor vault p193 protein (VPARP; PARP-4).
SPECIFICITY: Recognizes epitope aa 593-599 (FSKVEDY) of human minor vault p193 protein.
APPLICATION: Immunocytochemistry (4% paraformaldehyde cytospin preparations (1:10-1:20))
Immunohistochemistry (frozen sections (1:10-1:20))
Apply the following treatment: 10 minutes in 20 mM glycine in PBS, PH 7.5 and 110 minutes 6N guanidine hydrochloride in 50mM TRIS-HCl, pH 7.5 [1].
Western Blot (ECL detection)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
Product Specific Literature References
[1] The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines: A.B. Schroeijers, et al.; Cancer Res. 60, 1104 (2000) Abstract; Full Text
Further Categories Containing This Product:
Monoclonal Antibodies
 
 

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