ALEXIS Biochemicals: MDA5 (human) (IntraCellular) ELISA Kit

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ALX-746-022

Revised 11-Sep-08

Poly(deoxyadenosine:deoxythymidine) (endotoxin-free) (synthetic)
SYNONYMS	Poly(dA:dT) (endotoxin-free) (synthetic)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Antiviral Signalling / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-746-022-C050		50 µg	320.00 USD

Product Specification

SOURCE/HOST:	Synthetic.
QUANTITY:	Aliquoted by weight of dry material. Contains polymer, residual salt and water. Sufficient for at least 200 cellular activation assays at 0.025-0.25µg/200µl.
FORMULATION:	Lyophilized. Sterile.
ENDOTOXIN CONTENT:	<0.02EU/µg (LAL test; BioWhittaker)
RECONSTITUTION:	For a 250µg/ml stock solution, dissolve the total vial content in 200µl endotoxin-free buffer or water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	+4°C
USE/STABILITY:	Aqueous stock solution is stable for 1 day when stored at +4°C.
HANDLING:	For maximum product recovery after thawing, centrifuge the vial before opening the cap. After reconstitution, prepare aliquots and store at -20°C. Protect from light.

Product Description

Strong IFN inducer, independent of IRF3.

General Information

BACKGROUND/TECHNICAL INFORMATION

For in vitro stimulation we recommend the following procedure:
1. Bone marrow-derived mouse dendritic cells (BM mDC) are cultured 8x10⁴/100µl in RPMI containing 10% FCS over night at 5% CO₂ and 37°C.
2. The medium is removed and replaced by 100µl OPTI MEM (Invitrogen) and subsequently the transfection mixture is added: 25µl OPTI MEM containing 0.025 to 0.25 Poly(dA:dT) + 25µl OPTI MEM containing 0.5µl Lipofectamine 2000 (Invitrogen) or alternative transfection agents.
3. Over night stimulation at 5% CO₂ and 37°C.
4. Measurement of IFN-α/β in culture supernatants.

General Literature References

DAI (DLM-1/ZBP1) is a cytosolic DNA sensor and an activator of innate immune response : A. Takaoka, et al.; Nature 448, 501 (2007) Abstract
Cytosolic DNA recognition for triggering innate immune responses: A. Takaoka & T. Taniguchi; Adv. Drug Deliv. Rev. 60, 847 (2008) Abstract
Intracellular pattern-recognition receptors : C. Dostert, et al.; Adv. Drug Deliv. Rev. 60, 830 (2008) Abstract
The inflammasome recognizes cytosolic microbial and host DNA and triggers an innate immune response: D.A. Muruve, et al.; Nature 452, 103 (2008) Abstract

Related Products

ALX-746-021	Polyinosinic-polycytidylic acid . potassium salt (TLRgrade™) (synthetic)
ALX-505-007	PBS (endotoxin-free)
ALX-505-008	ddWater (endotoxin-free)
ALX-746-022	Poly(deoxyadenosine:deoxythymidine) (endotoxin-free) (synthetic)

ALX-201-221

Revised 02-Oct-06

Pin1 (human) (recombinant)
SYNONYMS	Peptidyl-prolyl cis-trans Isomerase Pin1 (human) (recombinant) PPIase Pin1 (human) (recombinant) Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 (human) (recombinant) PPIase NIMA-interacting 1 (human) (recombinant) Rotamase Pin1 (human) (recombinant)
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Alzheimer Research Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-221-C100		100 µg	325.00 USD

Product Specification

MW:	~18.2kDa
SOURCE/HOST:	Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1 produced in E. coli.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 20mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 5mM DTT and 20% glycerol.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

Further Categories Containing This Product:

Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Recombinant Proteins / Fusion Proteins • p53 Other Products

ALX-804-362

Revised 25-Sep-08

Monoclonal Antibody to Toll-like Receptor 3 (human) (40C1285.6)
SYNONYMS	anti-TLR3 MAb (40C1285.6) anti-CD283 (human) MAb (40C1285.6)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-362-C100		100 µg	293.00 USD
ALX-804-362B-C100	Biotin	100 µg	319.00 USD
ALX-804-362F-C100	FITC	100 µg	358.00 USD
ALX-804-362R-C100	R-PE	100 µg	397.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	40C1285.6
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	40C1285.6: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide. 40C1285.6-Biotin: Liquid. In PBS containing 0.02% sodium azide. 40C1285.6-FITC: Liquid. In 10mM TRIS containing 150mM sodium chloride and 0.02% sodium azide. 40C1285.6-R-PE: Liquid. In PBS containing 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptides corresponding to human TLR3 (Toll-like receptor 3) cytoplasmic portion.
SPECIFICITY:	Recognizes human TLR3.
APPLICATION:	Flow Cytometry (0.5-4µg/10⁶ cells for intracellular staining, for cell surface see [7]) Immunocytochemistry (see [3],[8] and [9]) Immunohistochemistry (paraffin sections) (see [4] and [6]) Immunoprecipitation (2µg/10⁶ transfected cells) Western Blot (1-3µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
HANDLING:	For long term storage keep unconjugated antibody at -20°C. Avoid freeze/thaw cycles. Keep Biotin-, FITC- and R-PE-conjugated formats at +4°C. Do not freeze. Protect from light.

