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DNA Regulation / Transcription
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ALX-804-103 Revised 13-Dec-04
Monoclonal Antibody to Retinoic Acid Receptor β (Rβ28)
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SYNONYMS anti-RARβ MAb (Rβ28)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Retinoic Acid Receptors / Related Products
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ALX-804-103-C050   50 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Rβ28
ISOTYPE: Mouse IgG2a
FORMULATION: 50µg Lyophilized antibody containing 0.05% sodium azide.
RECONSTITUTION: Reconstitute with 0.1ml of distilled water.
IMMUNOGEN: Synthetic peptide corresponding to C-terminal aa 463-482(P463SVSPSSVENSGVSQSPLLQ482) of mouse RARβ (retinoic acid receptor β).
SPECIFICITY: Recognizes human and mouse RARβ. Does not cross-react with RARγ.
APPLICATION: Western Blot (1-2µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
BLOCKING PEPTIDE: For Blocking Peptide see Prod. No. ALX-166-004.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-104 Revised 03-Mar-05
Monoclonal Antibody to Retinoid X Receptor β (MOK13-17)
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SYNONYMS anti-RXRβ MAb (MOK13-17)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Retinoid X Receptor / Related Products
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ALX-804-104-C100   100 µg 379.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: MOK13-17
ISOTYPE: Mouse IgG1
CONCENTRATION: 2mg/ml
FORMULATION: Liquid. Ascites diluted in PBS containing 0.05% sodium azide.
IMMUNOGEN: Recombinant human RXRβ (retinoid X receptor β).
SPECIFICITY: Recognizes human and mouse RXRβ. Detects a band of ~50kDa by Western blot. Does not cross-react with RXRα or RXRg.
APPLICATION: Gel Super Shift Assays
Immunocytochemistry
Immunoprecipitation
Western Blot (5µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
H-2RIIBP expressed from baculovirus vector binds to multiple hormone response elements: M.S. Marks, et al.; Mol. Endocrinol. 6, 219 (1992) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Immunocytochemical staining of RXRβ in MCF-7 cells overexpressing RXRβ with Prod. No. ALX-804-104 results in strong nuclear staining.
Gel super shift experiments show disruption of vitamin D receptor (VDR)/RXRβ/VDRE (DNA) complex formation when RXRβ was preincubated with Prod. No. ALX-804-104, yet experiments using THR showed a supershift of the thyroid hormone receptor (THR)/RXRβ heterodimer by this product.
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-444 Revised 09-Nov-04
Monoclonal Antibody to Retinoid X Receptor γ (human) (1373)
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SYNONYMS anti-RXRγ (human) MAb (1373)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Retinoid X Receptor / Related Products
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ALX-804-444-C100   100 µg 390.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 1373
ISOTYPE: Mouse IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. Protein G purified antibody in PBS, pH 7.4, containing 0.2% BSA and 15mM sodium azide.
IMMUNOGEN: Synthetic peptide corresponding to aa 213-226 of human RXRγ (retinoid X receptor γ).
SPECIFICITY: Recognizes human RXRγ. Detects a band of ~60kDa by Western blot. Does not cross-react with human RXRα, RXRβ or human retinoic acid receptor (RAR).
APPLICATION: Western Blot (1µg/ml)
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-849 Revised 16-Aug-07
Monoclonal Antibody to RIG-I (Alme-1)
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SYNONYMS anti-RIG-1 MAb (Alme-1)
anti-Retinoic Acid-inducible Gene 1 Protein MAb (Alme-1)
anti-DEAD-box Protein 58 MAb (Alme-1)
anti-Probable ATP-dependent RNA Helicase DDX58 MAb (Alme-1)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY RNA Helicases / Related Products
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ALX-804-849-C100   100 µg 290.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
Mouse
CLONE: Alme-1
ISOTYPE: Mouse IgG1
SOURCE/HOST: Purified from concentrated hybridoma tissue culture supernatant.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIG-I (retinoic acid-inducible gene 1 protein) (aa 201-713).
