ALEXIS Biochemicals: Polyclonal Antibody to 8-Hydroxyguanosine

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ALX-480-075

Revised 14-Jun-06

8-Hydroxyguanosine
SYNONYMS	8-OHG
PRODUCT LINE	Signal Transduction
PRODUCT CATEGORY	Guanosine Derivatives Other Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-480-075-M001		1 mg	30.00 USD
ALX-480-075-M005		5 mg	120.00 USD

Product Specification

FORMULA:	C₁₀H₁₃N₅O₆
MW:	299.2
CAS NUMBER:	3868-31-3
PURITY:	≥98%
APPEARANCE:	White to off-white powder.
SOLUBILITY:	Soluble in conc. NaOH. Once solubilized it can be further diluted and neutralized with water.
SHIPPING:	AMBIENT
LONG TERM STORAGE:	-20°C

Product Description

Oxidized form of the nucleoside guanosine. Formed in vivo by the free radical oxidation of RNA molecules, with subsequent degradation and cleavage of the nucleic acid.

Product Specific Literature References

8-Substituted guanosine and 2'-deoxyguanosine derivatives as potential inducers of the differentiation of Friend erythroleukemia cells: T.S. Lin, et al.; J. Med. Chem. 28, 1194 (1985) Abstract
Hydrolytic elimination of a mutagenic nucleotide, 8-oxodGTP, by human 18-kilodalton protein: sanitization of nucleotide pool: J.Y. Mo, et al.; PNAS 89, 11021 (1992) Abstract
Involvement of oxidative stress in motorcycle exhaust particle-induced DNA damage and inhibition of intercellular communication: M.L. Kuo, et al.; Mutat. Res. 413, 143 (1998) Abstract
Levels of 8-hydroxydeoxyguanosine as a marker of DNA damage in human leukocytes: M. Lodovici, et al.; Free Radic. Biol. Med. 28, 13 (2000) Abstract

Further Categories Containing This Product:

Nucleic Acid Oxidation & Alkylation / Related Products

ALX-201-258

Revised 18-Mar-08

Lysyl Oxidase-like Protein 4 (human) (recombinant) (His)
SYNONYMS	LOXL4 (human) (recombinant) (His)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Nucleic Acid Oxidation & Alkylation / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-258-C100		100 µg	390.00 USD

Product Specification

MW:	~65kDa
SOURCE/HOST:	Produced in E. coli. Human lysyl oxidase-like protein 4 (LOXL4) (aa 159-756) is fused to a His-tag.
CONCENTRATION:	~0.33mg/ml after reconstitution.
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Lyophilized from 50mM HEPES, pH 8.0, containing 200mM sodium chloride.
RECONSTITUTION:	Reconstitute with 300µl of 10% glycerol containing 2mM β-mercaptoethanol.
SPECIFIC ACTIVITY:	0.02-0.03µmol/min/µg
APPLICATION:	In vitro conjugation assays by monitoring benzylamine oxidation at A_250mm. Typical concentration to support conjugation in vitro is 0.5-2.5µM depending on conditions.
SHIPPING:	SHIPPED ON DRY ICE
LONG TERM STORAGE:	-80°C
HANDLING:	Avoid freeze/thaw cycles.

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Product Specific Literature References

A novel human lysyl oxidase-like gene (LOXL4) on chromosome 10q24 has an altered scavenger receptor cysteine rich domain: L. Asuncion, et al.; Matrix Biol. 20, 487 (2001) Abstract
Cloning and characterization of a fifth human lysyl oxidase isoenzyme: the third member of the lysyl oxidase-related subfamily with four scavenger receptor cysteine-rich domains: J.M. Maki, et al.; Matrix Biol. 20, 493 (2001) Abstract
Lysyl oxidases: a novel multifunctional amine oxidase family: K. Csiszar; Prog. Nucl. Acid Res. Mol. Biol. 70, 1 (2001) Abstract
Molecular cloning and biological activity of a novel lysyl oxidase-related gene expressed in cartilage: H. Ito, et al.; J. Biol. Chem. 276, 24023 (2001) Abstract; Full Text
A molecular role for lysyl oxidase in breast cancer invasion: D.A. Kirschmann, et al.; Cancer Res. 62, 4478 (2002) Abstract; Full Text
Purification of enzymatically active human lysyl oxidase and lysyl oxidase-like protein from Escherichia coli inclusion bodies: S.T. Jung, et al.; Protein Expr. Purif. 31, 240 (2003) Abstract
Expression and purification of enzymatically active forms of the human lysyl oxidase-like protein 4: M.S. Kim, et al.; J. Biol. Chem. 278, 52071 (2003) Abstract; Full Text
Structural and functional diversity of lysyl oxidase and the LOX-like proteins: J. Molnar, et al.; Biochim. Biophys. Acta 1647, 220 (2003) Abstract
Lysyl oxidase is essential for hypoxia-induced metastasis: J.T. Erler, et al.; Nature 440, 1222 (2006) Abstract
Selective upregulation and amplification of the lysyl oxidase like-4 (LOXL4) gene in head and neck squamous cell carcinoma: T. Gorogh, et al.; J. Pathol. 212, 74 (2007) Abstract
LOXL1 and LOXL4 are epigenetically silenced and can inhibit ras/extracellular signal-regulated kinase signaling pathway in human bladder cancer: G. Wu, et al; Cancer Res. 67, 4123 (2007) Abstract

General Information

BACKGROUND/TECHNICAL INFORMATION

Swiss-Prot link Q96JB6: Lysyl Oxidase-like Protein 4 (human)

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins

ALX-210-802

Revised 06-Dec-06

Polyclonal Antibody to 8-Hydroxyguanosine
PRODUCT LINE	Oxidative Stress
PRODUCT CATEGORY	Oxidative Stress Markers & Reagents / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-210-802-R100		100 µl	210.00 USD

Product Specification

SOURCE/HOST:	From goat.
FORMULATION:	Liquid. Neat serum containing 0.1% sodium azide.
IMMUNOGEN:	Synthetic 8-hydroxyguanosine-conjugate.
SPECIFICITY:	Recognizes 8-hydroxyguanosine and 8-hydroxy-2'-deoxyguanosine. Partially cross-reacts with 8-mercaptoguanosine and slightly with 8-bromoguanosine. Does not cross-react with other naturally occuring nucleotides.
APPLICATION:	ELISA (1:250'000) Immunohistochemistry (frozen sections, paraffin sections (1:200))
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging and neurodegenerative conditions, such as Alzheimer's disease. 8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.

Further Categories Containing This Product:

Nucleic Acid Oxidation & Alkylation / Related Products • Polyclonal Antibodies

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