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Apoptosis & Necrosis Detection Other Products
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ALX-380-283 Revised 21-May-08
7-Amino-actinomycin D
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SYNONYMS 7-AAD
7-Aminoactinomycin C1
PRODUCT LINE Natural Products / Antibiotics
PRODUCT CATEGORY Antitumor Antibiotics
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-380-283-M001   1 mg 90.00 USD Add To Cart
ALX-380-283-M005   5 mg 360.00 USD Add To Cart
Product Specification
FORMULA: C62H87N13O16
MW: 1270.4
CAS NUMBER: 7240-37-1
RTECS: AU1579000
SOURCE/HOST: Semisynthetic from actinomycin D (Prod. No. ALX-380-009).
PURITY: ≥96% (HPLC)
APPEARANCE: Red to dark purple powder.
SOLUBILITY: Soluble in DMSO (20mg/ml), methanol or 100% ethanol; slightly soluble in water.
SHIPPING: AMBIENT
LONG TERM STORAGE: -20°C
HANDLING: Protect from light. Hygroscopic.
HAZARD: VERY TOXIC.

Product Description
Membrane impermeable fluorescent DNA intercalator. Used as a fluorescent DNA stain. Inhibitor of DNA-primed RNA polymerase and DNA polymerase. Induces apoptosis and is used as a tool to identify apoptotic cells. Cytochemical probe. Exhibits growth-inhibitory activity against certain leukemias and sarcomas. Has antibacterial properties.
Product Specific Literature References
7-Amino-actinomycin D as a cytochemical probe. I. Spectral properties: J.E. Gill, et al.; J. Histochem. Cytochem. 23, 793 (1975) Abstract; Full Text
7-substituted actinomycin D analogs. Chemical and growth-inhibitory studies: S.K. Sengupta, et al.; J. Med. Chem. 18, 1175 (1975) Abstract
Simultaneous cell cycle analysis and two-color surface immunofluorescence using 7-amino-actinomycin D and single laser excitation: applications to study of cell activation and the cell cycle of murine Ly-1 B cells: P.S. Rabinovitch, et al.; J. Immunol. 136, 2769 (1986) Abstract
Binding specificities of actinomycin D to non-self-complementary -XGCY-tetranucleotide sequences: F.M. Chen; Biochemistry 31, 6223 (1992) Abstract
Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry: I. Schmid, et al.; Cytometry 13, 204 (1992) Abstract
Improved staining method for the simultaneous flow cytofluorometric analysis of DNA content, S-phase fraction, and surface phenotype using single laser instrumentation: K. Toba, et al.; Cytometry 13, 60 (1992) Abstract
Flow cytometric immunofluorescence assay for quantification of cyclobutyldithymine dimers in separate phases of the cell cycle: R.J. Berg, et al.; Carcinogenesis 14, 103 (1993) Abstract
Simultaneous three-color analysis of the surface phenotype and DNA-RNA quantitation using 7-amino-actinomycin D and pyronin Y: K. Toba, et al.; J. Immunol. Methods 182, 193 (1995) Abstract
Hematopoietic cell protein-tyrosine phosphatase-deficient motheaten mice exhibit T cell apoptosis defect: X. Su, et al.; J. Immunol. 156, 4198 (1996) Abstract
The use of 7-amino actinomycin D in identifying apoptosis: simplicity of use and broad spectrum of application compared with other techniques: N.J. Philpott, et al.; Blood 87, 2244 (1996) Abstract; Full Text
A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry: I. Schmid, et al.; J. Immunol. Methods 170, 145 (1996) Abstract
Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods: H. Lecoeur, et al.; J. Immunol. Methods 209, 111 (1997) Abstract
Cell kinetic study of normal human bone marrow hematopoiesis and acute leukemia using 7AAD/PY: K. Toba, et al.; Eur. J. Haematol. 64, 10 (2000) Abstract
Live-cell assay for detection of apoptosis by dual-laser flow cytometry using Hoechst 33342 and 7-amino-actinomycin D: I. Schmid, et al.; Nat. Protoc. 2, 187 (2007) Abstract
Related Products
 
