ALEXIS Biochemicals: Trichostatin A

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ALX-480-094

Revised 07-Oct-08

ADP-HPD . ammonium salt . dihydrate
SYNONYMS	Adenosine 5'-diphosphate (hydroxymethyl) pyrrolidinediol . NH₄ . 2H₂O
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Adenosine Derivatives Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-480-094-C060		60 µg	188.00 USD

Product Specification

FORMULA:	C₁₅H₂₃N₆O₁₂P₂ . NH₄ . 2H₂O
MW:	541.3 . 18.0 . 36.0
PURITY:	≥95% (HPLC)
APPEARANCE:	White to off-white amorphous solid.
SOLUBILITY:	Soluble in water (5mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C
HANDLING:	Protect from light. After reconstitution, prepare aliquots and store at -20°C. Packaged under inert gas. Hygroscopic.

Product Description

Potent, noncompetitive, and specific inhibitor of poly(ADP-ribose) glycohydrolase (PARG) (IC₅₀=120nM) versus ADP-ribose (IC₅₀=120µM). Does not affect the activities of either PARP-1 or NAD:arginine mono(ADP-ribosyl)-transferase A even at 1mM concentration.

Product Specific Literature References

Specific inhibition of poly(ADP-ribose) glycohydrolase by adenosine diphosphate (hydroxymethyl)pyrrolidinediol: J.T. Slama, et al.; J. Med. Chem. 38, 389 (1995) Abstract
Mechanism of inhibition of poly(ADP-ribose) glycohydrolase by adenosine diphosphate (hydroxymethyl)pyrrolidinediol: J.T. Slama, et al.; J. Med. Chem. 38, 4332 (1995) Abstract
A cellular defense pathway regulating transcription through poly(ADP-ribosyl)ation in response to DNA damage: S. Vispe, et al.; PNAS 97, 9886 (2000) Abstract; Full Text

Further Categories Containing This Product:

DNA Repair Other Products • PARG / Related Products

ALX-270-285

Revised 20-Oct-08

5-AIQ . hydrochloride
SYNONYMS	5-Aminoisoquinolinone . HCl
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	DNA Repair Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-285-M001		1 mg	50.00 USD
ALX-270-285-M005		5 mg	175.00 USD

Product Specification

FORMULA:	C₉H₈N₂O . HCl
MW:	160.2 . 36.5
PURITY:	≥97%
APPEARANCE:	Beige to brown powder.
SOLUBILITY:	Soluble in water (warm).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Protect from moisture.
HAZARD:	IRRITANT.

Product Description

Water-soluble, potent inhibitor of poly(ADP-ribose) polymerase-1 (PARP-1).

Product Specific Literature References

Effects of 5-aminoisoquinolinone, a water-soluble, potent inhibitor of the activity of poly (ADP-ribose) polymerase on the organ injury and dysfunction caused by haemorrhagic shock: M.C. McDonald, et al.; Br. J. Pharmacol. 130, 843 (2000) Abstract
Effects of 5-aminoisoquinolinone, a water-soluble, potent inhibitor of the activity of poly (ADP-ribose) polymerase, in a rodent model of lung injury: S. Cuzzocrea, et al.; Biochem. Pharmacol. 63, 293 (2002) Abstract
Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylation: H. Kleine, et al.; Mol. Cell 32, 57 (2008) Abstract

Further Categories Containing This Product:

PARP Inhibitors

ALX-270-044

Revised 20-Oct-08

3-Aminobenzamide
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-044-G001		1 g	50.00 USD
ALX-270-044-G005		5 g	190.00 USD

Product Specification

FORMULA:	C₇H₈N₂O
MW:	136.2
CAS NUMBER:	3544-24-9
RTECS:	CU8992000
PURITY:	≥97%
APPEARANCE:	White to tan powder.
SOLUBILITY:	Soluble in DMSO or 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C
HAZARD:	IRRITANT. MAY BE MUTAGENIC.

Product Description

Inhibitor of poly(ADP-ribose) polymerase-1 (PARP-1). Has minimal effect on bacterial toxin-mediated ADP-ribosylation. Apoptosis inhibitor.

Product Specific Literature References

Signal transduction in Coprinus congregatus: evidence for the involvement of G proteins in blue light photomorphogenesis: K.R. Kozak and I.A. Ross; BBRC 179, 1225 (1991) Abstract
Cell death protection by 3-aminobenzamide and other poly(ADP- ribose)polymerase inhibitors: different effects on human natural killer and lymphokine activated killer cell activities: D. Monti, et al.; BBRC 199, 525 (1994) Abstract
Cell death protection by 3-aminobenzamide: impairment of cytoskeleton function in human NK cell-mediated killing: W. Malorni, et al.; BBRC 199, 1250 (1994) Abstract
3-Aminobenzamide protects cells from UV-B-induced apoptosis by acting on cytoskeleton and substrate adhesion: W. Malorni, et al.; BBRC 207, 715 (1995) Abstract
Inactivation of the poly(ADP-ribose) polymerase gene affects oxygen radical and nitric oxide toxicity in islet cells: B. Heller, et al.; J. Biol. Chem. 270, 11176 (1995) Abstract
Inhibitors of poly(ADP-ribose) polymerase block nitric oxide-induced apoptosis but not differentiation in human leukemia HL-60 cells: M.L. Kuo, et al.; BBRC 219, 502 (1996) Abstract
Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylation: H. Kleine, et al.; Mol. Cell 32, 57 (2008) Abstract

