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ALX-201-063
Revised 25-Oct-06
PARP-1 (human) (recombinant) (high purity)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-063-C020
20 µg
348.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Produced in Sf9 cells.
CONCENTRATION:
1mg/ml.
PURITY:
≥99% (SDS-PAGE
PURITY DETAIL:
Affinity purified.
FORMULATION:
Liquid. In 100mM TRIS-HCl, pH 7.5,containing 14mM β-mercaptoethanol, 0.5mM EDTA, 0.5mM PMSF and 10% glycerol.
SPECIFIC ACTIVITY:
≥600U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min. at 25°C, pH 7.5.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
Product Specific Literature References
Overproduction and large-scale purification of the human poly(ADP- ribose) polymerase using a baculovirus expression system:
H. Giner, et al.; Gene
114
, 279 (1992)
Abstract
Poly(ADP-ribose) reactivates stalled DNA topoisomerase I and Induces DNA strand break resealing
:
M. Malanga & F.R. Althaus; J. Biol. Chem.
279
, 5244 (2004)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Related Products
ALX-840-040
DNA (purified) (DNAse I activated)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-232
Revised 18-Mar-08
PARP-1 (Catalytic Domain) (human) (recombinant) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (Catalytic Domain) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-201-232-C020
20 µg
380.00 USD
Product Specification
SOURCE/HOST:
Produced in SF21 cells. Human PARP-1 (aa 656-1014) is fused to a His-tag.
CONCENTRATION:
1.5mg/ml.
PURITY:
≥90% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM sodium chloride, 250mM imidazole, 2mM β-mercaptoethanol, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-250
Revised 18-Mar-08
PARP-1 (human) (recombinant) (high purity) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-250-C010
10 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length PARP-1 is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥98% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY:
≥1’200U/mg protein.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1:
P.O. Hassa, et al.; J. Biol. Chem.
278
, 45145 (2003)
Abstract
;
Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription:
P.O. Hassa, et al.; J. Biol. Chem.
280
, 40450 (2005)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-253
Revised 18-Mar-08
PARP-1b (human) (recombinant) (active) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1b (human) (recombinant) (active) (His)
short-form PARP-1 (human) (recombinant) (active) (His)
sPARP-1 (human) (recombinant) (active) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-253-C020
20 µg
240.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human PARP-1b (short-form PARP1; sPARP-1) is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Characterization of sPARP-1. An alternative product of PARP-1 gene with poly(ADP-ribose) polymerase activity independent of DNA strand breaks:
F.R. Sallmann, et al.; J. Biol. Chem.
275
, 15504 (2000)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
General Literature References
Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going?:
P.O. Hassa, et al.; Microbiol. Mol. Biol. Rev.
70
, 789 (2006)
Abstract
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-254
Revised 18-Mar-08
PARP-1 (E988K mutant) (human) (recombinant) (Control)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (E988K mutant) (human) (recombinant) (Control)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
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ALX-201-254-C010
10 µg
180.00 USD
Product Specification
SOURCE/HOST:
Produced in Sf21 cells. Human full-length inactive mutant E988K of PARP-1 is fused to a His-tag.
CONCENTRATION:
0.5mg/ml.
PURITY:
≥95% (SDS-PAGE)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY:
0.5% of wild type PARP-1.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function:
P.O. Hassa, et al.; J. Biol. Chem.
276
, 45588 (2001)
Abstract
;
Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-201-255
Revised 18-Mar-08
PARP-1 (BRCT Domain) (human) (recombinant) (His)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (BRCT Domain) (human) (recombinant) (His)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-201-255-C020
20 µg
290.00 USD
Product Specification
SOURCE/HOST:
Produced in
E. coli
. Human PARP-1 (BRCT domain) (aa 385-524) is fused to a His-tag.
CONCENTRATION:
1mg/ml
PURITY:
≥99% (SDS-PAGE)
PURITY DETAIL:
Affinity purified on Ni-beads.
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 8, containing 200mM sodium chloride, 60mM imidazole, 0.5mM PMSF and 10% glycerol.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Images
Please click on thumbnails to enlarge.
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P09874:
PARP-1 (human)
Related Products
ALX-210-540
Polyclonal Antibody to PARP-1 (BRCT Domain)
Further Categories Containing This Product:
Enzymes
•
Recombinant Proteins / Fusion Proteins
ALX-202-042
Revised 16-Apr-08
PARP-1 (bovine) (~90%)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (bovine) (~90%)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
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ALX-202-042-C010
10 µg
180.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Isolated from calf thymus.