SPECIFICITY: Recognizes human and mouse RIG-I.
APPLICATION: Immunohistochemistry (paraffin sections)
Immunoprecipitation (1:100)
Western blot (1:1’000)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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General Information
RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
Virology. Sensing viral RNA amid your own: T. Fujita; Science 314, 935 (2006) Abstract
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science 314, 994 (2006) Abstract
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science 314, 997 (2006) Abstract
Related Products
Further Categories Containing This Product:
Antiviral Signalling / Related Products • NF-kB Pathway Other Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Interferons & Interferon Receptors Other Products • Polyclonal Antibodies
 
 
ALX-804-139 Revised 09-May-07
Monoclonal Antibody to RIP2 (human) (Nick-1)
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SYNONYMS anti-Receptor-interacting Protein 2 (human) MAb (Nick-1)
anti-CARDIAK (human) MAb (Nick-1)
anti-CARD-containing Interleukin-1β Converting Enzyme Associated Kinase (human) MAb (Nick-1)
anti-RICK (human) MAb (Nick-1)
anti-RIPK2 (human) MAb (Nick-1)
anti-Receptor-interacting Serine/Threonine Protein Kinase 2 (human) MAb (Nick-1)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-804-139-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Nick-1
ISOTYPE: Rat IgG2a
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIP2 (receptor-interacting protein 2) (aa 1-322).
SPECIFICITY: Recognizes human RIP2.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored as recommended.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-604.
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Product Specific Literature References
Participation of Rip2 in LPS signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
PIDD mediates NF-kB activation in Response to DNA damage: Janssens, et al.; Cell 123, 1079 (2005) Abstract
Participation of Rip2 in lipopolysaccharide signaling is independent of its kinase activity: C. Lu, et al.; J. Biol. Chem. 280, 16278 (2005) Abstract; Full Text
General Information
MANUFACTURER Manufactured by Apotech Corporation.
Further Categories Containing This Product:
Nod-complex / Related Products • Caspase Recruitment Domain [CARD] Proteins / Related Products • Monoclonal Antibodies
 
 
ALX-804-155 Revised 27-Feb-07
Monoclonal Antibody to RIP3 (human) (Rippy-3)
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SYNONYMS anti-Receptor-interacting Protein 3 (human) MAb (Rippy-3)
anti-Receptor-interacting Serine/Threonine Protein Kinase 3 (human) MAb (Rippy-3)
anti-RIP-like Protein Kinase 3 (human) MAb (Rippy-3)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY RIP Kinases
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ALX-804-155-C100   100 µg 360.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: Rippy-3
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RIP3 (receptor-interacting protein 3).
SPECIFICITY: Recognizes human RIP3.
APPLICATION: Western Blot
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 1 year after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
POSITIVE CONTROL: For Positive Control (Western blot) see Prod. No. ALX-840-608.
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General Information
Certain serine/threonine protein kinases, such as ASK1, RIP, DAP, and ZIP kinases, are mediators of apoptosis. Receptor interacting proteins including RIP and RIP2/RICK mediate apoptosis induced by TNF-R1 and Fas, two prototype members in the death receptor family. A novel member in the RIP kinase family was recently identified and designated RIP3 [1-3]. RIP3 contains an N-terminal kinase domain but, unlike RIP or RIP2, lacks the C-terminal death or CARD domain. RIP3 binds to RIP and TNF-R1, mediates TNF-R1 induced apoptosis, and attenuates RIP and TNF-R1 induced NF-κB activation. Overexpression of RIP3 induces apoptosis and NF-κB activation. The messenger RNA of RIP3 is expressed in a subset of adult tissues [2,3]. Meanwhile RIP4 has been identified and initially characterized. These recent data suggest that RIP4 is involved in NF-κB and JNK signalling and that caspase-dependent processing of RIP4 may negatively regulate NF-κB-dependent pro-survival or pro-inflammatory signals [4].