 
ALX-850-248 Revised 22-Apr-08
ApoSENSOR™ ADP/ATP Ratio Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apoptosis & Necrosis Detection Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-248-KI01   200 tests 455.00 USD Add To Cart
Product Specification
QUANTITY: 200 assays.
KIT/SET CONTAINS: 50ml Nucleotide Releasing Buffer, 1 vial ATP Monitoring Enzyme, 1 vial ADP Converting Enzyme, 500µl Enzyme Reconstitution Buffer.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Protect from light.
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Product Description
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
The assay utilizes bioluminescent detection of ADP and ATP levels for rapid screening of apoptosis, necrosis, growth arrest and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light fom ATP and luciferin. The light can be measured using a luminometer or beta counter. ADP levels are measured by its conversion to ATP that is subsequently detected using the same reaction.
This highly sensitive assay (detects 100 mammalian cells/well) can be fully automated for high throughput measurements. The ADP/ATP ratio assay offers highly consistent results, with excellent correlation to other apoptosis markers (e. g. TUNEL-based techniques and caspase assays).
General Information
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Quantification Kits
 
 
ALX-850-247 Revised 22-Apr-08
ApoSENSOR™ Cell Viability Assay Kit
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Apoptosis & Necrosis Detection Other Products
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-850-247-KI01   200 tests 203.00 USD Add To Cart
ALX-850-247-KI02   1000 tests 609.00 USD Add To Cart
Product Specification
KIT/SET CONTAINS: Nucleotide Releasing Buffer, ATP Monitoring Enzyme, Enzyme Reconstitution Buffer, ATP.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Protect from light.
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Product Description
The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
General Information
MANUFACTURER Manufactured by BioVision.
Further Categories Containing This Product:
Quantification Kits
 
 
ALX-610-046 Revised 02-Mar-06
DiOC6(3)
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SYNONYMS 3,3'-Dihexyloxacarbocyanine iodide
PRODUCT LINE Other Products
PRODUCT CATEGORY Dyes / Stains / Fluorescent Probes / Fluorescent Labels
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ALX-610-046-M050   50 mg 50.00 USD Add To Cart
Product Specification
FORMULA: C29H37IN2O2
MW: 572.5
CAS NUMBER: 532123-82-4
PURITY: ≥98%
APPEARANCE: Red to violet crystalline solid.
SOLUBILITY: Soluble in 100% ethanol.
SHIPPING: AMBIENT
LONG TERM STORAGE: +4°C
HANDLING: Keep under inert gas. Protect from light.
HAZARD: IRRITANT.
Product Description
Fluorescent probe (Em(max): 501nm) that selectively stains mitochondria and the endoplasmatic reticulum. Electron transport inhibitor and useful probe for studying multidrug resistant cells.
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Apoptosis & Necrosis Detection Other Products
 
 
ALX-804-305 Revised 10-Nov-08
Monoclonal Antibody to Caspase-3 (31A1067)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Antibodies
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-305-C100   100 µg 293.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
CLONE: 31A1067
ISOTYPE: Mouse IgG1
CONCENTRATION: 0.5mg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.05% BSA and 0.05% sodium azide.
IMMUNOGEN: Recombinant human caspase-3.
SPECIFICITY: Recognizes human, mouse and rat procaspase-3 and cleaved 12 and 17kDa catalytic subunits of human caspase-3.
APPLICATION: Immunohistochemistry (frozen sections (see [4]), paraffin sections (see [18]))
Western Blot (2-3µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for 6 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
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Product Specific Literature References
[1] Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax: S.M. Cuddeback, et al.; J. Biol. Chem. 276, 20559 (2001) Abstract; Full Text
[2] The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change: H. Yamaguchi and H.G. Wang; Oncogene 20, 7779 (2001) Abstract
[3] Identification of retinamides that are more potent than N-(4-hydroxyphenyl)retinamide in inhibiting growth and inducing apoptosis of human head and neck and lung cancer cells: S.Y. Sun, et al.; Cancer Epidemiol. Biomarkers Prev. 10, 595 (2001) Abstract; Full Text
[4] Therapeutic time window of adenovirus-mediated GDNF gene transfer after transient middle cerebral artery occlusion in rat: W.R. Zhang, et al.; Brain Res. 947, 140 (2002) Abstract
[5] The synthetic retinoid CD437 selectively induces apoptosis in human lung cancer cells while sparing normal human lung epithelial cells: S.Y. Sun, et al.; Cancer Res. 62, 2430 (2002) Abstract; Full Text
[6] Identification of a novel synthetic triterpenoid, methyl-2-cyano-3,12-dioxooleana-1,9-dien-28-oate, that potently induces caspase-mediated apoptosis in human lung cancer cells: K.B. Kim, et al.; Mol. Cancer Ther. 1, 177 (2002) Abstract; Full Text
[7] Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production: Y.C. Chen, et al.; Biochem. Pharmacol. 64, 1713 (2002) Abstract
[8] Reversal of Taxol resistance in hepatoma by cyclosporin A: involvement of the PI-3 kinase-AKT 1 pathway: H.L. Lin, et al.; Br. J. Cancer 88, 973 (2003) Abstract
[9] Apoptotic proteins in the temporal cortex in schizophrenia: high Bax/Bcl-2 ratio without caspase-3 activation: L.F. Jarskog, et al.; Am. J. Psychiatry 161, 109 (2004) Abstract
[10] Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3: Y.H. Wu, et al.; J. Biol. Chem. 279, 19264 (2004) Abstract; Full Text
[11] Ricin triggers apoptotic morphological changes through caspase-3 cleavage of BAT3: Y.H. Wu, et al.; J. Biol. Chem. 279, 19264 (2004) Abstract; Full Text
[12] Hydroxylation at C4’ or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species: C.H. Ko, et al.; Free Radic. Biol. 36, 897 (2004) Abstract
[13] Overexpression of Bcl-2 is associated with apoptotic resistance to the G-quadruplex ligand 12459 but is not sufficient to confer resistance to long-term senescence: C. Douarre, et al.; Nucl. Acids Res. 33, 2192 (2005) Abstract; Full Text
Synergism of Rana catesbeiana ribonuclease and IFN-gamma triggers distinct death machineries in different human cancer cells: C.H. Tang, et al.; FEBS Lett. 579, 265 (2005) Abstract
[14] Justicidin A decreases the level of cytosolic Ku70 leading to apoptosis in human colorectal cancer cells: J.C. Lee, et al.; Carcinogenesis 26, 1716 (2005) Abstract
[15] Induction of endoplasmic reticulum stress and apoptosis by a marine prostanoid in human hepatocellular carcinoma: P.C. Chiang, et al.; J. Hepatol. 43, 679 (2005) Abstract
[16] Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells: F. Jin, et al.; Cancer Res. 65, 6354 (2005) Abstract
[17] Induction of endothelial cell apoptosis by lipid hydroperoxide-derived bifunctional electrophiles: W. Jian, et al.; Free Radic. Biol. Med. 39, 1162 (2005) Abstract
[18] Protein expression profiling and molecular classification of gastric cancer by the tissue array method: H.S. Lee, et al.; Clin. Cancer Res. 13, 4154 (2007) Abstract
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other ProductsMonoclonal Antibodies
 