CONCENTRATION:
335μg/ml
PURITY:
~90% (SDS-PAGE, Western blot)
FORMULATION:
Liquid. In 60mM kalium phosphate, pH 7.2, containing 0.5 - 2M kalium chloride, 10% glycerol, 1mM DTT, 10mM β−mercaptoethanol, 0.1mM 0.1 PMSF.
SPECIFIC ACTIVITY:
500-700U/mg protein.
One unit is defined as the amount of enzyme that synthesizes 1nmol poly(ADP-ribose) per min. at 25°C.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Do not freeze/thaw.
Product Specific Literature References
Erasable blot of poly(ADP-ribose) polymerase:
S. Desnoyers, et al.; Anal. Biochem.
218
, 470 (1994)
Abstract
Autoantibodies reacting with poly(ADP-ribose) and with a zinc-finger functional domain of poly(ADP-ribose) polymerase involved in the recognition of damaged DNA:
S. Muller, et al.; Clin. Immunol. Immunopathol.
73
, 187 (1994)
Abstract
Characterization of the execution phase of apoptosis in vitro using extracts from condemned-phase cells:
Y.A. Lazebnik, et al.; J. Cell Sci. Suppl.
19
, 41 (1995)
Abstract
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution:
Y.A. Lazebnik, et al.; PNAS
92
, 9042 (1995)
Abstract
;
Full Text
Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo:
G.M. Shah, et al.; Anal. Biochem.
227
, 1 (1995), (Review)
Abstract
Detection of poly(ADP-ribose) polymerase and its apoptosis-specific fragment by a nonisotopic activity-western blot technique:
G.M. Shah, et al.; Anal. Biochem.
232
, 251 (1995)
Abstract
C.J. Froelich, et al.; BBRC
227
, 658 (1996)
Abstract
Proteolysis of poly(ADP-ribose) polymerase by caspase 3: kinetics of cleavage of mono(ADP-ribosyl)ated and DNA-bound substrates:
D. D'Amours, et al.; Radiat. Res.
150
, 3 (1998)
Abstract
Towards new neuroprotective agents: design and synthesis of 4H-thieno[2,3-c] isoquinolin-5-one derivatives as potent PARP-1 inhibitors
:
R. Pellicciari, et al.; Farmaco
58
, 851 (2003)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P18493:
PARP-1 (bovine)
Further Categories Containing This Product:
Enzymes
•
Natural Proteins
ALX-202-044
Revised 25-Oct-06
PARP-1 (bovine) (automodified) Standard
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (bovine) (automodified) Standard
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-202-044-R100
100 µl
70.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Isolated from calf thymus.
QUANTITY:
1µg/ml.
FORMULATION:
Liquid.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
Product Description
100µl of automodified PARP-1 (bovine) with enough PAR polymer for 10 immunoblot assays with Prod. No.
ALX-210-890
.
Product Specific Literature References
Immunodot blot method for the detection of poly(ADP-ribose) synthesized in vitro and in vivo:
E.B. Affar, et al.; Anal. Biochem.
259
, 280 (1998)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Swiss-Prot link P18493:
PARP-1 (bovine)
Further Categories Containing This Product:
Enzymes
•
Natural Proteins
ALX-202-046
Revised 25-Oct-06
PARP-1 (bovine) (~98%)
SYNONYMS
Poly(ADP-ribose) Polymerase-1 (bovine) (~98%)
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-202-046-C010
10 µg
250.00 USD
Product Specification
EC:
2.4.2.30
SOURCE/HOST:
Isolated from calf thymus.
CONCENTRATION:
65.2µg/ml
PURITY:
~98% (SDS-PAGE, Western blot)
FORMULATION:
Liquid. In 50mM TRIS-HCl, pH 7.4, containing 1M NaCl, 10mM β-mercaptoethanol and 10mM 3-methoxybenzamide.
SPECIFIC ACTIVITY:
300-400U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1nmol of poly(ADP-ribose) per min. at 25°C.
SHIPPING:
SHIPPED ON DRY ICE
LONG TERM STORAGE:
-80°C
HANDLING:
Avoid freeze/thaw cycles.