MANUFACTURER Manufactured by Apotech Corporation.
General Literature References
[1] Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB: P.W. Yu, et al.; Curr. Biol. 9, 539 (1999) Abstract
[2] RIP3, a novel apoptosis-inducing kinase: X. Sun, et al.; J. Biol. Chem. 274, 16871 (1999) Abstract; Full Text
[3] Mouse receptor interacting protein 3 does not contain a caspase-recruiting or a death domain but induces apoptosis and activates NF-kappaB: N.J. Pazdernik, et al.; Mol. Cell. Biol. 19, 6500 (1999) Abstract; Full Text
[4] RIP4 (DIK/PKK), a novel member of the RIP kinase family, activates NF-kappa B and is processed during apoptosis: E. Meylan, et al.; EMBO Rep. 3, 1201 (2002) Abstract; Full Text
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Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-581 Revised 19-Feb-07
Monoclonal Antibody to RNase L (human) (2E9)
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SYNONYMS anti-RNase L (human) MAb (2E9)
anti-Ribonuclease L (2',5'-Oligoisoadenylate Synthetase-dependent) (human) MAb (2E9)
anti-2-5A-Dependent RNase (human) MAb (2E9)
anti-Ribonuclease 4 (human) MAb (2E9)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Regulation / Transcription Other Products
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ALX-804-581-C100   100 µg 190.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 2E9
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
PURITY DETAIL: Affinity purified.
FORMULATION: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
IMMUNOGEN: Recombinant human RNase L.
SPECIFICITY: Recognizes human RNase L. Detects a band of ~83kDa by Western blot. Does not cross-react with mouse RNase L.
APPLICATION: ELISA
Western Blot (Alkaline phosphatase/ECL detection)
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry (paraffin sections)
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
2-5A-dependent RNase molecules dimerize during activation by 2-5A: B. Dong & R.H. Silverman; J. Biol. Chem. 270, 4133 (1995) Abstract; Full Text
Elevated levels of 2',5'-linked oligoadenylate-dependent ribonuclease L occur as an early event in colorectal tumorigenesis: L. Wang, et al.; Clin. Cancer Res. 1, 1421 (1995) Abstract
Alternative function of a protein kinase homology domain in 2', 5'-oligoadenylate dependent RNase L: B. Dong & R.H. Silverman; Nucleic Acids Res. 27, 439 (1999) Abstract
Germline mutations in the ribonuclease L gene in families showing linkage with HPC1: J. Carpten, et al.; Nat. Genet. 30, 181 (2002) Abstract
An infectious retrovirus susceptible to an IFN antiviral pathway from human prostate tumors: B. Dong, et al.; PNAS 104, 1655 (2007) Abstract; Full Text
General Information
RNase L is an endoribonuclease regulated by 2-5A synthetase (2-5A). It is a 741 aa cytoplasmic and mitochondrial protein in human. RNase L functions in the antiviral and antiproliferative activities of interferons (IFN) and in multiple apoptotic pathways, standing in the first line of the innate cellular defense mechanism induced by the IFN pathway.
In its native state, the N-terminal half of RNase L functions as a repressor of the ribonuclease domain in the C-terminal half. Upon binding with high affinity to 2-5A molecules, the inactive monomeric form of RNase L is induced to dimerize into its catalytically active form.
Since mutations in RNase L relate to an increase incidence of prostate cancer, the activation of RNase L directly or indirectly suppresses prostate tumorigenesis and/or metastasis. The 2-5A/RNase L pathway is implicated in mediating apoptosis in response to viral infections and to several types of external stimuli. The fungal ribonuclease, α-sarcin, the RNA modyfing enzyme, ricin A chain, and UV light each lead to ribotoxic stress responses involving damage to the 3-end of the large ribosomal RNA. These treatments activate c-jun N-terminal kinases (JNKs). JNK activation in response to UV irradiation has been linked to apoptosis through Bax (requires caspase 3 activity, appearance of cytosolic cytochrome C indicating involvement of mitochondria).
