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DNA Regulation / Transcription
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ALX-201-236 Revised 17-Aug-06
NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
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SYNONYMS NADK (NT truncated) (active) (human) (recombinant) (His)
Poly(P)/ATP NAD Kinase (NT truncated) (active) (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-201-236-C050   50 µg 260.00 USD Add To Cart
Product Specification
EC: 2.7.1.23
SOURCE/HOST: Produced in E. coli. Human NADK (aa 64-446) is fused at the N-terminus to a His-tag.
QUANTITY: Sufficient for the preparation of >10µmol of NADP from NAD and ATP in 1ml of a 10mM solution.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
PURITY DETAIL: Free of any nucleotide converting side-activities.
FORMULATION: Liquid. Ammonium sulphate suspension.
RECONSTITUTION: Prepare working aliquots by centrifugation and dissolve the pellet in 50mM TRIS/HCl pH 7.5, 150mM NaCl, 1mM DTT.
SPECIFIC ACTIVITY: ≥3U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1µmol of NADP per min.
SHIPPING: SHIPPED ON BLUE ICE
SHORT TERM STORAGE: +4°C
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months when stored at +4°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural and functional characterization of human NAD kinase: F. Lerner, et al.; BBRC 288, 69 (2001) Abstract
Further Categories Containing This Product:
Enzymes • Kinases Other Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-237 Revised 12-Aug-08
NMNAT1 (human) (recombinant) (His)
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SYNONYMS NMN Adenylyltransferase 1 (human) (recombinant) (His)
Nicotinamide Mononucleotide Adenylyltransferase 1 (human) (recombinant) (His)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-201-237-C100   100 µg 390.00 USD Add To Cart
Product Specification
MW: 33.3kDa (monomer)
EC: 2.7.7.1
SOURCE/HOST: Produced in E. coli. Full length human NMNAT1 is fused at the N-terminus to a His-tag.
CONCENTRATION:

~1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
SPECIFIC ACTIVITY: 10-20U/mg
APPLICATION: Well suited for the synthesis of NAD due to high specific activity (10-20U/mg) and high substrate selectivity compared to NMNAT3 (human) (recombinant) (Prod. No. ALX-201-238).
Note: When loss of activity is observed add 10mM DTT to the working buffer and incubate for at least 15 min. before the assay.
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months after receipt when stored at -20°C. Unstable in solutions containing less than 250mM salt.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase: M. Emanuelli, et al.; J. Biol. Chem. 276, 406 (2001) Abstract; Full Text
Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis: M. Schweiger, et al.; FEBS Lett. 492, 95 (2001) Abstract; Full Text
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9HAN9: NMNAT1 (human)
Related Products
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-238 Revised 18-Mar-08
NMNAT3 (human) (recombinant)
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SYNONYMS NMN Adenylyltransferase 3 (human) (recombinant)
Nicotinamide Mononucleotide Adenylyltransferase 3 (human) (recombinant)
PRODUCT LINE Signal Transduction
PRODUCT CATEGORY NAD+ Metabolism / Related Products
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ALX-201-238-C100   100 µg 390.00 USD Add To Cart
Product Specification
MW: 30.3kDa (monomer)
EC: 2.7.7.1
SOURCE/HOST: Produced in E. coli. Full length human NMNAT3 is fused at the N-terminus to a His-tag.
CONCENTRATION: 0.8mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM sodium phosphate, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.
APPLICATION: Well suited for the synthesis of NAD analogs due to lower substrate selectivity compared to NMNAT1 (human) (recombinant) (Prod. No. ALX-201-237).
SHIPPING: SHIPPED ON BLUE ICE
LONG TERM STORAGE: -20°C
USE/STABILITY: Stable for at least 3 months after receipt when stored at -20°C.
HANDLING: Avoid freeze/thaw cycles.
Product Specific Literature References
Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis: X. Zhang, et al.; J. Biol. Chem. 278, 13503 (2003) Abstract; Full Text
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem. 280, 36334 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q96T66: NMNAT3 (human)
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Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-243 Revised 26-Apr-06
ETS1 (human) (recombinant)
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SYNONYMS c-ets-1 Protein (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY Transcription Factors Other Products
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ALX-201-243-C050   50 µg 210.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 2mg/ml
PURITY: ≥90% (SDS-PAGE)
APPEARANCE: Liquid. In 50mM TRIS-KOH, pH 7.0, containing 6mM magnesium dichloride, 50mM potassium chloride, 5µM zinc dichloride, 2mM DTT and 0.1% NP40.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
USE/STABILITY: For consistent results use a fresh aliquot for each experiment.
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
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General Information
The Ets family of transcription factors is known to control the expression of genes critical for cellular proliferation, differentiation, and transformation. They activate transcription by interaction with purine-rich sequences in promoter/enhancer regions of various genes. Differential expression of Ets genes is particularly associated with embryonic development of the central nervous system. Previous studies have implicated Ets transcription factors in apoptosis; however, exact mechanisms remain unclear. The mammalian Ets1 gene encodes a set of nuclear phosphoproteins ranging in size from 42 to 54kD.
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Recombinant Proteins / Fusion Proteins
 