Product Specific Literature References
[1]
Poly(ADP-ribose) polymerase is a zinc metalloenzyme:
P. Zahradka & K. Ebisuzaki; Eur. J. Biochem.
142
, 503 (1984)
Abstract
[2]
Erasable blot of poly(ADP-ribose) polymerase:
S. Desnoyers, et al.; Anal. Biochem.
218
, 470 (1994)
Abstract
[3]
Autoantibodies reacting with poly(ADP-ribose) and with a zinc-finger functional domain of poly(ADP-ribose) polymerase involved in the recognition of damaged DNA:
S. Muller, et al.; Clin. Immunol. Immunopathol.
73
, 187 (1994)
Abstract
[4]
Characterization of the execution phase of apoptosis in vitro using extracts from condemned-phase cells
:
Y.A. Lazebnik, et al.; J. Cell Sci. Suppl.
19
, 41 (1995)
Abstract
[5]
Studies of the lamin proteinase reveal multiple parallel biochemical pathways during apoptotic execution:
Y.A. Lazebnik, et al.; PNAS
92
, 9042 (1995)
Abstract
;
Full Text
[6]
Methods for biochemical study of poly(ADP-ribose) metabolism in vitro and in vivo:
G.M. Shah, et al.; Anal. Biochem.
227
, 1 (1995), (Review)
Abstract
[7]
Detection of poly(ADP-ribose) polymerase and its apoptosis-specific fragment by a nonisotopic activity-western blot technique:
G.M. Shah, et al.; Anal. Biochem.
232
, 251 (1995)
Abstract
[8]
Granzyme B/perforin-mediated apoptosis of Jurkat cells results in cleavage of poly(ADP-ribose) polymerase to the 89-kDa apoptotic fragment and less abundant 64-kDa fragment:
C.J. Froelich, et al.; BBRC
227
, 658 (1996)
Abstract
[9]
Purification of the death substrate poly(ADP-ribose) polymerase
:
D. D'Amours, et al.; Anal. Biochem.
249
, 106 (1997)
Abstract
[10]
Characterization of the necrotic cleavage of poly(ADP-ribose) polymerase (PARP-1): implication of lysosomal proteases
:
S. Gobeil, et al.; Cell Death Differ.
8
, 588 (2001)
Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION
Purified by 3-aminobenzamide affinity chromatography (for method see [9]).
Swiss-Prot link P18493:
PARP-1 (bovine)
Further Categories Containing This Product:
Enzymes
•
Natural Proteins
ALX-210-219
Revised 07-Dec-06
Polyclonal Antibody to PARP-1 (197-214)
SYNONYMS
anti-Poly(ADP-ribose) Polymerase-1 (197-214) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-219-R100
100 µl
240.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Contains 0.02% sodium azide.
IMMUNOGEN:
Synthetic peptide corresponding to aa 197-214 (K
197
QLPGVKSE
GKRKGDEVD
214
) of human PARP-1 (poly(ADP-ribose) polymerase-1).
SPECIFICITY:
Recognizes human, bovine and chicken PARP-1. Detects bands of ~116kDa (full length PARP-1) and ~29kDa (apoptosis-induced cleavage fragment) by Western blot.
APPLICATION:
ELISA (1:100’000)
Immunocytochemistry (1:50)
Western Blot (1:10’000-1:15’000)
SHIPPING:
SHIPPED ON BLUE ICE
LONG TERM STORAGE:
+4°C
Product Specific Literature References
Structural and functional analysis of poly(ADP ribose) polymerase: an immunological study:
D. Lamarre, et al.; Biochim. Biophys. Acta
950
, 147 (1988)
Abstract
General Information
During apoptosis PARP-1 is cleaved between Asp
214
and Gly
215
to yield two fragments of 29kDa and 85kDa. This cleavage site is conserved in human, bovine and chicken PARP-1.
Further Categories Containing This Product:
Apoptosis & Necrosis Detection Other Products
•
Polyclonal Antibodies
ALX-210-220
Revised 07-Dec-06
Polyclonal Antibody to PARP-1 (215-228)
SYNONYMS
anti-Poly(ADP-ribose) Polymerase-1 (215-228) PAb
PRODUCT LINE
DNA Regulation / Transcription
PRODUCT CATEGORY
PARP-1
Ordering Information
Product Numbers:
Format:
Size:
Unit Price:
Quantity:
Add To Cart
ALX-210-220-R100
100 µl
240.00 USD
Product Specification
SPECIES CROSSREACTIVITY:
Human
Others
SOURCE/HOST:
From rabbit.
FORMULATION:
Liquid. Contains 0.02% sodium azide.
