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Items 14 of 14
ALX-201-277
Revised 24-Sep-07
Apurinic/Apyrimidinic Endonuclease (human) (recombinant)
SYNONYMS
APE1 (human) (recombinant)
APEX (human) (recombinant)
HAP1 (human) (recombinant)
Ref-1 (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-277-C010
10 µg
260.00 USD
Product Specification
MW:
~36kDa
SOURCE/HOST:
Produced in
E. coli
.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE).
FORMULATION:
Liquid. In 20mM HEPES-KOH, pH 7.5, containing 100mM NaCl and 20% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Enzyme is freshly produced upon customer request and is stable
for ~2 weeks at -20°C.
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
The checkpoint clamp, Rad9-Rad1-Hus1 complex, preferentially stimulates the activity of apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta in long patch base excision repair:
A. Gembka, et al.; Nucleic Acids Res.
35
, 2596 (2007)
Abstract
;
Full Text
Related Products
ALX-201-278
DNA Ligase I (human) (recombinant) (His)
ALX-201-276
DNA Polymerase λ (human) (recombinant)
ALX-201-295
DNA Polymerase β (human) (recombinant)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-201-077
Revised 10-Jul-07
DNase I-like Endonuclease 3 (human) (recombinant)
SYNONYMS
DNAS1L3 (human) (recombinant)
Deoxyribonuclease γ (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-077-C010
10 µg
350.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 25mM TRIS-HCl, pH 7.5, containing 150mM potassium chloride and 50% glycerol.
APPLICATION:
For studying DNAS1L3 catalyzed oligonucleosomal DNA fragmentation.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
Product Specific Literature References
Role of DNAS1L3 in Ca2+- and Mg2+-dependent cleavage of DNA into oligonucleosomal and high molecular mass fragments:
A.G. Yakovlev, et al.; Nucl. Acids Res.
27
, 1999 (1999)
Abstract
A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by Poly(ADP-ribose) polymerase:
A.G. Yakovlev, et al.; J. Biol. Chem.
275
, 21302 (2000)
Abstract
;
Full Text
Regulation of DNAS1L3 endonuclease activity by poly(ADP-ribosyl)ation during etoposide-induced apoptosis. Role of poly(ADP-ribose) polymerase-1 cleavage in endonuclease activation
:
A.H. Boulares, et al.; J. Biol. Chem.
277
, 372 (2002)
Abstract
;
Full Text
The Poly(ADP-ribose) polymerase-1-regulated endonuclease DNAS1L3 is required for etoposide-induced internucleosomal DNA fragmentation and increases etoposide cytotoxicity in transfected osteosarcoma cells
:
A.H. Boulares, et al.; Cancer Res.
62
, 4439 (2002)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
For maximum activity, use at pH 7.5 with 2.5mM Ca
2+
and 5mM Mg
2+
. Causes single strand nicks in duplex DNA and can be inhibited by Zn
2+
, but not by actin.
ALX-201-244
Revised 27-Mar-07
Endonuclease G (human) (recombinant)
SYNONYMS
EndoG (human) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
HtrA2 / Omi, ARTS, EndoG / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-244-C020
20 µg
190.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Recombinant human endonuclease G is fused to a His-tag at the C-terminus.
CONCENTRATION:
0.4mg/ml
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 8.0, containing 500mM sodium chloride, 0.05% Tween 20, 5mM β-mercaptoethanol, 500mM imidazole and 20% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Images
Please click on thumbnails to enlarge.
Further Categories Containing This Product:
DNA Endonucleases / Related Products
•
Recombinant Proteins / Fusion Proteins
ALX-201-230
Revised 26-Apr-06
Endonuclease G (rat) (recombinant)
SYNONYMS
EndoG (rat) (recombinant)
PRODUCT LINE
Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY
HtrA2 / Omi, ARTS, EndoG / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-230-C020
20 µg
190.00 USD
Product Specification
CONCENTRATION:
0.4mg/ml
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 8.0, containing 500mM sodium chloride, 0.05% Tween 20, 5mM β-mercaptoethanol, 500mM imidazole and 20% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Further Categories Containing This Product:
DNA Endonucleases / Related Products
•
Recombinant Proteins / Fusion Proteins
ALX-201-249
Revised 18-Mar-08
FEN-1 (human) (recombinant)
SYNONYMS
Flap Structure-specific Endonuclease 1 (human) (recombinant)
MF1 (human) (recombinant)
Maturation Factor 1 (human) (recombinant)
DNase IV (human) (recombinant)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-249-C010
10 µg
260.00 USD
Product Specification
MW:
~42kDa
SOURCE/HOST:
Produced in
E. coli
.
CONCENTRATION:
1mg/ml
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 1mM DTT, 1mM EDTA and 0.02% Nonidet P40.