Further Categories Containing This Product:

Apoptosis Inducers & Inhibitors Other Products • DNA Repair Other Products

ALX-270-250

Revised 18-Jan-06

4-Amino-1,8-naphthalimide
SYNONYMS	4-ANI
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-250-M010		10 mg	35.00 USD

Product Specification

FORMULA:	C₁₂H₈N₂O₂
MW:	212.2
CAS NUMBER:	1742-95-6
RTECS:	DE4081000
PURITY:	≥95%
APPEARANCE:	Yellow to orange solid.
SOLUBILITY:	Soluble in DMSO.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C
USE/STABILITY:	Stock solutions are stable for 3 months when stored at –20°C.
HAZARD:	IRRITANT.

Product Description

Potent inhibitor of poly(ADP-ribose) polymerase-1 (PARP-1) (IC₅₀=0.18µM). About 1000-fold more potent than 3-aminobenzamide (Prod. No. ALX-270-044).

Product Specific Literature References

Specific inhibitors of poly(ADP-ribose) synthetase and mono(ADP-ribosyl)transferase: M. Banasik, et al.; J. Biol. Chem. 267, 1569 (1992) Abstract; Full Text
Nitric oxide toxicity in islet cells involves poly(ADP-ribose) polymerase activation and concomitant NAD+ depletion: J. Radons, et al.; BBRC 199, 1270 (1994) Abstract
Combination effects of poly(ADP-ribose) polymerase inhibitors and DNA-damaging agents in ovarian tumor cell lines--with special reference to cisplatin: F. Bernges & W.J. Zeller; J. Cancer Res. Clin. Oncol. 122, 665 (1996) Abstract
Inhibition of the activity of poly(ADP ribose) synthetase reduces ischemia-reperfusion injury in the heart and skeletal muscle: C. Thiemermann, et al.; PNAS 94, 679 (1997) Abstract; Full Text
Effects of inhibitors of the activity of poly (ADP-ribose) synthetase on the liver injury caused by ischaemia-reperfusion: a comparison with radical scavengers: J. Bowes & C. Thiemermann; Br. J. Pharmacol. 124, 1254 (1998) Abstract
4-Amino-1,8-naphthalimide: a novel inhibitor of poly(ADP-ribose) polymerase and radiation sensitizer: A. Schlicker, et al.; Int. J. Radiat. Biol. 75, 91 (1999) Abstract

Further Categories Containing This Product:

DNA Repair Other Products

ALX-270-174

Revised 20-Oct-08

Benzamide
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	PARP Inhibitors

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-270-174-G005		5 g	20.00 USD

Product Specification

FORMULA:	C₇H₇NO
MW:	121.1
CAS NUMBER:	55-21-0
MERCK INDEX:	14: 1060
RTECS:	CU8700000
PURITY:	≥99%
APPEARANCE:	White to off-white solid.
SOLUBILITY:	Soluble in 100% ethanol.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C
HAZARD:	HARMFUL.

Product Description

Inhibitor of poly(ADP-ribose) polymerase-1 (PARP-1). Neuroprotectant.

Product Specific Literature References

Cytotoxicity of a new IMP dehydrogenase inhibitor, benzamide riboside, to human myelogenous leukemia K562 cells: H.N. Jayaram, et al.; BBRC 186, 1600 (1992) Abstract
Nitric oxide activation of poly(ADP-ribose) synthetase in neurotoxicity: J. Zhang, et al.; Science 263, 687 (1994) Abstract
Substrate-assisted catalysis by PARP10 limits its activity to mono-ADP-ribosylation: H. Kleine, et al.; Mol. Cell 32, 57 (2008) Abstract

Further Categories Containing This Product:

DNA Repair Other Products

ALX-550-322

Revised 11-Nov-08

Caffeine
SYNONYMS	1,3,7-Trimethylxanthine 3,7-Dihydro-1,3,7-trimethyl-1H-purine-2,6-dione
PRODUCT LINE	Natural Products / Antibiotics
PRODUCT CATEGORY	Natural Products for Neurological Research

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-550-322-G005		5 g	10.00 USD

Product Specification

FORMULA:	C₈H₁₀N₄O₂
MW:	194.2
CAS NUMBER:	58-08-2
MERCK INDEX:	14: 1636
SOURCE/HOST:	Found in tea leaves, coffee beans, cocoa beans, maté leaves, guarana paste and kola nuts.
PURITY:	≥99%
APPEARANCE:	White to colorless powder.
SOLUBILITY:	Soluble in chloroform, 100% alcohol or hot water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+20°C
HAZARD:	HARMFUL.
IDENTITY:	Identity determined by NIR.

