ALEXIS Biochemicals: Proliferating Cell Nuclear Antigen (human) (recombinant)

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ALX-801-091

Revised 29-Sep-08

Monoclonal Antibody to Proliferating Cell Nuclear Antigen (PC10)
SYNONYMS	anti-PCNA MAb (PC10)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Proliferating Cell Nuclear Antigen [PCNA] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-801-091-L001		1 ml	480.00 USD

Product Specification

SPECIES CROSSREACTIVITY:	Human Mouse Rat Goat Sheep Dog Pig Monkey Others
CLONE:	PC10
ISOTYPE:	Mouse IgG2a
FORMULATION:	Liquid. In 0.01M PBS containing 0.1% sodium azide and 1% BSA.
IMMUNOGEN:	Recombinant rat PCNA (proliferating cell nuclear antigen).
SPECIFICITY:	Recognizes PCNA from all vertebrate species, insects and S. pombe. Intense nuclear staining of proliferating cells in normal and tumor tissues.
APPLICATION:	Immunohistochemistry (paraffin sections): Can be used for formalin-fixed, paraffin-embedded tissues. Pretreatment is not required. Recommended dilution is ≥1:40 for biotin based detection. For optimal staining, incubate 20-60 min. at room temperature. Optimal conditions must be determined individually.
SHIPPING:	SHIPPED ON BLUE ICE
SHORT TERM STORAGE:	+4°C
LONG TERM STORAGE:	-20°C
HANDLING:	Avoid freeze/thaw cycles.

General Information

Proliferating Cell Nuclear Antigen (PCNA) is associated with the DNA replication fork and its major function is described as a processivity factor for replicative DNA polymerases (polymerases δ and ε ) acting as a sliding clamp. It has a highly conserved amino acid sequence and has been identified in all eukaryotes and is being used as a proliferating marker. PCNA is also able to interact with multiple partners involved in Okazaki fragment joining, DNA repair, DNA methylation and chromatin assembly. In DNA repair PCNA has been reported not only for nucleotide excision repair (NER) (in concert with XPG and ERCC1), but also for base excision repair (BER) (binding to uracil-DNA glycosylase) and mismatch repair (MMR) (binding to mismatch repair enzymes MSH2 & MLH1). It is further associated with cell cycle control through cdk-cyclins during G1 to S phase.

Further Categories Containing This Product:

Monoclonal Antibodies • Tumor Markers

ALX-201-234

Revised 07-Nov-07

Proliferating Cell Nuclear Antigen (human) (recombinant)
SYNONYMS	PCNA (human) (recombinant)
PRODUCT LINE	DNA Regulation / Transcription
PRODUCT CATEGORY	Proliferating Cell Nuclear Antigen [PCNA] / Related Products

Ordering Information

Product Numbers:	Format:	Size:	Unit Price:	Quantity:	Add To Cart
ALX-201-234-C025		25 µg	240.00 USD

Product Specification

MW:	87kDa (homotrimer of 29kDa)
SOURCE/HOST:	Produced in E. coli.
CONCENTRATION:	1mg/ml
PURITY:	≥95% (SDS-PAGE)
FORMULATION:	Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 50mM sodium chloride, 1mM DTT and 1mM EDTA.
SHIPPING:	SHIPPED ON DRY ICE
SHORT TERM STORAGE:	-20°C
LONG TERM STORAGE:	-80°C
USE/STABILITY:	Stable for at least 4 weeks when stored at -20°C.
HANDLING:	After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.

Product Specific Literature References

The solution structure of functionally active human proliferating cell nuclear antigen determined by small-angle neutron scattering: P. Schurtenberger, et al.; J. Mol. Biol. 275, 123 (1998) Abstract

General Literature References

Proliferating cell nuclear antigen (PCNA): a dancer with many partners: G. Maga and U. Hubscher; J. Cell Sci. 116, 3051 (2003) Abstract

Further Categories Containing This Product:

Recombinant Proteins / Fusion Proteins • Tumor Markers

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