General Literature References
Implications for RNase L in prostate cancer biology: R.H. Silverman; Biochemistry 42, 1805 (2003), Review Abstract
Effects of RNase L mutations associated with prostate cancer on apoptosis induced by 2',5'-oligoadenylates: Y. Xiang, et al.; Cancer Res. 63, 6795 (2003) Abstract
Further Categories Containing This Product:
Monoclonal Antibodies
 
 
ALX-804-644 Revised 29-May-07
Monoclonal Antibody to RORα (human) (6E8)
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PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Nuclear Receptors Other Products
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ALX-804-644-C050   50 µg 180.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: 6E8
ISOTYPE: Mouse IgG1
CONCENTRATION: ~580µg/ml
PURITY DETAIL: Protein G-affinity purified.
FORMULATION: Liquid. In PBS, pH 7.6. Contains no preservatives.
IMMUNOGEN: Recombinant human RORα.
SPECIFICITY: Recognizes human RORα1-4. The epitope is located at aa 84-105 of RORα4.
APPLICATION: Electrophoretic Mobility Shift Assay (EMSA)
Immunoprecipitation
Western Blot (2µg/ml)
Optimal conditions must be determined individually for each application.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: +4°C
USE/STABILITY: Stable for 12 months after receipt when stored at +4°C.
HANDLING: Do not freeze.
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Product Specific Literature References
Intracellular localization of RORalpha is isoform and cell line-dependent: A. Aschrafi, et al.; Biochim. Biophys. Acta 1763, 805 (2006) Abstract
Overexpression, refolding, and purification of polyhistidine-tagged human retinoic acid related orphan receptor RORalpha4: A. Lechtken, et al.; Protein Expr. Purif. 49, 114 (2006) Abstract
Further Categories Containing This Product:
Polyclonal Antibodies
 
 
ALX-803-318 Revised 24-Apr-08
Monoclonal Antibody to RUNX3 (human) (R3-6E9)
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SYNONYMS anti-Runt-related Transcription Factor 3 (human) MAb (R3-6E9)
anti-CBF-α3 (human) MAb (R3-6E9)
anti-Core-binding Factor α3 Subunit (human) MAb (R3-6E9)
anti-AML-2 (human) MAb (R3-6E9)
anti-Acute Myeloid Leukemia 2 Protein (human) MAb (R3-6E9)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Transcription Factors Other Products
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ALX-803-318-C100   100 µg 320.00 USD Add To Cart
Product Specification
SPECIES CROSSREACTIVITY:
Human
CLONE: R3-6E9
ISOTYPE: Mouse IgG1
CONCENTRATION: 1mg/ml
FORMULATION: Liquid. In PBS containing 0.02% sodium azide.
IMMUNOGEN: Recombinant human RUNX3 (runt-related transcription factor 3) (aa 191-300).
SPECIFICITY: Recognizes human RUNX3. Weakly cross-reacts with mouse RUNX3.
APPLICATION: Immunohistochemistry (paraffin sections (2µg/ml)) (see [1])
Immunoprecipitation
Western Blot (0.2µg/ml (see [1]))
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
HANDLING: After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Specific Literature References
[1] RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization: K. Ito, et al.; Cancer Res. 65, 7743 (2005) Abstract; Full Text
[2] RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer: Q.C. Lau, et al.; Cancer Res. 66, 6512 (2006) Abstract; Full Text
[3] Targets of genome copy number reduction in primary breast cancers identified by integrative genomics: W. Chen, et al.; Genes Chromosomes Cancer 46, 288 (2007) Abstract
General Information
RUNX (Runt-related transcription factor) family members play important roles in normal developmental processes and carcinogen