 
ALX-804-210 Revised 21-Aug-08
Monoclonal Antibody to PARP-1 (C2-10)
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-1 MAb (C2-10)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-210-R050   50 µl 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
Others
CLONE: C2-10
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites fluid containing 0.02% sodium azide.
IMMUNOGEN: Purified calf thymus PARP-1 (poly(ADP-ribose) polymerase-1).
SPECIFICITY: Recognizes an epitope in the C-terminal part of the DNA binding domain of human, mouse, rat, hamster and primate PARP-1. Detects bands ~116kDa (intact PARP-1) and ~85kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with chicken PARP-1.
APPLICATION: ELISA: Titer: 1:1’000’000. Dilution buffer: PBS, pH 7.4, 1% non fat dried milk, 0.05% Tween 20.
Immunocytochemistry: 1:1’000-1:5’000. Dilution buffer: 50mM TRIS, pH 7.5, 150mM NaCl, 5% milk. For other conditions, see [1].
Immunoprecipitation: 1:100. Dilution buffer: RiPA buffer, Protein G Sepharose.
Western Blot: 1:10’000. Dilution buffer: PBS, pH 7.4, 5% non fat dried milk, 0.1% Tween 20. See also separate Western blot protocol.
Note: Does not neutralize the biological activity of PARP.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
[1] Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988) Abstract
[2] Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res. 53, 3976 (1993) Abstract
[3] Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994) Abstract
[4] Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995) Abstract; Full Text
[5] FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem. 271, 4961 (1996) Abstract; Full Text
[6] Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta 1334, 65 (1997) Abstract
[7] Selective loss of poly(ADP-ribose) and the 85-kDa fragment of poly(ADP- ribose) polymerase in nucleoli during alkylation-induced apoptosis of HeLa cells: R. Alvarez-Gonzalez, et al.; J. Biol. Chem. 274, 32122 (1999) Abstract; Full Text
[8] Inactivation of p21WAF1 sensitizes cells to apoptosis via an increase of both p14ARF and p53 levels and an alteration of the Bax/Bcl-2 ratio: D. Javelaud & F. Besançon; J. Biol. Chem. 277, 37949 (2002) Abstract; Full Text
[9] Nuclear poly(ADP-ribose) polymerase-1 rapidly triggers mitochondrial dysfunction: G. Cipriani, et al.; J. Biol. Chem. 280, 17227 (2005) Abstract; Full Text
CCCTC-binding Factor Activates PARP-1 Affecting DNA Methylation Machinery: T. Guastafierro, et al.; J. Biol. Chem. 283, 21873 (2008) Abstract; Full Text
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other ProductsMonoclonal Antibodies
 