Product Images

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Product Specific Literature References

[1] Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003) Abstract
[2] The effect of innate immunity on autoimmune diabetes and the expression of Toll-like receptors on pancreatic islets: L. Wen, et al.; J. Immunol. 172, 3173 (2004) Abstract
[3] Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses: T.A. Schneeman, et al.; J. Immunol. 175, 376 (2005) Abstract
[4] Toll-like receptors-2, -3 and -4 expression patterns on human colon and their regulation by mucosal-associated bacteria: E. Furrie, et al.; Immunology 115, 565 (2005) Abstract
[5] Distinct poly(I-C) and virus-activated signaling pathways leading to interferon-beta production in hepatocytes: K. Li, et al.; J. Biol. Chem. 280, 16739 (2005 280, 16739 (2005) Abstract; Full Text
[6] Thyrocytes express a functional toll-like receptor 3: overexpression can be induced by viral infection and reversed by phenylmethimazole and is associated with Hashimoto’s autoimmune thyroiditis: N. Harii, et al.; Mol. Endocrinol. 19, 1231 (2005) Abstract
[7] Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils: C.K. Wong, et al.; Am. J. Respir. Cell Mol. Biol. 37, 85 (2007) Abstract
[8] Biochemical and functional analyses of the human Toll-like receptor 3 ectodomain: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 7668 (2007) Abstract; Full Text
[9] Effects of single nucleotide polymorphisms on Toll-like receptor 3 activity and expression in cultured cells: C.T. Ranjith-Kumar, et al.; J. Biol. Chem. 282, 17696 (2007) Abstract; Full Text

Further Categories Containing This Product:

Antiviral Signalling / Related Products • Monoclonal Antibodies • CD Antibodies

ALX-804-372

Revised 22-May-08

Monoclonal Antibody to TANK-binding Kinase 1 (108A429)
SYNONYMS	anti-TBK1 MAb (108A429) anti-NAK MAb (108A429) anti-NF-κB-activating Kinase MAb (108A429) anti-T2K MAb (108A429)
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	IkB Kinases [IKKs] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-372-C100		100 µg	293.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Dog
CLONE:	108A429
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.5mg/ml
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN:	Synthetic peptide corresponding to aa 563-577 (YYQFKKDKAERRLAYC) of human TBK1 (TANK-binding kinase 1).
SPECIFICITY:	Recognizes human, mouse, rat, dog and bovine TBK1. Detects a band of ~80kDa by Western blot.
APPLICATION:	Western Blot (1-2µg/ml)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C

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Product Description

TANK-binding Kinase 1 (TBK1) is a member of the IKK family. TANK (TRAF-associated NF-κB activator) interacts with TBK1 and activates NF-κB in a kinase-dependent manner [1].

Product Specific Literature References

[1] NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase: J.L. Pomerantz & D. Baltimore; EMBO J. 18, 6694 (1999) Abstract; Full Text
[2] IKKepsilon and TBK1 are essential components of the IRF3 signaling pathway: K.A. Fitzgerald, et al.; Nat. Immunol. 4, 491 (2003) Abstract
[3] Regulating intracellular antiviral defense and permissiveness to hepatitis C virus RNA replication through a cellular RNA helicase, RIG-I: R. Sumpter, Jr., et al.; J. Virol. 79, 2689 (2005) Abstract; Full Text
[4] Gene expression and antiviral activity of alpha/beta interferons and interleukin-29 in virus-infected human myeloid dendritic cells: P. Osterlund, et al.; J. Virol. 79, 9608 (2005) Abstract

Further Categories Containing This Product:

Antiviral Signalling / Related Products • Monoclonal Antibodies

ALX-804-474

Revised 24-Mar-05

Monoclonal Antibody to Toll-like Receptor 3 (human) (TLR3.7)
SYNONYMS	anti-TLR3 (human) MAb (TLR3.7) anti-CD283 (human) MAb (TLR3.7)
PRODUCT LINE	Inflammation
PRODUCT CATEGORY	TLR Antibodies

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-474-C050		50 µg	220.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	TLR3.7
ISOTYPE:	Mouse IgG1
CONCENTRATION:	0.2mg/ml.
FORMULATION:	Liquid. Purified antibody in PBS containing 10% glycerol. Does not contain any preservatives.
IMMUNOGEN:	Recombinant human TLR3 (Toll-like receptor 3).
SPECIFICITY:	Recognizes human TLR3.
APPLICATION:	Flow Cytometry Immunocytochemistry Immunoprecipitation Functional Application (blocks double-stranded RNA-mediated signalling)
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored as recommended.
HANDLING:	Avoid freeze/thaw cycles.