 
ALX-201-244 Revised 27-Mar-07
Endonuclease G (human) (recombinant)
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SYNONYMS EndoG (human) (recombinant)
PRODUCT LINE Cell Death / Apoptosis / Autophagy
PRODUCT CATEGORY HtrA2 / Omi, ARTS, EndoG / Related Products
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ALX-201-244-C020   20 µg 190.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in E. coli. Recombinant human endonuclease G is fused to a His-tag at the C-terminus.
CONCENTRATION: 0.4mg/ml
PURITY: ≥90% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 8.0, containing 500mM sodium chloride, 0.05% Tween 20, 5mM β-mercaptoethanol, 500mM imidazole and 20% glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
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Further Categories Containing This Product:
DNA Endonucleases / Related Products • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-249 Revised 18-Mar-08
FEN-1 (human) (recombinant)
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SYNONYMS Flap Structure-specific Endonuclease 1 (human) (recombinant)
MF1 (human) (recombinant)
Maturation Factor 1 (human) (recombinant)
DNase IV (human) (recombinant)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY DNA Endonucleases / Related Products
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ALX-201-249-C010   10 µg 260.00 USD Add To Cart
Product Specification
MW: ~42kDa
SOURCE/HOST: Produced in E. coli.
CONCENTRATION: 1mg/ml
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 25mM TRIS-HCl, pH 7.5, containing 50% glycerol, 1mM DTT, 1mM EDTA and 0.02% Nonidet P40.
SHIPPING: SHIPPED ON DRY ICE
SHORT TERM STORAGE: -20°C
LONG TERM STORAGE: -80°C
USE/STABILITY: Stable for at least 4 weeks when stored at -20°C.
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Flap endonuclease 1: a novel tumour suppresser protein: G. Henneke, et al.; TIBS 28, 384 (2003) Abstract
The Fen1 extrahelical 3’-flap pocket is conserved from archaea to human and regulates DNA substrate specificity: E. Friedrich-Heineken and U. Hubscher; Nucl. Acids Res. 32, 2520 (2004) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P39748: FEN-1 (human)
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Recombinant Proteins / Fusion Proteins
 
 
ALX-201-250 Revised 18-Mar-08
PARP-1 (human) (recombinant) (high purity) (His)
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SYNONYMS Poly(ADP-ribose) Polymerase-1 (human) (recombinant) (high purity) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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ALX-201-250-C010   10 µg 240.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf21 cells. Human full-length PARP-1 is fused to a His-tag.
CONCENTRATION: 0.5mg/ml.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY: ≥1’200U/mg protein.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
Transcriptional coactivation of nuclear factor-kappaB-dependent gene expression by p300 is regulated by poly(ADP)-ribose polymerase-1: P.O. Hassa, et al.; J. Biol. Chem. 278, 45145 (2003) Abstract; Full Text
Acetylation of poly(ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-kappaB-dependent transcription: P.O. Hassa, et al.; J. Biol. Chem. 280, 40450 (2005) Abstract; Full Text
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P09874: PARP-1 (human)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-251 Revised 18-Mar-08
PARP-2 (mouse) (recombinant) (high purity) (His)
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SYNONYMS Poly(ADP-ribose) Polymerase-2 (mouse) (recombinant) (high purity) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-2
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ALX-201-251-C010   10 µg 240.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf21 cells. Mouse full-length PARP-2 is fused to a His-tag.
CONCENTRATION: 1mg/ml.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link O88554: PARP-2 (mouse)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-252 Revised 18-Mar-08
PARP-3 (human) (recombinant) (high purity) (His)
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SYNONYMS Poly(ADP-ribose) Polymerase-3 (human) (recombinant) (high purity) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-3
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ALX-201-252-C010   10 µg 240.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf21 cells. Human full-length PARP-3 is fused to a His-tag.
CONCENTRATION: 1mg/ml.
PURITY: ≥98% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link Q9Y6F1: PARP-3 (human)
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-253 Revised 18-Mar-08
PARP-1b (human) (recombinant) (active) (His)
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SYNONYMS Poly(ADP-ribose) Polymerase-1b (human) (recombinant) (active) (His)
short-form PARP-1 (human) (recombinant) (active) (His)
sPARP-1 (human) (recombinant) (active) (His)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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ALX-201-253-C020   20 µg 240.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf21 cells. Human PARP-1b (short-form PARP1; sPARP-1) is fused to a His-tag.
CONCENTRATION: 0.5mg/ml.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: After opening, prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
Product Specific Literature References
Characterization of sPARP-1. An alternative product of PARP-1 gene with poly(ADP-ribose) polymerase activity independent of DNA strand breaks: F.R. Sallmann, et al.; J. Biol. Chem. 275, 15504 (2000) Abstract
General Information
BACKGROUND/TECHNICAL INFORMATION Swiss-Prot link P09874: PARP-1 (human)
General Literature References
Nuclear ADP-ribosylation reactions in mammalian cells: where are we today and where are we going?: P.O. Hassa, et al.; Microbiol. Mol. Biol. Rev. 70, 789 (2006) Abstract
Further Categories Containing This Product:
Enzymes • Recombinant Proteins / Fusion Proteins
 
 
ALX-201-254 Revised 18-Mar-08
PARP-1 (E988K mutant) (human) (recombinant) (Control)
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SYNONYMS Poly(ADP-ribose) Polymerase-1 (E988K mutant) (human) (recombinant) (Control)
PRODUCT LINE DNA Regulation / Transcription
PRODUCT CATEGORY PARP-1
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ALX-201-254-C010   10 µg 180.00 USD Add To Cart
Product Specification
SOURCE/HOST: Produced in Sf21 cells. Human full-length inactive mutant E988K of PARP-1 is fused to a His-tag.
CONCENTRATION: 0.5mg/ml.
PURITY: ≥95% (SDS-PAGE)
FORMULATION: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 50mM imidazole, 0.2% NP-40 and 10% glycerol.
SPECIFIC ACTIVITY: 0.5% of wild type PARP-1.
SHIPPING: SHIPPED ON DRY ICE
LONG TERM STORAGE: -80°C
HANDLING: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Product Specific Literature References
The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function: P.O. Hassa, et al.; J. Biol. Chem. 276, 45588 (2001) Abstract; Full Text