SHIPPING:
SHIPPED ON DRY ICE
SHORT TERM STORAGE:
-20°C
LONG TERM STORAGE:
-80°C
USE/STABILITY:
Stable for at least 4 weeks when stored at -20°C.
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Flap endonuclease 1: a novel tumour suppresser protein:
G. Henneke, et al.; TIBS
28
, 384 (2003)
Abstract
The Fen1 extrahelical 3’-flap pocket is conserved from archaea to human and regulates DNA substrate specificity:
E. Friedrich-Heineken and U. Hubscher; Nucl. Acids Res.
32
, 2520 (2004)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P39748:
FEN-1 (human)
Further Categories Containing This Product:
Recombinant Proteins / Fusion Proteins
ALX-804-213
Revised 14-Feb-08
Monoclonal Antibody to Apurinic/Apyrimidinic Endonuclease (human) (13B 8E5C2)
SYNONYMS
anti-APE1 (human) MAb (13B 8E5C2)
anti-APEX (human) MAb (13B 8E5C2)
anti-HAP1 (human) MAb (13B 8E5C2)
anti-Ref-1 (human) MAb (13B 8E5C2)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-804-213-R100
100 µl
379.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
13B 8E5C2
ISOTYPE:
Mouse IgG2a
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:
Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
SPECIFICITY:
Recognizes human APE1. Detects a band of ~37kDa by Western blot.
APPLICATION:
Immunohistochemistry (paraffin sections (1:100))
Immunoprecipitation
Western Blot (1:1’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Elevated and altered expression of the multifunctional DNA base excision repair and redox enzyme Ape1/ref-1 in prostate cancer:
M.R. Kelley, et al.; Clin. Cancer Res.
7
, 824 (2001)
Abstract
Alterations in the expression of the DNA repair/redox enzyme APE/ref-1 in epithelial ovarian cancers:
D.H. Moore, et al.; Clin. Cancer Res.
6
, 602 (2000)
Abstract
General Information
Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κ B, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5’ of an AP site to produce a hydroxyl group at the 3’ terminus of an unmodified nucleotide upstream of the nick and a 5’-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5’-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3’-5’ DNA exonuclease activity, a 3’-phosphodiesterase activity, a 3’-phosphatase activity, and a RNase H activity. Recently it has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3’ termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-011
Revised 19-Nov-08
Monoclonal Antibody to Deoxyribonuclease I (1E3)
SYNONYMS
anti-DNase I MAb (1E3)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
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ALX-804-011-L002
2 ml
320.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Rat
Others
CLONE:
1E3
ISOTYPE:
Mouse IgM
FORMULATION:
Sterile culture supernatant. Contains 0.1% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 1-23 of N-terminal bovine DNase I (deoxyribonuclease I).
SPECIFICITY:
Recognizes the N-terminal domain of human, rat and bovine DNase I.
APPLICATION:
Immunohistochemistry (paraffin sections)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
Product Specific Literature References
Characterization of the endogenous deoxyribonuclease involved in nuclear DNA degradation during apoptosis (programmed cell death):
M.C. Peitsch, et al.; EMBO J.
12
, 371 (1993)
Abstract
Distribution of deoxyribonuclease I in rat tissues and its correlation to cellular turnover and apoptosis (programmed cell death):
B. Polzar, et al.; Eur. J. Cell Biol.
64
, 200 (1994)
Abstract
A new function for an old enzyme: the role of DNase I in apoptosis:
H.G. Mannherz, et al. ; Current Topics in Microbiology and Immunolgy, vol. 198: Pathways for Cytolysis (G.M. Griffith & J. Tschopp, eds.), Springer Verlag, Berlin & Heidelberg 161 (1995)
Localization of deoxyribonuclease I gene transcripts and protein in rat tissues and its correlation with apoptotic cell elimination:
S. Zanotti, et al.; Histochemistry
103
, 369 (1995)
Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
ALX-804-733
Revised 05-Feb-08
Monoclonal Antibody to Ribosomal Protein S3 (human) (RP159-1)
SYNONYMS
anti-RPS3 (human) MAb (RP159-1)
anti-40S Ribosomal Protein S3 (human) MAb (RP159-1)
anti-IMR-90 Ribosomal Protein S3 (human) MAb (RP159-1)
PRODUCT LINE
Protein Synthesis, Modification & Degradation
PRODUCT CATEGORY
Ribosomal Proteins / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-804-733-C100
100 µg
380.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE:
RP159-1
ISOTYPE:
Mouse IgG1
CONCENTRATION:
1mg/ml
FORMULATION:
Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
IMMUNOGEN:
Recombinant human ribosomal protein S3.
SPECIFICITY:
Recognizes human ribosomal protein S3.
APPLICATION:
ELISA (direct and indirect: 1:500-1:5’000)
Western Blot (1:500-1:5’000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application.