Product Images

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Product Specific Literature References

Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling: M. Matsumoto, et al.; BBRC 293, 1364 (2002) Abstract
Subcellular localization of Toll-like receptor 3 in human dendritic cells: M. Matsumoto, et al.; J. Immunol. 171, 3154 (2003) Abstract
Mechanism of up-regulation of human Toll-like receptor 3 secondary to infection of measles virus-attenuated strains: M. Tanabe, et al.; BBRC 311, 39 (2003) Abstract

General Information

MANUFACTURER

Manufactured by Apotech Corporation.

Further Categories Containing This Product:

Antiviral Signalling / Related Products • Monoclonal Antibodies • CD Antibodies

ALX-804-495

Revised 22-Dec-06

Monoclonal Antibody to Pin1 (8C10)
SYNONYMS	anti-Peptidyl-prolyl cis-trans Isomerase Pin1 MAb (8C10) anti-PPIase Pin1 (8C10) MAb anti-Peptidyl-prolyl cis-trans Isomerase NIMA-interacting 1 MAb (8C10) anti-PPIase NIMA-interacting 1 (8C10) MAb anti-Rotamase Pin1 MAb (8C10)
PRODUCT LINE	Neurobiology
PRODUCT CATEGORY	Alzheimer Research Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-495-C100		100 µg	453.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Dog
CLONE:	8C10
ISOTYPE:	Mouse IgG1
PURITY DETAIL:	Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
FORMULATION:	Lyophilized from 1ml 2x PBS, 0.09% sodium azide, PEG and sucrose.
RECONSTITUTION:	Reconstitute with 1ml distilled water (15min, RT).
IMMUNOGEN:	Recombinant human peptidyl-prolyl cis-trans isomerase (PPIase) Pin1.
SPECIFICITY:	Recognizes human, mouse and dog Pin1. Detects a band of ~16 kDa by Western blot.
APPLICATION:	Western Blot (0.5µg/ml for HRPO/ECL detection). Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4'000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride.
SHIPPING:	SHIPPED ON BLUE ICE
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
HANDLING:	After reconstitution, prepare aliquots and freeze in liquid nitrogen. Avoid freeze/thaw cycles.
POSITIVE CONTROL INCLUDED:	Yes (Prod. No. ALX-840-030).

General Information

Pin1, a member of the parvulin family of peptidyl-prolyl-cis-trans-isomerases (PPIases), specifically isomerizes only the phosphorylated Ser/Thr-Pro bonds in certain proteins like Bcl-2, Cdc25C, Cdc27, p53 and Tau. Binding of Pin1 to p53 stimulates the DNA binding activity and transactivation function of p53. Pin1 also regulates the expression of cyclin D1 and is strikingly overexpressed in a subset of human tumors like breast cancer and oral squamous cell carcinoma.

Further Categories Containing This Product:

Stress & Heat Shock Proteins Other Products • Antiviral Signalling / Related Products • Monoclonal Antibodies • p53 Other Products

ALX-804-847

Revised 19-Jun-08

Monoclonal Antibody to Cardif (human) (Adri-1)
SYNONYMS	anti-CARD Adapter Inducing Interferon-β (human) MAb (Adri-1) anti-IPS-1 Cardif (human) MAb (Adri-1) anti-Interferon-β Promoter Stimulator Protein 1 (human) MAb (Adri-1) anti-MAVS (human) MAb (Adri-1) anti-Mitochondrial Antiviral Signalling Protein (human) MAb (Adri-1) anti-VISA (human) MAb (Adri-1) anti-Virus-induced Signalling Adapter (human) MAb (Adri-1)
PRODUCT LINE	Immunology
PRODUCT CATEGORY	Antiviral Signalling / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-847-C100		100 µg	370.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human
CLONE:	Adri-1
ISOTYPE:	Mouse IgG2b
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
SPECIFICITY:	Recognizes human Cardif.
APPLICATION:	Immunocytochemistry (1:100) Immunohistochemistry (paraffin sections) Immunoprecipitation (1:200) Western Blot (1:1’000)
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
USE/STABILITY:	Stable for at least 1 year after receipt when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.

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Product Specific Literature References

TRADD Protein Is an Essential Component of the RIG-like Helicase Antiviral Pathway: M.C. Michallet, et al.; Immunity 28, 651 (2008) Abstract

General Information

RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons.
Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.

MANUFACTURER

Manufactured by Apotech Corporation.

General Literature References

Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005) Abstract
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005) Abstract
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005) Abstract
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005) Abstract
CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006) Abstract

Further Categories Containing This Product:

NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Polyclonal Antibodies

ALX-804-849

Revised 16-Aug-07

Monoclonal Antibody to RIG-I (Alme-1)
SYNONYMS	anti-RIG-1 MAb (Alme-1) anti-Retinoic Acid-inducible Gene 1 Protein MAb (Alme-1) anti-DEAD-box Protein 58 MAb (Alme-1) anti-Probable ATP-dependent RNA Helicase DDX58 MAb (Alme-1)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	RNA Helicases / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-804-849-C100		100 µg	290.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse
CLONE:	Alme-1
ISOTYPE:	Mouse IgG1
SOURCE/HOST:	Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
PURITY DETAIL:	Protein G-affinity purified.
FORMULATION:	Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN:	Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY:	Recognizes human and mouse RI