 
ALX-804-211 Revised 06-Feb-08
Monoclonal Antibody to PARP-1 (F1-23)
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SYNONYMS anti-Poly(ADP-ribose) Polymerase-1 MAb (F1-23)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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Product Numbers: Format: Size: Unit Price: Quantity: Add To Cart
ALX-804-211-R050   50 µl 380.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
CLONE: F1-23
ISOTYPE: Mouse IgG1
FORMULATION: Liquid. Ascites fluid containing 0.02% sodium azide.
IMMUNOGEN: Purified bovine PARP-1 (poly(ADP-ribose) polymerase-1).
SPECIFICITY: Recognizes human and bovine PARP-1. The epitope is in the zinc finger II DNA binding domain of PARP-1. Detects bands of ~116kDa (intact PARP-1) and ~24kDa (apoptosis-induced cleavage fragment) by Western blot. Does not cross-react with mouse or rat PARP-1.
APPLICATION: ELISA:  >1:200’000. Dilution buffer: PBS, pH 7.4, 1% non fat dried milk, 0.05% Tween 20. Detectable antigen range: 50ng of PARP-1
Immunocytochemistry: 1:500. Dilution buffer: 50mM TRIS, pH 7.5, 150mM NaCl, 5% milk.
Immunoprecipitation: 1:50. Dilution buffer: RiPA buffer Protein G-Sepharose
Western Blot: 1:5’000. Dilution buffer: PBS, pH 7.4, 5% non fat dried milk, 0.1% Tween 20. See also separate Western blot protocol
Functional Application: Neutralizes the biological activity of PARP (~80%).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and store at -80°C.
Product Specific Literature References
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study: D. Lamarre, et al.; Biochim. Biophys. Acta 950, 147 (1988) Abstract
Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis: S.H. Kaufmann, et al.; Cancer Res. 53, 3976 (1993) Abstract
Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE: Y.A. Lazebnik, et al.; Nature 371, 346 (1994) Abstract
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution: Y.A. Lazebnik, et al.; PNAS 92, 9042 (1995) Abstract; Full Text
FADD/MORT1 is a common mediator of CD95 (Fas/APO-1) and tumor necrosis factor receptor-induced apoptosis: A.M. Chinnaiyan, et al.; J. Biol. Chem. 271, 4961 (1996) Abstract; Full Text
Characterization of anti-peptide antibodies directed towards the automodification domain and apoptotic fragment of poly (ADP-ribose) polymerase: P.J. Duriez, et al.; Biochim. Biophys. Acta 1334, 65 (1997) Abstract
PARP-3 localizes preferentially to the daughter centriole and interferes with the G1/S cell cycle progression: A. Augustin, et al.; J. Cell Sci. 116, 1551 (2003) Abstract
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other ProductsMonoclonal Antibodies
 
 
ALX-210-806 Revised 20-Mar-06
Polyclonal Antibody to Caspase-3
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Antibodies
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ALX-210-806-C100   100 µg 273.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.2mg/ml
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.2, containing 1% BSA, 0.02% thimerosal and 50% glycerol.
IMMUNOGEN: Synthetic peptide corresponding to aa 163-175 of human caspase-3.
SPECIFICITY: Recognizes human, mouse and rat caspase-3. Detects bands of ~32kDa (procaspase-3) and ~20kDa (cleavage product) by Western blot.
APPLICATION: Western Blot (1µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
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Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other ProductsPolyclonal Antibodies
 
 
ALX-210-807 Revised 22-Oct-07
Polyclonal Antibody to Caspase-3 (active)
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PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY Caspase Antibodies
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ALX-210-807-C100   100 µg 371.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
Rat
SOURCE/HOST: From rabbit.
CONCENTRATION: 0.2mg/ml.
PURITY DETAIL: Epitope-affinity purified.
FORMULATION: Liquid. In PBS containing 50% glycerol, 1% BSA and 0.02% thimerosal.
IMMUNOGEN: Synthetic peptide around the cleavage site of human caspase-3.
SPECIFICITY: Recognizes human, mouse and rat active caspase-3 (p20 subunit). Does not cross-react with procaspase-3 (~32kDa).
APPLICATION: Immunohistochemistry (paraffin sections (10-20µg/ml))
Western Blot (0.5-4µg/ml)
Optimal conditons must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-153-062.