SHIPPING:
SHIPPED ON BLUE ICE
SHORT TERM STORAGE:
+4°C
LONG TERM STORAGE:
-20°C
USE/STABILITY:
Stable for 1-2 weeks when stored at +4°C.
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Interaction of Hsp90 with ribosomal proteins protects from ubiquitination and proteasome-dependent degradation:
T.S. Kim, et al.; Mol. Biol. Cell
17
, 824 (2006)
Abstract
;
Full Text
Reduction of invasion in human fibrosarcoma cells by ribosomal protein S3 in conjunction with Nm23-H1 and ERK:
S.H. Kim & J. Kim; Biochim. Biophys. Acta
1763
, 823 (2006)
Abstract
Ribosomal protein S3: a KH domain subunit in NF-kappaB complexes that mediates selective gene regulation:
F. Wan, et al.; Cell
131
, 927 (2007)
Abstract
General Information
MANUFACTURER
Manufactured by AdipoGen, Inc.
Related Products
ALX-201-408
Ribosomal Protein S3 (human) (recombinant) (His)
ALX-201-410
Ribosomal Protein S3 (human) (recombinant)
ALX-210-481
Polyclonal Antibody to Ribosomal Protein S3 (human)
Further Categories Containing This Product:
Co-Chaperones & HSP-interacting Proteins / Related Products
•
DNA Endonucleases / Related Products
•
NF-kB Pathway Other Products
•
DNA Endonucleases / Related Products
•
MAPK Pathway Other Products
•
Monoclonal Antibodies
ALX-210-552
Revised 31-Oct-06
Polyclonal Antibody to Apurinic/Apyrimidinic Endonuclease
SYNONYMS
anti-APE1 PAb
anti-APEX PAb
anti-HAP1 PAb
anti-Ref-1 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-552-R100
100 µl
290.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
FORMULATION:
Purified. Contains 0.05% sodium azide.
IMMUNOGEN:
Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
SPECIFICITY:
Recognizes human, mouse and rat APE1. Detects a band of ~37kDa by Western blot.
APPLICATION:
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Product Description
Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-κB, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5' of an AP site to produce a hydroxyl group at the 3' terminus of an unmodified nucleotide upstream of the nick and a 5'-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5'-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3'-5' DNA exonuclease activity, a 3'-phosphodiesterase activity, a 3'-phosphatase activity, and a RNase H activity. Recently it has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3' termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.
Product Specific Literature References
Apurinic endonuclease (Ref-1) is induced in mammalian cells by oxidative stress and involved in clastogenic adaptation:
S. Grosch, et al.; Cancer Res.
58
, 4410 (1998)
Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-723
Revised 29-Nov-04
Polyclonal Antibody to Apurinic/Apyrimidinic Endonuclease
SYNONYMS
anti-APE1 PAb
anti-APEX PAb
anti-HAP1 PAb
anti-Ref-1 PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-723-R100
100 µl
280.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. 100µg/ml affinity-purified rabbit antiserum in PBS. Contains no preservatives.
IMMUNOGEN:
Recombinant human APE1 (apurinic/apyrimidinic endonuclease).
SPECIFICITY:
Recognizes human, mouse and rat APE1. Detects a band of ~37kDa by Western blot.
APPLICATION:
Immunocytochemistry
Immunohistochemistry (frozen sections, paraffin sections; human and rat only)
Western Blot
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
-20°C
Product Description
Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-.kappa.B, Myb, HIF-1α, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5' of an AP site to produce a hydroxyl group at the 3' terminus of an unmodified nucleotide upstream of the nick and a 5'-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase β to release 5'-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3'-5' DNA exonuclease activity, a 3'-phosphodiesterase activity, a 3'-phosphatase activity, and a RNase H activity. Recently it has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3' termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12.
Product Specific Literature References
The apurinic/apyrimidinic endonuclease (APE/ref-1) DNA repair enzyme is elevated in premalignant and malignant cervical cancer:
Y. Xu, et al.; Anticancer Res.
17
, 3713 (1997)
Abstract
Focal nuclear hepatocyte response to oxidative damage following low dose thioacetamide intoxication:
G.A. Clawson, et al.; Carcinogenesis
18
, 1663 (1997)
Abstract
Down-regulation of apurinic/apyrimidinic endonuclease expression is associated with the induction of apoptosis in differentiating myeloid leukemia cells:
K.A. Robertson, et al.; Cell Growth Differentiation
8
, 443 (1997)
Abstract
Differential cellular and subcellular expression of the human multifunctional apurinic/apyrimidinic endonuclease (APE/ref-1) DNA repair enzyme:
J.R. Duguid, et al.; Cancer Res.
55
, 6097 (1995)
Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
ALX-210-416
Revised 01-Nov-07
Polyclonal Antibody to Deoxyribonuclease I
SYNONYMS
anti-DNase I PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
DNA Endonucleases / Related Products
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-416-R100
100 µl
270.00 USD