Product Description

CNS stimulant. Blocks adenosine A1 and A2A receptors. cAMP phosphodiesterase inhibitor. Interferes with the uptake and storage of Ca2+ by the sarcoplasmic reticulum in skeletal muscle. Prevents apoptosis and cell cycle effects induced by various chemicals. Inhibits cellular DNA repair mechanisms. Anti-inflammatory.

Product Specific Literature References

Analogues of caffeine and theophylline: effect of structural alterations on affinity at adenosine receptors: J.W. Daly, et al.; J. Med. Chem. 29, 1305 (1986) Abstract
Caffeine as an analgesic adjuvant: a review of pharmacology and mechanisms of action: J. Sawynok & T.L. Yaksh; Pharmacol. Rev. 45, 43 (1993) Abstract
Caffeine prevents apoptosis and cell cycle effects induced by camptothecin or topotecan in HL-60 cells: F. Traganos, et al.; Cancer Res. 53, 4613 (1993) Abstract
Multiple effects of caffeine on calcium current in rat ventricular myocytes: I. Zahradnik & P. Palade; Pfluegers Arch. 424, 129 (1993) Abstract
Acute effects of caffeine on arterial stiffness, wave reflections, and central aortic pressures: T.G. Papaioannou, et al.; Am. J. Hypertens. 18, 129 (2005), (Review) Abstract
Caffeine and the dopaminergic system: O. Cauli & M. Morelli; Behav. Pharmacol. 16, 63 (2005), (Review) Abstract
Immunomodulatory effects of caffeine: friend or foe: L. A. Horrigan, et al.; Pharmacol. Ther. 111, 877 (2006), (Review) Abstract
Novel neuroprotection by caffeine and adenosine A(2A) receptor antagonists in animal models of Parkinson’s disease: A. Kalda, et al.; J. Neurol. Sci. 248, 9 (2006), (Review) Abstract
The enigmatic effects of caffeine in cell cycle and cancer: A. M. & Bode and Z. Dong; Cancer Lett. 247, 26 (2007), (Review) Abstract

Further Categories Containing This Product:

Natural Products - Other Signal Transduction Pathway Modulators • Ca2+ Modulators • Natural Products - Apoptosis Inducers & Inhibitors • Natural Products - Chemopreventive Agents • Neuroactive Agents Other Products • Active Substances from Fruit and Vegetables • Natural Products - Anti-inflammatory Agents • DNA Repair Other Products • Alkaloids • Other Natural Products - DNA Regulation / Transcription • Natural Products for Cell Cycle Research • Phosphodiesterases / Related Products

ALX-400-037

Revised 10-Apr-08

Carmustine
SYNONYMS	1,3-bis[2-Chloroethyl]-1-nitrosourea BCNU
PRODUCT LINE	Cancer
PRODUCT CATEGORY	Antitumor Agents (DNA Interaction & Gene Regulation)

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-400-037-M025		25 mg	52.00 USD

Product Specification

FORMULA:	C₅H₉CI₂N₃O₂
MW:	214.1
CAS NUMBER:	154-93-8
MERCK INDEX:	14: 1845
PURITY:	≥96% (Assay)
APPEARANCE:	Off-white to yellow crystalline solid.
SOLUBILITY:	Soluble in water (4mg/ml) or 50% ethanol (150mg/ml).
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HAZARD:	IRRITANT. MAY BE CARCINOGENIC.

Product Description

DNA alkylating agent causing DNA interstrand cross-linking. Inhibitor of glutathione reductase, lipoamide dehydrogenase and trypanothione reductase.

ALX-400-038	Lomustine
ALX-400-039	Semustine

SOURCE/HOST:	Purified from calf thymus and activated by digestion with DNAse I.
CONCENTRATION:	0.2mg/ml
PURITY:	≥99% (SDS-PAGE)
FORMULATION:	Liquid. In TRIS-EDTA.
APPLICATION:	For use with PARP-1 (Prod. No. ALX-201-063) or PARP-2 (Prod. No. ALX-201-064) in vitro activity tests. For details please see protocol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	+4°C

ALX-201-063	PARP-1 (human) (recombinant) (high purity)
ALX-201-064	PARP-2 (mouse) (recombinant) (high purity)

MW:	~102kDa. Note: The mobility of DNA ligase I in a PAA gel is not corresponding to its MW; it runs at ~125kDa.
SOURCE/HOST:	Produced in E. coli. Full length human DNA ligase I is fused at the N-terminus to a His-tag.
CONCENTRATION:	0.6mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM HEPES-KOH, pH 7.5, containing 30mM sodium chloride, 1mM EDTA, 1mM DTT and 30% glycerol.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-80°C
USE/STABILITY:	Enzyme is freshly produced upon customer request and is stable for ~2 weeks at -80°C. Very prone to degradation even in the absence of proteases. Freeze in liquid nitrogen for long term storage.
HANDLING:	After opening, prepare aliquots and store in liquid nitrogen. Avoid freeze/thaw cycles.

FORMULA:	C₁₅H₁₀ClN₃O
MW:	283.7
PURITY:	≥95% (HPLC)
APPEARANCE:	Light brown solid.
SOLUBILITY:	Soluble in DMSO (10mg/ml).
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
HANDLING:	Packaged under inert gas. Protect from light.
HAZARD:	IRRITANT.